development administration and rating process in response to above given problems of the final achievement test the writer recommends some suggestions for improvement of the test design and administration
... stress face the conflicting problem of maintaining some degree of photosynthesis while minimizing water loss Stomatal control inresponseto varying moisture stress is the first and perhaps the most ... conprovide an integrated responseto conditions (see 1988 and references therein) In addiDaie, tion, starch is synthesized inthe chloroplast and sucrose is synthesized inthe cytosol, and their relative ... stomatal and nonstomatal responses require direct measurement ofthe various components of nonstomatal responses to differentiate the relative importance of these responses to stress (Epron and...
... agarose gel, andthe band was excised, extracted and cloned into a TOPO-TA vector for sequencing The sequence ofthe 3¢-UTR was obtained by primer walking using the gene-specific forward primer ... neuropeptides andthe putative cDNA sequence of PO-CHH that is translated into a neuropeptide inthe PO The location of intrinsic multipolar cells and structure of nerve branches inthe PO indicate that these ... hypothermia and hyperthermia, in an attempt to define their functions To address these questions, we cloned the fulllength cDNA of PO-CHH and identified the presence ofthe neuropeptide forms in the...
... vegetation The objectives ofthe present study were i) to quantify the radial growth responseof trees to various interventions which were intended to improve each of these factors, that is, draining of ... parts inthe spring of 1974 One part remained untouched; the other was drained by digging ditches, about 10 to 20 m away from one another Some measurements indicated that the corresponding lowering ... Student’s t -test involving the treated trees andthe corresponding control trees Finally, mean RESULTS Each ofthe results presented hereafter refers tothe BAI ofthe trees of a given treatment...
... was absent from the outer region In order to determine whether these differences in expression in vivo were reflected in different responses inherent inthe cells present in inner and outer meniscus, ... mediate the HIF1α induction of P4Hα(I) To determine whether thein vitro responseof meniscus cells to hypoxia was relevant to their behaviour in vivo, we analyzed intact meniscus tissue and found ... (post-normalization to β-actin) to P4Hα(I) gene expression (post-normalization to β-actin) in 20% oxygen in monolayer culture of meniscus cells inthe presence and absence ofthe hypoxia inducible factor-1α inhibitor...
... ofthe lysosome andthe proteasome on GLUT-1 downregulation To determine the contribution ofthe major protein degradation pathways tothe decrease inthe total GLUT-1 protein content found in ... especially due tothe presence inthe OA joint of proinflammatory catabolic cytokines such as IL-1β, which has been shown to induce GLUT-1 expression both inthe present study (Figure 2) andin other studies, ... contributes to cartilage degradation in late OA In another study, exposure to either low or high glucose concentrations induced insulin-like growth factor-1 The aim ofthe present study was therefore to...
... follicles andthe maximum diameter ofthe largest follicle during the period before ram introduction are presented in Table After introduction ofthe rams, the diameter ofthe largest follicle andthe ... and ovulation and conception rates in cyclic ewes primed with intravaginal sponges containing 60 mg of MAP for 12 days While Control ewes remained near rams during all the period before joining, ... higher in E-O ewes than in NE-O and NE-NO ewes in samples obtained before introduction ofthe rams, there were no significant differences in LH basal concentrations The mean values forthe number of...
... bleomycin challenge further increased cathepsin K mRNA and protein levels both in wild-type mice andin transgenic mice A more precise understanding ofthe factors governing cathepsin K regulation in ... added tothe pellet and incubated over ice for 30 with intermittent mixing After incubation, the lysate was centrifuged again at 13,000 rpm for 30 andthe supernatant containing the protein was stored ... antiinflammatory agents and immune modulators have proved to be minimally effective in limiting alveolitis and modifying the course ofthe disease, thus underlining the urgent need for novel therapeutic...
... "in- vitro" producers of IFN-γ and TNF-α cytokines inresponseto P aeruginosa They contribute at the same extent as the predominant αβ subset tothe percentage of cytokine-positive cells in total ... function and integrity [6,7] but they have been also implicated insome pathogenic cytokine disregulation anddevelopmentof airway inflammation [11] Their cytokine production inresponseto bacterial ... proliferation in relation to clinical severity of patients (data not shown) Discussion The present study is the first, to our knowledge, that determines the "in vitro" intracytoplasmic cytokine response to...
... population residing in main family dwellings (households) of Spain and is mainly performed by the Ministry of Health and Consumer Affairs andthe National Statistics Institute (Instituto Nacional ... conceived ofthe study, and participated in its designand coordination and helped to draft the manuscript All authors read and approved thefinal manuscript Authors’ information None Competing interests ... participated inthedesignofthe study and performed the statistical analysis EPM carried out the acquisition ofthe data, analysis and interpretation of data She has been involved in revising it...
... (c) to perform that experiment using microbial growth tests, andto analyse the results; and (d) on the basis of these to design, perform and evaluate the next experiment, the whole continuing in ... causal linkages The Manchester Interdisciplinary Biocentre (MIB) Many ofthe kinds ofproblems described above, and certainly the solutions being developed to attack them, require the input of ideas ... activities: information retrieval, to gather relevant texts; information extraction, to identify and extract a range of specic types of information from texts of interest; and data mining, to nd associations...
... (Grx1 and Grx2) and five monothiolic glutaredoxins These isoforms differ in their location andresponseto oxidative stress, among other factors [9,11,18–22] Evidence indicates that Grx2 is the main ... CPYC domain appears inthe inner core of most of them To corroborate the results shown above, we tested whether deglutathionylation by Grx2 is increased when its entry into the catalytic chamber ... of them being totally exposed and others slightly buried but still solventaccessible All ofthe other cysteine residues are either buried inthe skeletal structure or exposed tothe internal catalytic...
... before the end ofthe respective incubation conditions, in case of hypoxic labeling by plunging a spatula carrying the appropriate quantity in dried form into the cell culture medium To stop incubations ... a continuous flow of humidified artificial air containing 5% (v/v) CO2 for normoxic incubations, and with 0.02% O2, 5% CO2, and Ar to 100% for hypoxic gassing For gassing, the equipment andthe procedures ... while maintaining the respective incubation conditions during labeling and processed for measuring the ratio between acid-insoluble 3H and 14C radioactivity reoxygenation, incorporation of radioactivity...
... S Gillings et al providing a rationale forthe use of combinations of MEK1 ⁄ inhibitors and PI3K–PKB pathway inhibitors Indeed, rapamycin, an inhibitor of mammalian target of rapamycin (mTOR) ... proliferation, survival and transformation [56,57] The importance ofthe BCR–ABL tyrosine kinase inthe survival of CML cells led tothedevelopmentofthe tyrosine kinase inhibitor imatinib (STI571, Gleevec), ... ERK2, and (b) the presence of a unique hydrophobic inhibitor binding pocket adjacent tothe Mg ⁄ ATP-binding site that exhibits little homology to other kinases and explains the high degree of specificity...
... Cells on the wound edge were divided equally into three sectors, including the front sector between the nucleus andthe leading edge The Golgi inthe front sector was determined to be inthe polarized ... migration inresponseto wound healing Results Cas is required forthe polarization of migrating cells To examine the role of Cas inthe establishment of cell polarity during cell migration, we performed ... of Cdc42 activity during cell polarization Multiple tyrosine residues inthe substrate-binding domain of Cas are phosphorylated inresponseto various extracellular stimuli, including integrin-mediated...
... MAPK and triggers its dissociation from the cytoskeletal protein GKAP, therefore releasing it from the cytoskeleton into the cytoplasm [3] Our aim in this study was to gain a better understanding ... concentrations indicated Cells were exposed to 0.5 M sorbitol for 60 min, and then incubated for h in sorbitol-free medium (in the continued absence or presence of inhibitor) Endogenous levels of hDlg ... and then incubated for h in sorbitol-free medium (in the continued absence or presence ofthe inhibitor) Endogenous levels of hDlg were analysed by immunoblotting (lower) andthe percentage of...
... proteins involved in various cellular processes, including inhibition of apoptosis Hypoxia has been reported to inhibit apoptosis induced by tumor necrosis factor-related apoptosisinducing ligand ... enhancement in apoptosis relative to cells expressing BRCA1 inthe absence of TRAIL and a three-fold enhancement of apoptosis over that induced by TRAIL inthe presence ofthe empty vector Thus, the ... hypoxia and TRAIL effect a change in BRCA1 localization, only TRAIL induces apoptosis The inability of hypoxia to cause apoptosis was not surprising, considering the number of reports demonstrating...
... introduced inthe tanks, probably due to adsorption of atrazine tothe surfaces ofthe tanks and possibly due to removal by the fish themselves [46] Moreover, the authors suggested that someof ... cypermethrin At the same time, there was a decrease inthe amount of free amino acids coincident with changes that occurred inthe transamination process, again, related tothe formation of nitrogenous ... genes in common to both the ADI and glyphosate response SSH libraries These were glutamine synthase, ATP synthetase, b-1,3-glucan binding protein andsome housekeeping genes such as tubulin and...
... closest to brain endothelial cells, wrapping around them, joined to them by gap junctions, and interfacing with them by peg -and- socket structures [2,3] These cells are also essential forthe induction ... involved in activation of brain microvascular pericytes by LPS Another interesting finding of our study is related tothe production of important signaling molecules, cytokines and chemokines by ... macrophage-colony stimulating factor, eotaxin, CCL-3 and CCL-4 To further characterize pericyte immune capacity, we determined the effect of LPS on the release of cytokines and chemokines The results (Figure...
... predictors in one ofthe other models, were included tothe model and stayed inthe model To examine whether disease severity is a predictor forresponsethe WOMAC global baseline score was included ... [4-6] One ofthe key issues for evidence-based and cost-effective medicine is the detection and proof ofthe effects of a particular intervention In fact, the ability of an instrument to detect ... lowest level of quality of life at baseline (EQ-5D index and VAS) had the greatest improvementin WOMAC andinthe transition scale (Table 2) Table Predictors ofresponseto NSAIDs on the univariate...
... W– seedlings were allowed to dry to a water content between 7% and 10% At the end ofthe first soil-drying cycle W– seedlings were watered to runoff on 20 and 23 Sept, before beginning the second ... (RWCa) and inverse of osmotic potential at full turgor (Ψπ100) The x- and y-coordinates ofthe first data point ofthe linear portion ofthe PV curve corresponded to relative water content at the ... to a series of two soil-drying cycles of 51 and 38 days (W–), respectively, beginning on 23 July and extending to 24 October Moderate water-stressed seedlings (W–) were watered to runoff at the...