cloning sequencing and expression of a novel goose type lysozyme gene with chitinase ra chic activity from the moderately thermophilic bacterium ralstonia sp a 471

Báo cáo khoa học: Cloning, characterization and localization of a novel basic peroxidase gene from Catharanthus roseus potx

Ngày tải lên : 23/03/2014, 09:21

... AY032675 DQ650638 AY206412 AY206413 AF244923 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA At5g40150 NA NA NA NA NA NA NA NA NA NA NA NA At5g05340 NA NA NA NA NA NA NA NA NA Unpublished Unpublished ... pairs were: #GSP-4 and #PFLF1 (lanes and 2); #GSP-2 and #GSP-4 (lanes and 4); #GSP-2 and #PFLR-1 (lanes and 6); and #PFLF-1 and #PFLR-1 (lanes and 8) (B) Schematic organization of the CrPrx gene ... Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana Gene 288, 129–138 Tanaka S, Ikeda K, Ono M & Miyasaka H (2002) Isolation of several anti-stress genes...

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Báo cáo khoa học: Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase pdf

Ngày tải lên : 16/03/2014, 18:20

... cDNA for expression was cloned by another RT-PCR with primers fullMJEforMQ (GCATGCAGGGTGATAAAAA TCACTTTGTA) and fullMJErev (AAGGATCCATAA TATTTTTGCGAAATC), adding restriction sites for SphI and ... were designed for the rapid ampliﬁcation of cDNA ends (RACE) (RACEfor: GTGA CAGCTTTCATGCCTGG; and RACErev: ATCCTGT CCGTTGTTGTAAAC) 5¢- and 3¢-RACE was performed using the SMART II system (BD ... on the plant species and the tissue analysed and vary from : (JA/MeJA) in Arabidopsis leaves [4] to about 10 : in tomato ﬂowers [38] For tomato cell suspension cultures, a JA/MeJA ratio of almost...

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Báo cáo khoa học: cDNA cloning and characterization of a novel calmodulinlike protein from pearl oyster Pinctada fucata potx

Ngày tải lên : 16/03/2014, 23:20

... arrest and temporal storage of calcium ions as CaM Fig Identification of calcium binding activity of oyster CaM and CaLP on nitrocellulose membrane after SDS electrophoresis A, B and C are an autoradiograph ... 5¢-CTAATACGACTCACTATAGGGC AAGCAGTGGTAACAACGCAGAGT-3¢ and 5¢-CTAAT ACGACTCACTATAGGGC-3¢) and a reverse specific gene primer LSG1 In the second round of PCR reaction, the first round of PCR products then were used as the ... molecular weight of approximately 18 kDa and 14 kDa, respectively, whereas in the absence of calcium, the apparent molecular mass was 25 kDa and 17 kDa, respectively The shift in the band upon calcium...

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Báo cáo Y học: Cloning and expression of two novel aldo-keto reductases from Digitalis purpurea leaves potx

Ngày tải lên : 18/03/2014, 01:20

... plant AKRs indicated that DpARs belong to the same subfamily as the alfalfa enzyme [16] and form a separate cluster from the other plant AKR4 (Fig 2) Southern blot analysis revealed that DpARs ... overnight at 65 °C with a random primed 32 P-labelled cDNA probe (SmaI fragment of 900 bp from DpAR1) The membranes were ﬁnally washed in · NaCl/ Cit, 0.1% SDS for 20 at 65 °C Autoradiography of the ... clones of 1324 and 1319 bp, designated DpAR1 and DpAR2, respectively The nucleotide sequences of DpAR1 and DpAR2 have been submitted to the EMBL database and are available under accession numbers AJ309822...

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Báo cáo Y học: Identiﬁcation and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is speciﬁcally modulated in thyroid cells by cAMP pot

Ngày tải lên : 23/03/2014, 21:20

... (1977) Analysis of singleand double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange Proc Natl Acad Sci USA 74, 4835–4838 Savonet, V., Maenhaut, C., ... right panel, the total mRNA loaded on the gel is controlled by acridine orange staining of the ribosomal RNAs 28S and 18S variety of adult and fetal tissues was used (data not shown) The results ... hybridization At the time of harvest, the cells, in subconﬂuent monolayers, were rapidly scraped from the dishes in M guanidinium monothiocyanate Separation and puriﬁcation of total RNA was performed...

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Cloning and characterization of a novel kelch like gene in zebrafish

Ngày tải lên : 03/10/2015, 20:57

... only if the radioactivity count was still too high The membrane was wrapped with a Saran polyethylene to keep the membrane moist An X-omat (Kodak) film was placed and autoradiographed at -80°C ... primers and used as templates for probe labelling with the Random Primers DNA Labelling System (Gibco BRL, USA) Random priming involved the use of random hexamers that anneal at random sites along the ... identification of mutants by positional cloning and the candidate gene approach, the genetic maps have been useful in comparative studies between zebrafish and other vertebrate genomes By comparing the...

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Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

Ngày tải lên : 18/02/2014, 16:20

... 757–778 AAG AGC GCA ACT GAT AGT GCA TCC GCC ATC GAC GCA ATT AGC CTT GCT AGC AGT ACG AGG 613–630 822–839 706–723 466–483 AAG AGC AAA GAT GAT AGT GAT TAC GCC ATC CCA CAG ATT AGC ATC AAT AGC AGT CAG ... sections of the branchial plume and of the trophosome as shown in Fig 2A The branchial plume is composed of a central obturaculum, mainly made of extracellular matrix, supporting many branchial ﬁlaments ... pseudoobscura, the mosquitoes A aegypti and A gambiae, the clam T gigas and larval sequences from the cnidarian F scutaria Finally, we chose an outgroup comprising a- CAs from a cyanobacteria (Nostoc sp. )...

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Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the speciﬁc and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf

Ngày tải lên : 17/03/2014, 03:20

... only with EC (Fig 3A) These results indicated that the b-aryl ether cleavage enzyme accumulated and was stable in the extracellular fraction The extracellular fraction of 2BW-1 generated abundant ... localization of the b-aryl ether cleavage activity, we prepared a hyphae fraction (HP), an extracellular fraction (EC), a cytoplasmic fraction (CY) and a membrane fraction (M) from cultures of 2BW-1 ... water The incorporation of 18O from radiolabeled water was not observed with guaiacol It was clear that the b-aryl ether cleavage enzyme catalyzed the addition of two molecules of H2O (at Ca and...

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$Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx$

Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx

Ngày tải lên : 30/03/2014, 20:20

... Thanumalayan S, Muralikrishna B, Rangaraj N, Karande AA & Parnaik VK (1999) Colocalization of intranuclear lamin foci with RNA splicing factors J Cell Sci 112, 4651–4661 11 Gueth-Hallonet C, Wang ... imidazole and the bound His6-ISP36 was then eluted with m urea buffer containing m imidazole and stored at )80 °C until use Preparation of rabbit anti-ISP36 sera An anti-ISP36 serum was raised ... intervals at a setting of 220 V and a 960 lF capacitor using a Gene Pulser and Capacitance Extender (Bio-Rad, Hercules, CA, USA) After the pulse, the electroporated cells were incubated with an...

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báo cáo hóa học: " The design and testing of a novel mechanomyogram-driven switch controlled by small eyebrow movements" docx

Ngày tải lên : 19/06/2014, 08:20

... Scholarship, Natural Sciences and Engineering Research Council of Canada and the Canada Research Chairs program The authors acknowledge Mr Ka Lun Tam for his implementing the hardware interfaces, and Mr ... demonstrated by qualitative participant feedback and the trends in response time Further, as a control site, the frontalis muscle is broad and has a large surface area on the forehead, thus offering ... the time] The experimental protocol was approved by the hospital and university research ethics boards, and was in compliance with the Declaration of Helsinki Alves and Chau Journal of NeuroEngineering...

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báo cáo hóa học: "Initial development and testing of a novel foam-based pressure sensor for wearable sensing" doc

Ngày tải lên : 19/06/2014, 10:20

... evaluations, and drafted the manuscript SB created the foam sensors, participated in the prototype pilot evaluations, and drafted the manuscript BS participated in the project organization and ... were attached at the outer edge of the garment at the apex of each shoulder (above the subject's axilla) The sensors measured 1.5 × 2.0 × 0.5 cm Data was gathered with the subject seated, and the ... physiological functions, and body state from body-garment interactions Importantly, the sensor maintains the attractive structural properties of foam, consistent with the objectives of wearability and...

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Báo cáo y học: "The identification and characterization of a novel protein, c19orf10, in the synovium" docx

Ngày tải lên : 09/08/2014, 10:20

... to the maintenance of synovial inflammation, aggravating the destruction of cartilage and bone and stimulating the development of the pannus Because FLSs can exhibit significant phenotypic changes ... containing the c19orf10 c19orf10 gene is indicated on the ideogram The area containing the c19orf10 gene is expanded and the base-pair positions are indicated The location of a microsatellite marker ... similar patterns of reactivity in a comparative staining analysis These antibodies were all of the IgM class and this may be attributable to the fact that there is 91% identity between the predicted...

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báo cáo khoa học: " The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway" pptx

Ngày tải lên : 12/08/2014, 03:20

... GGGTTTCATATGACTATCGGAGCTCGTGAT CGGGATCCCTAGAAGTCATACAAGCATTT TTTTTAAGCTAACACGAGAC TCTCATAGGAGCTGTAATTG TAAAATGGACGATCAGTATC TTATGTTCAGATTTGGACTC TACTTTCTACAACGAGCTTC ACATGATTTGAGTCATCTTC GGGTTTCATATGAAGATCGAGGTGAGAGAA ... CGGGATCCTTAGATATCATATAGGAACTTGC ATATTCACGACGACTCCGATAGCGGTATCG CACGTCGGCTTCGACTGTAGGTCGACT CACGAGACCAAGTCAATGCACTCAAAGGA GATTCGGGCACTTAAACGTATGAGCCCC CGTGGACTATCAGACGATCAACCATCC TCGTCCGTCAGTAGCCACGTACAGTATC ... quinate transferase from Coffea canephora (ABO77956); Cca_HCT, shikimate/quinate hydroxycinnamoyltransferase from Coffea canephora (ABO47805); NP_179497 and NP_200592, Arabidopsis thaliana genes...

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Báo cáo khoa học: "Identification and characterisation of a novel anti-viral peptide against avian influenza virus H9N" pps

Ngày tải lên : 12/08/2014, 04:21

... CATAGAATTCGCAAAAGCAGGAGT 3' 5' TATCGCTCGAGAGTAGAAACAAGGAG 3' 5' ATTTAAGGTACCGACAGCCATGGA 3' 5' ATGCTGCTCGAGTATACAAATGTTGC 3' 5' AGCCTGGAATTCATGAAAAAATTA 3' 5' ATCGAACTCGAGATTTTCAGGGAT 3' 5' AGGGCTGGCGGTTGGGGGTTATTCGC ... AGGGCTGGCGGTTGGGGGTTATTCGC 3' 5' GAGTCACTTTAAAATTTGTATACAC 3' 5' GATGTTAACGATACCAGCC 3' 5' GCGTGAATGTAAGCGTGAC 3' 5'ATTTAAGGATCCGAGAGCCATGGA 3' 5'ATGCTGCTCGAGTTATATACAAATGTTGC 3' 5'CATAGAATTCGCAAAAGCAGGAGT 3' ... 5'CATAGAATTCGCAAAAGCAGGAGT 3' 5'TATCGCTCGAGAGTAGAAACAAGGAG 3' 5'AGCCTGGAATTCATGAAAAAATTA 3' 5'CTCACTCGAGACATTTTCAGGGA 3' aIn all of the above mentioned oligonucleotides, the suffixes F and R refers Forward and Reverse...

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Báo cáo y học: "Quantifying the major mechanisms of recent gene duplications in the human and mouse genomes: a novel strategy to estimate gene duplication rates" docx

Ngày tải lên : 14/08/2014, 08:20

... research TAGs are tandemly arrayed genes that belong to the same gene family There are sometimes spacers within a TAG, which are genes that not belong to the same family as the TAG members Similar ... be the rate achieved We used the total weight W (the percentage of duplicated genes that are either TAGs or retrogenes) to transform the sum of Ru and Rr into the overall gene duplication rate ... reliable In fact, using the rates of duplication by retroposition alone to estimate the overall rates of gene duplication also gives an estimate that is of the same magnitude as the combined rate...

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TARGETING ACUTE PHOSPHATASE PTEN INHIBITION AND INVESTIGATION OF A NOVEL COMBINATION TREATMENT WITH SCHWANN CELL TRANSPLANTATION TO PROMOTE SPINAL CORD INJURY REPAIR IN RATS

Ngày tải lên : 24/08/2014, 09:14

... for vascular quantification using a general rat endothelial cell marker, and calculation of the vessel-positive area was performed in the ipsilateral and contralateral gray matter in vehicle- and ... beginning as early as the beginning of the 20th century with the work of Ramon y Cajal (Ramon y Cajal, 1928), and reinvigorated several decades later (David and Aguayo, 1981, Bray et al., 1987), ... towards the more important goals in graduate research training and for all the advice and suggestions to attain them This guidance will always be remembered and appreciated iii ABSTRACT Chandler...

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Development and evaluation of a novel nanoparticulate delivery system of arsenic sulfides

Ngày tải lên : 11/09/2015, 16:03

... physician and founder of chemotherapy, developed an organic arsenical, Salvarsan (Arsphenamine), which was effective in treating tuberculosis and syphilis Arsphenamine was the standard therapy for ... Trivalent arsenics are more toxic than the pentavalent ones [Duker AA et al., 2005] The activity and toxicity of an arsenical could also vary with the physical state of the compound, and the rate ... hypergranular form (AML FAB M3) and the microgranular variant (AML FAB M3v) The morphological diagnosis is confirmed by the APL specific translocation t(15;17) This translocation generates a fusion...

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Identifcation and stablization of a novel 3d hepatocyte monolayer for hepatocyte based applications

Ngày tải lên : 12/09/2015, 08:19

... hepatoblastoma cell line, are cultured in modified dialysis cartridges The cells are located in the extracapillary space of the hollow fiber, and are separated from plasma by the capillary membranes ... enzymes), and the requirement of relatively low amount of test materials The disadvantages of hepatocytes-based screening are the lack of host factors and the lack of non-parenchymal cells Drug metabolism ... diagram of the procedure of grafting PAAc on the PET film upon argon plasma activation and UV-induced polymerization…………………………………… 45 Fig Schematic diagram of ligands conjugation onto PET-PAAc...

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Identification and characterization of a novel heart reactive autoantibody in systemic lupus erythematosus possible serological marker for early myocardial dysfunction

Ngày tải lên : 14/09/2015, 12:42

... incidence was found in Iceland and Japan and highest in USA and France The overall prevalence was the lowest in Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden ... Schematic illustrating concept of strain and strain rate (SR) Adapted from "Clinical applications of strain rate imaging." (Yip et al 2003) 46 Figure Example of strain rate (SR) image Adapted from ... aboriginal populations in Australia and New Zealand report higher prevalence and incidence in this population than in Australian Caucasians Both racial and genetic differences, and more complex social...

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Roles of siRNAs and miRNAs in host responses to virus infection identification and characterization of a novel viral suppressor of RNA silencing

Ngày tải lên : 16/09/2015, 17:11

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