a divergent subgroup of the hsp70 family hsp105ais expressed constitutively and induced by various forms of stress

Báo cáo Y học: Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases pdf

Ngày tải lên : 08/03/2014, 22:20

... recombinant allergen after affinity purification using the mAb IG12 [18]. The natural allergen Phl p 1 was also found to be capable of degrading a synthetic substrate at a papain- cleavage site after ... degradation of the full-length allergen and accumulation of a truncated  15-kDa fragment at about pH 4.5 (Fig. 3A) . This sharp band lacked the N-terminal peptide, as the N-glycosylated Asn residue ... are one of the earliest lineages of eukaryotic cells, and the Giardia protease is the earliest known branch of the cathepsin B family [23]. Its phylogeny confirms that the cathepsin B lineage evolved in...

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Báo cáo khoa học: A structural overview of the PDI family of proteins docx

Ngày tải lên : 15/03/2014, 23:20

... reveals interaction between ERp57 and the tip of the calreticulin P-domain. Proc Natl Acad Sci USA 99, 1954–1959. 53 Satoh M, Shimada A, Keino H, Kashiwai A, Nagai N, Saga S & Hosokawa M ... oxidoreductase activity. The b and b¢ domains, although structurally similar to thioredoxin, do not contain catalytically active cysteines. Instead, the b and b¢ domains appear to act as spacers, and are often ... signiﬁcant differences in orientation of their a and a domains. The catalytic cysteines (orange) of the 4 °C yeast PDI structure face each other. (B) The b and b¢ domains (gray) of human PDI are...

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Báo cáo khoa học: Characterization of ubiquitin-like polypeptide acceptor protein, a novel pro-apoptotic member of the Bcl2 family pptx

Ngày tải lên : 17/03/2014, 10:20

... residue of Ubi-L and the lysine of Bcl-G. The 33.5 kDa Ubi-L adduct was digested by V8 protease and subjected to MALDI-MS analysis. The data in the first column are the mass values obtained experimentally, whereas ... 2 and 4 contain an aliquot from the hydroxylapatite purification step; lanes 1 and 2, silver-stained; lanes 3 and 4, immunostained with anti-(Ubi-L). Mobilities of the 33.5 kDa Ubi-L adduct and ... third column are those calculated from the V8 protease fragmentation of the gene products of Bcl-G and Ubi-L. The fourth column indicates the number of the first and last amino acid of the identified...

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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

Ngày tải lên : 23/03/2014, 03:20

... shrimp Litopenaeus vannamei KSLILCVLLAGAFA APSRKPTFRRGLNK CAA60129 2,10 KSLVLCLLLAGAFA APSRKPTFRRGLNK CAA75310 KSLVLCLLLAGAFA APSRKPTFRRGLNK CAA75311 Signal crayfish Pacifastacus leniusculus MKTLVFCLLLVGALA ... Forward All PaTrys Rv 5¢-AGTGACCAGCACAGATAGC-3¢ 220–238 Reverse PaTry4 Rv 5¢-GTGGATCCAGTGTTCGTCAT-3¢ Reverse All PaTrys Fw 5¢-CCGTGCCCATCGTGTCTGA-3¢ 551–569 Forward EF1 -a Fw 5¢-CCAGTAGACAAACCACTTCG-3¢ ... partial trypsin cDNA sequences. Obtaining 5¢ and 3¢ ends by Rapid Amplification of cDNA Ends (RACE) Using total RNA as the template, the 5¢ and 3¢ ends of trypsin mRNAs were amplified using 5¢ and...

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Báo cáo khoa học: A hydrophobic segment within the C-terminal domain is essential for both client-binding and dimer formation of the HSP90-family molecular chaperone pptx

Ngày tải lên : 23/03/2014, 20:22

... pKT25 kan .The DNA fragments encoding tentative middle domain and C-terminal one of human GRP94 were ampliﬁed by PCR andtheninsertedinanXbaI/BamHI site of both pUT18C amp and pKT25 kan . The DNA fragment ... Oral and Maxillofacial Surgery and 2 Division of Oral Molecular Biology, Department of Developmental and Reconstructive Medicine, Course of Medical and Dental Sciences, Nagasaki University Graduate School ... binding activity. The binding activity of the C-terminal domain (100%) or its mutated forms toward the middle- C-terminal domains was quantiﬁed as the b-galactosidase activity of the bacterial two-hybrid...

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báo cáo hóa học: " A psychometric evaluation of the PedsQL™ Family Impact Module in parents of children with sickle cell disease" pptx

Ngày tải lên : 18/06/2014, 18:20

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A contrastive study of connotation of the vietnamese zodiac animals in english and vietnamese idioms and proverbs

Ngày tải lên : 26/11/2013, 13:29

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A contrastive analysis of semantic and pragmatic features of the words denoting birds in english and vietnamese

Ngày tải lên : 26/11/2013, 13:30

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A study of politeness strategies in requests in the course book ''streamline english departures and connections'' by b hartley & p viney

Ngày tải lên : 14/12/2013, 00:41

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Tài liệu Báo cáo khoa học: SREBPs: physiology and pathophysiology of the SREBP family ppt

Ngày tải lên : 18/02/2014, 13:20

... reductase SREBP-2 NADPH Pyruvate Acetyl-CoA Citrate acetyl-CoA Oxaloacetate Oxaloacetate ACL ACC HMG-CoA HMG-CoA synthase Citrate Malonyl-CoA Palmitate Malate Mitochondria FAS ACC SCD Fatty acid ... T, Ishikawa M, Kumadaki S, Matsuzaka T, Nakagawa Y, Yahagi N, Nakakuki M, Hasty AH et al. (2008) Palmitate impairs and eicosapentaenoate restores insulin secretion through regulation of SREBP-1c ... Yahagi N, Yamamoto T, Ishikawa M, Watanabe K, Matsuzaka T, Nakagawa Y, Takeuchi Y, Kobayashi K, Takahashi A et al. (2008) Cyclin-depen- dent kinase inhibitor, p21WAF1 ⁄ CIP1, is involved in adipocyte...

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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

Ngày tải lên : 18/02/2014, 16:20

... novel C-terminal actin-binding domain Thirumaran Thanabalu 1,2 , Rajamuthiah Rajmohan 2 , Lei Meng 2 , Gang Ren 4,5 , Parimala R. Vajjhala 4 and Alan L. Munn 1,3,4,6 * 1 Institute of Molecular and Cell ... and growth at elevated temperature, on the one hand, and in cortical actin-patch polarization, on the other hand, are at least partially distinct [23]. However, there may still be a functional ... in Table 2. The rabbit polyclonal GFP-spe- ciﬁc antiserum was a gift from J. Kahana and P. Silver (Dana Farber Cancer Center, Boston, MA). The anti-actin mAb was MAB1501 from Chemicon International...

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Tài liệu Báo cáo khoa học: Proteasome involvement in the degradation of the Gq family of Ga subunits pptx

Ngày tải lên : 20/02/2014, 03:20

... of the Gq class Ga subunits, namely Ga q and Ga 16 have a fast rate of degradation. The results impli- cate a prominent role for the proteasome pathway in down regulation and basal turnover of ... representative autoradio- graphies of whole SDS ⁄ PAGE gels loaded with the 35 S immunoprecipitates and the arrowheads indicate the position of Ga q and Ga 16 . The molecular mass standards are indicated. ... Johansson et al. Proteasome degradation of G q proteins Proteasome involvement in the degradation of the G q family of Ga subunits Bente B. Johansson, Laura Minsaas and Anna M. Aragay Department...

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Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc

Ngày tải lên : 21/02/2014, 03:20

... 5¢- ATGAGCTCTGCTTTCCTTCCGGGGA-3¢ S1 5¢- ATTGAGCTCACCAGGAGGGCAGGAGG-3¢ mEts-R* 5¢-GACACCTGTCGGTAACCCTTAAAGCC-3¢ mGATA* 5¢- CGGAGTCGCCTAAGGAGAGATGGAGA-3¢ mE-box* 5¢- AGGGTTCCCGATCGGTGTCTGAGAGA-3¢ mEts-F* ... 5¢- GTGGTACCAGTAGCAGCCGCCGCAAG-3¢ K3 5¢- ATGGTACCGGGCTTTAAGGGTTCCCG-3¢ K2 5¢- ATGGTACCGGAAGGAAAGCAGAGCCC-3¢ K1 5¢- ATGGTACCTGGTGATCCAGGGCTTGC-3¢ Sac 5¢- CCGTTCCGAGCTCCGAGCAC-3¢ S3 5¢- ATGAGCTCGGGAACCCTTAAAGCCCG-3¢ S2 ... Ltd. (Toyama, Japan). The library was prepared by the cleavage of the cap structures of mRNA with Tobacco acid pyrophosphatase followed by ligation with a synthetic oligoribonucleotide (5¢-GUUGCGUUACAAGGUACGC CACAGCGUAUGAUGCGUAA-3¢)...

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Báo cáo khoa học: MR solution structure of the precursor for carnobacteriocin B2, an antimicrobial peptide fromCarnobacterium piscicola Implications of the a-helical leader section for export and inhibition of type IIa bacteriocin activity pdf

Ngày tải lên : 07/03/2014, 15:20

... These investigations were supported by the Natural Sciences and Engineering Research Council of Canada, the Alberta Heritage Foundation for Medical Research, CanBiocin Ltd (Edmonton, AB), and the Canada Research ... structural basis of the inhibition of the antimicrobial activity of CbnB2 by the leader and to assist future analysis of the interaction of preCbnB2 with its ABC transporter protease, it is essential ... N-terminus and C-terminus are indicated, as are the residues at the start and ﬁnish of each a- helix. The position of the double glycine motif where cleavage occurs during maturation of the bacteriocin...

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Báo cáo khoa học: Secondary transporters of the 2HCT family contain two homologous domains with inverted membrane topology and trans re-entrant loops docx

Ngày tải lên : 07/03/2014, 17:20

... vice versa within the OPA family, the AAT family, the ST3 families and the DAACS1 family. In case of the AAT and DAACS1 families, the percentage observed hits and the distribu- tion are as observed ... C-terminal halves of the OPA, AAT, and DAACS families and the N- and C-terminal halves of ST3 (open bars), between the N-terminal halves of ST3 and the C-terminal halves of ST3 (grey bars), and ... N- and C-terminal halves of all the sequences in ST3 and involves blast searches of each sequence in the database against the whole database. Unrelated families of secondary transporters of the...

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Báo cáo khoa học: Biochemical properties of the human guanylate binding protein 5 and a tumor-speciﬁc truncated splice variant Mark Wehner and Christian Herrmann doc

Ngày tải lên : 15/03/2014, 10:20

... such as dynamin and the antiviral protein, Mx. Because of similarities in the molecular architecture of GBPs and members of the dynamin family, GBPs are classiﬁed as a part of the dynamin superfamily. ... Kurosaki M, Kanazawa N, Tanabe Y, Koyama T, Takeda Y, Nakagawa M, Kaki- numa S, Sekine Y et al. (2006) Expressional screening of interferon-stimulated genes for antiviral activity against hepatitis ... superfamily: conserved structure and molecular mechanism. Nature 349, 117–127. 3 Herrmann C, Martin GA and Wittinghofer A (1995) Quantitative analysis of the complex between p21ras and the Ras-binding...

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Family Life, Reproductive Health, and Population Education: Key Elements of a Health-Promoting School docx

Ngày tải lên : 22/03/2014, 12:20

... Health Team and Community Advisory Committee are established, members can start the planning process by conducting a situation analysis. It is important to make the analysis manageable and practical ... up-to-date data can help ensure that efforts focus on the health and developmental needs of the target population. Concerns and perceptions vary by age and gender and by demographic and socio-economic ... inadequate and limited services and programmes for males or females, and then take a lead in developing and carrying out actions that address these issues. Case Study In 11 African countries, approximately...

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Protecting Children''''s Health In A Changing Environment - Report Of The Fifth Ministerial Conference On Environment And Health.pdf potx

Ngày tải lên : 22/03/2014, 16:22

... championed the cause of the environment and health at a time when it was not fashionable, the challenges and responses in this area are now at the centre of the global health agenda. Nevertheless, ... copied in Kazakhstan. • A campaign by students in Austria raised awareness of poor public transport alternatives to the private car, and increased by 50% the number of pupils and teachers who ... Republic Denmark Estonia Finland France Georgia Germany Greece Hungary Iceland Ireland Israel Italy Kazakhstan Kyrgyzstan Latvia Lithuania Luxembourg Malta Monaco Montenegro Netherlands Norway Poland Portugal Republic of Moldova Romania Russian Federation San Marino Serbia Slovakia Slovenia Spain Sweden Switzerland Tajikistan The former Yugoslav Republic of Macedonia Turkey Turkmenistan Ukraine United...

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Báo cáo khoa học: The yeast stress response Role of the Yap family of b-ZIP transcription factors The PABMB Lecture delivered on 30 June 2004 at the 29th FEBS Congress in Warsaw potx

Ngày tải lên : 30/03/2014, 16:20

... further substantiated by computational interactome data that predict their interaction [63]. Interestingly, the observation that Yap4 and Yap6 are induced by a variety of unrelated forms of environmental ... responses are at play [1]. Speciﬁc forms of stress such as heat shock and some forms of oxidative stress [2] demand the activation of the heat shock factor (HSF), a modular protein consisting of a helix–turn– helix ... in the thioredoxin and gluta- thione systems, and other antioxidants such as catalase and superoxide dismutase, among others. Yap1 is therefore central to the adaptive response to oxidative stress, ...

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Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx

Ngày tải lên : 30/03/2014, 16:20

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