aß generation in cell free system utilizing ers in the absence of cytosol

Wu column handbook for size exclusion chromatogrаphy

Ngày tải lên : 21/08/2013, 10:28

... Polyvinyl alcohol gel, 9, 22, 23 Polyvinyl alcohol, SEC of, 364, 420, 559-581 Polyvinyl alcohol-vinylamine, SEC of, 561 Polyvinylamine, SEC of, 561, 576 Polyvinyl formal, SEC, 187 Polyvinylidine ... oil -in- water (o/w) of, 4, 7, particle size of, pore size of, seed particle of, stabilizer of, 4, 168 stirring speed of, 4, 168 of styrene-divinylbenzene, 4, surfactant of, two-phase of, of vinyl alcohol, ... linear velocity of, 40 maximum pressure of, 40 permeability of, 40 swelling factor of, 40 swelling time of, 40 Sephadex columns, 42 bed volume of, 42 characteristics of, 42 column dimension of, ...

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Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry

Ngày tải lên : 05/09/2013, 10:15

... upstream of the sampling site of Kamo River, EDTA was not detected in Kamo River sample On the other hand, in Shikoku region, EDTA was not observed in the river water samples The concentration of EDTA ... Methods for the Examintion of Water would be to perform an inter-laboratory validation study of the proposed method Determination of EDTA in river water samples For the application of this analytical ... concentration in other river water samples The median concentration of EDTA in river water samples was 115 μg/L and the concentrations detected ranged from 18.8 to 443 μg/L In the Kanto region, the highest...

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Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt

Ngày tải lên : 20/02/2014, 23:20

... the presence of two or more allelic copies of the gene of interest) rather than by the presence of low-level amount of contaminating proteins in the immunocomplex In particular, all of the single ... acetylated proteins, removal of the initiator methionine residue and acetylation of the glutamic acid residue is important in regulating the interaction between actin and myosin in the actomyosin complex ... with changes in the organization of the cellular actomyosin complex, whereas actin rearranges into a peripheral ring in preparation for blebbing In this context, the expression of the caspaseresistant...

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Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot

Ngày tải lên : 06/03/2014, 22:21

... that of ETNF-6-H In all cases, it is interesting that the three amino acids are located in the CDRs and are positioned closely together In the case of ECL2B-L, the distance between the Ca atoms of ... heavy chains capable of the degradation of urease in Helicobacter pylori The catalytic antibody light chain, UA15-L, could suppress the number of bacteria infecting the mouse stomach In these catalytic ... which are the sequences of amino acids 2–6 and 5–9 from the N-terminus of hTNF-a This means that the recombinant hTNF-a contains impurities lacking some amino acids near the N-terminus For the reacted...

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Báo cáo khoa học: Probing the unfolding region of ribonuclease A by site-directed mutagenesis potx

Ngày tải lên : 16/03/2014, 18:20

... an increase in kU Furthermore, our results suggest that Leu35, the side chain of which is buried in the interior of the molecule like that of Phe46 (Table 2), is involved in the formation of ... to the transition state, underlining the predominant importance of this region for maintaining the natively folded structure of the RNase A molecule Interestingly, the hydrophobic nature of residues ... disturbance of the secondary structure in A20P/S21PRNase A An increased signal in the CD spectra in the near-UV region of F46Y containing RNase A variants is attributed to the introduction of the additional...

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Quantification of spheroid formation mechanism by size and shape analysis

Ngày tải lên : 11/09/2015, 16:05

... also the highest among the three extruders Extrudates produced by the axial extruder were the densest, thus improving their ability to retain water within the matrix, resulting in a decrease in the ... During extrusion-spheronization, extrudates readily break down into fines and broken cores The breakdown of the extrudates is followed by extensive layering of the fines Extensive layering of fines ... Prof Paul Heng and Dr Celine Liew for their unyielding support and guidance provided during the course of my study I wish to thank the National University of Singapore, for providing me with the...

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Design and control of a small size humanoid robot

Ngày tải lên : 04/10/2015, 10:25

... refer to the x-ordinate of the biped’s hip joint, z-ordinate of the biped’s hip joint, x-ordinate of biped’s swing ankle joint and z-ordinate of biped’s swing ankle joint , and refer to the joint ... convert the Cartesian space to joint space And the rules are obtained through inverse kinematics which would be discussed in the following section 5.1 Inverse Kinematics The aim of inverse kinematics ... Path Planning The rules obtained from inverse kinematics require the inputs of Cartesian coordinates of the hip and swing ankle of the robot, which should be functions of time since these values...

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Phytoremediation of nitrate and bisphenol a by scindapsus auresus

Ngày tải lên : 16/10/2015, 15:37

... phytoremediation of these pollutants without causing detrimental effects to the ecosystem or the plant itself Therefore, the objectives of this study included the determination of the phytoremediation ... of the contaminated waters without incurring high costs (Pilon Smits, 2005) This project focused on the use of Scindapsus aureus (Lindl & André) Engl (Golden Pothos) in the phytoremediation of ... in the study of phytoremediation has assisted in the understanding of the responses and metabolic pathways of plants towards various pollutants up to the cellular level Plant tissue culture involves...

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Housing market of a medium size city in china a case of xiamen

Ngày tải lên : 22/10/2015, 21:08

... roles of the state and market in the housing reform process He uses the concepts of the model of demand and the model of powers to explain the interaction of the state and market in the process of ... development of result findings Finally, Chapter summarizes the main findings of this research study and discusses the implications of the findings It also covers the limitations to the research and offers ... housing market in China; To investigate the determinants of consumers’ preference in the private housing market in a medium-size city in China; 1.3 To examine the implications from the findings...

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Design a Pocket Size Brochure

Ngày tải lên : 01/03/2016, 22:29

... one thing Avoid detail—note the photo is closely cropped and has no background or other distractions Inside (far right), the text is plain; the tiny space needs no subheads, indents or other flags ... one color or value to another— in this case, the blue field to the white field Edges, not ruled lines, yield the sharpest, cleanest, most minimal look Headlines of super-fine Helvetica Neue Ultra ... and order is fay of alm then card whint not oogum or bont thin chack Second spread is twice the width of the first, and its text is transitional between the introduction and main body Right, type...

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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx

Ngày tải lên : 08/03/2014, 16:20

... 214 nm Labeling refers to peak numbers The left inset of (B) shows the spectra of the four main peaks obtained by the diode array detector The right inset of (B) reports the SDS/PAGE of phycobilisomes ... differ from each other by the percentage and type of bilin proteins they contain In order to study the in uence of certain environmental changes on the supramolecular organization of the 1538 L Zolla ... ion of the intact Injection of the total mixture of phycobilisomes from Synechocystis PCC 6803, schematically represented in Fig 1A, into a C4 RP-HPLC system resulted in the separation of the...

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477
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Báo cáo hóa học: " Sensitive and molecular size-selective detection of proteins using a chip-based and heteroliganded gold nanoisland by localized surface plasmon resonance spectroscopy" pptx

Ngày tải lên : 21/06/2014, 04:20

... functions allow the proteins to approach the activated receptor more easily, resulting in the binding of the protein to the activated MPA sites via carboimide coupling to protein -free anime moieties ... group interaction [28-31] The mole fractions of these molecules on the Page of gold nanoisland in the fabrication of the mixed SAM were optimized so as to maximize the sensitivity of the method The ... After annealing procedure of the surface, dimensions of the gold nanoislands were in the range of 40-80 nm in diameter [26,27] The binary SAM containing MPA and DT was fabricated on the gold nanoislands...

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Báo cáo y học: "A step-by-step diagnosis of exclusion in a twin pregnancy with acute respiratory failure due to non-fatal amniotic fluid embolism: a case report" pps

Ngày tải lên : 11/08/2014, 23:21

... while there was no elevation in cardiac enzymes Duplex ultrasound scanning of the lower extremities revealed no thrombosis of the femoral and popliteal veins During her first day in the ICU the ... tomography (CT) scan of the thorax supported the above findings and excluded any case of pulmonary embolism A noradrenaline infusion was started at a low rate (2 µg/minute) during initial resuscitation ... ritodrine infusion was started at a dose of 0.10 to 0.3 mg/minute, given in 1000 ml of normal saline, for 24 hours At 24 hours, uterine contractions were arrested successfully, the ritodrine infusion...

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Báo cáo y học: "A class of human exons with predicted distant branch points revealed by analysis of AG dinucleotide exclusion zones" ppsx

Ngày tải lên : 14/08/2014, 16:20

... for specifying the splice sites [5-7] The 5' splice site consists of a nine-nucleotide consensus containing the invariant GU dinucleotide at the start of the intron At the 3' end of the intron, ... prior to the following: ignoring any AGs in the last 12 nucleotides of an intron in the determination of the AGEZ; and the exclusion from the analysis (for Figure 7) of There are a number of instances ... chance of observing the terminating dinucleotide at each step in the scan was a constant, like that of obtaining a head in the toss of a fair coin, which remains one half irrespective of what...

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The Art of Public Speaking cylinder, piston, water, and fire, is the steam−engine in its most elementary form. For a steam−engine may be defined as an apparatus for doing work by means of heat applied to water; and since raising such a weight as the pisto

Ngày tải lên : 08/08/2012, 17:20

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Immobilization of heavy metals in sediment dredged from a seaport by iron bearing materials

Ngày tải lên : 23/09/2012, 15:38

... diagnosing, treating or immunizing people or animals It can also be the product of the research and testing of biological products 1.1.2  Definition according to the Ministry of Public Health of ... Korean incinerators but they rarely used them as they did not have treatment system Some Vietnamese factories tried manufacturing incinerators but either they could not apply in reality or incinerators ... more supervisors for the processes of containment, collecting, transporting and treating in wastes centres Firstly, in the process of waste classification in hospitals, there should be more strict...

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Báo cáo y học: "Pre-hospital intubation by anaesthesiologists in patients with severe trauma: an audit of a Norwegian helicopter emergency medical service"

Ngày tải lên : 25/10/2012, 09:56

... puts them in a category where the indication for ETI is unclear or at least signifies that other factors, besides severity of injury and GCS, must have influenced the decision to intubate In the ... following ETI Future studies on quality in pre-hospital ETI should investigate and address these issues Our audit was limited to one HEMS system, and the validity of our findings in other systems ... Neurosurgical Intensive Care of the European Society of Intensive Care Medicine Intensive Care Med 1998, 24:1221-1225 Winchell RJ, Hoyt DB: Endotracheal intubation in the field improves survival in patients...

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Báo cáo y học: "Spontaneous Hemoperitoneum Caused By a Diverticulum of the Sigmoid Colon"

Ngày tải lên : 25/10/2012, 10:51

... compared to the small intestine that is formed of the inner circular and outer longitudinal muscle layers The vasa recta, which supply the mucosa and submucosa of the colon, penetrate the circular ... muscle The weakness of the vascular portals in the circular muscle possibly causes mucosal herniation into the subserosa [8] Second, the small diameter of the sigmoid colon causes a high intraluminal ... diverticulosis of the colon I Resting patterns of pressure II The effect of morphine Gut 1964;5:201-13 Painter NS, Truelove SC, Ardran GM, Tuckey M Segmentation and the localization of intraluminal pressure...

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Báo cáo y học: "A cyclic-RGD-BioShuttle functionalized with TMZ by DARinv “Click Chemistry” targeted to αvβ3 integrin for therapy"

Ngày tải lên : 25/10/2012, 11:40

... chemotherapeutic target in the field of targeted therapy.[9] Endocytosis-mediated intracellular trafficking of ligands via the αvβ3 receptor of MCF-7 cells and the αvβ5 integrin receptor into the ... endocytotic internalization of the cRGD into the cytoplasm of αvβ3 and αvβ5 integrin expressing cells we used the confocal laser scanning microscope (CLSM) of the Microscopy Core Facility of the German ... publication the Huang group described inhibiting features of RGD-containing peptides against αvβ3 integrin expressing cells [18] whereas the activity of αvβ5 integrin was already documented from the...

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Báo cáo y học: "Cell Cycle Arrest by a Natural Product via G2/M Checkpoint"

Ngày tải lên : 02/11/2012, 11:12

... decrease of cell population in the G1 phase of the cell cycle (Fig 3) The effect of CKBM on AGS cells appears to be dose-dependent, the higher the dosage the greater the G2/M population increases ... because the cells in the late stage of apoptosis were indistinguishable from the necrotic population The increase in the apoptotic population is in concomitant with the increase in the pre-phase ... Western blot analysis Whether other proteins are also involved in the induction of G2/M cell cycle arrest and inhibition in cell proliferation still need further studies In summary, we have used...

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