6 separation techniques applied to whey and derivatives in the production of cheese

Separation, extraction and concentration processes in the food, beverage and nutraceutical industrie

Ngày tải lên : 20/09/2015, 01:57

... in the hydrostatic pressure However, in the presence of dense gas, the melting point of the solid decreases as the pressure increases owing to the increasing solubility of gas in the solid The ... Isolation of whole casein in the dairy industry 12.6 Separation techniques applied to whey and derivatives in the production of cheese 12.7 Fractionation of individual proteins and peptides ... bar at the top of the highest mountain, up to 1000 bar at the bottom of the deepest ocean Because human beings live on the surface of the globe, the first technologies for the production of various...

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Báo cáo khoa học: Apoptosis-inhibiting activities of BIR family proteins in Xenopus egg extracts ppt

Ngày tải lên : 30/03/2014, 16:20

... apoptosis-inhibiting function, and the further addition of the residue 270–401-containing PEST2 and RING ﬁnger domain resulted in rapid proteolysis Therefore, the apoptosis-inhibiting potential of ... system The BIR (baculovirus IAP repeat, in which IAP stands for inhibitor of apoptosis protein) domain is an evolutionarily conserved zinc-binding motif Many, but not all, proteins containing the ... are distinct proteins rather than the nonallelic isoforms of a single protein in pseudotetraploid X laevis Another cDNA encodes a 401-residue protein containing two N-terminal BIR domains and a...

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Extraction of anthocyanins from industrial purple ﬂeshed sweetpotatoes and

Ngày tải lên : 05/05/2014, 08:39

... amounts of anthocyanins in the storage roots In comparison to other common anthocyanin containing fruits and vegetables, the concentration of anthocyanins in purple-ﬂeshed ISPs are in the same ... Change in AIS was used to represent the change in starch content as a percent of dry matter and was examined in this study to determine the amount of starch converted during hydrolysis In this ... case, the main effects of solvent and extraction temperature, the interaction between solvent and temperature and solvent and solid loading, as well as the full interaction of all three factors...

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Báo cáo hóa học: " Localization studies of two white spot syndrome virus structural proteins VP51 and VP76" docx

Ngày tải lên : 20/06/2014, 01:20

... Identification of VP51 and VP76 by Western blotting The truncated recombinant proteins, VP51p and VP76p, were expressed in Escherichia coli and purified by using NiNTA affinity chromatography, and then ... viral minor capsid proteins The results may facilitate a better understanding of the molecular mechanism of WSSV infection and assembly, or be helpful for the control of virus infection in the future ... 51 and 76 kDa Whilst, Western blotting analysis indicated that the two proteins were exclusively detected in nucleocapsid fraction (Fig 1b) To eliminate the possible interference of the remaining...

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Báo cáo y học: "Eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption" pptx

Ngày tải lên : 13/08/2014, 13:20

... proteins and the egg yolk protein precursor, vitellogenin are synthesized and secreted by the hepatocyte They are transported in blood to the ovary and incorporated into maturing oocytes in female ... is the synthesis of the oogenic proteins, Vtg and Zrproteins E2 is the major estrogen in female fish E2 stimulates the production of Vtg and Zr-proteins in the liver The genes encoding these fish ... determine the processing of primary transcripts into the correct messenger sequences [69,70] Figure shows an order of the molecular mechanisms that lead to the production of Zr-protein and Vtg in the...

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Báo cáo y học: "Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System"

Ngày tải lên : 02/11/2012, 11:08

... and the other half inoculated into 20µl of PCR mix PCR products were sequenced to determine the identity of the interacting proteins 115 Results and Discussion 3.1 Principle of Split-Ubiquitin ... (Cub) but the Nub and Cub have high affinity towards each other and is able to reassemble back together spontaneously into ubiquitin protein When Nub and Cub moieties bind together within a cell, ... resuspended in 5ml 0.5X YPDA/Kanamycin (50µg/ml) and combined into a total volume of 10ml To calculate the mating efficiency, 100µl of a 1: 10 000, 1: 000, 1: 100 and 1:10 dilutions of the mating mixture...

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Comparison of Methods for the Extraction of Bioflocculants from Activated Sludge

Ngày tải lên : 05/09/2013, 09:08

... demonstrated the same mobility in both stains, suggesting that these bands were complexes of protein and saccharide These included the band corresponding to a molecular weight of 103,000 in the steaming-extracted ... (1), the band corresponding to 38,000 in the NaOH-extracted polymer (2), the band corresponding to 24,700 in the SDS-extracted polymer (3), and the bands corresponding to 10,700, 29,200, and 39,000 ... UV-absorbing peak indicated it was composed mainly of nucleic acid In the profile of the NaOH-extracted polymer (B), the elution volume (80 ml) of saccharide corresponded to that of the UV-absorbing...

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Tài liệu Activity 12.1: Risks of No Functional Specification doc

Ngày tải lên : 24/01/2014, 10:20

... along the lines to create the boat The order and directions for folding are documented in the functional specification After completing the above steps, you will discuss the activity with the class ... Risks of No Functional Specification Exercise 1: Creating an Origami Boat ! Create an origami boat Construct an origami boat using the sheet distributed by the instructor Fold the paper along the ... steps, you will discuss the activity with the class Activity 12.1: Risks of No Functional Specification 105 THIS PAGE INTENTIONALLY LEFT BLANK ...

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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the speciﬁcity and relative binding energy of RNA-binding proteins ppt

Ngày tải lên : 18/02/2014, 16:20

... protein mutants and RNA containing cytosine at position )5 (C) The characteristic intramolecular hydrogen bond between the amino group of C5 and the O1P atom of U6 observed in the structure of the ... or absence of a cytosine at position )5 in the RNA A likely explanation for the importance of the )5 cytosine mutation is offered by the observation that the amino group of the cytosine at position ... by the sum of the probabilities of observing the set intermolecular distances deﬁned in the 3D structure, relative to the likelihood of encountering such distance in the dataset of all protein–RNA...

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Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by ﬂuorescence correlation spectroscopy and ﬂuorescence recovery after photobleaching doc

Ngày tải lên : 18/02/2014, 16:20

... diagram of the constructs microinjected into the medaka eggs and transfected into HeLa cells The olvas, nanos and tudor coding sequences were joined to the C-terminal region of the GFP or RFP coding ... transcribed from these constructs in vitro was injected into medaka eggs in the one-cell stage to visualize the localization and to measure the mobility of the components in the PGCs The medaka embryo ... (B) The nuage was seen around the nucleus and came together and apart in this time scale Arrowheads show the assembly and dissociation points measured outside of the nuage in the cytoplasm of the...

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Tài liệu Báo cáo khoa học: Structural bases for recognition of Anp32⁄LANP proteins doc

Ngày tải lên : 18/02/2014, 17:20

... accurate deﬁnition of the C-terminal domain boundary Experimental Study of the interactions of the Anp32a LRR module determination of the dynamic features of the domain in solution, together with a ... parallel to each other are mainly perpendicular to the b-sheet vectors and, therefore, to B0 in the aligned medium These results suggest that interactions with other molecules involving the LRR domain ... known to block the inhibitory PP2A activity of Anp32e in protein extracts [7] Taken together, these ﬁndings limit the region of interaction to the only exposed surface of PP2A-C that contains a...

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Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

Ngày tải lên : 19/02/2014, 00:20

... composed of the sole Glyco_18 domain, the C-terminal tail of C gigas CLPs may not noticeably contribute to the structure and the function of these proteins Interestingly, Cg-Clp1 and Cg-Clp2 C-terminal ... Accumulation of Ym1 ⁄ protein in the mouse olfactory epithelium during regeneration and aging Neuroscience 123, 907–917 13 Recklies AD, Ling H, White C & Bernier SM (2005) In ammatory cytokines induce production ... of larval tissues, such as the velum and the foot, and the remodelling of larval tissues to produce adult tissues (i.e the development of the gills and the production of an adult shell), which...

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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc

Ngày tải lên : 19/02/2014, 02:20

... out in the linear range determined using serial dilutions of samples (Fig 2) Linearity consisting of continuous signal increase and of reproducible glycoprotein patterns was determined in the ... antibodies binding to the core region (i.e amino acids 121–166; C-terminal region) The di-glycosylated band of human PrPC showed the heaviest staining with antibodies binding to the N-terminal region ... The signal intensity of each of the three protein bands was quantiﬁed by densitometry The mean values of the calculated signal intensities were analyzed for each of the N- or C-terminal binding...

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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt

Ngày tải lên : 19/02/2014, 06:20

... downstream of the initiator ATG in exon (Ex1) of the 67 exon-containing htt gene [5] Pathogenesis in HD correlates with the cleavage of mutated htt and the release of an N-terminal fragment bearing the ... Note the presence of the granules in the cytoplasm of the cells Bar, 20 lm (D) The percentage of EGFP-containing cells displaying granules is shown The average percentages, including standard ... was performed to analyze the EGFP-containing inclusions Figure 1F shows that the expression of Hsp70 together with Hdj-1 (Hsp40) did decrease the average size of the EGFP-containing inclusion bodies...

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Tài liệu Báo cáo khoa học: Selection of stably folded proteins by phage-display with proteolysis docx

Ngày tải lên : 19/02/2014, 12:20

... N-terminal domains (N1, N2) of the minor coat protein (g3p) are responsible for binding and infection in E coli Thus, incorporation of a library of target proteins between the N-terminal domain and ... cutting sites are in the ﬂexible loop of the native structure, they could prevent the selection of stable proteins By examining the structures of the proteins studied by the phage-display and ... method should help to provide insights into understanding the forces that stabilize proteins and to designing proteins with new folds A more promising aspect is to combine the computational method...

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Tài liệu Báo cáo khoa học: Subproteomics analysis of Ca2+-binding proteins demonstrates decreased calsequestrin expression in dystrophic mouse skeletal muscle pdf

Ngày tải lên : 19/02/2014, 16:20

... protocols may distort comparative immunoblotting data, it was of interest to re-examine the fate of calsequestrin by studying the entire complement of key Ca2+-binding elements in dystrophindeﬁcient ... tray was then placed on top of a hot plate and the temperature maintained at 90 °C for to aid the staining of protein spots The tray was then placed on a laboratory shaker for a further 10 at ... tracking dye] to achieve a ﬁnal protein concentration of 50 lg of protein per IEF strip for silver staining, hot Coomassie staining, ÔStains-AllÕ labelling or immunoblotting The following running...

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Tài liệu Báo cáo khoa học: "Cross-Domain Co-Extraction of Sentiment and Topic Lexicons" pdf

Ngày tải lên : 19/02/2014, 19:20

... Portland, Oregon ACL Minqing Hu and Bing Liu 2004 Mining and summarizing customer reviews In Proceedings of the tenth ACM SIGKDD international conference on Knowledge discovery and data mining, ... set of patterns which the words wi and wk satisfy We then select the top r candidates as topic seeds Seed Expansion After generating the topic and sentiment seeds, we aim to expand them in the ... DT and the k2 sentiment- and topic- word candidates, and calculate the normalized weights θij ’s for each edge of the graph 6: Reﬁne the scores S1 and S3 of the k2 sentiment and topic word candidates...

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Tài liệu Báo cáo khoa học: "Automatic Extraction of Lexico-Syntactic Patterns for Detection of Negation and Speculation Scopes" pdf

Ngày tải lên : 20/02/2014, 04:20

... list of phrases split into subsets (preceding vs following their scope) to identify cues using string matching The cue scopes extend from the cue to the beginning or end of the sentence, depending ... Statistics of the BioScope corpus The 2nd and 3d columns show the total number of cues within the datasets; the 4th and 5th columns show the percentage of negated and speculative sentences 70% of the ... by matching the path of cue leaf nodes to the root of the rule subtree pattern If an identical path exists in the sentence, the root of the candidate subtree is thus also identiﬁed The candidate...

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Tài liệu Báo cáo khoa học: "Robust Extraction of Named Entity Including Unfamiliar Word" doc

Ngày tải lên : 20/02/2014, 09:20

... context vector Vm of a morpheme m is a vector consisting of frequencies of all possible unigrams 126 The morpheme feature M F (mi ) is a pair of the surface string and the part -of- speech of mi The ... morphemes, and (Asahara and Matsumoto, 2003; Nakano and Hirai, 2004) formalized the task of extracting NEs as a chunking problem of a sequence of characters instead of a sequence of morphemes In this ... Current token is the beginning of a chunk Current token is a middle or the end of a chunk consisting of more than one token Current token is outside of any chunk Actually, we prepare the 16 derived...

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Tài liệu Báo cáo Y học: A Raman optical activity study of rheomorphism in caseins, synucleins and tau New insight into the structure and behaviour of natively unfolded proteins pot

Ngày tải lên : 21/02/2014, 15:20

... that the former is likely to be in the Cterminal half and the latter in the N-terminal half of the protein [29,48±51] Sequence-based structure prediction methods suggest that the caseins are of the ... of strong positive ROA at » 1320 cm)1 to PPII structure relies mainly on the evidence outlined above In view of the close similarity of the ROA spectra of the casein, synuclein and tau proteins ... form of b strand [18] The positive bands at » 1675 cm)1 in the amide I region of the ROA spectra of b- and j-casein, which originate mainly in the peptide C O stretch, are characteristic of disordered...

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