Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 1

... 3 .1 Mia1p in assembly and maintenance of persisent iMTOCs at nuclear envelope 44 3 .1. 1 Lack of microtubule binding protein Mia1p results in fewer interphase Microtubules 44 3 .1. 1 .1 Mia1p is a microtubule ... cytoskeleton 18 18 1. 2.4 .1. 1 Architecture and dynamics of interphase microtubule cytoskeleton 18 1. 2.4 .1. 2 Functions of interphase microtubule cytoskeleton 19 1. 2.4 .1. 2 .1 Maintaining cell morphology 19 1. 2.4 .1. 2.2 ... between Mia1p and Alp14p 10 9 4.5 .1 Mia1p interacts with Alp14p but not Dis1p 10 9 4.5.2 Different aspect of Mia1p and Alp14p functions in microtubule x attachment to the NE 11 0 4.5.3 Lack of Mia1p and...

Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 2

... center and plus-ends facing toward the cell tips Model of maintaining ordered microtubules (from Sawin and Tran, 20 06) is shown in the following cartoon 16 Model of interphase microtubules bundling ... critical role in maintaining cell morphology by binding to the plus ends of growing interphase microtubules in a Tip1p and Tea2p-dependent manner (Chang and Peter, 20 03) When the growing microtubules ... studies in this system identified a partner of D -TACC protein, another centrosomal protein -Minispindles (Msps), making a breakthrough in understanding normal function of TACC protein (Lee et al., 20 01)...

Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 3

... 40μl TE buffer containing RNAse and incubated at 37 oC for 20min DNA was stored at -20oC 2 .3 Construction of knock out mutants and epitope tagging of genes 2 .3. 1 Construction of knock out mutants ... taxol-stabilized bovine brain 3X microtubules (50μg/ml) in the same buffer, followed by washing and immunoblotting withTAT1 anti-tubulin antibodies 2.2.5 Extraction of S .pombe genomic DNA 5ml S pombe cells ... dissolved in PBS and resuspended in 140μl of 1.2M sorbitol disolved in PBS 60μl of protoplasting buffer (50mg/ml Lysing enzyme, 30 mg/ml Zymolase, 1.2 M sorbitol in PBS) was added to the samples and incubated...

Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 4

... kinetochore in metaphase (~1% of cells) and to the spindle midzone during anaphase (~5% of cells) in control cells Ark1p-GFP defined the spindle midzone in 43 % of Mia1p- overexpressing cells, and ... panel) and cells overexpressing Mia1p (right panel) coexpressing the NE marker protein, Uch2p-GFP, and the SPB protein, Sad1p-DSRed 84 3.2.3 Spindles in Mia1p- overexpressing cells are bipolar and ... 3.1.1.1: Microtubule- associated protein Mia1p binds microtubules and is a component of S pombe MTOCs (A) Shown is the maximum projection of the z-stack obtained by epifluorescence imaging of interphase...

Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 5

... interphase microtubule arrays 4 .5. 3 Lack of Mia1p and Alp14p result in failure of iMTOCs establishment Another striking aspect of Mia1p function has been illuminated by lack of large interphase MTOCs in ... that S pombe cells overexpressing the TACC- related protein, Mia1p, were capable of organizing spindle-pole like structures While formation of intranuclear microtubule bundles in interphase S pombe ... understand how detachment of minus ends of microtubules resulted in the absence of detectable iMTOC structures in mia1Δ cells I proved the role of microtubules in establishing the iMTOCs in two...

Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 6

... spindle assembly checkpoint in fission yeast Nat Cell Biol 5, 764 - 766 ; author reply 766 68 Sato, M., and Toda, T (2007) Alp7 /TACC is a crucial target in Ran-GTPasedependent spindle formation in ... Isolation and characterization of AINT: a novel ARNT interacting protein expressed during murine embryonic development Mech Dev 97, 13- 26 66 Samejima, I., Lourenco, P.C., Snaith, H.A., and Sawin, K.E ... 1 461 -1479 22 Drummond, D.R., and Cross, R.A (2000) Dynamics of interphase microtubules in Schizosaccharomyces pombe Curr Biol 10, 766 -775 23 Drummond, D.R., and Hagan, I.M (1998) Mutations in...

Analysis of the multisubunit exocyst complex in schizosaccharomyces pombe

... Mechanisms of cytokinesis in S pombe 11 1.2.2.1 Positioning of the actomyosin ring in S pombe 11 1.2.2.2 Assembly of the actomyosin ring in S pombe 11 1.2.2.2.1 The role of myosin, light chains and ... assembly of the actomyosin ring The key components of the ring are actin and the motor protein myosin II (Schroeder, 1990) Type II myosins are important for the assembly of the actomyosin ring and ... of the actomyosin ring in the subsequent round of mitosis, indicating that the Myo2p spot is important for the assembly of the actomyosin ring 1.2.2.3 A SIN pathway regulating septation In S pombe...

Báo cáo khoa học: Huntington’s disease: roles of huntingtin-interacting protein 1 (HIP-1) and its molecular partner HIPPI in the regulation of apoptosis and transcription pptx

... Htt-interacting protein HIP -1 and its molecular partner HIPPI in the regulation of apoptosis and transcription, the two processes that are altered in HD [7,8] HIP -1 – its interacting partners and ... Conclusions HIP -1 and its interacting partner HIPPI together induce apoptosis by the intrinsic and extrinsic pathways Homer 1c, an interactor of HIPPI, and the wild-type N-terminal Htt, which interacts ... apoptosis induction The effect of HIP -1 may depend not only on the amount of the interacting proteins but also on the afﬁnities of interacting proteins The uncreased expression of caspase -1 observed in...

The investigation of transcription related protein protein interactions of histones in saccharomyces cerevisiae

... THE INVESTIGATION OF TRANSCRIPTION- RELATED PROTEIN- PROTEIN INTERACTIONS OF HISTONES IN SACCHAROMYCES CEREVISIAE ZHAO JIN (M Sc (Molecular Engineering of Biological and Chemical ... Aim of the study To investigate the effects of single point mutation of histones on transcription regulation and the relevant protein- protein interactions in Saccharomyces cerevisiae To study the ... number of interactions detected in larger screens Meanwhile as the design of the split-ubiquitin system poses steric constraints on the two fusion proteins, almost all protein- protein interactions...

The roles of DNA(cytosine 5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals

... proteins E1 protein E1 protein is mainly involved in the replication of the virus by binding to the origin of replication (ori) in the 3’ end of the LCR (Ustav et al., 1991) It is shown to possess ... dependent binding 57 2.22 Cell staining 57 2.22.1 Staining of Hsc70-PCNA 57 2.22.2 Staining of transfected DNMT1 and Hsc70 58 2.22.3 Staining of DNMT1 and YY1 58 2.22.4 Staining of DNMT1 and Hsc70 in ... Cterminus substrate-binding domain The binding site of Hsc70 was localized to aa141-152, probably to the PXPXP sequence at the N-terminus of DNMT1 The presence of an additional 17aa inserted in the...

ROLES OF HUNTINGTIN ASSOCIATED PROTEIN 1 IN INSULIN SECRETING CELLS

... INTRODUCTION 1. 1 β-cell and insulin secretion 1. 1 .1 Diabetes mellitus 1. 1.2 Insulin 1. 1.3 Insulin secretion 1. 1.4 insulin- secreting cell model 1. 1.5 β-cell growth and cell cycle 1. 2 HAP1 10 1. 2 .1 HAP1 background ... of HAP1 in insulin- secreting cells after knockdown of its expression in INS -1 cell line The findings of HAP1 role in INS -1 cell may shed light on the understanding of complicated signaling pathways ... List of Figures Figure The classic signaling pathway for insulin secretion from β -cells Figure Knockdown of HAP1 in INS -1 cells 48 Figure Effects of HAP1 knockdown in INS -1 cell on insulin secretion...

Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc

... role of AP-2a and AP-2e in chondrogenesis Overview of the differentiation stages during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of an ... analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target gene expression, have been performed for AP-2a Up ... [63] Role of AP-2a, AP-2b and AP-2e in chondrogenesis and skeletal development In addition to Sox and Runx transcription factors, members of the AP-2 family also have important functions in chondrogenesis...

Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc

... both a novel C-terminal actin- binding submodule (CABS) containing a novel actin monomer binding verprolin homology C-terminal (VH2-C) domain and a second submodule comprising the previously characterized ... actinbinding domain (this actin- binding domain has not yet been mapped) [23] We name the actin- binding domain that we have identiﬁed VH2-C and VH2-N because it is not yet clear how closely these domains ... Type I myosins can interact via an acidic tail (A) domain with the Arp2 ⁄ complex like Las17p, but they lack an actin- monomer-binding WH2 domain, which in WASP-family proteins (including Las17p) ...

Báo cáo khoa học: Transcript proﬁling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice pdf

... probable role of auxin-responsive genes in reproductive development and abiotic stress signaling in rice Transcript proﬁling of auxin-responsive genes study from our laboratory, the rice coleoptile ... 2009 FEBS M Jain and J P Khurana Transcript proﬁling of auxin-responsive genes Fig Expression proﬁles of auxin-responsive genes in various tissues ⁄ organs and developmental stages of rice A heatmap ... expression proﬁling of auxin-responsive genes in a large number of tissues ⁄ organs and developmental stages in this study To achieve gene expression proﬁling of auxinresponsive genes identiﬁed in this...

Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc

... How the unique features of the E focardii c-tubulins enhance microtubule nucleation by the c-TuRC complex at low temperature, and what these changes imply regarding the mechanism of nucleation? ... that c-T2, but not cT1, participates in the assembly of the mitotic spindle of E focardii and that both isotypes are involved in the nucleation of other microtubule structures Finally, we examined ... located at the plus ends, near the GTP-binding sites, and within the M loops of the E focardii c-tubulins, preserve their microtubulenucleating activities at cold temperatures and ⁄ or confer different...

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