potential roles of endometrial stem progenitor cells in endometrial disorders43
Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
... Sall4B overexpression induces the expression of important HSC regulatory genes in cultured LSK cells Murine Lin-Sca-1+ cells were infected with lentiviruses each containing Sall4 isoform or GFP control, ... removed by washing cells times, then G-CSF (R&D Systems, Minneapolis, MN) was added to cells at a final concentration of 200 ng/ml CFU assay of BM cells Tubes of MethoCult® GF M3434 (Stem Cell Technologies, ... when cells were treated with Sall4 isoforms (Figure 8) ALL of these genes have been reported to play active roles in maintaining short term or long term HSC proliferation In addition, Sall4 isoforms...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... These data suggest Stem- Kine supplementation increases circulation of cells capable of giving rise to hematopoieticlineage cells in vitro Stem- Kine augments circulation of cells with EPC phenotype ... were required to ingest two capsules of Stem- Kine in the morning and two in the evening for 14 days Phenotypic assessment of circulating stem cells Peripheral blood mononuclear cells (PBMC) were ... a peak increase of approximately 53% in the number of CD34 expressing cells and and a 90% increase in CD133 cells in circulation Furthermore, a significant augmentation of cells possessing hematopoietic...
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Báo cáo y học: " Profibrotic potential of Prominin-1+ epithelial progenitor cells in pulmonary fibrosis" docx
... accumulation of bone marrow-derived prominin-1+ progenitors in the lung So far, we have identified two distinct populations of prominin-1 expressing cells in the healthy lung Bone marrow-derived prominin-1+lo ... Harvested cells contained mostly prominin-1-positive cells (A, right) Further, prominin-1positive cells were isolated using magnetic cell sorting and cultured in the presence of different cytokines ... accumulation of prominin-1+ progenitors in the injured lung Inflammation mobilizes and activates local or exogenous stem and progenitor cells We therefore investigated whether bleomycin instillation...
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Báo cáo khoa học: Hypoxia-driven proliferation of embryonic neural stem ⁄ progenitor cells – role of hypoxia-inducible transcription factor-1a ppt
... arresting the growth of most kinds of cells, promote the proliferation of some types of cells There is increasing evidence that mild hypoxia acts as a potent regulator of various types of stem cells ... Generally, neural stem cells were grown to form as neurospheres in vitro The ability of stem cells to form neurospheres is one of the indicators for proliferation of neural stem cells in vitro The ... observations indicate that mild hypoxia may be a useful tool for expansion of some stem cells for clinical use in vitro at low cost However, the molecular mechanisms involved in proliferation of stem cells...
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Báo cáo y học: "Elucidation of the potential roles of matrix metalloproteinases in skeletal biology" pps
... substitution of an arginine by a histidine (R101H) In affected members of both families there was no MMP-2 activity detected in serum by gelatin zymography, in contrast to measurable levels in unaffected ... disruption of the MMP13 gene The strategy employed involved the deletion of the critical zinc-binding region in the catalytic domain that resulted in markedly decreased transcription of the MMP13 ... is increased ascribable to increased generation of osteoblasts Conclusion MMP-13 has critical roles in embryonic development and remodeling of the skeleton in mice These roles are reflected in...
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Báo cáo y học: "Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus" pot
... the amino-terminal two repeats of the death effector domain (DED), it lacks the carboxy-terminal half of the proteolytic domain Caspase 8L binds to the Fas-associating protein with death domain ... and blocks the binding of caspase to FADD by competiive inhibition [39] Indeed, in hematopoietic stem cells and leukemic cells, the elevated expression of caspase 8L protects these cells from Fas-mediated ... reduction of adhesive properties of CPCs One could logically consider the in vitro binding of CPCs to fibronectin as a reflection of the capacity of CPCs to bind to damaged endothelium in vivo...
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Báo cáo y học: "Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells" pdf
... involvement of interferon-gamma (IFN-γ) via its induction of indoleamine 2,3-dioxygenase (IDO), an enzyme involved in the catabolism of tryptophan, an essential amino acid required for protein ... with increasing passage number) We speculate that increased passaging not only eliminates tissue-plastic adherent inflammatory cells (monocytes), but also aids in reducing pro-inflammatory cytokine ... species inhibitresponse Tonsil-derived mesenchymal progenitor cells (T-MPCs) of T cells neic as well as phytohemaglutinin (PHA)-induced proliferative response in a dose-dependent manner regardless of...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... and sequestration of progenitor cells Increased number of circulating progenitor cells has been shown in tissue injury animal models [26,27] as well as in clinical settings including burn, post-cardiac ... remained in the circulation, showing a times enrichment of Lin- PCs in the pneumonia lungs Few of these Lin- PCs migrated into the airspaces (approximately 3.1% of all the cells remaining in the ... remain constant (as shown in the BM homing study by Szilvassy and colleagues [38]), increasing the number of infused progenitor cells will increase the number of these cells that home to injured...
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Báo cáo y học: "Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage" potx
... signal-enhancing system based on biotin in terms of a clearly lower background All antibodies were diluted in PBS containing 5% bovine serum albumin The signal was developed by incubation with ... of the osteogenic lineage, as assessed by the detection of alkaline phosphatase, was more pronounced in CD166+ cells, indicating more progenitor cells in CD166+ than CD166- cells (Figure 5c,d) ... mesenchymal progenitor cell staining shows an absence of a positive signal, supporting the specificity of the abovenamed CD166 staining Magnifications: 100 × (large micrograph), 400 × (insets) Results of...
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Báo cáo y học: "The change and effect of endothelial progenitor cells in pig with multiple organ dysfunction syndromes" ppt
... general anesthesia was induced by intravenous injection of mg/kg etomidate (Braun) Anesthesia was maintained by continuous intravenous injection administration of ketamin hydrochloride (5 to ... of endothelial progenitor cells The attached spindle cells at different points were all positive for taking up Dil-ac-LDL and UEA-1 Co-staining cells revealed that more than 90% of adherent cells ... stroke output The results of blood serum examination were monitored The main observed indexes included alanine aminotransferase, aspartate aminotransferase, creatinine, blood urea nitrogen, white...
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Báo cáo y học: " Effect of erythropoietin on level of circulating endothelial progenitor cells and outcome in patients after acute ischemic stroke" ppsx
... gastro-intestinal tract bleeding during aspirin therapy Clopidogrel was used in patients intolerant to aspirin therapy Other commonly used drugs included statins, angiotensin converting enzyme inhibitors ... Contrary to the findings of our study, the results of one recent clinical trial did not find an additional benefit of EPO in improving clinical outcome of patients with acute IS undergoing tPA therapy ... assessment of the serial changes in circulating level of EPCs in IS patients Blood samples were also obtained in control subjects who participated in a health screening program in our Health Clinic...
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Báo cáo y học: "Translational control plays a prominent role in the hepatocytic differentiation of HepaRG liver progenitor cells" docx
... hybridization and data mining Pipeline profiling of transcriptional and translational changes occurring during hepatocytic differentiation of HepaRG cells Figure for Pipeline for profiling of transcriptional ... CAAT/enhancer-binding protein alpha; FN1, fibronectin; HGF, hepatocyte growth factor; HLF, hepatic leukemia factor; IFN, interferon; IGFBP, insulin growth factor-binding protein; IL, interleukin; LTBP1, ... the induction of a TH1 immune response to the hepatocyte and protects the normal parenchyma against liver injury [32] Jak2 participates in transduction of interleukin (IL)6 signaling in case of...
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Báo cáo y học: "cGlobal analysis of microRNA target gene expression reveals the potential roles of microRNAs in maintaining tissue identity" ppsx
... findings strongly suggest that miRNAs play a most important role in driving tissue terminal differentiation and particularly in maintaining tissue identity rather than in determining or regulating ... These findings strongly suggest that miRNAs play a most important role in driving tissue terminal differentiation and maintaining tissue identity rather than in determining or regulating tissue-specific ... understanding the global role of miRNAs in maintaining lineage of differentiated tissues and cells has great impact on the studies of miRNAs in cancer genetics 16 Methods Datasets used in this...
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Roles of microtubules and microtubule regulators in collective invasive migration of drosophila border cells
... 2.3.3 Roles of Stathmin in border cells .55 2.3.3.1 Effects of stathminKO on microtubules 55 2.3.3.2 Effects of stathminKO on border cell migration 57 2.3.3.3 Effect of stathminKO ... and in addition, to find out the roles of individual microtubule regulators including the previously identified gene stathmin in border cell migration 23 Results 2.1 Microtubules in the border cells ... fluorescence protein (GFP) tagged Tubulin (tubulin-GFP) Tubulin-GFP showed similar results (Figure 2.3), indicating that both anti-Tubulin and anti-actylated Tubulin staining in fixed sample can...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... dissociating into single cells following incubation with Accutase for minutes, collecting cells by centrifugation at 1000 rpm for minutes and replating at a ratio of 1:6 3.6.2 Differentiation of ReN-VM cells ... passaging of the hESCs For transfection of cells in one well of a six-well plate, 10 µg of plasmids was complexed with 10 µl of Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ... spheres of about 50 µm were obtained within weeks In order to culture neural progenitor cells (NPCs) as single cells in a monolayer, the neural spheres were dissociated into individual cells with...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... target 226 lincRNAs expressed in mESCs, and the authors identified 26 lincRNAs that resulted in loss of Nanog and Oct4 expression upon lincRNA knockdown, indicating that lincRNAs maintain the pluripotent ... evidence suggesting functional roles of lncRNAs in the brain includes a computational analysis of in situ hybridization data from the Allen Brain Atlas, which identified 849 lncRNAs showing specific ... binding to multiple chromatin regulatory proteins, thereby controlling the ES cell state In this study by Guttman et al (2011), loss -of- function experiments were performed using short hairpin...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... database indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell line, ... chromatin binding Quantitative PCR of the ChIP DNA revealed REST occupancy at the region upstream of RMST (pRMST), confirming that REST binds upstream of RMST in the human neural stem cell line (Figure ... highly expressed in the brain tissues, including the whole brain, fetal brain, cerebellum and substantia nigra, while weakly expressed in the other somatic tissues All three isoforms of RMST were...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... protein-protein interactions with several heterogenous nuclear ribonucleoproteins, including hnRNPA2B1 These evidences suggest that the RMST complex is composed of the RNA-binding protein, hnRNPA2B1 ... with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic stem cells (hESCs), and human neuronal development, using hESCs as a ... SOX2 in sensory neurogenesis Cell Stem Cell 8, 538-551 157 Crossley, P.H., Minowada, G., MacArthur, C.A., and Martin, G.R (1996) Roles for FGF8 in the induction, initiation, and maintenance of...
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ROLES OF HUNTINGTIN ASSOCIATED PROTEIN 1 IN INSULIN SECRETING CELLS
... of HAP1 in insulin-secreting cells after knockdown of its expression in INS-1 cell line The findings of HAP1 role in INS-1 cell may shed light on the understanding of complicated signaling pathways ... technique in INS-1 cells (a insulin secreting cell line from rat insulinoma) In insulin secretion experiment, the knockdown cells secreted less insulin upon the stimulation by high concentrations of ... and insulin HAP1 is also expressed in INS-1 cell line, a commonly-used insulin-secreting cell line from rat insulinoma Western blotting further confirms that there are two HAP1 isoforms in INS-1...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25