Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot

... In vitro characterization of a plastid terminal oxidase (PTOX) Eve-Marie Josse, Jean-Pierre Alcaraz, Anne-Marie Laboure´and Marcel KuntzUniversite´J. Fourier and CNRS (Plastes et ... cloning of the Arabidopsis gene, IMMUTANS,hasidentiﬁed a new important plastid located enzyme [1,2].Inactivation of this gene ﬁrst indicated that it was a cofactorfor early steps in carotenoid ... Joe¨t,T.,Genty,B.,Josse,E.M.,Kuntz,M.,Cournac,L.&Peltier,G. (2002) Involvement of a plastid terminal oxidase in plasto-quinone oxidation as evidenced by expression of the Arabidopsisthaliana enzyme in tobacco. J. Biol....

Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

... stimulation effect of DDI- 1A containing a right-handed aglycon helix wasgreater than that of DDI-1B; (e) the enhancementeffects of DDI- 1A and DDI-1B on AAT, CAG andCA repeats are stronger than ... polynucleotide kinase.DNA polymerase assays A standard reaction (15 lL) contained 4 lm each of theprimer and template and 1 mm each of deoxynucleoside tri-phosphate, DNA polymerase and the corresponding ... slippagesynthesis of various repeat DNA strands [16,20,28].Analogs of NCSi-gb, the aminoglucose in a- glycosidiclinkage or the natural sugar N-methylfucosamine in b-glycosidic linkage to the backbone,...

Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc

... AnI19R5¢-AATTCATGAGGAGGTTTCTCTGTAACA-3¢;AnI19H5¢-AGCTTGTTACAG-AGAAACCTCCTCATG-3¢; AnI17R5¢-AATTCACGAGGAGGTTTCTCTGTACTA-3¢;AnI17H 5¢-AGCTTAGTACAGAGAAACCTCCTCGTG-3¢;AnI15R5¢-AATTCACAGGAGGTTTCTCT-GTCTA-3¢;AnI15H5¢-AGCTTAGACAGAGAAACCTCCTGTG-3¢. Each annealed ... Coralville, IA, USA)were annealed together to make recognition site variantscontaining 5¢-EcoRI and 3¢-HindIII sticky ends: AnI19R5¢-AATTCATGAGGAGGTTTCTCTGTAACA-3¢;AnI19H5¢-AGCTTGTTACAG-AGAAACCTCCTCATG-3¢; ... thecomplete inability of a saturating amount of DNA substrateto inhibit RNA splicing (Fig. 6B) and therefore it seemsreasonable to make the working hypothesis that the RNAand DNA binding sites are...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... TCATCA TCA TCA GGG CAC GAC ACA CAT CCC C; andreverse primer, GAT CGG TAC CTC ATT ACA GAGGAG GGA TAT GGG GAA C. The PCR reaction wasdone as described above. The ampliﬁed PCR product wasinserted ... tested for tyrosinase activity with a plate assay. In the assay plates, Trichoderma minimal med-ium [19] with 2% lactose as a carbon source, 1% potassiumphthalate as a buffering agent (pH 5.5), ... processing of the protein. The intracellular tyro-sinases from N. crassa [26–28] and Agaricus [28] andPycnoporus species [25] have an additional C -terminal domain that is proteolytically released...

Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-afﬁnity substrate for H+/peptide cotransporters pdf

... wascustom synthesized by Amersham International (Little Chal-font, UK). Dexamethasone, apotransferrin, Gly-Gln, Ala-Ala, Ala-Ala-Ala, Lys-Lys, d-aminolevulinic acid, cefadroxil,Gly, Pro-Ala, ... ± 1Ala-Ala 15 ± 1Pro-Ala 105 ± 3Lys-Lys 46 ± 2Ala-Asp 19 ± 1D-Phe-Ala 65 ± 2Ala-Ala-Ala 21 ± 1d-Aminolevulinic acid 78 ± 3Cefadroxil 15 ± 1Lys[Z(NO2)]-Val 10 ± 18-Aminooctanoic acid ... synthesize a dipep-tide where l-4,4¢-biphenylalanine (Bip), with its largearomatic side chain in a short intramolecular distancefrom the a- carbon atom, is the N -terminal amino acidand l-Proline...

Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... theratio of protein concentration to ﬂavin concentration(as determined at 460 nm). blast and tfasta analysis of the phCoADR revealed a signiﬁcant level of identityto putative NADH oxidases ... demonstrate that this enzyme is notlikely to act as an NADH oxidase in vivo, instead act-ing as a CoADR. This is only the second demonstra-ted CoA reductase activity, and the ﬁrst appearance of this ... was removed and added to 900 lL of assay buffercontaining (ﬁnal concentrations) 5 mm DTNB, 0.25 mpotassium phosphate, 0.5 mm EDTA pH 7.2. After a 5-minincubation the absorbance was read at...

Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx

... l-aminoacids, including l-aspartate, l-glutamine, l-alanine,l-arginine, l-cysteine, l-proline, l-phenylalanine,l-lysine, l-tryptophan, l-isoleucine, l-tyrosine, l-histi-dine, l-leucine, l-valine, ... effect of several salts, metals and inhibitors on theinitial activity of Pf-TDH was checked using butane-2,3-diol as substrate in the standard oxidation reactionand acetoin in the reduction reaction. ... (5¢-GCGCGCCATGGCATCCGAGAAGATGGTTGCTATCA, sense)and BG1297 (5¢-GCGCGGGATCCTCATTTAAGCATGAAAACAACTTTGCC, antisense), containing NcoI andBamHI sites (underlined in the sequences). In order tointroduce an NcoI restriction...

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... l-amino acids such as amino acidoxidases, transaminases, and racemases (epimerases).For example, in pea seedlings the occurrence of d-amino acid aminotransferase was demonstrated [36].For a ... of the b-familycatalyse mainly b-replacement or b-elimination reac-tions. The d-alanine aminotransferase and the alanineracemase family are the other two independent lineages[6]. The b-family ... elimination and replacement reactions of amino acids have been puriﬁed and characterized [6].Keywords1-aminocyclopropane-1-carboxylatedeaminase; Arabidopsis thaliana;D-cysteine;desulfhydrase,...

Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt

... anti-gens.DiagnosisAspf1D(7–22)vs. Aspf1 a a-sarcinvs. Aspf1 a a-sarcinD(7–22)vs. Aspf1 a a-sarcinD(7–22)vs. a- sarcin a Asthma 33 33 50 20Cystic ﬁbrosis 50 30 89 33ABPA 20 33 70 22 a Data calculated ... activity of Aspf1 andits deletion mutant was also studied in a zymogramassay using poly (A) absorbed in a polyacrylamide gel(Fig. 6C). This assay showed the absence of any othercontaminating ... were: Nt-Aspf1 (5¢-GTCGTCTTGCGGTCACCTGGACATGCATCAACGAACAG-3¢) and Ct-Aspf1 (5¢-GTCGTCTTGGATCCTCTCGAGTCTCAATGAGAACACAGTCTCAAGTC-3¢). These primers contained BstEII andBamHI sites and were...

Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the speciﬁc and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf

... Seiichiro Ikeda1, Shinya Kajita1, Masaya Nakamura2and Yoshihiro Katayama11Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, ... notattack b-aryl ether linkages in high-molecular mass mate-rials in vivo because it acts at the intramolecular level andcannot gain access to the b-aryl ether linkages in high-molecular mass ... with bubbling18O2gas for 5 min(Nippon Sanso Co., Kawasaki, Japan) or18O-labeled water(94%18O atom; Nippon Sanso Co., Kawasaki, Japan) andincubatedfor12hat28°C. After the incubation, themixture...

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