Báo cáo khoa học: Molecular identiﬁcation of adrenal inner zone antigen as a heme-binding protein potx

... Min et al. Molecular properties of adrenal inner zone antigen FEBS Journal 272 (2005) 5832–5843 ª 2005 FEBS 5843 Molecular identiﬁcation of adrenal inner zone antigen as a heme-binding protein Li ... speciﬁcally with a monoclonal antibody raised against the fasciculata and reticularis zones of the rat adrenal, was previously found to be identical with a protein vari-ously named 25-Dx and membrane-associated ... in rattissue, ‘rIZA1’. The monoclonal antibody wascapable of inhibiting dose-dependently adrenal 21-hydroxylation of progesterone and 18-hydroxylationKeywords adrenal inner zone antigen; heme-binding protein; ...

Báo cáo khoa học: Molecular identiﬁcation of monomeric aspartate racemase from Biﬁdobacterium biﬁdum pptx

... 14252, aspartate racemase, which cata-lyzes the r ac emization of D-andL-aspartate, was puriﬁed tohomogeneity and characterized. T he enzyme was a mono-mer with a molecular mass of 27 kDa. ... containD-aspartate as the essential component [5]. Two kinds of amino acidracemases, alanine racemase [5] and glutamate racemase [6],have been identiﬁed ubiquitously from bacteria, a nd it hasbeen ... fD-glutamate.Most bacterial alanine racemases assemble in a dimerstructure [5], w hereas glutamate r acemases are m ainlycharacterized as monome ric enzymes [6]. On the otherhand, aspartate racemase...

Báo cáo khoa học: Molecular identiﬁcation and expression study of differentially regulated genes in the Paciﬁc oyster Crassostrea gigas in response to pesticide exposure doc

... 5¢-GAGCTCAGCGAGGACGGAAACCTCGCGT-3¢Lysosomalassociated protein ADI (up) 5¢-CCAATCAGGTAGGCCTTCATGGAGAGGA-3¢ 5¢-CCCAGAGATCCTCCAAGAGACAGCCAGT-3¢Elongation factor2 ADI (up) 5¢-ATCTGGAGAGCACATCATTGCTGGTGCA-3¢ 5¢-CTTTCTGGCCTCTCCAACATCCATGCCA-3¢Apolipophorin ... 5¢-GCACAGTTCCCCTACAGTCCCGCTTTAG-3¢HSCR ADI and G (up) 5¢-CCTTTTTAGCAGTGACTTTCCGTTGCAA-3¢ 5¢-CACGTCTCAGCAGGGAGAATAATCCCGA-3¢Senescenteassociated protADI (up) 5¢-TTGCAACGACTGCAGTCATCAGTAGGGT-3¢ 5¢-GAGCTCAGCGAGGACGGAAACCTCGCGT-3¢Lysosomalassociated protein ADI ... 5¢-TCTCCTACGATCATCTCACCGTCACCGA-3¢Guanyl cyclase ADI (down) 5¢-GGTTGTCAATTTATTGAATGATCTCTACA-3 5¢-CCTCGGATCTTGAGACCCTCGACTGGA-3¢ADP ribo G (down) 5¢-CATTTTGACTCGTCCCGATAAACAGGCA-3¢ 5¢-GTTTTACCGGCAGCATCCAACCCCACCA-3¢RiboL13...

Tài liệu Báo cáo khoa học: Direct identiﬁcation of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS docx

... formation, has a mass of 7743 Da.T. longibrachiatumThe main mass peak of T. longibrachiatum of m ⁄ z 7242was assigned to an HFB3-type of hydrophobin identiﬁedby searching the TrichoEST database. ... Calibration was done with the H. jeco-rina HFB1 protein with the 7533 Da calculated averageatomic mass. All masses determined correspond to averageatomic masses.MS analysis of low -molecular- mass peptidesMeasurements ... mass calculator(http://rna.rega.kuleuven.ac.be/masspec/pepcalc.htm). Tocorrelate observed mass peak data, masses of variousN- and C-terminally processed and oxidized peptides werecalculated...

Báo cáo khoa học: "Distributional Identiﬁcation of Non-Referential Pronouns" pdf

... approach are labelled as DISTRIB.Note that our maximum entropy classiﬁer actuallyproduces a probability of non-referentiality, whichis thresholded at 50% to make a classiﬁcation. As a baseline, ... rather part of a globally-optimal conﬁguration, as was done for general nounphrase anaphoricity by Denis and Baldridge (2007).The suitability of this kind of approach to correct-ing some of ... inter-annotator agreement could be calculated. A 1and A 2agreed on 96% of annotation decisions, while A 1 -A 3, and A 2 -A 3, agreed on 91% and 93% of de-cisions, respectively. The Kappa statistic...

Báo cáo khoa học: The identiﬁcation of a phospholipase B precursor in human neutrophils doc

... Possibleprotease contamination of the protein preparations wasruled out by the absence of protease activity when thecommercially available universal protease substrate,casein (resoruﬁn-labelled), was ... 42 kDa, there appeared minorbands at molecular masses of around 20 and 39–41 kDa, which partly shifted from the major bands,coinciding with the appearance of a signiﬁcant deacyla-tion activity. ... protease activity. It did not show anyprotease activity when the universal protease substratecasein–resoruﬁn was used as substrate and incubatedfor > 2 h at 37 °C. However after a few days at...

Báo cáo khoa học: Proteomic identiﬁcation of all plastid-speciﬁc ribosomal proteins in higher plant chloroplast 30S ribosomal subunit PSRP-2 (U1A-type domains), PSRP-3a/b (ycf65 homologue) and PSRP-4 (Thx homologue) doc

... 5¢-GGAATTCTAGATATCGTCGACAATTTGTGTTACTACCAAAATC-3¢;P2-4,5¢-GGAATTCGTCGACGCGTTAAAAAAGATAGCAGCATTGACAC-3¢;P3-1,5¢-ATGGGIAAYGARGTIGAYATHG-3¢; P3-2, 5¢-CTAGACCTATGTTTTTCTCCATCC-3¢; P4-1, 5¢-CCIAARAAYAARAAYAARGG-3¢; P4-2, 5¢-CAGATAGGAAGAGGGGCAAGGA-3¢;P4-3,5¢-GGAATTCTAGATATCGTCGACTTATCTTCAGAACTTGTTGC-3¢; ... 5¢-CAGATAGGAAGAGGGGCAAGGA-3¢;P4-3,5¢-GGAATTCTAGATATCGTCGACTTATCTTCAGAACTTGTTGC-3¢; P4-4, 5¢-GGAATTCGTCGACGCGTTTTTCAACAAATCATCATAT A- 3¢;TAG1,5¢-GGAATTCTAGATATCGTCG-3¢;TAG2, 5¢-GGAATTCGTCGACGCG-3¢.Computer ... 5¢-CGGGATCCGGTGGCGACGACTCCTGGAGCCC-3¢;PR,5¢-CGGGATCCCAACTGGTAATGGTAGCGACCGGC-3¢;P2-1, 5¢-ATGGAYATHGCIACIACICARGC-3¢;P2-2,5¢-TAGCAACTCATTCGTCACTGTC-3¢; P2-3, 5¢-GGAATTCTAGATATCGTCGACAATTTGTGTTACTACCAAAATC-3¢;P2-4,5¢-GGAATTCGTCGACGCGTTAAAAAAGATAGCAGCATTGACAC-3¢;P3-1,5¢-ATGGGIAAYGARGTIGAYATHG-3¢;...

Báo cáo khoa học: Structural identiﬁcation of ladderane and other membrane lipids of planctomycetes capable of anaerobic ammonium oxidation (anammox) pptx

... anammox bacteria, Candidatus ‘Scalindua brodae’and ‘Scalindua wagneri’, identiﬁed in a wastewatertreatment plant treating landﬁll leachate [8].Anammox catabolism takes place in a separatemembrane-bounded ... fromnature’, capable of anaerobic ammonium oxidation(anammox), was reported [1]. Based on 16S rRNAgene phylogeny, Candidatus ‘Brocadia anammoxidans’and its relative Candidatus ‘Kuenenia stuttgartiensis’were ... afteradding an internal standard (6,6-d2)3-methyleicosane), sil-ylated with BSTFA and analysed by GC. An internalstandard was added to an aliquot of the acetone-fractionand analysed by GC and...

Báo cáo khoa học: "Language Identiﬁcation of Search Engine Queries" pdf

... by automat-ically generating a data set of queries with lan-guage annotations. We show that the data gener-ated this way is highly reliable and can be used totrain a machine learning algorithm.We ... weassume that it is a fairly trivial task to identify thelanguage for an average web page using one of theexisting methods.3In our case, this task gets al-ready accomplished by the crawler ... search engines, and built a secondclassiﬁer that takes into account the geographicalinformation of the users.Human annotations on 5000 automatically an-notated queries showed that our data...

Báo cáo khoa học: The hyperﬂuidization of mammalian cell membranes acts as a signal to initiate the heat shock protein response pptx

... activation of kinases such as Akt has been shown to increaseHSF1 activity. Enhanced Ras maturation by heat stresswas associated with a heightened activation of extra-cellular signal-regulated kinase (ERK), ... cells, analogously with heat-inducedplasma membrane ﬂuidization, are indeed capable of activating HSP formation even at the growth tempera-ture, without causing measurable protein denaturation.We ... such a typical scenario, the activa-tion of growth factor receptors may in turn activatethe Ras ⁄ Rac1 pathway, which has been shown to play a critical role in HSF1 activation and HSP up-regula-tion...

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