in vitro target validation of il 12p40

In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles pptx

... results in the production of multiple inflammatory cytokines, including IL- 1, INF-γ and TNF-α production, which is known to cause tissue necrosis and organ failure In addition to inducing the ... production of pro-inflammatory cytokines, LPS also triggers the release of anti-inflammatory cytokines, such as IL- 10, in the bloodstream of normal mice [17] Many cell types can produce IL- 10, including ... with IL- 10 shRNA and the effects of cell apoptosis were determined by 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI) staining (C) Cell viability was determined by trypan blue staining...

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Báo cáo y học: " Efficient delivery of small interfering RNA for inhibition of IL-12p40 expression in viv" pdf

Danh mục: Báo cáo khoa học

... Silencing IL- 12p40 in vitro did not result in non-specific silencing of IL1 2p35, IL- 23p19, IL- 10, TNF-α, IL- 6 or IFN-γ mRNA However, silencing of IL- 12p40 by siRNA did result in a reduction of ... extended in vivo by showing that silencing of IL- 12p40 results in the nonpermanent suppression of IL- 12 in a murine model of peritoneal inflammation Such silencing is evident in the reduced levels of ... of IL- 12p70 was transient, with levels recovering at the remaining time points mRNA expression profiling for the inflammatory cytokines IFN-β, IL- 12p35, IL- 23p19, IL- 6, IL- 10 and IFNγ in IL- 12p40...

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Báo cáo y học: "In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains"

Danh mục: Y học thưởng thức

... Discussion We investigated the in vitro antimicrobial activities of the three kinds of Rice-fluid against various species of microorganisms including H pylori strains by the timekill-curve assay ... determined by using an in vitro time killing assay [14, 16] A bacterial suspension of 16 H pylori strains (100μl, approximately 108CFU/ml) was inoculated into 1ml of a Rice-fluid, a ten-folddilution ... phenomena were observed against other four strains of E coli, five strains of S aureus, S epidermidis, E faecalis and P aeruginosa, and three strains of K pneumoniae and two strains of K oxytoca, respectively...

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Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf

Danh mục: Báo cáo khoa học

... shown in Fig The packaging system contains the self-inactivating transducing constructs (sin) obtained Fig Diagram of lentiviral vector carrying the human IDUA cDNA Gene transfer vector includes ... containing 10% fetal bovine serum (Sigma), 100 UÆmL)1 penicillin, 100 mgÆmL)1 streptomycin and mM L-glutamine Cells were cultured at 37 °C in a 5% CO2 humidiﬁed incubator Lentiviral vector containing ... conﬂuency, and transduced with increasing doses of IDUA vector (1, 10 and 50 ng of p24 viral protein) in the absence or in the presence of polybrene (5 lgÆmL)1) After a 24-h incubation at 37 °C, the...

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Báo cáo khoa học: The in vitro nuclear aggregates of polyamines pot

Danh mục: Báo cáo khoa học

... ivNAPs in vitro model in order to investigate some of the features of the polyamine–phosphate interactions Speciﬁcally, we examined the role played by each polyamine in the self-assembly of in vitro ... weakness of the interactions involved In uence of NaCl on ivNAP stability In vitro NAPs were separated by GPC in the presence or absence of 150 mm NaCl in 50 mm phosphate buffer (pH 7.2) as mobile ... by NaCl in conferring stability on these supramolecular aggregates is a rough indication of the degree of difference in complexity between the in vitro and in vivo nuclear settings For instance,...

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báo cáo hóa học: "Measuring the impact of health problems among adults with limited mobility in Thailand: further validation of the Perceived Impact of Problem Profile" docx

Danh mục: Hóa học - Dầu khí

... pre-tested during training Clarity was confirmed through back-translation prior to pilot testing and finalizing the instrument The initial Thai language version of the PIPP was pilot tested in the study ... population of 19 million, is the largest of four regions in Thailand The majority of the population are of Lao descent and ethnicity, and those living in rural areas are among the poorest in Thailand ... resided in the poor northeast region, followed by the north of Thailand [14] A second survey conducted in 1999 produced similar findings [15] Studies have been undertaken in Thailand examining health...

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báo cáo hóa học:" The effect of abductor muscle and anterior-posterior hip contact load simulation on the in-vitro primary stability of a cementless hip stem" ppt

Danh mục: Hóa học - Dầu khí

... Loading Strain 2004, 40(1):193-202 43 Monti L, Cristofolini L, Toni A, Ceroni RG: In vitro testing of the primary stability of the VerSys enhanced taper stem: a comparative study in intact and intraoperatively ... motion In- vitro tests could at best simulate resorption by milling the bone interface at a predetermined location prior to testing [46] Nonetheless, the objective of in- vitro primary stability ... Description of application J Biomech 1972, 5(3):283-93 37 Cristofolini L, Vinteconti M: Towards the standardization of in vitro load transfer investigations of hip prostheses Journal of Strain Analysis...

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báo cáo hóa học:" Measuring the impact of health problems among adults with limited mobility in Thailand: further validation of the Perceived Impact of Problem Profile" doc

Danh mục: Hóa học - Dầu khí

... pre-tested during training Clarity was confirmed through back-translation prior to pilot testing and finalizing the instrument The initial Thai language version of the PIPP was pilot tested in the study ... population of 19 million, is the largest of four regions in Thailand The majority of the population are of Lao descent and ethnicity, and those living in rural areas are among the poorest in Thailand ... resided in the poor northeast region, followed by the north of Thailand [14] A second survey conducted in 1999 produced similar findings [15] Studies have been undertaken in Thailand examining health...

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Báo cáo vật lý: "Evaluation of In Vitro Antioxidant Activity of 5H-dibenz[b,f]azepine and Its Analogues" ppt

Danh mục: Báo cáo khoa học

... was performed in the absence of the compound to determine the amount of lipid peroxidation obtained in the presence of inducing agents as a control experiment The percentage of antilipid peroxidative ... by the coupling of o-nitro toluene (2 mM) in methanol in the presence of the catalyst ethyl formate and KOH (1 mM) in methanol by refluxing for hr to form bibenzyl (o,o'dinitroazepine) This was ... preparation of 10-methoxy-5H-dibenz[b,f]azepine (Compound d) 10-methoxy-5H-dibenz[b,f]azepine was prepared by brominating N-acetyl-5H-dibenz[b,f]azepine (2.35 g, 10 mM) using bromine (3.2 g, 20 mM) in...

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Báo cáo khoa học: "Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes" docx

Danh mục: Báo cáo khoa học

... phases However, Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes 69 Ta ble In vitro maturation, fertilization and cleavage rates of bovine oocytes relative to ... levels in the circulation resulting in the regression of dominant follicle and emergence of constant follicle wave turnover [21] The reasons for poor in vitro developmental competence of oocytes ... in vitro in the absence of serum and somatic cells: amino acids, vitamins and culturing embryos in groups stimulate development Biol Reprod 1994, 50,390-400 Gordon, I and Lu, K H Production of...

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Báo cáo lâm nghiệp: "In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker" ppsx

Danh mục: Báo cáo khoa học

... proliferating the axillary shoot within 2– weeks of culture Out of the various cytokinins tested, BAP was the most effective for inducing bud break and growth in axillary meristems Kinetin alone or in ... or in combination for induction of rooting Of the three auxins tested, IBA and IAA induced rooting (Tab IV) The optimum concentration was 0.5 mg/L of IBA or IAA and it resulted in 65 to 70% of ... was presented in Table III The interaction of two cytokinins with low concentration of auxin enhancing shoot multiplication has been reported by many authors [3, 5, 12] The inclusion of higher concentration...

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Báo cáo y học: "Immunohistological assessment of the synovial tissue in small joints in rheumatoid arthritis: validation of a minimally invasive ultrasound-guided synovial biopsy procedure" potx

Danh mục: Báo cáo khoa học

... series, considering one cutting level in five out of nine cases, two cutting levels in eight out of nine cases, and three cutting levels in all cases produced a reliable result cal review of the manuscript ... feasible through the examination of multiple specimens obtained by US-guided biopsy of small joints Microphotograph of an ultrasound-guided sample H & E staining of Microphotograph of an ultrasound-guided ... considering T-cell infiltration, lining layer thickness or vascularity scores [15] Our analysis focusing on synovial tissue obtained surgically from small joints reproduces these findings, demonstrating...

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Danh mục: Báo cáo khoa học

... Aggregates were incubated with 50 μL of the double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, ... repair of full-thickness articular cartilage defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained ... U/mL in distilled water), or with 0.25% trypsin containing mM EDTA for 15 minutes at 37°C for COL X detection, before sections were incubated overnight at 4°C primary antibodies diluted in 0.5%...

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báo cáo khoa học: " Uncovering high rates of unsafe injection equipment reuse in rural Cameroon: validation of a survey instrument that probes for specific misconceptions" ppsx

Danh mục: Báo cáo khoa học

... Another WHO target for improving injection safety in developing countries is eliminating unnecessary medical Page of injections The overuse of injectable medicines in developing countries is often ... strain on the profession [63] An ethos of triage has been invoked in the field of HIV research in the interests of shutting down debate over blood-borne HIV [31] A timely interest in pursuing ... volume between insertion of a needle and injection of the contents of the syringe shows that this difference offsets the reduction in inoculum volume achieved by rinsing a needle and syringe between...

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Báo cáo y học: " In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus" pdf

Danh mục: Báo cáo khoa học

... of Dimethyl sulfoxide (DMSO) ensuring stock concentration of 50 μg/μl Sieving the above solution by using 0.22 um filter inside Laminar Flow Hood, storing at -20°C Cell line The Huh-7 cell line ... plasmids containing HCV NS3 protease in the presence and absence of SN 100 μg extract and interferon by using Lipofectamine™ 2000 (Invitrogen life technologies, Carlsbad, CA) according to the manufacturer’s ... necessitating effective treatment Currently, there is no vaccine available for prevention of HCV infection due to high degree of strain variation The current treatment of care, Pegylated interferon a in...

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Báo cáo y học: "The triple combination of tenofovir, emtricitabine and efavirenz shows synergistic anti-HIV-1 activity in vitro: a mechanism of action study" pdf

Danh mục: Báo cáo khoa học

... Merrill DP, Videler JA, Chou TC, Byington RE, Eron JJ, D'Aquila RT, Hirsch MS: Two-drug combinations of zidovudine, didanosine, and recombinant interferon-alpha A inhibit replication of zidovudine-resistant ... NRTI+NRTI combinations was first ignited during the HIV monotherapy era by the surprisingly synergistic effects of AZT+ddI both in vitro and in clinical trial studies [22-24], in the absence of a pharmacokinetic ... effect of TFV+FTC combination, and the statistically significant increases in the levels of the active metabolites in T-cell line CEM[30] To investigate whether the synergistic effect of TFV+FTC in...

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Báo cáo y học: "In vitro nuclear interactome of the HIV-1 Tat protein" pps

Danh mục: Báo cáo khoa học

... we included interactions involving a minimum of two proteins Non-interacting and self-interacting proteins were excluded to simplify the network representation The resulting network consists of ... Figure Tat Validation of the identity of selected proteins interacting with Validation of the identity of selected proteins interacting with Tat A GST pull-downs were performed with immobilised GST ... composed of lamins and NE lamin binding proteins including Nesprin1 alpha, MAN1, lamina-associated polypeptides-1 and (LAP1, LAP2), emerin and Lamin B receptor[254,255] The four latter and Lamin B...

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Báo cáo sinh học: "In vivo gene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery" pot

Danh mục: Báo cáo khoa học

... diseases, including infectious disorders, cancer and failure of the hematopoietic system [1,2] In vivo gene transfection is more appealing than in vitro transfection of an aliquot of cells or ... and is readily internalised upon binding to its ligand [17] Thus, targeted-gene transfer through CD117 may be achieved in this cell subset We indeed demonstrated an in vivo targeting of hematopoietic ... with mM of oligo-dT15 (SigmaAldrich) and then incubated at 42°C for hour in the presence of 100 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen) in a final volume of 20...

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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Danh mục: Cao đẳng - Đại học

... and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 ... crowding on protein stability Proteins (1):133-138 78 Harve KS, Lareu R, Rajagopalan R, et al 2010;Understanding how the crowded interior of cells stabilizes DNA/DNA and DNA/RNA hybrids -in silico ... Mariman E 2004;A combination of protein profiling and isotopomer analysis using matrixassisted laser desorption/ionizationtime of flight mass spectrometry reveals an active metabolism of the extracellular...

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Báo cáo y học: " Cytosolic group IVa phospholipase A2 mediates IL-8/CXCL8-induced transmigration of human polymorphonuclear leukocytes in vitro" ppsx

Danh mục: Báo cáo khoa học

... (a target protein of PI3K) using inhibitors of activated gIVaPLA2 Accordingly, these data indicate a downstream regulatory role for gIVaPLA2 in in vitro PMN migration subsequent to activation of ... enhanced chemiluminescence (Amersham, Arlington Heights, IL) Isolated PMNs were incubated in RPMI medium containing 5% FBS and 0.5 μCi [3H]AA Labeled PMNs were incubated for h and unincorporated ... upstream activation of signaling kinases in eliciting PMN migration Methods Antibodies and reagents IL- 8/CXCL8 was purchased from Peprotech (Rocky Hill, NJ) while bovine serum albumin fraction V and...

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