... 37°C in a microaerophilic atmosphere The remaining bacterial species were also determined in almost the same manner as inthe case of H pylori strains described above Heart Infusion agar plates ... on and/or enhanced under strengthened CO2 gas conditions On the other hand, the emergence of antibiotic resistant H pylori strains has been increasing and the problems for the chemotherapy have ... is rather narrow In fact, the bactericidal activities were expressed against only the restricted bacterial species sharing with the common properties that their growths are dependent on and/or...
... in real communication, which helps increase their interest in their learning In other words, their learning has concrete purposes which are future work-oriented II.2.3 Some problems of presentation ... every second or third name or other patterns instead of calling them in order otherwise they will know their turn and they not concentrate on their work as someone else but not them who is going ... purpose, content not form, variety of language, no teacher intervention or learner-centredness, and no material control Furthermore, the examples of these techniques are diverse including roleplay,...
... and nuclear transport machinery The Nuclear Pore Complex (NPC) located inthe nuclear envelope (NE) is composed ofthe outer nuclear membrane (ONM) and the inner nuclear membrane (INM), and enables ... interaction domains of RNA (RRM, DEXDc, DSRM) and DNA (HMG and SANT) recognition motifs [50-53] Other enriched interaction domains are well known to be involved in mediating protein-protein interactions ... nuclear lamina lines the INM and is composed of lamins and NE lamin binding proteins including Nesprin1 alpha, MAN1, lamina-associated polypeptides-1 and (LAP1, LAP2), emerin and Lamin B receptor[254,255]...
... required a minimum of 239 nt from the 3¢-end Furthermore, we have reported that an oligonucleotide complementary to the SL-B1 domain inthe 3¢-end ofthe HCV minus-strand RNA inhibits invitro initiation ... the RNA fragment corresponding to the 3¢-end ofthe HCV minus-strand RNA did not significantly alter the initiation site of RNA synthesis by recombinant HCV NS5B from different viral strains Analysis ... several times To analyze the RNA synthesized in one round of synthesis, we performed RdRp assays inthe presence of heparin in order to prevent reinitiation In a first step we determined the heparin...
... and Robert Morell and Yandan Yang for helping in DNA sequencing This research was supported by the Intramural Research Program ofthe NIH, NIDCD The analytical work was supported in part by the ... lL of 106 colony forming units, CFU) were inoculated into 190 lL reaction wells containing the diluted normal human serum, 25% of heat-inactivated normal human serum, or DPBSG alone and incubated ... catarrhalis outer membrane [19–24] LOS role inM catarrhalis outer membrane M catarrhalis LOS is a major component ofthe bacterial outer membrane with three main serotypes A, B and C, of which...
... hypoglycemia lead to increased GLUT1 protein synthesis due to negative regulation ofthe RNA binding proteins hnRNP A2 and hnRNP L, which bind an AU-rich response element inthe GLUT1 ⁄ UTR under normoxic ... is the main supplier of redox equivalents for the respiratory chain, a reduction of its turnover rate lowers the mitochondrial transmembrane potential, the formation rate of ROS and the rate of ... in normal canine mammary gland and in mammary tumours and their correlation with oestrogen receptors J Comp Pathol 102, 191–195 220 Losman MJ, Rimon D, Kim M & Becker MA (1985) Selective expression...
... measured by monitoring the change inthe concentration of p-nitroaniline, because p-nitroaniline is not released from the substrate when the peptide bond N- terminal ofthe proline residue is inthe cis ... °CÆmin)1 Prior to the measurements, samples were filtered through 0.22 lm pore size membranes and then degassed in a vacuum The protein concentrations during the measurements were 0.5 mgÆmL)1 The reversibility ... The initial loading concentration ofthe protein was 1.8 mgÆmL)1 The protein concentration distribution within the cell was monitored by the absorbance at 280 nm Analysis ofthe sedimentation...
... that an indirect in uence exists, possibly via the mediation of structural modifications induced by redox partner binding or by the interaction with other proteins ofthe mitochondrial membrane, ... chains ofthe corresponding residues in each ofthe protein templates; (d) searching for protein loops in order to design the backbone conformations ofthe structurally variable regions (SVRs) ... enzymatic activity The same observation was made for the single mutant K15 2N (M Hampf, Max-Delbruck¨ Centre, Berlin, Germany, personal communication) In contrast, all other mutants induced markedly...
... nm, missing in single polyamine solutions (data not shown), is the demonstration that the aggregation ofthe single components determines an impressive change in their electronic properties The ... molecular complexes Another intriguing point is the relationship existing between the three-dimensional arrangement of these structures and the regular production of only three main compounds, whatever ... since this theory predicts the production of only oligomers and a preferential disposition ofthe unimers in a linear chain, rather than their columnar stacking, if the hydrogen bonds are single...
... been shown to have the most pronounced effect on femoral strains, increasing medial bending inthe proximal femur during gait [26-28] There are, however, contradictory results concerning the ... a single axis motion measurement approach, however, rotational motions between the implant and the bone can incur large errors in translational motion measurement, which are proportional to the ... 3Department of Materials Engineering, University of British Columbia, Vancouver, Canada, 4Department of Mechanical and Aerospace Engineering, Carleton University, Ottawa, Canada and 5Department of...
... increasing concentrations The induction of β-galactosidase was determined by OD420nm 3) The estrogenicity inthe combination of two weak estrogens The combinations of some concentrations of nonlyphenol ... recombinant assay Saccharomyces cerevisiae ER+ LYS 8127 was grown for 18 h inthe presence of nonylphenol or butylparaben alone and in combinations at increasing concentrations The induction of ... some combinations of some low concentrations of estrogenic compounds produced greater effect than the sum ofthe parts in this recombinant yeast assay, synergistic interactions ofthe binary mixture...
... Weber M, Steinert A, Jork A, Dimmler A, Thurmer F, Schutze N, Hendrich C, Zimmerman U: Formation of cartilage matrix proteins by BMP-transfected murine mesenchymal stem cells encapsulated in a novel ... limited by its invitro nature Therefore, clarifying in vivo experiments are necessary before such factors can be recommended for further clinical use Conclusions Adenoviral BMP-4 gene transfer ... and internal control For each marrow preparation/patient, treatment group and time point indicated RNA was extracted from 10 aggregates, and four patients were analyzed depending on group and...
... trapping the enzyme in a polymerase-independent RNase H-competent mode of binding [58] Our study demonstrated that part ofthe NNRTI inhibition may also come from the trapping of enzyme once the primer ... determined by three kinetic constants including the dissociation constant (Kd), the maximum percentage of DNA primer-template forming a tight binding RT-DNA complex (Bmax), and the ratio of Bmax/Kd ... pattern of binding orientations compared to a traditional DEC bound with dNTP where only the polymerase-competent binding orientation is observed In either binding orientation, the EFV-based complex...
... a certain sense and reference, one is normally doing something other than just saying something like making a request or making a promise or an offer, etc According to Austin, there are three ... described in terms ofthe level of their psychological consequences, often performing an act by saying something such as persuading, convincing, scaring, enlightening, inspiring, or otherwise ... way of relying on their mutually shared background information, both linguistic and nonlinguistic, together with the general powers of rationality and inference on the part ofthe hearer.” Indirectness...
... intention via the process of inferences Therefore, the hearer needs to take all the circumstances surrounding the conversation because they can make a lot of help in inferring the hidden intention People ... other strategies This seems to suggest that in trading interactions, people tend to show their attention, concerns or deference to others in order to maintain social harmony As mentioned inthe ... on record as incurring a debt or as not indebting a hearer Speaker can redress an FTA by explicitly claiming his indebtedness to hearer, or by disclaiming any indebtedness of hearer, by means...
... defines them like this: Locutionary act: performing an act of saying something; Illocutionary act: performing an act in saying something; Perlocutionary act: performing an act by saying something ... producing a further effect on the H As for his definition, perlocutionary often performs an act by saying something such as persuading, convincing, scaring, enlightening or otherwise getting someone ... Desolate S, H from the particular infringement State the FTA as a general rule Nominalize 10 Go on record as incurring a debt, or as not indebting H Redress other wants of H’s derivative from negative...