in vitro pharmacological effects of phosphorothioate oligodeoxynucleotides

... http://www.cmjournal.org/content/4/1/14 Inhibition rate % Chinese Medicine 2009, 4:14 acetylshikonin μg/ml Figure cells (n = effects of acetylshikonin on the growth of tumor Inhibitory 3) Inhibitory effects of acetylshikonin on ... 72 Figure determined by cell acetylshikonin on (n growth of A549 Inhibitory effects ofgrowth curve assaythe = 3) Inhibitory effects of acetylshikonin on the growth of A549 determined by cell growth ... was obtained from the Cell Bank of the Chinese Academy of Sciences The cells were cultured in RPMI 1640 (Gibco, USA) supplemented with 10% fetal bovine serum (Minhai Bio-engineering, China) MTT...

Ngày tải lên: 13/08/2014, 15:21

... raw rice Discussion We investigated the in vitro antimicrobial activities of the three kinds of Rice-fluid against various species of microorganisms including H pylori strains by the timekill-curve ... phenomena were observed against other four strains of E coli, five strains of S aureus, S epidermidis, E faecalis and P aeruginosa, and three strains of K pneumoniae and two strains of K oxytoca, respectively ... consisting of three kinds of Rice-fluid derived from the two kinds of raw rice; i e., polished (pH6.32) and unpolished (pH5.98), and the usually cocked rice (pH5.91), and remaining one compound of...

Ngày tải lên: 31/10/2012, 17:03

... The Effects of Reductions in Family Planning Funds As noted earlier, the planned changes in the WHP follow on major reductions in funding for family planning services in Texas beginning in 2011.46 ... Operational restructuring, such as by closing some sites and reducing staff, in light of the loss of operating funds Reexamination of their services, perhaps reducing the use of long-acting reversible ... 20.http://www.statecoverage.org/files/RWJF_Cost_Sharing _Effects_ on_spending_and_outcomes.pdf 49 Ku, L & Wachino, V (2005) “The Effect Of Increased Cost-Sharing in Medicaid: A Summary Of Research Findings” Washington, DC: Center...

Ngày tải lên: 13/02/2014, 06:20

... accounting for 90% of the organic matrix, with non-collagenous proteins making up the remaining 10%, including glycoproteins (i.e alkaline phosphatase and osteonectin), Gla-containing proteins ... accurate measurement of serum BSP remains challenging Other non-collagenous proteins include osteopontin, which belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family like ... lining cells on quiescent bone surfaces Finally, PTH increases intestinal absorption of calcium by enhancement of 1,25 dihydroxyvitamin D synthesis [53, 54] The involvement of the skeleton in...

Ngày tải lên: 14/02/2014, 21:20

... 2004:54) The influence of Yin & Yang can also be seen in the naming accounting technique of both Longmen Zhang bookkeeping, which emerged at the end of the Ming and the beginning of the Qing dynasties; ... perspective more dominant or more effective on Chinese accounting environment Influenced by the increasing interest in cultural influence on accounting in international accounting research, some ... general insight about the factors influencing the evolution of Chinese accounting, during Chinese history (Gao and Schachler, 2004:54): Figure 3-Factors of Influencing the Evolution of Chinese...

Ngày tải lên: 17/02/2014, 10:20

... combinatorial realities of fine-grained sampling are daunting, because the number of trials increases proportionally to the square of the number of sample values along the dimension(s) of interest ... asymmetries in off-center compositions The genesis of this line of research appears to be an early claim by Wölf in (1928), as reported in Gaffron (1950), that aesthetically pleasing paintings generally ... of variance on the positions of the centers of the objects indicate a main effect of facing direction (F (1, 9) = 6.99, p < 0.03), but not of instructional condition, F (1, 9) < 1, or their interaction...

Ngày tải lên: 07/03/2014, 17:20

... stoichiometry of 0.9, and molar lipid/protein ratio of 1200 The intra- and interday degree of variation in relative P450, reductase, and lipid content did not exceed 10% in any of the vesicular ... activation of the CYP1A1-catalyzed oxidation of the (+)-7,8-diol was less pronounced Effects of nonbilayer lipids on the metabolic rates of epoxidation We analyzed the effects of a typical member of ... associating the protein partially buried into the membrane [35] This would favour lipophilic substrates by placing the opening of the substrate access channel into the lipid bilayer Thus the pool of...

Ngày tải lên: 08/03/2014, 10:20

... the interaction of glucokinase with binding proteins [5,6] The glucokinase regulatory protein (GKRP) is a specific inhibitor of glucokinase [5] and also a nuclear receptor that sequesters glucokinase ... glucokinase and hexokinase I in distinct pools [3,24], or by the involvement of mechanisms, additional to Glc6P, in mediating the effects of glucokinase overexpression As glucokinase binds to a dual-specificity ... the increase of Glc6P in the presence of S4048 was caused by the inhibition of Glc6P hydrolysis in the absence of detectable changes in glucose phosphorylation S4048 causes inactivation of phosphorylase...

Ngày tải lên: 16/03/2014, 14:20

... shown in Fig The packaging system contains the self-inactivating transducing constructs (sin) obtained Fig Diagram of lentiviral vector carrying the human IDUA cDNA Gene transfer vector includes ... MluI to obtain the self inactivating (sin) gene transfer construct PRRLsinPPT.CMV IDUA.WPRE The packaging of the vector was obtained as described previously [23,24] by cotransfection of 293T cells ... confluency, and transduced with increasing doses of IDUA vector (1, 10 and 50 ng of p24 viral protein) in the absence or in the presence of polybrene (5 lgÆmL)1) After a 24-h incubation at 37 °C, the...

Ngày tải lên: 24/03/2014, 04:21

... Considerations in Diaphyseal Modeling 112 The Mechanical Role of Remodeling 114 Gender Differences in Modeling during Puberty 114 Animal Studies of the Effects of Sex Hormones on Modeling 116 Male ... Preface xxiii Chapter I Epidemiology of Fractures L Joseph Melton 111 I Introduction 11 Effects of Age 111 Effects of Gender IV Effects of Race V Effects of Geography VI Secular Trends VII Public ... result of falls, and the risk of falling increases with aging in men as well as women (Winner et al., 1989) Consequently, there are age-related increases not only for hip and spine fractures in men...

Ngày tải lên: 28/03/2014, 23:20

... ivNAPs in vitro model in order to investigate some of the features of the polyamine–phosphate interactions Specifically, we examined the role played by each polyamine in the self-assembly of in vitro ... by NaCl in conferring stability on these supramolecular aggregates is a rough indication of the degree of difference in complexity between the in vitro and in vivo nuclear settings For instance, ... types of polyamine aggregate are more effective than single polyamines in the carrying out of the crucial functions of the dsDNA protection and conformation, thus indicating that polyamine aggregation...

Ngày tải lên: 29/03/2014, 23:20

... 5(3):283-93 37 Cristofolini L, Vinteconti M: Towards the standardization of in vitro load transfer investigations of hip prostheses Journal of Strain Analysis for Engineering Design 1999, 34(1):1-15 ... Measurement of the Relative Motion Between an Implant and Bone under Cyclic Loading Strain 2004, 40(1):193-202 43 Monti L, Cristofolini L, Toni A, Ceroni RG: In vitro testing of the primary stability of ... similar finding was reported in another study, in which stair climbing loads generated approximately 150 μm of distal migration, compared to 30 μm of proximal migration when simulating walking loads...

Ngày tải lên: 20/06/2014, 04:20

... cultivation intensity of maize or OSR in the neighbourhood of each of the 7,338 NSG in Germany The combination of GC and CC resulted in a total of nine risk categories [RC], describing the potential ... susceptibility/sensitivity of nature reserves [NSG] in Germany as being part of the receiving environment that might be affected due to GMP cropping in their vicinity Within the joint research project ... firstly, to analyse principles of GMO EM as published in the Association of German Engineers [VDI] Guideline 4330 Part 1, focusing on the characterisation of the receiving environment affected...

Ngày tải lên: 20/06/2014, 20:20

... cultivation intensity of maize or OSR in the neighbourhood of each of the 7,338 NSG in Germany The combination of GC and CC resulted in a total of nine risk categories [RC], describing the potential ... susceptibility/sensitivity of nature reserves [NSG] in Germany as being part of the receiving environment that might be affected due to GMP cropping in their vicinity Within the joint research project ... firstly, to analyse principles of GMO EM as published in the Association of German Engineers [VDI] Guideline 4330 Part 1, focusing on the characterisation of the receiving environment affected...

Ngày tải lên: 21/06/2014, 17:20

... experiment was performed in the absence of the compound to determine the amount of lipid peroxidation obtained in the presence of inducing agents as a control experiment The percentage of antilipid peroxidative ... preparation of 10-methoxy-5H-dibenz[b,f]azepine (Compound d) 10-methoxy-5H-dibenz[b,f]azepine was prepared by brominating N-acetyl-5H-dibenz[b,f]azepine (2.35 g, 10 mM) using bromine (3.2 g, 20 mM) in ... outlined in schemes 1–3 In scheme 3, to obtain compound (e), the reaction was carried out by using a weak base (triethyl amine) with COCl2 at room temperature (RT) instead of triphosgene in the...

Ngày tải lên: 07/08/2014, 14:20

... phases However, Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes 69 Ta ble In vitro maturation, fertilization and cleavage rates of bovine oocytes relative to ... levels in the circulation resulting in the regression of dominant follicle and emergence of constant follicle wave turnover [21] The reasons for poor in vitro developmental competence of oocytes ... during in vitro culture The present data are not sufficient to draw any significant conclusions However, it supports the previous findings that renewal of medium every 48 hours during in vitro...

Ngày tải lên: 07/08/2014, 17:22

... proliferating the axillary shoot within 2– weeks of culture Out of the various cytokinins tested, BAP was the most effective for inducing bud break and growth in axillary meristems Kinetin alone or in ... or in combination for induction of rooting Of the three auxins tested, IBA and IAA induced rooting (Tab IV) The optimum concentration was 0.5 mg/L of IBA or IAA and it resulted in 65 to 70% of ... shoot having 3–4 nodes) within weeks of subculture (Tab II) (Fig 1b) Analysis of variance for the effects of cytokinins and auxins on production of multiple shoots and average number of shoots/explant...

Ngày tải lên: 08/08/2014, 01:21

... [16,17], including the combinations: dicloxacillin/amikacin, cephalothin/amikacin, cephalothin/vancomycin, imipenem/vancomycin and vancomycin/amikacin Stock solutions were prepared according to ... MIC in combination for a = dicloxacillin plus amikacin, b = cephalothin plus amikacin, c = cephalothin plus vancomycin, d = imipenem plus vancomycin and e = vancomycin plus amikacin Page of (page ... as follows: FIC of drug A (FICA) = MIC of drug A in combination/MIC of drug A alone, and the FIC of drug B (FICB) = MIC of drug B in combination/MIC of drug B alone If the MIC of any agent alone...

Ngày tải lên: 08/08/2014, 19:20

... Aggregates were incubated with 50 μL of the double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, ... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... http://arthritis-research.com/content/11/5/R148 Our study is also in agreement with studies of in vitro chondrogenesis with primary MSCs using recombinant proteins, where BMP-4 was identified as a strong inducer of chondrogenesis [36],...

Ngày tải lên: 09/08/2014, 14:22

... plasmids containing HCV NS3 protease in the presence and absence of SN 100 μg extract and interferon by using Lipofectamine™ 2000 (Invitrogen life technologies, Carlsbad, CA) according to the manufacturer’s ... of Dimethyl sulfoxide (DMSO) ensuring stock concentration of 50 μg/μl Sieving the above solution by using 0.22 um filter inside Laminar Flow Hood, storing at -20°C Cell line The Huh-7 cell line ... there is no vaccine available for prevention of HCV infection due to high degree of strain variation The current treatment of care, Pegylated interferon a in combination with ribavirin is costly,...

Ngày tải lên: 11/08/2014, 21:21