prostheses in vitro flow dynamics of

... raw rice Discussion We investigated the in vitro antimicrobial activities of the three kinds of Rice-fluid against various species of microorganisms including H pylori strains by the timekill-curve ... phenomena were observed against other four strains of E coli, five strains of S aureus, S epidermidis, E faecalis and P aeruginosa, and three strains of K pneumoniae and two strains of K oxytoca, respectively ... consisting of three kinds of Rice-fluid derived from the two kinds of raw rice; i e., polished (pH6.32) and unpolished (pH5.98), and the usually cocked rice (pH5.91), and remaining one compound of...

Ngày tải lên: 31/10/2012, 17:03

... closeness to international capital markets Other SRDBs are introducing innovations in microfinance and SME financing with instruments such as loans to financial intermediaries, financial support ... between (i) improving the financial position of the institution by increasing reserves and strengthening lending capacity; (ii) providing grants, increasing concessional lending, or building fiduciary ... financial situation and capacity to mobilize financial resources, (iv) innovation in financial instruments and customization of their interventions, and (v) patterns of allocating net income in...

Ngày tải lên: 16/02/2014, 06:20

... shown in Fig The packaging system contains the self-inactivating transducing constructs (sin) obtained Fig Diagram of lentiviral vector carrying the human IDUA cDNA Gene transfer vector includes ... MluI to obtain the self inactivating (sin) gene transfer construct PRRLsinPPT.CMV IDUA.WPRE The packaging of the vector was obtained as described previously [23,24] by cotransfection of 293T cells ... confluency, and transduced with increasing doses of IDUA vector (1, 10 and 50 ng of p24 viral protein) in the absence or in the presence of polybrene (5 lgÆmL)1) After a 24-h incubation at 37 °C, the...

Ngày tải lên: 24/03/2014, 04:21

... ivNAPs in vitro model in order to investigate some of the features of the polyamine–phosphate interactions Specifically, we examined the role played by each polyamine in the self-assembly of in vitro ... by NaCl in conferring stability on these supramolecular aggregates is a rough indication of the degree of difference in complexity between the in vitro and in vivo nuclear settings For instance, ... types of polyamine aggregate are more effective than single polyamines in the carrying out of the crucial functions of the dsDNA protection and conformation, thus indicating that polyamine aggregation...

Ngày tải lên: 29/03/2014, 23:20

... 5(3):283-93 37 Cristofolini L, Vinteconti M: Towards the standardization of in vitro load transfer investigations of hip prostheses Journal of Strain Analysis for Engineering Design 1999, 34(1):1-15 ... Kilvington M: In vivo records of hip loads using a femorsl implant with telemetric output (a preliminary report) Journal of Biomedical Engineering 1979, 1:111-115 Rydell NW: Forces acting in the ... Measurement of the Relative Motion Between an Implant and Bone under Cyclic Loading Strain 2004, 40(1):193-202 43 Monti L, Cristofolini L, Toni A, Ceroni RG: In vitro testing of the primary stability of...

Ngày tải lên: 20/06/2014, 04:20

... experiment was performed in the absence of the compound to determine the amount of lipid peroxidation obtained in the presence of inducing agents as a control experiment The percentage of antilipid peroxidative ... preparation of 10-methoxy-5H-dibenz[b,f]azepine (Compound d) 10-methoxy-5H-dibenz[b,f]azepine was prepared by brominating N-acetyl-5H-dibenz[b,f]azepine (2.35 g, 10 mM) using bromine (3.2 g, 20 mM) in ... outlined in schemes 1–3 In scheme 3, to obtain compound (e), the reaction was carried out by using a weak base (triethyl amine) with COCl2 at room temperature (RT) instead of triphosgene in the...

Ngày tải lên: 07/08/2014, 14:20

... phases However, Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes 69 Ta ble In vitro maturation, fertilization and cleavage rates of bovine oocytes relative to ... levels in the circulation resulting in the regression of dominant follicle and emergence of constant follicle wave turnover [21] The reasons for poor in vitro developmental competence of oocytes ... during in vitro culture The present data are not sufficient to draw any significant conclusions However, it supports the previous findings that renewal of medium every 48 hours during in vitro...

Ngày tải lên: 07/08/2014, 17:22

... proliferating the axillary shoot within 2– weeks of culture Out of the various cytokinins tested, BAP was the most effective for inducing bud break and growth in axillary meristems Kinetin alone or in ... or in combination for induction of rooting Of the three auxins tested, IBA and IAA induced rooting (Tab IV) The optimum concentration was 0.5 mg/L of IBA or IAA and it resulted in 65 to 70% of ... was presented in Table III The interaction of two cytokinins with low concentration of auxin enhancing shoot multiplication has been reported by many authors [3, 5, 12] The inclusion of higher concentration...

Ngày tải lên: 08/08/2014, 01:21

... Aggregates were incubated with 50 μL of the double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, ... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... http://arthritis-research.com/content/11/5/R148 Our study is also in agreement with studies of in vitro chondrogenesis with primary MSCs using recombinant proteins, where BMP-4 was identified as a strong inducer of chondrogenesis [36],...

Ngày tải lên: 09/08/2014, 14:22

... of Dimethyl sulfoxide (DMSO) ensuring stock concentration of 50 μg/μl Sieving the above solution by using 0.22 um filter inside Laminar Flow Hood, storing at -20°C Cell line The Huh-7 cell line ... plasmids containing HCV NS3 protease in the presence and absence of SN 100 μg extract and interferon by using Lipofectamine™ 2000 (Invitrogen life technologies, Carlsbad, CA) according to the manufacturer’s ... there is no vaccine available for prevention of HCV infection due to high degree of strain variation The current treatment of care, Pegylated interferon a in combination with ribavirin is costly,...

Ngày tải lên: 11/08/2014, 21:21

... MS: Synergistic inhibition of replication of human immunodeficiency virus type 1, including that of a zidovudine-resistant isolate, by zidovudine and 2',3'-dideoxycytidine in vitro Antimicrob ... NRTI+NRTI combinations was first ignited during the HIV monotherapy era by the surprisingly synergistic effects of AZT+ddI both in vitro and in clinical trial studies [22-24], in the absence of a pharmacokinetic ... effect of TFV+FTC combination, and the statistically significant increases in the levels of the active metabolites in T-cell line CEM[30] To investigate whether the synergistic effect of TFV+FTC in...

Ngày tải lên: 12/08/2014, 23:21

... depicted in Figure 5[75233] Global network characteristics Within the interaction map we included interactions involving a minimum of two proteins Non-interacting and self-interacting proteins were ... composed of lamins and NE lamin binding proteins including Nesprin1 alpha, MAN1, lamina-associated polypeptides-1 and (LAP1, LAP2), emerin and Lamin B receptor[254,255] The four latter and Lamin B ... number of appearance and percentage of each of the motifs are shown N Dataset: Number of proteins in Tat interactome dataset possessing motif annotation % Dataset: percentage of proteins in Tat interactome...

Ngày tải lên: 12/08/2014, 23:21

... and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 ... decorin and fibromodulin with transforming growth factor beta Biochem J :527534 24 Iozzo RV 1999;The biology of the small leucine-rich proteoglycans Functional network of interactive proteins ... Z, Mo ZY, et al 2006;Mixed macromolecular crowding accelerates the refolding of rabbit muscle creatine kinase: implications for protein folding in physiological environments J Mol Biol (3):469-482...

Ngày tải lên: 11/09/2015, 09:16

... analysis and interpretation of data of erythrocyte aggregation of the in vitro experiments GP: acquisition, analysis and interpretation of data of erythrocyte deformability of the in vitro experiments ... chromatography Also involved in drafting the manuscript, revising it critically and in giving final approval of the version to be published LU: acquisition, analysis and interpretation of data of laboratory ... diminished in the HA group This suggests that incubation with HA introduces changes in the conformation of the cytoskeletal protein spectrin Table 1: Erythrocyte aggregability in the presence of...

Ngày tải lên: 10/08/2014, 05:21

... recombination breakpoints distribution Considering this, sequence analysis was performed taking into account if the breakpoint was located in the Vpr or in the Tat coding region from a total of ... factor binding sites present in the LTR revealed a high conservation of most of them Nevertheless, a remarkable high frequency of sequences harboring mutations in the NFkβ and Sp1 binding sites ... one comprising the viral promoter and part of the Gag coding sequence (positions 128 to 944 in the HXB2 numbering), and the other including the 3' half of Vpr and 5' half of Tat coding sequences...

Ngày tải lên: 12/08/2014, 04:21

... recombination breakpoints distribution Considering this, sequence analysis was performed taking into account if the breakpoint was located in the Vpr or in the Tat coding region from a total of ... factor binding sites present in the LTR revealed a high conservation of most of them Nevertheless, a remarkable high frequency of sequences harboring mutations in the NFkβ and Sp1 binding sites ... one comprising the viral promoter and part of the Gag coding sequence (positions 128 to 944 in the HXB2 numbering), and the other including the 3' half of Vpr and 5' half of Tat coding sequences...

Ngày tải lên: 12/08/2014, 04:22

... the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding is in concurrence ... statins including inhibition of the metabolism [5], administration of statins with certain juices [6] or inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase ... pravastatin However, the relative impacts of different in vitro findings on the overall in vivo drug delivery efficacy of these cellular carriers remain to be evaluated during the future in vivo...

Ngày tải lên: 25/10/2012, 11:10

... phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, ... containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All of the ... mini-gel columns of Sephadex-G50 prepared in advance In brief, incubation mixtures of 200μL containing unloaded matrine and RGD-M-LCL were added onto mini-gel columns Then particles of RGD-M-LCL...

Ngày tải lên: 26/10/2012, 08:57

... propagation of Morinda officinalis How Plant Physilogy Comunication 42 (3): 475 He H, Xiao S., Xian J., Xu H (2000) In- vitro culture and plant regeneration of Morinda officinalis How Journal of Guangzhou ... Journal of Guangzhou University of Traditional Chinese Medicine 17 (4): 353-354 291 Quy trình nhân giống in vitro ba kích (Morinda officenalis How) He H., Xu HH (2002) In vitro culture and the Agrobacterium-mediated ... Zhao YM., Luo ZP (2003) Inhibition of the oligosaccharidesextracted from Morinda officinalis, a Chinese traditional herbal medicine, on the corticosteron induced apoptosis in PC12 cells Life Science,...

Ngày tải lên: 28/08/2013, 14:15

... also protein fragments for mapping of interaction domains The use of in vitro expression approaches to analyze protein products is likely to gain momentum for the analysis of known proteins as well ... functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of proteins could be ... original primers Of course in this case the use of an enzyme with 3′-exonuclease proofreading activity should be avoided to protect the differentiating 3′-end An example of the design of original...

Ngày tải lên: 25/10/2013, 22:20