in vitro migration capacity of msc
Báo cáo sinh học: "In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pot
... through binding of CCL2 to its corresponding chemokine receptor CCR2 on the T cells This was demonstrated by blocking of T-cell migration in presence of addition of excess chemokine CCL2 in the ... after adding T cells), fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); ... percentage of apoptotic tumor cells was determined by counting apoptotic nuclei and intact tumor cells in sections stained with H&E Blocking of T-cell migration in reconstruct T-cell migration in the...
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báo cáo hóa học:" In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pptx
... through binding of CCL2 to its corresponding chemokine receptor CCR2 on the T cells This was demonstrated by blocking of T-cell migration in presence of addition of excess chemokine CCL2 in the ... after adding T cells), fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); ... percentage of apoptotic tumor cells was determined by counting apoptotic nuclei and intact tumor cells in sections stained with H&E Blocking of T-cell migration in reconstruct T-cell migration in the...
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Báo cáo y học: "In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains"
... raw rice Discussion We investigated the in vitro antimicrobial activities of the three kinds of Rice-fluid against various species of microorganisms including H pylori strains by the timekill-curve ... phenomena were observed against other four strains of E coli, five strains of S aureus, S epidermidis, E faecalis and P aeruginosa, and three strains of K pneumoniae and two strains of K oxytoca, respectively ... consisting of three kinds of Rice-fluid derived from the two kinds of raw rice; i e., polished (pH6.32) and unpolished (pH5.98), and the usually cocked rice (pH5.91), and remaining one compound of...
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Báo cáo Y học: Differences in the binding capacity of human apolipoprotein E3 and E4 to size-fractionated lipid emulsions pot
... using the direct binding assay as described in Experimental procedures Inset: Linearized plot of the binding data for apoE(263–286) (symbols) shown in panel A The solid line represents the linear ... the binding footprint for the apoE4 isoform, corresponding to 3.0 amino acids per phospholipid, is almost twofold greater than the apoE3 isoform (Table 2), suggesting these isoforms differ in their ... Katsuyama, T (1999) Characterization of apolipoprotein E-containing lipoproteins in cerebrospinal fluid: effect of phenotype on the distribution of apolipoprotein E Clin Chem 45, 1431–1438 55 Funahashi,...
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Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf
... shown in Fig The packaging system contains the self-inactivating transducing constructs (sin) obtained Fig Diagram of lentiviral vector carrying the human IDUA cDNA Gene transfer vector includes ... MluI to obtain the self inactivating (sin) gene transfer construct PRRLsinPPT.CMV IDUA.WPRE The packaging of the vector was obtained as described previously [23,24] by cotransfection of 293T cells ... confluency, and transduced with increasing doses of IDUA vector (1, 10 and 50 ng of p24 viral protein) in the absence or in the presence of polybrene (5 lgÆmL)1) After a 24-h incubation at 37 °C, the...
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Báo cáo khoa học: The in vitro nuclear aggregates of polyamines pot
... ivNAPs in vitro model in order to investigate some of the features of the polyamine–phosphate interactions Specifically, we examined the role played by each polyamine in the self-assembly of in vitro ... by NaCl in conferring stability on these supramolecular aggregates is a rough indication of the degree of difference in complexity between the in vitro and in vivo nuclear settings For instance, ... types of polyamine aggregate are more effective than single polyamines in the carrying out of the crucial functions of the dsDNA protection and conformation, thus indicating that polyamine aggregation...
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báo cáo hóa học:" The effect of abductor muscle and anterior-posterior hip contact load simulation on the in-vitro primary stability of a cementless hip stem" ppt
... 5(3):283-93 37 Cristofolini L, Vinteconti M: Towards the standardization of in vitro load transfer investigations of hip prostheses Journal of Strain Analysis for Engineering Design 1999, 34(1):1-15 ... similar finding was reported in another study, in which stair climbing loads generated approximately 150 μm of distal migration, compared to 30 μm of proximal migration when simulating walking loads ... Measurement of the Relative Motion Between an Implant and Bone under Cyclic Loading Strain 2004, 40(1):193-202 43 Monti L, Cristofolini L, Toni A, Ceroni RG: In vitro testing of the primary stability of...
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Báo cáo vật lý: "Evaluation of In Vitro Antioxidant Activity of 5H-dibenz[b,f]azepine and Its Analogues" ppt
... experiment was performed in the absence of the compound to determine the amount of lipid peroxidation obtained in the presence of inducing agents as a control experiment The percentage of antilipid peroxidative ... preparation of 10-methoxy-5H-dibenz[b,f]azepine (Compound d) 10-methoxy-5H-dibenz[b,f]azepine was prepared by brominating N-acetyl-5H-dibenz[b,f]azepine (2.35 g, 10 mM) using bromine (3.2 g, 20 mM) in ... outlined in schemes 1–3 In scheme 3, to obtain compound (e), the reaction was carried out by using a weak base (triethyl amine) with COCl2 at room temperature (RT) instead of triphosgene in the...
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Báo cáo khoa học: "Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes" docx
... phases However, Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes 69 Ta ble In vitro maturation, fertilization and cleavage rates of bovine oocytes relative to ... levels in the circulation resulting in the regression of dominant follicle and emergence of constant follicle wave turnover [21] The reasons for poor in vitro developmental competence of oocytes ... during in vitro culture The present data are not sufficient to draw any significant conclusions However, it supports the previous findings that renewal of medium every 48 hours during in vitro...
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Báo cáo lâm nghiệp: "In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker" ppsx
... proliferating the axillary shoot within 2– weeks of culture Out of the various cytokinins tested, BAP was the most effective for inducing bud break and growth in axillary meristems Kinetin alone or in ... or in combination for induction of rooting Of the three auxins tested, IBA and IAA induced rooting (Tab IV) The optimum concentration was 0.5 mg/L of IBA or IAA and it resulted in 65 to 70% of ... was presented in Table III The interaction of two cytokinins with low concentration of auxin enhancing shoot multiplication has been reported by many authors [3, 5, 12] The inclusion of higher concentration...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... Aggregates were incubated with 50 μL of the double labelling staining solution for 10 minutes at room temperature After staining, aggregates were washed five times with 100 μL of binding buffer, ... http://arthritis-research.com/content/11/5/R148 Our study is also in agreement with studies of in vitro chondrogenesis with primary MSCs using recombinant proteins, where BMP-4 was identified as a strong inducer of chondrogenesis [36],...
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Báo cáo y học: " In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus" pdf
... plasmids containing HCV NS3 protease in the presence and absence of SN 100 μg extract and interferon by using Lipofectamine™ 2000 (Invitrogen life technologies, Carlsbad, CA) according to the manufacturer’s ... of Dimethyl sulfoxide (DMSO) ensuring stock concentration of 50 μg/μl Sieving the above solution by using 0.22 um filter inside Laminar Flow Hood, storing at -20°C Cell line The Huh-7 cell line ... there is no vaccine available for prevention of HCV infection due to high degree of strain variation The current treatment of care, Pegylated interferon a in combination with ribavirin is costly,...
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Báo cáo y học: "The triple combination of tenofovir, emtricitabine and efavirenz shows synergistic anti-HIV-1 activity in vitro: a mechanism of action study" pdf
... MS: Synergistic inhibition of replication of human immunodeficiency virus type 1, including that of a zidovudine-resistant isolate, by zidovudine and 2',3'-dideoxycytidine in vitro Antimicrob ... NRTI+NRTI combinations was first ignited during the HIV monotherapy era by the surprisingly synergistic effects of AZT+ddI both in vitro and in clinical trial studies [22-24], in the absence of a pharmacokinetic ... effect of TFV+FTC combination, and the statistically significant increases in the levels of the active metabolites in T-cell line CEM[30] To investigate whether the synergistic effect of TFV+FTC in...
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Báo cáo y học: "In vitro nuclear interactome of the HIV-1 Tat protein" pps
... depicted in Figure 5[75233] Global network characteristics Within the interaction map we included interactions involving a minimum of two proteins Non-interacting and self-interacting proteins were ... composed of lamins and NE lamin binding proteins including Nesprin1 alpha, MAN1, lamina-associated polypeptides-1 and (LAP1, LAP2), emerin and Lamin B receptor[254,255] The four latter and Lamin B ... number of appearance and percentage of each of the motifs are shown N Dataset: Number of proteins in Tat interactome dataset possessing motif annotation % Dataset: percentage of proteins in Tat interactome...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... decorin and fibromodulin with transforming growth factor beta Biochem J :527534 24 Iozzo RV 1999;The biology of the small leucine-rich proteoglycans Functional network of interactive proteins...
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Báo cáo y học: "The effect of substance P on asthmatic rat airway smooth muscle cell proliferation, migration, and cytoplasmic calcium concentration in vitro" pdf
... Shanghai, China) Hematoxylin and eosin staining Routine histological staining methods were applied The middle lobe of the right lung sections of 5-μm were stained with hematoxylin and eosin (HE) ... chemotropism [27] In this experiment, we demonstrated that NK-1R antagonist WIN62577 had the effect of inhibiting ASMC migration in vitro, indicating that WIN62577 may contribute to the inhibition of airway ... a measure of [Ca2+]i For each image, regions of interest were defined within single cells, and the average fluorescence intensity of each region of interest was measured The role of WIN62577 on...
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Báo cáo y học: "The effect of substance P on asthmatic rat airway smooth muscle cell proliferation, migration, and cytoplasmic calcium concentration in vitro" potx
... Shanghai, China) Hematoxylin and eosin staining Routine histological staining methods were applied The middle lobe of the right lung sections of 5-μm were stained with hematoxylin and eosin (HE) ... chemotropism [27] In this experiment, we demonstrated that NK-1R antagonist WIN62577 had the effect of inhibiting ASMC migration in vitro, indicating that WIN62577 may contribute to the inhibition of airway ... a measure of [Ca2+]i For each image, regions of interest were defined within single cells, and the average fluorescence intensity of each region of interest was measured The role of WIN62577 on...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding is in concurrence ... statins including inhibition of the metabolism [5], administration of statins with certain juices [6] or inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase ... pravastatin However, the relative impacts of different in vitro findings on the overall in vivo drug delivery efficacy of these cellular carriers remain to be evaluated during the future in vivo...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
... phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, ... containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All of the ... mini-gel columns of Sephadex-G50 prepared in advance In brief, incubation mixtures of 200μL containing unloaded matrine and RGD-M-LCL were added onto mini-gel columns Then particles of RGD-M-LCL...
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Effect of dissolved organic matter (DOM) and biofilm on the adsorption capacity of powdered activated carbon in activated sludge
... time In other word, the adsorption capacity of PAC increased with increase in the amount of organic carbon on PAC The adsorption capacity of PAC after 4th incubation was almost the same as that in ... Adsorption capacity of PAC in aeration tank The adsorption isotherms of new PAC and PAC in the aeration tank for 3,5-DCP are shown in Figure Although the adsorption capacity of the PAC in the aeration ... ranging from 50,000 to 300,000 Da decreased the adsorption capacity of PAC in the aeration tank for 3,5-DCP, the adsorption capacity of PAC in the membrane with molecular weight cut-off of 300,000...
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