the pulmonary mesenchymal tissue layer is defective in an in vitro recombinant model of nitrofen induced lung hypoplasia
The tumour-associated glycoprotein podoplanin is expressed in fibroblast-like synoviocytes of the pdf
... staining, = positive staining of single or limited groups of cells in the lining layer, = continuous positive staining of the cells of the synovial lining layer and = same as 2, but with the ... podoplanin is highly expressed in FLSs of the invading synovial tissue in RA The concomitant upregulation of a-sma and podoplanin in a subpopulation of FLSs indicates a myofibroblast phenotype Proinflammatory ... and the invasion and metastasis of certain tumour cells or tissues [32] Podoplanin has been shown to bind ezrin, an actin filament membrane linker protein, on the inside of the cell in vitro [30,44]...
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Multi-tissue gene-expression analysis in a mouse model of thyroid hormone resistance docx
... genes and WAT In RTH mice this suggests suppression of lipogenesis in the WAT with simultaneous increase in lipogenesis in the heart ing T3 These findings raise the possibility that induction of ... readout for the in vivo actions of T3 and PV in target tissues The animal model was a murine line with the TRβ disrupted in the identical manner as found in the human condition (TRβPV mice) This TRβPV ... 1.9-fold change) thus obscuring the distinction of tissue- specific outliers and excluding potentially important distinguishing gene cassettes In the light of this, we compiled a dataset that lists...
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Báo cáo y học: "Gender-based reciprocal expression of transforming growth factor-β1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis" ppsx
... abnormal voiding and histology characteristic of cyclophosphamide (CYP) -induced cystitis in rats [4] Temporal changes in the levels of pro-inflammatory cytokines and chemokines such as interleukin IL-1α, ... production of TGF-β1 can promote tissue fibrosis in a number of diseases including liver cirrhosis, pulmonary fibrosis, and fibrotic kidney [19] Coincidentally, a significant degree of fibrosis is also ... gender of the animal appears to magnify the differences in urine levels of TGF-β1 and NO2-/NO3- in this inflammatory setting These results support the hypothesis that TGF-β1 can suppress iNOS expression...
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Báo cáo y học: "Gait analysis in a murine model of collagen-induced arthritis" ppsx
... increased in animals with increasing clinical scores (Figure 3, top) The hind-limb paw angle (the angle of the hind paws during peak stance in relation to the long axis of the body and its direction of ... maximal paw area in contact with the treadmill during the stance phase of the step cycle Paw angle The angle of the hind paws in relation to the long axis of the body and its direction of motion Stride ... successful animal model for RA research because it is also an autoimmune model and in many ways resembles RA, including the chronic inflammation [4,14] The primary manifestation of this model is the inflammation-induced...
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Báo cáo y học: " Dimethylthiourea protects against chlorine induced changes in airway function in a murine model of irritant induced asthma" potx
... approved the manuscript HKQ provided critical review of the paper and assistance with data analysis NL assisted in the setup of chlorine exposure and in supervising the exposure of animals in a safe ... of GSH and GSSG and assisted with the writing of the manuscript BD and CW contributed to the revision of the paper and provided the analysis of 4-HNE KG performed the measurements of NOx and approved ... safe manner JJT assisted with analysis of biological samples JGM was involved in the study design, in review of the data and in the preparation of the manuscript All authors read and approved the...
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Báo cáo y học: "Association between inflammatory mediators and response to inhaled nitric oxide in a model of endotoxin-induced lung injury" docx
... whether this interacts with the vasodilating effect of inhaled NO is not clear The analysis of the animal group exposed twice to INO distinguished responders from nonresponders in terms of ET-1 and ... made on the basis of only two mediators (ET-1 and IL-8) may therefore support the hypothesis of a distinct mechanism, independent of the lung damage, responsible for the response to INO The results ... biochemical analysis ML was involved in the study design and in revising the manuscript GH has made a substantial contribution to the design and conception of the study, and to the interpretation of data...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... chondrogenesis of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant ... or remained uninfected For comparison, an additional set of untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or ... formation, of 541.3 ± 166.3 μm2 in the center and 386.1 ± 108.7 μm2 the periphery of the Ad.BMP-2 transduced aggregates, and of 307.8 ± 75.6 μm2 in the center and 248.7 ± 65.4 μm2 the periphery of the...
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– ACT READING TEST PRACTICE – 67. The word exhausted, as it is used in line 23, most nearly doc
... should mean the opposite of origins And because the word beginning can mean origin, choice a is not the right answer Neither crises nor rarity can be the opposite of origins, but peak can, which ... type: inference Choice j is correct The meaning of this phrase can be found throughout the passage, but since the name of the passage is “How the Other Half Lives” and it is about the conditions of ... Choice c is correct The best way to handle this question is to go through the possible answers and eliminate the incorrect ones The first line states that “there is nothing of the giant” in Babbitt,...
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Báo cáo hóa học: " Vaccinia virus lacking the deoxyuridine triphosphatase gene (F2L) replicates well in vitro and in vivo, but is hypersensitive to the antiviral drug " pot
... the incorporation of uracil in their genomes These residues in DNA can arise either through misincorporation of dUTP by DNA polymerase or the spontaneous deamination of cytosine and can result in ... design of the experiments and analysis of the data KAK contributed to the acquisition and interpretation of data RWM contributed to the conception of the studies and the critical review of the manuscript ... to the design of experiments, analysis of the data and the drafted the manuscript ERK contributed to the conception of the studies and the critical review of the manuscript DCQ contributed to the...
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Báo cáo y học: "The renal metallothionein expression profile is altered in human lupus nephriti" doc
... prepared the manuscript MP performed the immunohistochemistry assays and contributed to the analysis of the stained slides CBA contributed to the analysis of the stained slides and validated the tubular ... staining is observed in glomeruli (b) Inner medullary zone of healthy kidney MT-I+II staining is seen in the epithelium of the collecting tubules but not in cross-sectioned thin segments of Henle's ... the current study is based The Novo Nordisk Foundation is thanked for financial support to SJ The IMK Foundation and The Research Foundation of the Danish Medical Society are thanked for financial...
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Báo cáo y học: " Role of the tachykinin NK1 receptor in a murine model of cigarette smoke-induced pulmonary inflammation" docx
... for antigen -induced inflammatory cell influxes in the airway lumen of mice [49] To conclude, the tachykinin NK1 receptor is involved in the accumulation of inflammatory cells in the airways during ... significant Results CS -induced increase of inflammatory cells in BAL fluid and lung tissue Both sub acute and chronic CS-exposure induced an enhanced accumulation of inflammatory cells in the bronchoalveolar ... during the inflammatory response to CS in a mouse model of COPD As inflammation of the airways is an important characteristic of COPD, these findings may have implications in the future treatment of...
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Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
... cannot replace the recruiting function of the Swi1 and Snf5 activator-binding domains The in vivo validation of the Swi1 and Snf5 activator-binding domains is also of interest in relation to the ... binding mechanism between Gal4 and Swi1 that has been characterized in vitro [34], and our results suggest that the coupled binding and folding mechanism is likely to be relevant in vivo This ... binding mechanism is assumed to be generally important for transcription factor interactions with other proteins and may be important for the function of the increasingly large group of proteins...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... determined from the global t The constant a is the ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding ... plot of nuclear NF-jB from in vitro and in silico analysis of the data The plot shows nuclear NF-jB oscillation in the original model and in the updated model with newly measured kr1, ka1 and ... observed in the untreated cell population In order to shed light on the cause of this result, we carried out an in silico analysis by incorporating the inhibition kinetics within the existing computational...
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Báo cáo khoa học: The in vitro nuclear aggregates of polyamines pot
... prepared by changing the concentration of a single polyamine and keeping the concentrations of the other two constant (24 mM) In the case of variation of Spm concentration, the mean ± standard deviation ... ivNAPs in vitro model in order to investigate some of the features of the polyamine–phosphate interactions Specifically, we examined the role played by each polyamine in the self-assembly of in vitro ... preferential disposition of the unimers in a linear chain, rather than their columnar stacking, if the hydrogen bonds are single and arranged in a chain [3] The data reported here concerning the ivNAPs...
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Báo cáo khoa học: ISC1-encoded inositol phosphosphingolipid phospholipase C is involved in Na+/Li+ halotolerance of Saccharomyces cerevisiae pptx
... trp1, his3, can1) and the ISC1 deletion strain DZY1 derived from it (MATa, Disc1::HIS5, ura3, trp1, his3, can1) The HIS5 insertion cassette used for ISC1 disruption was isolated, by short flanking ... run as references isc1D + pDZ6 was a transformant of the isc1D mutant with the ISC1 containing plasmid, pDZ6 cell homogenate The characteristics of the Disc1::HIS5 deletion strain, DZY1, which ... mediates Na+ and Li+ ion extrusion from the cell We therefore investigated whether the loss of halotolerance in isc1D cells was due to the failure of ENA1 induction in the mutant For this, the ENA1-lacZ...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
... covering the burr hole with a piece of gelatin sponge, Page of 10 the temporalis and overlying skin were sutured Animals were then placed in the supine position and a midline incision was made in ... until staining Immunohistochemistry staining Immunohistochemical staining of nestin and BrdU were performed to assess the property of NSC in cultures and track the migration and homing of allogeneic ... relief in ischemic rats given the NSC transplant and the upsurge of NGF in vivo, suggesting an immuno-modulation of the class I MHC by NSC-derived neurotrophins in the microenvironment of the lesioned...
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báo cáo hóa học: " Tumor necrosis factor-mediated inhibition of interleukin-18 in the brain: a clinical and experimental study in head-injured patients and in a murine model of closed head injury." pot
... production in murine brains within 24 hours In contrast, the intracerebral injection of murine Page of (page number not for citation purposes) Journal of Neuroinflammation 2004, 1:13 recombinant TNF (in ... and cytokine measurements in human and murine tissue samples, as well as for writing of the manuscript DP and IY performed the experimental i.c.v injection experiments WE contributed to the interpretation ... interpretation of the results and writing of the manuscript Acknowledgments The authors thank Dr Hans-Pietro Eugster (Division of Clinical Immunology, University of Zurich, Switzerland) for providing the...
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báo cáo hóa học: " Activation of retinal microglia rather than microglial cell density correlates with retinal neovascularization in the mouse model of oxygen-induced retinopathy" ppt
... directly involved in the formation of retinal NV in the later stages of the OIR model In this study, we investigated the temporal and spatial distribution of microglial cells during the different ... guidelines of the Institute for Laboratory Animal Research (Guide for the Care and Use of Laboratory Animals) in accordance with the ARVO Statement for the “Use of Animals in Ophthalmic and Vision ... the deep layer was at the border of the inner nuclear layer and the outer plexiform layer (Figure 1B and 2E) Microglial cell density was always higher in the superficial than in the deep retinal...
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báo cáo hóa học: " Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis" ppt
... expressed as the ratio of the band intensity of the protein of interest to the loading control protein band (b-actin) Isolation of total protein For spinal cord total protein extraction, tissue (100 ... phagocytosis in vitro Since the clearance of debris by microglia is a primordial step for the reparative process in the spinal cord following an autoimmune attack [46,47], the maintenance of a phagocytic ... effector phase of EAE, histological spinal cord analysis was performed in animals treated from the appearance of the first clinical signs At the end of treatment, animals were anesthetized, transcardially...
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báo cáo hóa học:" The effect of abductor muscle and anterior-posterior hip contact load simulation on the in-vitro primary stability of a cementless hip stem" ppt
... (Fm) and anterior-posterior (Fap) loading of the femoral head due to the mounting geometry and the offset between the femoral head and the central axis of the actuator, doff (32 mm) (b) Resulting ... the discussion TO provided important feedback on the statistical analysis and participated in the discussion Author Details 1Department of Mechanical Engineering, Korean Advanced Institute of Science ... Description of application J Biomech 1972, 5(3):283-93 37 Cristofolini L, Vinteconti M: Towards the standardization of in vitro load transfer investigations of hip prostheses Journal of Strain Analysis...
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