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VIỆN HÀN LÂM KHOA HỌC VÀ CÔNG NGHỆ VIỆT NAM VIỆN CÔNG NGHỆ SINH HỌC LÊ THỊ VIỆT HÀ NGHIÊN CỨU C I M HỆ GEN TY TH VÀ N VỊ M H RI OSOME CỦ MỘT S LOÀI SÁN LÁ RUỘT THUỘC HỌ HETEROPHYIDAE KÝ SINH GÂY ỆNH TRÊN NGƯỜI VÀ ỘNG VẬT TẠI VIỆT N M Chuyên ngành: Hóa sinh học Mã số: 42 01 16 LUẬN ÁN TIẾN SĨ SINH HỌC Người hướng dẫn khoa học: GS.TS Lê Thanh Hịa Viện Cơng nghệ sinh học PGS.TS ồng Văn Quyền Viện Công nghệ sinh học Hà Nội, 2021 i M ƠN rưở t t s us hất đ GS.TS Lê Thanh Hoà, nguyên M ễ dị h, V ệ C hệ s h họ , V ệ H m Khoa họ v Ph C hệ V ệt Nam, th o th dành h u th a tru hư v pđ t t t ,đ v s t m hu t đ t đt t h tr th , t o mọ đ u tro qu tr t p hư ệ thu h h d , ợ , trao đ , đị h u, họ t p v ho th h u V ệ trưở Vệ C Nam, th v đồ h sus hư d hoa họ đ t H Vă Qu , Phó V ệ m Khoa họ v C t h hỉ ảo t tro hệ V ệt th a họ t p u h M ễ dị h họ - V ệ C V ệt Nam đ pđ t đượ ảm : hđoVệ C hệ V ệt Nam đ t o đ u - Ba G m đố Quố B th h hệ s h họ h ệt t h - Ba ho ệ ho t đồ s hỗ trợ a (NAFOS ED) tro h H a trùng-C hệ S Đỗ tr th h u rư h m Khoa họ v C h họ , V ệ H h tru v u V ệt Nam, PGS , đồ pđ t tro h ệp - S Ph m B m suốt qu tr h họ h phí Quỹ Ph t tr ể Khoa họ v đ t m số hệ m Khoa họ v C đượ họ t p v ệ u Phòng m h Họ v ệ Y–Dượ họ h, Chủ tị h H -Cm hất t t p thể -VệH hệ s Phòng, Khoa Họ v ệ đ t o đ u u PGS.TS Đồ hệ s h họ , V ệ ảm th đ , hệ Quố v 108.02-2017.09 GS S hủ h ệm ru Dũ ru vệ , rưở v thu m u, thí Khoa K s h tr ,Vệ đồ h ệp Khoa đ h ệm h u h h th Sốt r t-Ký sinh pđ t h họ cho nghiên ii Cuố u tru u t t hu hí h, đ h ệt hu t pt t v ho , pđ sus ũ đ hư h a s th h qu tr h họ t p v h th , h ađ hđ h hă v u m h ê hị V ệt Hà iii LỜI CAM KẾT Tôi xin cam đoan: Đây công trình nghiên cứu tơi số kết cộng tác với cộng khác; Các số liệu kết trình bày luận án trung thực, phần công bố tạp chí khoa học chuyên ngành với đồng ý cho phép đồng tác giả; Phần lại chưa công bố công trình khác Hà Nội, ngày tháng Tác giả năm 2021 iv MỤC LỤC Trang Lời cảm ơn i Lời cam kết iii Mục lục iv Danh mục chữ viết tắt ix Danh mục bảng xii Danh mục hình xiv MỞ ĐẦU CHƯƠNG TỔNG QUAN TÀI LIỆU 1 Tình hình nghiên cứu sán ruột nhỏ h Heterophyidae thuộc lớp Sán Trematoda 1.1.1 Phân loại sán ruột nhỏ họ Heterophyidae 1.1.2 Đặc điểm sinh học phân b c a sán ruột nhỏ họ Heterophyidae 1.1.3 Tình hình nghiên cứu sán ruột nhỏ họ Heterophyidae gi i v Việt Nam 11 1.2 T th , ribosome t th v c i m h gen t th 1.2.1 Gi i thiệu ty thể c a động vật 17 1.2.2 ibosome c a ty thể 21 1.2.3 Cấu trúc v xếp gen hệ gen ty thể 21 1.2.4 Đặc điểm gen v v ng kh ng m h a hệ gen ty thể 23 1.2.5 Nghiên cứu ứng dụng gen v hệ gen ty thể 29 1.3 Giới thi u Ribosome c a t 1.3.1 Gi i thiệu ribosome c a tế b o 31 1.3.2 Đơn v m h a ribosome 33 1.3.3 Nghiên cứu ứng dụng ch ov 17 n v m h a ri osome .31 th phân t c a đơn v m ribosome 37 v 1.4 ngh a v s c n thi t nghiên cứu c i m mtDNA v v m h a ri osome c a sán ruột nhỏ Haplorchis c a CHƯƠNG ĐITƯNG n ti 40 VẬT LIỆU VÀ PHƯƠNG PHÁP NGHIÊN CỨU ……………………………………………………………… 43 2.1 Đit ng v t li u a i m nghiên cứu ……………………… 43 2.1.1 Đit ng v vật liệu nghiên cứu ………………………………… 43 2.1.2 M u nghiên cứu c a lo i thuộc họ Heterophyidae ………… 43 2.1.3 Đ a điểm v thời gian nghiên cứu 45 2.2 Ph 45 2.3 Ph 2.3.1 Ph ơng pháp xác đ nh cấu trúc v xếp hệ gen ty thể c a sán ng pháp ti p c n v tr nghiên cứu …………………… ng pháp nghiên cứu h gen t th ……………………… ruột nhỏ Haplorchis taichui ……………………………………… 2.3.2 Ph ơng pháp phân t ch đặc điểm gen N Ph ơng pháp phân t ch đặc điểm gen N 47 ribosome (MRG) ty thể ………………………………………………………………… 2.3.3 47 vận chuyển t N 48 ty thể ………………………………………………………………… 48 2.3.4 Ph ơng pháp phân t ch v ng kh ng m h a hệ gen ty thể …… 49 2.3.5 Phân t ch ph ơng thức s dụng nucleotide v t nh toán giá tr độ lệch skew skewness hệ gen ty thể ……… ……………… 2.3.6 Phân tích đặc điểm gen m h a protein v s thiên v s dụng m …………………………………………………………… 2.3.7 Ph Ph Ph 2.4 Ph 51 ơng pháp phân t ch phả hệ d a ch th cộng h p ba gen PCG c a ty thể (cob+nad1+cox1) ……………………………… 2.3.10 50 ơng pháp t nh toán khoảng cách di truy n c a lo i sán ruột nhỏ d a cộng h p 12 PCG c a ty thể ………………… 2.3.9 50 ơng pháp t nh toán khoảng cách di truy n c a lo i sán ruột nhỏ d a cộng h p gen cob+nad1+cox1 ……………… 2.3.8 49 51 ơng pháp phân t ch phả hệ d a ch th cộng h p to n 12 PCG c a ty thể ……………………………………………… 52 Ph 53 ng pháp nghiên cứu n v mã hóa ribosome (rTU) vi 2.4.1 Ph ơng pháp xác đ nh cấu trúc v xếp gen v ng giao gen c a đơn v m h a ribosome 53 ………………………………………… 2.4.2 Ph ơng pháp xác đ nh độ d i v đặc điểm gen r N v v ng giao gen đơn v m 2.4.3 skew đơn v m h a ribosome …… 54 Ph ơng pháp phân tích t ơng đ ng nucleotide c a gen r v ph n m 2.4.5 h a ribosome c a sán ruột nhỏ họ N eterophyidae 54 Ph ơng pháp phân tích đặc điểm cấu trúc bậc hai gen r N ribosome (5.8S, 18S, 28S rRNA) ………………………………… 2.4.6 Ph ơng pháp phân tích phả hệ c a H taichui v Ph ơng pháp phân tích phả hệ c a H taichui v CHƯƠNG KẾT QUẢ NGHIÊN CỨU ………………………………… 57 58 K t qu nghiên cứu h gen t th loài Haplorchis taichui m u Vi t Nam ………………… 3.1.1 56 H pumilio d a cộng h p gen (18S+28S rRNA) to n ph n 3.1 56 H pumilio d a chu i gen 28S rRNA to n ph n …………………………… 2.4.8 55 Ph ơng pháp phân tích đặc điểm cấu trúc bậc hai v ng giao gen ITS (ITS-1, ITS-2) ……………………………………………… 2.4.7 53 Ph ơng pháp phân t ch ph ơng thức s dụng nucleotide tính tốn giá tr độ lệch 2.4.4 h a ribosome 58 Cấu trúc v xếp hệ gen ty thể c a sán ruột nhỏ Haplorchis taichui 58 3.1.2 Phân t ch đặc điểm gen N ribosome c a ty thể ……………… 62 3.1.3 Đặc điểm gen N vận chuyển t N 63 3.1.4 Đặc điểm v ng kh ng m c a ty thể ……………… h a mtDNA c a sán ruột nhỏ Haplorchis taichui ……………………………………………… 3.1.5 Ph ơng thức s dụng nucleotide v giá tr độ lệch skew hệ gen ty thể ……………………………………………………… 3.1.6 Phân t ch đặc điểm gen m h a protein v thiên v 65 65 s dụng m (codon usage) ………………………………………………… 66 vii 3.1.7 Khoảng cách di truy n Haplorchis taichui v lo i sán khác họ eterophyidae v Opisthorchiidae d a cộng h p amino acid c a ba gen (cob+nad1+cox1) ……………………… 3.1.8 Khoảng cách di truy n Haplorchis taichui v lo i sán khác họ eterophyidae v Opisthorchiidae d a cộng h p amino acid c a 12 PCG ………………………………………… 3.1.9 K t qu nghiên cứu 77 Cấu trúc v xếp gen c a đơn v mã hóa ribosome c a Haplorchis taichui 3.2.2 74 n v m h a ri osome loài Haplorchis taichui v lo i H pumilio m u Vi t Nam ……………………… 3.2.1 72 Phân t ch phả hệ sán ruột nhỏ d a chu i amino acid cộng h p c a to n 12 gen m h a protein ………………………… 3.2 70 Phân t ch phả hệ sán ruột nhỏ d a chu i amino acid cộng h p c a ba gen m h a protein (cob+nad1+cox1) ……………… 3.1.10 68 Độ d i gen r N v ………………………………… 77 v ng giao gen đơn v m h a ribosome c a lo i Haplorchis taichui v Haplorchis pumilio 80 3.2.3 Đặc điểm cấu trúc bậc hai gen r N 81 3.2.4 Đặc điểm cấu trúc v ng giao gen TT -1, ITS-2) …………… 86 3.2.5 ết phân t ch ph ơng thức s dụng nucleotide đơn v m h a ribosome ………………………………………………… 88 3.2.6 ết phân t ch t … v eterophyidae … 90 Phân t ch phả hệ c a H taichui v H pumilio d a chu i nucleotide gen 3.2.8 r N ơng đ ng nucleotide c a gen r N ph n m h a ribosome c a sán ruột nhỏ họ 3.2.7 ribosome to n ph n ………………………… 91 Phân t ch phả hệ c a H taichui v H pumilio d a cộng h p to n ph n hai gen rRNA (18S+28S rRNA) ……………………… 94 CHƯƠNG BÀN LUẬN KẾT QUẢ …………………………………… 97 4.1 99 V nghiên cứu h gen t th sán ruột nhỏ Haplorchis taichui viii 4.1.1 V thu nhận liệu gen học hệ gen ty thể ……………………… 4.1.2 V đặc điểm hệ gen ty thể c a sán ruột nhỏ Haplorchis taichui 4.1.3 V s ứng dụng mtDN nghiên cứu di truy n v phả 4.1.4 hệ ………………………………………………………………… V đ ng g p cung cấp sở liệu hệ gen ty thể ……………… 4.2 V nghiên cứu n v m h a ri osome H.taichui v 100 104 107 H pumilio …………………………………………………………… 4.2.1 V thu nhận liệu gen học đơn v m h a ribosome …………… 4.2.2 V đặc điểm đơn v m h a ribosome c a sán ruột nhỏ Haplorchis taichui v ………………………………… 99 109 109 H pumilio 110 4.2.3 V s ứng dụng rTU nghiên cứu di truy n v phả hệ … 113 4.2.4 V đ ng g p cung cấp sở liệu đơn v m h a ribosome … 116 KẾT LUẬN VÀ KIẾN NGHỊ 119 NHỮNG CƠNG TRÌNH ĐÃ CƠNG B LIÊN QUAN ĐẾN ĐỀ TÀI 121 TÓM TẮT BẰNG TIẾNG ANH 122 TÀI LIỆU THAM KHẢO 130 PHỤ LỤC ix DANH MỤC CÁC KÝ HIỆU, CÁC CHỮ VIẾT TẮT Chữ viết tắt Nội dung i i ngh ATP Adenosine triphosphate Phân tử mang lượng bp Base pair Cặp bazơ Consensus sequence cs Chu i chung la t ch nh nhi u chu i cộng DNA Deoxyribonucleic acid Axit Deoxyribonucleic ĐVLSN Zoonosis/zoonoses Động vật lâ sang người et al ETS hai pin /stem người khác External Transcribed Spacer Vùng ngoại gen Secondary structure Cut c nhánh HTH Morphology IGS Vùng l ITS Non-transcribed intergenic spacer Integrated Taxonomic Information System Internal Transcribed Spacer Kb (= Kbp) Kilo base pair Đơn vị đo (= 1.000 cặp bazơ) KCDT Genetic distance KST Parasite LNR Long non-coding region Vùng khơng mã hóa dài Secondary structure Cut Long- Polymerase Chain Reaction Phản ứng chu i t ùng hợp dài ITIS loop LPCR nh thái h c th ng th ng tin phân loại t ch hợp Vùng giao gen hoảng cách di t u n sinh t ùng c nh nh i n gi i g n ùng gi o g n củ mtDNA hoàn ch nh ( 5.120 bp) oài Haplorchis taichui củ iệ N (chủng Htai-QT3-VN/GenBank: MG972809) cox3(645) * * 20 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 AGGTGGTTACCTGTTTATAATTCTTGAGTGGTTATGGTGGGTATAGTAAGTTTGTTTTTGTGAAAGCTTTGGGGCGTTTTTTTCTTTTTGGGTGCGTTGATTTTTTCGGCTTATTTTCTTGTATGAGAGGTTTTTTTTCCACATTTAAGGCGGTATGCTATGGCTTTTTGACTATTT : SWLPVYNSWVVMVGIVSLFLWKLWGVFFFLGALIFSAYFLVWEVFFPHLSRYAMAFWLF * * 200 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 HtaiQT3-mt : ATAGTTAGTGAGGTGGCTATTTTTGGGAGGTTGCTTTTAACTGTTCTTTGAAAAGATGACGGTGGGGAGGGGGCCTTGTCGGATAGCTTGGAGTTGCCTTTTTTAGGGTGTTTTCTTTTGTTGACTTCCTCTTTGACGGCCACAGTGTATCATCATTGTTTTGGCTTGCCTTATAGTTGG : IVSEVAIFGSLLLTVLWNDDGGEGALSDSLELPFLGCFLLLTSSLTATVYHHCFGLPYSW * * 380 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 HtaiQT3-mt : GTTTTTTTACTTATCAGTATCTTTTTAGGGTTTGGGTTTGTGTGTCTGCAGTTGTGGGAGTTTTTTGATTGTGAGTGTGATGTTCTTTATTGTACTTATTTTGGGGCTGCTTTCTGTACTGTTGGGTTGCATTTTAGTCATGTGGTTGCGGGGGTCGCTGCTATGACCGTTTTGTTAGTG : VFLLISIFLGFGFVCLQLWEFFDCECDVLYCTYFGAAFCTVGLHFSHVVAGVAAMTVLLV HtaiQT3-mt : ATG M trnH(His)(66) 180 360 540 cob (1110) * 560 580 * 600 * 620 * 640 * 660 * 680 * 700 * 720 HtaiQT3-mt : AGGGGTTGGGATACCAATCATTTTTATGCTAGGGTGGTTGTGTGGTATTGGCATTTTGTTGATTATGTGTGGTTGTGAGTATACCTGTTAATATATTACGTT TAGGG TCTTCAATAGGTTATTTAAAACTGTTGTCTTGTGGAGACAGAGATTCGTTGTGTGTGATTGAGGAA GGTTTA A : 720 SGWDTNHFYASVVVWYWHFVDYVWLWVYLLIYYV * * * 740 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 HtaiQT3-mt : TG GTTTCTGTTGTTCGTGGTAAAGTAGTGGATCTTCCTACTAAAGTTTCTTTGAGGTATTTTTGATGTGGGGGGTTTATGATTAGTGTGTTTTTAGTCATTCAGGTTGTATCTGGTGTAATTCTGTCTTTGCTTTATGTAGGAGATGCGGGGGTGAGGTTCGGGTTGGTGCTGGATTTTG : 900 M VSVVRGNVVDLPTNVSLSYFWCGGFMISVFLVIQVVSGVILSLLYVGDAGVSFGLVLDF * * 920 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 HtaiQT3-mt : TTAACGAGAGTTTGTTTTTATGATTTGTCCGTTATTTGCATATATGGGGAGTAACGTTTATTTTCCTACTGTTCTTGGTTCACATGGGGCGTTCTTTTTATTATTCTAGTTATAGGAAGTTGGGGGTATGAAAAGTTGGGTTCGTTCTTTATATTTTAATGATGATTGAGGCGTTTTTAG : 1080 VNESLFLWFVRYLHIWGVTFIFLLFLVHMGRSFYYSSYSKLGVWNVGFVLYILMMIEAFL * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 HtaiQT3-mt : GGTATATTCTTCCGTGACATCAGATGTCCTATTGGGCTGCCACTGTTTTAACCTCGATACTTAAAAGTATACCAGTTGTAGGCGGTAAGCTTTATGAGTTTATTGTTGGGGGCTTTGGGGTCACTAAAGTGACTCTAGTCCGTGTTTTTTCAGCTCATGTGTGCCTGGCTTTTGTGATAA : 1260 GYILPWHQMSYWAATVLTSILNSIPVVGGKLYEFIVGGFGVTNVTLVRVFSAHVCLAFVI * * 1280 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 HtaiQT3-mt : TTGGGCTGAGTGTGATTCATCTTTTTTATTTGCATAAGGGAGGGTCTAATAATCCTTTATCTGCGCCTGGGGGCTACAGGGACGTTGTCCTGTTTCATTCATATTTCACTAGAAAGGATGGGGCGGTGCTTGTATTATTGTTATTACTCCTGGGAGGAGGGTTGATGATATCGCCAGACG : 1440 IGLSVIHLFYLHKGGSNNPLSAPGGYSDVVLFHSYFTSKDGAVLVLLLLLLGGGLMISPD * * 1460 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 HtaiQT3-mt : CTGTTCTAGATGTGGAAAAGTATATTGAGGCGGATCCGATGGTTACTCCTGTGTCAATAAAGCCTGAGTGGTATTTTCTTGCTTTTTATGCGATGCTGCGGTCTATAGAGTCGAAGGTTGGGGGCTTGGTGCTGGTTTTGATATTCCTGTTCGTTTTGTGATTACCTACTTTTAATTCCT : 1620 AVLDVEKYIEADPMVTPVSIKPEWYFLAFYAMLRSIESKVGGLVLVLIFLFVLWLPTFNS * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 HtaiQT3-mt : CTTGTGTTTATAGTGTGTTGCGTCAGTATGTATTTTGGGGCTGTTGTTCTCTTTACTTCATTTTGAGCTATCTTGGTGCTTGTCACCCCGAATATCCTTTTGTTTTTATAAGCAAGTTTTGTTCTCTCTTGATTGTTGTATTATTAATGCTGTTTAAGGGGCTGTGGGCAGTTCCTTATT : 1800 SCVYSVLRQYVFWGCCSLYFILSYLGACHPEYPFVFISKFCSLLIVVLLMLFKGLWAVPY * nad4L(264) * * 1820 HtaiQT3-mt : CTGGGGGGTTTCCTCGTTTGGTTTTG TAGT ATG SGGFPRLVL * M 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 GTTCGGTTGCTTTTGCTTTTAGGCATTTTTATAATTTTGTGTAGGTTTTTTCTTTCTTTGACGCGCTTGTTGAAATGCCTAATAGTGGTTGAGAATTTTAAAGTGTTGGTGTTATTTGTGAGTTTATTATCCCAGCGGGGTGAGACT : VRLLLLLGIFIILCSFFLSLTRLLNCLIVVENFNVLVLFVSLLSQRGET FILFIALMVIFTIEVILGLVVLTRL * 2180 W * 1980 nad4(1281) (40 bp overlapping with 3’ end of nad4L) * 2100 * 2120 * 2140 2160 GGGCCTTGTCGGTTAGTGGTTTGATTATTTTGGTTGAGGCCTGTGCAGGGTTGGATAGAATCTGGC : * 2000 * 2020 * 2040 * 2060 * 2080 * HtaiQT3-mt : TTTATACTTTTCATTGCCTTGATGGTGATTTTTACTATTGAGGTTATTCTTGGTTTGGTGGTTTTGACCCGGTT GTG GGATTCAAGAGGTTTAGTTGGTATAGTGGGTTGATAG 2200 * 2220 * DSSGLVGIVGW V 2240 * 2260 2300 * 2320 * 2340 HtaiQT3-mt : TTAGCCATTCTGGGGTTACGGCGGGAGTTTTTTGTTTTGATGTTATAAGCTTTTATCTGATTCTGCTTTCAATTTTATTATCCTTGTCTTTAAGTTTTTGGGTTGAGAGTATGAGATATTTTAGACGCTTTATGATTTTCTTGAGAGTGCTAGGGTCGCTTTTATGTTACTGTTGTGTTC LSHSGVTAGVFCFDVISFYLILLSILLSLSLSFWAESMSYFSRFMIFLSVLGSLLCCCCV * * 2360 * 2380 * 2400 * 2420 * 2440 * 2460 * 2480 * 2500 2520 HtaiQT3-mt : ATGTCGTTTGGTTTTGAGTTTTTTATGAGATGTCAATAGTTCCGTTGCTTGTGTTGTTAATTGTAGATTCTCCTTATTCGGAGCGTTATGTTGCTTCTTGATATCTTTTGGGGTATGTGGTTTTTACGAGTCTCCCAATGTTGCTGTGTATACTTTATTTGTCTCTACTTGAGGGGGGGT HVVWFWVFYEMSIVPLLVLLIVDSPYSERYVASWYLLGYVVFTSLPMLLCILYLSLLEGG * * 2540 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660 * 2680 2700 HtaiQT3-mt : TTAATATGGGGTGTTGGGGGTCTTCTGATAGGTGGGTTGTAACCGGGAGGTTAATTGTTTTAGCCTTTCTTTTTATTACAAAGATACCTTTGCCTCCGTTTCATGTGTGGCTTCCGATAGTTCATGCTGAGGCTAGGAGCCCAGTATCTGTCTGTTTGAGGGGGTATATAATGAAGTTGG FNMGCWGSSDSWVVTGSLIVLAFLFITKIPLPPFHVWLPIVHAEASSPVSVCLSGYIMKL * * 2720 * 2740 * 2760 * 2780 * 2800 * 2820 * 2840 * 2860 2880 HtaiQT3-mt : GTTTACTTGGTGTCTGCCGGTTTTGTTTTTTTTTACTTCCCGATTTTATTTTCTCTTTGGCTTATACGGTTATATGCTTTTTATGTTCTGTGCTCTTCTTCTTTTCTGCTAGTCGGGAGTTGGATGGTAAGCGCTGACTTGCTTTTTTAAGTCTTTCACACATTTTAGTGGCTATGGGCT GLLGVCRFCFFLLPDFIFSLAYTVICFLCSVLFFFSASRELDGKRWLAFLSLSHILVAMG * * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 3060 HtaiQT3-mt : GTCTGTCTGTGGGGGGTTGAGTATTGAGGGGCTCAGCTATTTTGTATTGTATGGGTCATGGGCTGTCTGCGGGGGTGATGTTTTTATTTCTTTGGTTGATGTATGATGTGGTGGCTACTCGTAATTTAGCTTTGTTGAAGTCGGGGATTTCGGGAAGTTTGTTGTTTCGTGTGTTGGCTT CLSVGGWVLSGSAILYCMGHGLSAGVMFLFLWLMYDVVATRNLALLKSGISGSLLFRVLA * * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 3240 HtaiQT3-mt : GTTGCTGTTTATGTACTGCTTGTTCTTTGCCCCCCACAGTACAATTTTTTTGTGAAGTTTCGTTAATGACTGATGGGGCCTGGAGGGGGGGTTTCTTTTTACTAGTTTTTTATGCTTATGTCTTTATGGGAGGCTTGATGCCTTTATTTTTAGTAGGAAGGTTATTGAGCCGGCATTATG CCCLCTACSLPPTVQFFCEVSLMTDGAWSGGFFLLVFYAYVFMGGLMPLFLVGSLLSRHY trnQ(Gln)(66 : 2340 : 2520 : 2700 : 2880 : 3060 : 3240 trnF(Phe)(64) ) * 3260 * 3280 * 3300 * 3320 * HtaiQT3-mt : ATGTGAGGTTTCATGGGGGTTCAGCAATCGGTGTAAAGGTTTATTCTTTATTCTACTTGATTATGTGGAGGTTTGGCTTATTTCTGGTATGT DVSFHGGSAIGVKVYSLFYLIMWSFGLFLVC 2160 * GFKSFSWYSGL IGALSVSGLIILVEACAGLDSIW * 2280 * 3340 * 3360 * 3380 * 3400 3420 TAAATTGGGGTT TATAGCTTGGTGTAGTTAGAAGCATGCTATGTTTTGGTTGTAGCGGGGATTGGATGTTAGCTATAG TGT TTCCGCC : * * trnM(Met)(64 )(1 bp overlapping with trnF) 3420 atp6(516) * 3440 * 3460 * 3480 * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 HtaiQT3-mt : TAGCTTAAATTTAAAGCGTTAGTTTGAAGAGCTGAAGATATTATATTGTGGTGGGA G GCGGGATAAGTTAAGTGGGAAACTCTATGGTTCATGTTCATAAAATGCGTGTAGCTCCTGCCT ATGTGTTTTTCCCGTGTGAGAAGAGTTTATAATAATGTGATTAGATATGTGGCGGGGGGA : 3600 M CFSRVSSVYNNVISYVAGG * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 * 3780 HtaiQT3-mt : TTGGGAGATAGATTTTATCGTTTACTTCTTTTTATGGTGTTGTTAGTTTTTTTGGGGTTACGGCTTCCTTATTTTTATGGGATGGGGGGATTTGGTATTTTTATTTTGATTAGGGTTTTCCCCCTTTTTATGAGTTTGTTTTTTTCTCGTTTGATTGATGGTAGTATGAGTGCGTTTTTT : 3780 LGDSFYRLLLFMVLLVFLGLRLPYFYGMGGFGIFILISVFPLFMSLFFSRLIDGSMSAFF * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 HtaiQT3-mt : TGTGGCTTTGTTCCTCAGGGTACTCCGTTGTGGATAGCTCCGTTTGTTTGTTTGGCTGAAACTCTAAGTTATGTTGTGCGGCCCGTGGTTTTGATGATACGTCCTTTTGTAAATCTGAGTATTGGAGCTTTAGGGGGGGCAGTATTGGGTGCTTTAAGTATGGCTAGAGGTTGAGTTTTA : 3960 CGFVPQGTPLWIAPFVCLAETLSYVVRPVVLMIRPFVNLSIGALGGAVLGALSMASGWVL nad2(870) * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 HtaiQT3-mt : TTTTTTTTGGTTCTTTTATTTTTTTATGAGGTCTTTGTCGCGTTGGTTCATTGATTTATCGTGTGCAGTATCCTTTCTTTTTCGGAGGATCAC TAG AAGGGGGGGGGGTAATCGGGCTTAG ATG CGCGGGCTGGCGTTATCTGGTTTTAGGGTGGTCAGTGTTTTCTTGTTCTCTTTATG : FFLVLLFFYEVFVALVHWFIVCSILSFSEDH * M RGLALSGFSVVSVFLFSLC * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 HtaiQT3-mt : CTTGTTCTTGAGAGATAATCTTTTATTATTCTGGCTGTTTTTGGAACTTGGCGCTTTGAGGTTGGTTCCTGGATTTTTTATAGTTGGTGGGAGGGGCGCCTTGGGCGGTCTTTTTAGCTACATTTTGGTTTCTGCGGTTTCTTCTTCGTTAATAGTAAGGGGACTGTTATACGAGGATGT : LFLSDNLLLFWLFLELGALSLVPGFFIVGGSGALGGLFSYILVSAVSSSLIVSGLLYEDV * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 HtaiQT3-mt : TTTTTTTCTGTCTTGTTTGGGATTGATAATGAAGTTTGGGGTTTTCCCCTTTATGGGTTGAGTATATGTTGTTGTTTTGGCTAGCAACTGGTTAGTAATATGATTGCTTTCGACTGCTTTAAAGATGGGTTTTTTCTTTTTTTGTTTTTTTATTAGGAGGGGGTGAGATTCTTTGCTTGT : FFLSCLGLIMKFGVFPFMGWVYVVVLASNWLVIWLLSTALKMGFFFFCFFISSGWDSLLV * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 HtaiQT3-mt : AATGATGTTGTGTGTGTTTACCTTTTTGGTGTTGAGTCTGTTGTTTTGGGTTTTTACTCCTAGTTGACTGGTTTATTGGTGTCATGTCTTGATTAGATCTAGGGCTTCTTTGATACTAATGGCTTTGGAGGTTTCAGTTGATTTACTTTTATGACTGTTTGTTATTTATTTTGTGTGGTC : MMLCVFTFLVLSLLFWVFTPSWLVYWCHVLISSSASLILMALEVSVDLLLWLFVIYFVWS * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 HtaiQT3-mt : TACTTGTGCTATTATGCTTTTACGTAGGCTTAGCTTGATGAGCCTGGGTAATAGCGTGGTGGTTTTTTGGGTCGTGTTTATTTTGGTGGCGTTTCCTTTCTCTGTTTCAATTTTTTATAAGTTGGTTGTTAGTCTTTGTATGTACTCGTGTGGTGTTTTAGCATTGTTGGGGTGGGTTGT : TCAIMLLRSLSLMSLGNSVVVFWVVFILVAFPFSVSIFYKLVVSLCMYSCGVLALLGWVV trnV(Val)(60) trnD(Asp)(67) * 4960 * 4980 * 5000 * TAA GGGAGGCGAGATAGTTTATTGAAAATGTCTATTTTACGCGTAGAAGAAGGATTACCTATCGTCA * G 5020 * 5040 AACGATATAGTTTAATAGGAGATG : HtaiQT3-mt : HtaiQT3-mt : 4680 4860 5040 nad1(906) * 5060 * 5080 * 5100 * 5120 * 5140 HtaiQT3-mt : GCCGCTCTGCAAGCGGTCGGTGAAGGATTTCTATCGTTA GCCTGTTCTTAGTTTATTGTAATAATAGCTTGTCGTGCTATAGGAGGGTGGCTAGACACTCAGAGCGGG HtaiQT3-mt : 4500 trnA(Ala)(63) * 4880 * 4900 * 4920 * 4940 HtaiQT3-mt : GTATAAAGTTTCGGAGCAGTTTTTTTTAGTTAAGTATATGGTGAGAGAAGGTATTCCCCGTGCGGAGTGAAGAGGAGTAGGTGTGGTT YNVSEQFFLVKYMVSEGIPRAEWSGVGVV HtaiQT3-mt : 4140 4320 * 5160 * 5180 * 5200 * 5220 GTG ACTTGGTTGTTGGGCGTTTATAATTTTCTTAGGTCGTTTTTGGGGTTTGCGGTGATAATGATTTTTGTA : V TWLLGVYNFLSSFLGFAVIMIFV * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 GCCTTCTTTATTTTGGGGGAGCGCAAGGTTTTGGGTTATATGCAGATTCGTAAGGGTCCGAACAAGGTGGGGCTTGTAGGGCTTTTTCAAAGGTTTGCCGATTTGTTGAAGTTGATAATAAAGTTTAAGGTTAGCCTTTATCAGGTTCGTAGGTGACTCTCTTGAATTGGGGTTTATCTT : AFFILGERKVLGYMQIRKGPNKVGLVGLFQSFADLLKLIIKFKVSLYQVRSWLSWIGVYL * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 TTGTTATTTTTGGCGCTTTGTTATTGTTTTGTTTTTGGGTTGTTTTATGGTGGTGTGAGTTGTGTAAAAGGTTTGCTTTGGGTATTGGTGGTAACGAGTATAACGGGGTATAGTCTTTTAAGCATTGGTTGAGGGTCTTATAAAAAGTATGCTTTGGTTAGTTGTGTCCGTTCTGCTTTT : LLFLALCYCFVFGLFYGGVSCVNGLLWVLVVTSITGYSLLSIGWGSYNKYALVSCVRSAF * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 GGGTCGGTGACTTTTGAGGCGTGTTTTATGTGTATTGTGGTGATTGTGGGTGTAGTCTGTGGGGGTTACTTTGGTTACTCGGTGATGGAGAATGCGTGGTTAGTCTTGTTGGTGAGACCTTTAGGATATGCTTTATGATTAGTGGGGATGTTATGTGAGTGTAAACGAACACCTCTAGAT : GSVTFEACFMCIVVIVGVVCGGYFGYSVMENAWLVLLVSPLGYALWLVGMLCECNRTPLD * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 TATGCGGAAGCTGAGAGGGAGCTGGTGAGAGGGTTGAATACGGAGTATTGTAAAGTGCCTTTTACTTGCTTGTTTGCTTGTGAGTATCTGATAATGTTTATTTTTTCTTGGTTGGGGGCTTTATTATTTTGGGGAGGGGGCCTAATTTCGTTTTTTAGGTTTGTTCATGTTTTGTTTTTT : YAEAESELVSGLNTEYCNVPFTCLFACEYLIMFIFSWLGALLFWGGGLISFFSFVHVLFF 5220 5400 5580 5760 5940 trnN(Asn)(66) * 5960 * 5980 * 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 HtaiQT3-mt : ATTTGGGCGCGTGCAACTTTACCTCGAGTGCGGTATGATTATTTTGTGGCTTTTATGTGAAAGTACTCAATCCTTATATTGGTTTTTTCGTTATTTTTAGTGGTGTTAGTT TAG TG GTGTGGGTAGATTAATGGTAAAATCGTTAAGCTGTTAACTTAAAGATGATCGGGTATCCTCAGA : IWARATLPRVRYDYFVAFMWKYSILILVFSLFLVVLV * trnP(Pro)(65) HtaiQT3-mt : CG nad3(360 trnI(Ile)(65) (4 bp overlapping with trnP) 6120 trnK(Lys)(65 ) * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 * 6260 * 6280 * 6300 AGG CAGTCGTATAGTCTATTTTAGGATGTTAGCTTTGGGGGCTTAAGGACTCTTGAGGAGTGGG CTGA TAGGGCTGCTGAGCAGGCCACTGTGATATAGTGGTTGTAGGAGAATTAGATCCTCGTCGGT AGA CTGTGGGTAGCTTAAGGTGTAAAGTGTTAGATTCTTACTCTAGTGA : 6300 ) 6320 * * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 HtaiQT3-mt : TGTTTTTGAACTCCATAGG ATG GGTTATTCTGGCTTGTGTGCTCTTTTATTCTTTTTGATTTTTTTTTTGGTCGGTGTATTCCATTTATTTTTATGGGATTCTGGTTTGTGAGGGTTTGTAAGTGGGTTTCGGTCTTGGGTCGGTAGGTTTGAGTGTGGTTTTATCTCTCAGGGGGTGTC : 6480 6500 * * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 HtaiQT3-mt : AGAGAAATATTTTAGGTGAACTTACTTTATACTGCTAGTCTTTTTTGTTGTTTTTGACTTGGAAATTTCGTTACTTCTAAAATTACCTTTACAAGGGCTGCTTTTTAAGAAATTGCCCTATTATGTGTGTTTTGtGGTACTCCTGTGCTTTGGTTTTGGGATTGAGGTGAATAAGGGTTA : 6660 trnS1(Ser1)(AGN)(62) trnW(Trp)(64) cox1(1542) * 6680 * 6700 * 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 HtaiQT3-mt : TGTCGAGTGAAGCTAC TAG GGGTTAGATGTGT GAGAGCTATGTGAAGCTGTTGCTGCTAACAATGGTAAGAGTGTTTAAAGCGCTCGGCTCTCTTGTAGGG AACAATCTAGGTTAAGTAGAGACTGTCTGTCTTCAAAACAGAGGGTAGCTTGCGCTGTTTGTTGTTAG ATG TTGACTTG : 6860 * * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 * 7020 HtaiQT3-mt : ATTATTGACTTTGGATCATAAGCGAATAGGGTTGATATATATGATTGTTGGAGTGTGAGGGGGCTTTTTAGGTCTTTCTTTGAGGTTTTTGATCCGTGTTAACTACTTGGATCCTTACTATAATGTTGTATCCCCAGAGGTTTATAATTATGTAATTACTAGGCATGGTATTGCGATGAT : 6840 7020 * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * HtaiQT3-mt : TTTTTTTTTCTTAATGCCGGTTTTGATTGGGGGGTTTGGAAAATATCTGTTGCCCCTGTTGTTGGGGCTTCCTGATTTGAATTTACCCCGTTTAAATGCCCTTAGAGCTTGGTTAATGATTCCTTCTGCTGTTTGTTTTGGGTTAAGTATGTTTGGGGGGTCTGGGGTTGGCTGGACTTT * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 * 7340 * 7360 * HtaiQT3-mt : TTATCCCCCCCTGTCTTCTAGGGATTATAGGGGTTGGGGTGTTGACTTTCTTATGTTTTCTTTACATTTGGCGGGGTTATCTAGTCTGTTTGGTTCTTTAAACTTTATTTGTACAATAATGAGGGCTATGAGTAATGATACCAGGGAGCGGTACTCTATTGTGGTTTGGGCTTATTTGTT * 7400 * 7420 * 7440 * 7460 * 7480 * 7500 * 7520 * 7540 * HtaiQT3-mt : TACTTCTATTCTTTTGTTGCTTTCGTTACCAGTGCTAGCTGCGGGTATAACGATGCTTTTGTTTGATCGTAGATTTAGCTCTTCTTTTTTTGATCCATTGGGAGGGGGGGACCCTGTCTTGTTCCAGCATATGTTTTGGTTTTTTGGGCACCCCGAAGTTTACGTTCTTATTCTCCCAGG * 7580 * 7600 * 7620 * 7640 * 7660 * 7680 * 7700 * 7720 * HtaiQT3-mt : GTTTGGTATAGTGAGACACATTTGTAGGACGTTAACAAATAAAGATTCCTTGTTTGGTTATGGGGGTTTAGTTCTTGCTATGTTTTCTATAGTCTGTTTGGGGAGTGTTGTTTGAGCTCATCATATGTTTATGGTTGGGTTGGATGTTAAGACGGCTGTTTTTTTTAGTTCTGTGACCAT * 7760 * 7780 * 7800 * 7820 * 7840 * 7860 * 7880 * 7900 * HtaiQT3-mt : GGTTATAGGAGTCCCCACAGGTATAAAGGTTTTTTCTTGGCTGTATATGTTGGCGGGAAGTCGGGGCCGGTTTTGGGATCCGATAATGTGGTGGATATTGGGTTTTATTGTCTTATTTACTATCGGGGGGGTGACCGGGATTGTGTTGTCTTCTTCTATAATGGATACTTTGTTGCATGA * 7940 * 7960 * 7980 * 8000 * 8020 * 8040 * 8060 * 8080 * HtaiQT3-mt : CACTTGATTTGTAATAGCTCATTTTCATTATGTTTTATCGTTAGGTTCTTATATGTCGGTGGTGATATCTATGATTTGATGATGGCCTGTGATAGTGGGTTATAGGTTGAAAAAGTACTTGTTGATGGCTCATTGGGGGGTGTCAATGGTTGGATTTAATTTGTGTTTTTTTCCTATGCA * 8120 * 8140 * 8160 * 8180 * 8200 * 8220 * 8240 * 8260 * HtaiQT3-mt : TTTTTTAGGGTTTCAGGGTTTACCTCGTCGGGTGTGTGTTTATGAGCCTTGTTTTCAGTGGTTGAAAACTATGGCTAGGCTGGGGGGACTTCTTTCTGTTTTTAGGGGGTTTATGTTAGCTTTCATTTTATGAGAGTCGTTGTCTGTGGGTAATCGGGTGATAGGGTTATGGGGTAGTAA trnT(Thr)(64) 7200 : 7380 7560 : 7560 7740 : 7740 7920 : 7920 8100 : 8100 8280 : 8280 rrnL(980bp) * 8300 * 8320 * 8340 * 8360 * 8380 * 8400 * 8420 * 8440 * 8460 HtaiQT3-mt : AGCCTTGGTTTTAAAAGTAGTAACTTCTCCTGTGCCGCATCATTGTAGTTATATGAGTGAGCCGGTAACGTGGTTTTATCGTTGGTAT TAG AGGGTGTATTTTAAGGAAGTAGAATGTAGCTTTTGTAAGGCTAAGGTGCTGGGGTCACCCTCT GGTTTATAGGCTGGTTAGGTGCTTAT * 8480 * 8500 * 8520 * 8540 * 8560 * 8580 * 8600 * 8620 * 8640 HtaiQT3-mt : TTCTGGGTTTATTTTACCTTTTGCATCATGATTCCTTGAGAGATTCCTTGTATAAGGGGTTCCGAAGTGCAGAGATTTCCTTTTGGTTAGCTTAGTGCTGTAGTTTTGGGCGGGTAGCCTTTGTTAAAGTCATTGGGGGAGGTGAAACATAAATCGTTCTGCTCGATATCTGATTTTTGC * 8660 * 8680 * 8700 * 8720 * 8740 * 8760 * 8780 * 8800 * 8820 HtaiQT3-mt : TAGGGTTTTCTTGGGATCCTTGTTGTTTGGAAATGATGGGGAGGGTAAGAGTCTTGCTGTGTTATTGATGGATTTAGGTTAGAGGTACCTTTTTGTTGTACTATGTGTTAAGACTGGGTAACTTAGCCTGTTGAGAGATACTTTGTTGGGGCTAGCAGAGGTTTAATTTTGTGTCGTGAG * 8840 * 8860 * 8880 * 8900 * 8920 * 8940 * 8960 * 8980 * 9000 HtaiQT3-mt : TATGAGGGAATAAGTAGTTTTATTAGCTCTCTCTTTTCTCGGGTGCCTCCGGTCTGTTTATTAAAAACATTTCCATTAGTAAGGATTAATGGTAGTACCTGCCCGATGTAATAATGAATGGCCGCAGTATCTTGACTGTGCTAAGGTAGCATAATTAGTTGCCTCGTAATTTGGGGATTG * 9020 * 9040 * 9060 * 9080 * 9100 * 9120 * 9140 * 9160 * 9180 HtaiQT3-mt : TTTGAATGGTTTGACTTGGGGGTTTATCTAGGTGGGGGCTTTGTTGCGAAATTGGTTGATCAGTGCAGAATCTGATCCTTTTTGATTAGACGGAAAGACCCCGAGAGCTTGCAGTAGGATGTTTTATCTGGGGAGGAGGCAAATGTTTCATTTGTTTTTAGACCCTTTTGGGTAAAAGAT * 9200 * 9220 * 9240 * 9260 * 9280 * 9300 * 9320 * 9340 * 9360 HtaiQT3-mt : TGAAGTTACCTCGGGGATAACTAGGTAAGAAGCAAGGAGAGGCCTTATTGATTTGCTTGGTTGCCATCTCGATGTTGACTTAGGATGTAGCGGAAGGGTGTAGCAGTTTTTCTGGTTGGTCTGTTCGACCTTAGAATCCTTCATGAGTTGAGTTAAGACCGGCGAGAGCCAGGTCGGTTC trnC(Cys)(64) HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : : 7200 7380 : 8460 : 8640 : 8820 : 9000 : 9180 : 9360 rrnS(748bp) * 9380 * 9400 * 9420 * 9440 * 9460 * 9480 * 9500 * 9520 * 9540 TTATCTATCTTTGTTGGAGGTTAGTACGAAAGGATTATCTCTAATTAATGTTTG GGTGTGATGCTAGTAAGGCAGGGTGCTCTGCAAAGGCACTTTAGATATTTTTATCCATACCT TATTTATTTAGCTTTGGTTATGGAAGTTTCTCGTGAGCGGTCTACATAGCTAAGTCTTTTGAGG : 9540 * 9560 * 9580 * 9600 * 9620 * 9640 * 9660 * 9680 * 9700 * 9720 AGCGATGGGGGTGTCGTGTCTGGTGCTAGTAGCGAGTTTTTGATGTTAGGTGGAAGCCTGATCAAGGGTTTATGAGATGTAAGTGGTGAATCCTCAGTGCCAGCATCCGCGGTTATTCTGTTTAGCTTGTACTTCTCTTAAGTTGAAATTCTTTATAAGTGAGTTAGGGTAGGGGGTAGA : 9720 * 9740 * 9760 * 9780 * 9800 * 9820 * 9840 * 9860 * 9880 * 9900 ATCTTTATCTGGTGGAGTTGTGCTTATAGTGGGTATAAGGGCTTTTTTGAAAAAAGGATTAGAGACCCTTGTATTGAGTAGAGTTATTTTGTGACTCTTTAGTTTAAACTAAAAAGGCTTGGCAGCTGACGAGATTCGTCCGGGGGAACGTGTGTCATAAGAGATGGTCCGCTTAATGGT : 9900 * 9920 * 9940 * 9960 * 9980 * 10000 * 10020 * 10040 * 10060 * 10080 TTACCAAGATTAGGGGTTAGTGTATATCCGGTTCAATATTCGTGGTTGGTGCCGCAGAGTGAATATAGGGGGGGTTATTAAAGCCAGGTCGATGTGCTGCTAATGACTTGGGGGATTATGCGTTACTGTTTAAAAGAGCCTTCTTCTAGAATGGAGGGGGGTAGTCAGGACTCGGAAGTA : 10080 cox2(624) * 10100 * 10120 * 10140 * 10160 * 10180 * 10200 * 10220 HtaiQT3-mt : GGTAGGTAATGTTTAGTCTTATCGAAGGGGGATTTAGTTGGGGTACACACCGCCCGTCACCCAAAGCGTTAGCTTTGGTAAGTCGTAACATGGTAATGCTGGGGGAACCGGGCGTTAGTTGGTGGATGGTGTATTTTATCCACTTG * 10240 * 10260 ATG GCTTCTGTTTTAGTCGGTAAAGAGATTTATG M ASVLVGNEIY * 10280 * 10300 * 10320 * 10340 * 10360 * 10380 * 10400 * 10420 * 10440 HtaiQT3-mt : AGGGACTTTTGTGGTATCTTGTGTTATTGGTGGCTTTTATTCCAGCTTGGGTATTTTTAGTTTTAGGTTGACAGATTACTGGTAAGTATAATCTTAGAATCGCTGCGAATGAGAAAAATGTCATAGAGTGGGCTTGGACGCTTGTACCTACTTTTATGGTTTTTTACCTGTGTTTTTTGA EGLLWYLVLLVAFIPAWVFLVLGWQITGKYNLSIAANENNVIEWAWTLVPTFMVFYLCFL * 10460 * 10480 * 10500 * 10520 * 10540 * 10560 * 10580 * 10600 * 10620 HtaiQT3-mt : ATTTGGGTTGTGTAGGGGAGGATATCCCGGTTCGGAGGGAAGGGTTAGTGAAGGTGACGGGTCGACAGTGACTTTGGAGTTATGAAATAAGAGGGGAGGAGAGGGGGTATGATTCAATAATGACGGATTTTGTCGATTCGGTGGATAAGCCTCTGCGGCTGGTTCTTAAAAATTATTATC NLGCVGEDIPVRSEGLVKVTGRQWLWSYEISGEESGYDSIMTDFVDSVDKPLRLVLNNYY * 10640 * 10660 * 10680 * 10700 * 10720 * 10740 * 10760 * 10780 * 10800 HtaiQT3-mt : ACCTCGTGGTAACTTCGTCGGATGTTATACATAGCTTTGCTCTACCGGAGTTGAATTTGAAGATGGATGCGATACCAGGGCGTTTAAATCAGGGGATAATGTATGCTGACCATTTGGGGTGTTTTGTGGGTTATTGTAGGGAGTTATGTGGTGCAGGACATGCGTATATGCCTATAGTCT HLVVTSSDVIHSFALPELNLKMDAIPGRLNQGIMYADHLGCFVGYCSELCGAGHAYMPIV * 10820 * HtaiQT3-mt : TGGAGGTGATTGCCCCTCGAAGGGGTATTGAGATGT : 10260 : 10440 : 10620 : 10800 nad6(489) (14 bp overlapping with 3’ end of cox2) LEVIAPRSGIEM 10840 ATGATAGCTGCTAG Y D S * 10860 * 10880 * 10900 * 10920 * 10940 * 10960 * 10980 GTTAGTTTTAAGGCTGTATTTTACTACGCTACTGTCTTTTTCTTTTATTACTCATCCGGTAGGGTATTGTTACTTATTACTATTAAGGGCTTTTAGGGTTATTGGTTATGTGTATTTGGTTTTGGGGTTT : 10980 C ASLVLSLYFTTLLSFSFITHPVGYCYLLLLSAFSVIGYVYLVLGF M I A * 11000 * 11020 * 11040 * 11060 * 11080 * 11100 * 11120 * 11140 * 11160 HtaiQT3-mt : AGATGATACTTGGTTTTGTTCTGTTTGGTTTATGTGGGAGGGGTTTATGTACTCTTTATTTTTGTTTCTATGCATAATCCAAATCCTTCGCCTATGCTGGGTGGGAGTTATGGGTTGGTATTGTTTGTTTATTTTTTTTTGGCTATAAATTTTGTTGGCTATTTTACAGGCTATGAGGGT : 11160 S W Y L V L F C L V Y V G G V Y V L F I F V S M H N P N P S P M L G G S Y G L V L F V Y F F L A I N F V G Y F T G Y E G trnY(Tyr)(63) * 11180 * 11200 * 11220 * 11240 * 11260 * 11280 * HtaiQT3-mt : TCTCTGGTGGAGTGTAGTCATTATCTCTGTAGGTATTTTGAGGGGATAACGTACTTGTTTTTTTGCACGATGTTGATGCTGGGTTTTGTGATAGTAAGAATAGTAGGGGGGGAGAAGTGTTCCTTTTATCGA TAG SLVECSHYLCSYFEGITYLFFCTMLMLGFVIVSIVGGEKCSFYR * trnL1(Leu1-CUN)(66) HtaiQT3-mt : * 11360 GATGATTGTTCAGTTGGGG trnS2(Ser2-UCN)(64)(3 bp overlapping with trnL1) * 11380 * 11400 * 11420 TTAGAGGTGCCAGAGTTTTATGGGGCTGGTTTAGGTCCAGTTAACGATATAAATTATCCCTTTGCG * * 11440 * 11460 * 11480 GGCAAGGGTTTGGCCGTTGATGATTTGAAGTCATTAGTTTTTCTTTGTTAGGAATGCTCGCTTAGCTTGG trnR(Arg)(64) HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : 11300 * 11320 * 11340 AACTAACTTAGTATAAGGAGTACATCGGTTTGTAGGTCCGAGGGT : 11340 trnL2(Leu-UUR)(68) 11500 * 11520 ACATGGATGCCAGAGGCGTTAATGG : 11520 nad5(1587) * 11540 * 11560 * 11580 * 11600 * 11620 * 11640 * 11660 * 11680 * 11700 G GTTGGTTTTAAGCATCAAATACGGGTGTTATTCCCTCCGTGTATTGGTCCCGTG TTTGATAACCATTGTGTGGGGTTGCGTTTCGGCCGCAATGAAGGGTTAGATGAACTCTATCAAAA GTG TTAAGGGCTGTTGTGGTGCTTTTTGTTGCGGTAATGCTTTTCTTGTTATCTATTCTT V LSAVVVLFVAVMLFLLSIL * 11720 * 11740 * 11760 * 11780 * 11800 * 11820 * 11840 * 11860 * 11880 GGTTATTGGGGGTGAGGGTGGTCTTTTTCGTGGTTAGGTTTTGTTTTTCGTGACTATGATTTTGTTTTTGTGGTAAGGGATGTGAGTTTGGTTTGTGAAGTAATGCTATTGTTCTGTGGTTCAGTGGCATTATTTTACTGTTTTCATTATTTTGGACAAGGCGCGGATGCTTTTTTTCTT GYWGWGWSFSWLGFVFRDYDFVFVVSDVSLVCEVMLLFCGSVALFYCFHYFGQGADAFFL * 11900 * 11920 * 11940 * 11960 * 11980 * 12000 * 12020 * 12040 * 12060 TATCCATTGATGGCCTGGTTCTTAGGGGTCATGTCTATTCTGGTAATGAGAGGTAGGGTGCTCTTGTCTCTCTTGATGTGGGAATACTTAGGCTTGGTGAGGTTTTTTTTAATCTTATTTTATTCTAACATGGCAAGTCTTCGGGCTTCGTTAATAACTCTTTTTGTCTCACGTTTTGGG YPLMAWFLGVMSILVMSGSVLLSLLMWEYLGLVSFFLILFYSNMASLRASLITLFVSRFG * 12080 * 12100 * 12120 * 12140 * 12160 * 12180 * 12200 * 12220 * 12240 GATGCCGCCTTATTTATGGTGGTTATGTGGGTTAGATGGTGGTTTGGGGCTTCGTCGATTTTATTGGGATTGCTGCTATTTTTTATTGTTTTAACTAAGAGGGCTTGTTACCCGTTTATTTCGTGGTTACTGGAAGCTATGCGAGCTCCTACGCCAGTAAGTTCACTTGTGCATTCTTCT DAALFMVVMWVSWWFGASSILLGLLLFFIVLTKSACYPFISWLLEAMRAPTPVSSLVHSS * 12260 * 12280 * 12300 * 12320 * 12340 * 12360 * 12380 * 12400 * 12420 ACTTTGGTGGCCGCGGGAGTTTGATATCTTTTTTGTTATGGGGATATGTTTAGGTACGGAATGTTAAGGGTATTGTTTATGTTGAGGCTAGTTACTGTTTTAGTAAGGGGGTTTTGTGCTTTGGTATTTAAAGATTTGAAGAAGTTGGTGGCCCTTTCTACTTGTAAAAAAGTTTCTTGA TLVAAGVWYLFCYGDMFSYGMLSVLFMLSLVTVLVSGFCALVFNDLKKLVALSTCNNVSW * 12440 * 12460 * 12480 * 12500 * 12520 * 12540 * 12560 * 12580 * 12600 TGTATACTGTTTTATGTTTGTGGAAGTCTGGATTTAGCTTTATTACAGTTGATAACACATGGGGTCTGTAAGTGTTACCTCTTTATGTCTATTGGGGACCTGATGGGCTTATCGGGGGGTAGGCAGAGTTCTGTGGGGGTTTATTTGTCCCGTTATGTGGGTCGGTTTTCAGTGTATTTA CILFYVCGSLDLALLQLITHGVCKCYLFMSIGDLMGLSGGSQSSVGVYLSRYVGRFSVYL * 12620 * 12640 * 12660 * 12680 * 12700 * 12720 * 12740 * 12760 * 12780 CAAAGCATTTTGGTGATATCCTTGAGAGGCCTCCCCTTCTTAGGGGTGTTTTTTAGGAAGCATATGTTGGTTGGCTCGGTTTGTTATGTTTATGGGTGAGGGTATTTTGGCTTAATGTTTTTGGGATTTTTTCTTTCTTATGCTTACTGTGTGCGGTTGGTATTGTTGCTTGTTAAGGGC QSILVISLSGLPFLGVFFSKHMLVGSVCYVYGWGYFGLMFLGFFLSYAYCVRLVLLLVKG * 12800 * 12820 * 12840 * 12860 * 12880 * 12900 * 12920 * 12940 * 12960 CTGGGAGGATTAAGGAGTGGTTATTCGAGTTCCTTTATTATTATTTCTGGTCTGAGCGTAGCGGGGACCTTGTTAAAATCGTGTGGAATGGGTGTGTTAGAAGAGAGTGCGGGATTACTTGTTAGTTATAGCTTTTTTGTAGGAATGATTCAGGTCTTGGGTTGTTTGGGAGGCTTCCTT LGGLSSGYSSSFIIISGLSVAGTLLNSCGMGVLEESAGLLVSYSFFVGMIQVLGCLGGFL * 12980 * 13000 * 13020 * 13040 * 13060 * 13080 * 13100 * 13120 * 13140 TTTTTTGTCGCCGGTGGGGAGGGGGTCCTTTTTGGGCCTTCTACTTTGAGTTATTCTGATAGGGTAGTTAGGGGGGTGTATGATTTTTATCTTCGTCTCAGTGCTCCTGCTGTTGTGTCTTTTTATCGTTGGGAGTATTATGTTTTTTCTTTGTGGCCACGGTTGACATCTAGTTTTCTC FFVAGGEGVLFGPSTLSYSDSVVSGVYDFYLRLSAPAVVSFYRWEYYVFSLWPRLTSSFL trnE(Glu)(73 * 13160 * 13180 * 13200 * 13220 HtaiQT3-mt : CTTTTTCGGCTTTCTGCCTTTTCTTTTAAATCCTTGGTTTTGTTTTTGTTGTGTTTTTTATTCTTATACTTTTTAGTCGGTGTT LFRLSAFSFNSLVLFLLCFLFLYFLVGV Long Int seq (246)(underlined) : 11700 : 11880 : 12060 : 12240 : 12420 : 12600 : 12780 : 12960 : 13140 ) * 13240 * 13260 * 13280 * 13300 * 13320 TAAATTACGTTTGA GTTGATTGGGTGTATATGCATTCTAAATTTTCGTTTTAGAGGAGGCCTTTTATTGGGGAGGGGCTAATCAATA ATTTTAAAT : 13320 * * 13340 * 13360 * 13380 * 13400 * 13420 * 13440 * 13460 * 13480 * HtaiQT3-mt : TTTTAGTTTGATTTTTTTTAATTCTGTCTGAAAGATGCTTGTTTTTTTTATGGGGTGGTTGCCCTAGTTTATCTCTCTGTTGTGAAAACTGTAAGGGGGGGGTATTTTATGTTTTTAGACGTTGTGTTTTATTAGGGAGGTTTTGTGTGTAGAGGGTATAAATTTTATTTTGATAGATTT 13500 : 13500 TRU1 (182) * 13520 * 13540 * 13560 HtaiQT3-mt : ATTGTGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAGAAAGGAAATAGGGAGGGG * 13580 * 13600 * 13620 * 13640 * 13660 * 13680 TAATATCTATATTATTATACCTTTAGTTCTAAAAAAAGAAAAGGGAATAAAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGAAAGGGTGATTGTCTCGATGCACTGGGGTTA : 13680 TRU2 (182) * 13700 * 13720 * 13740 * 13760 * 13780 * 13800 * 13820 * 13840 * 13860 GTTGTTTGATTGGAAGAGATAAATAGAAAAAAGGAATAGGGAGGGGGGGGACCCCCCCCTAATATCTATTTTTTTATACCTTTAGTTCCAAAAGAAAAAGAGGGATAAGAATATTGCAAAGGGTAAGAAAAGGTTTGTTCCCGGGCACCGAAAGGGAGATTGTCTCGATACACTGGGGTT : 13860 HtaiQT3-mt : Long Int seq (487) (underlined) * 13880 * 13900 * 13920 * 13940 * 13960 * 13980 * 14000 * 14020 * 14040 TGTTTTTGATTGGCAGAGATTAATAGAAAAAAGGAATTGGGAGGGGGGGGGACCCCCCCCC TAATATCTATATTATCATACCATTAGTTCTAAGAGAAAGAAGGAATAGAAATTGGCTAGGATAGATAGGTTTGATGCTTGTGCGCAGAGGGGAATGTCTCAACCACTGGGTTATTGTTG : 14040 * 14060 * 14080 * 14100 * 14120 * 14140 * 14160 * 14180 * 14200 * 14220 HtaiQT3-mt : TCGCAGATATAGAGAAGGATTGGAGGGGGCACCCCCGAATCATATTACACCTTGGTCAATAAAAAGGAATAAAAATTGCAGGAAGAATCGGACCTACGCTAATGTGGATTCCGACCTGTTATGTTCTTAAGCCATGAGAGGACCTTGTGGGTGACCCCTTTTTTCTTTTTAGCGGTGTTC : 14220 * 14240 * 14260 * 14280 * 14300 * 14320 * 14340 * 14360 * 14380 * 14400 HtaiQT3-mt : TAAGAGATGAGGGATGACACTCGCAGGATGGTATGTTTGAGCCTCTCAGTGGAAGGGGGATTCCCCCACCACCCTGTTTAGTTTTTGATTGGAGAGACTATTTAGAAGAAAGGCAGTTGTGAGGGGGGGACCCCCCCCCTTTTTTTTTTTTTTTTCTTGCCCTTATTTTTAAAAGGAAAG : 14400 HtaiQT3-mt : TRU3 (183) * HtaiQT3-mt : AGGGGGGG * HtaiQT3-mt : * HtaiQT3-mt : 14420 * 14440 * 14460 * 14480 * 14500 * 14520 * 14540 * 14560 * 14580 TAGAATATCTGGCAAGAGATTGGATAGGTTTGAAGCCTCTGCCGCGGAAAGGGGGATTTCCCGATGCACTGTGGTTAGTTGTTTGATTGGGAGAGGTTTATTGGAGAAAACGATTTTGGGAGGGGGGGGGACCCCCCCCCTTATATCTTTTTTTTCATACCCCTATTTTTAA : 14580 TRU4 (183) 14600 * 14620 * 14640 * 14660 * 14680 * 14700 * 14720 * 14740 * 14760 AAGAAGAAGAGGGGATAGAATATTGGCAAAGGATTGGATAGGTTTGAAGCCTGTGCAGCGGAAGGGTGATTTTCTCGATGCACTGTGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAGAAAGGAAATTGGGAGGGGGGGGACCCCCCCCTAATATCTATATTATCATACCATTAGTTC : 14760 14780 TRU5 (183) * 14800 * 14820 * 14840 * 14860 * 14880 * 14900 * 14920 * 14940 TAAAAGAAGAAGAGGGAATAGAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGGAAGGGTGATTGTCTCGATGCACTGTGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAGAAAGGAAATAGGGAGGGGGGGGACCCCCCCCTAATATCTATATTATCATACCATTAG : 14940 Unique Seq (underlined) trnG(68) HtaiQT3-mt : * 14960 * 14980 * 15000 TTCTAAAAGAAGAAGAGGGAATAGAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCA * 15020 * 15040 * 15060 * GCGGAGGTAGTATAGGTTTGAGTACGCTGTCTTTCCAAGTCAGATGTCCCGTTGATCGGGTCTCTGCA 15080 * 15100 * 15120 CCTTACTCTTAGTTTACTCTGTTTGAGGCGGGTCTGAATGTGGGGGATG : 15120 nh nh i n gi i g n ùng gi o g n củ mtDNA hoàn ch nh ( oài Haplorchis taichui củ (Htai-LA/GenBank: MG972809) p) (645) cox3 * * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 180 AGGTGGTTACCTGTTTATAATTCTTGAGTGGTTATGGTGGGTATAGTAAGTTTGTTTTTGTGAAAGCTTTGGGGCGTTTTTTTCTTTTTGGGTGCGTTGATTTTTTCGGCTTATTTTCTTGTATGAGAGGTTTTTTTTCCACATTTAAGGCGGTATGCTATGGCTTTTTGACTATTT : 180 * * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 360 Htai-LA(15 : ATAGTTAGTGAGGTGGCTATTTTTGGGAGGTTGCTTTTAACTGTTCTTTGAAAAGATGACGGTGGGGAGGGGGCCTTGTCGGATAGCTTGGAGTTGCCTTTTTTAGGGTGTTTTCTTTTGTTGACTTCCTCTTTGACGGCCACGGTGTATCATCATTGTTTTGGCTTGCCTTATAGTTGG : 360 * * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 540 Htai-LA(15 : GTTTTTTTACTTATCAGTATCTTTTTAGGGTTTGGGTTTGTGTGTTTGCAGTTGTGGGAGTTTTTTGATTGTGAGTGTGATGTTCTTTATTGTACTTATTTTGGGGCTGCTTTCTGTACGGTTGGGTTGCATTTTAGTCATGTGGTTGCGGGGGTCGGTGCTATGACCGTTTTGTTAGTG : 540 * * 560 * 580 * 600 * 620 * 640 * trnH(66) * 680 * 700 cob (1110) Htai-LA(15 : AGGGGTTGGGATACCAATCATTTTTATGCTAGGGTGGTTGTGTGGTATTGGCATTCTGTTGATTATGTGTGGTTGTGAGTATACCTGTTAATATATTACGTT TAGGG TCTTCAATAGGTTATTTAAAACTGTTGTCTTGTGGAGACAGAGATTCGTTGTGTGTGATTGAGGAA GGTTTA A : 720 * * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 900 Htai-LA(15 : TG GTTTCTGTTGTTCGTGGTAAAGTAGTGGATCTTCCTACTAAAGTTTCTTTGAGGTATTTTTGATGTGGGGGGTTTATGATTAGTGTGTTTTTAGTCATTCAGGTTGTATCTGGTGTAATTCTGTCTTTGCTTTATGTAGGAGATGCGGGGGTGAGGTTCGGGTTGGTGCTGGATTTTG : 900 * * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 1080 Htai-LA(15 : TTAACGAGAGTTTGTTTTTATGATTTGTCCGTTATTTGCATATATGGGGAGTAACGTTTATTTTCCTTCTGTTCTTGGTTCACATGGGGCGTTCTTTTTATTATTCTAGTTATAGGAAGTTGGGGGTATGAAAAGTTGGGTTCGTTCTTTATATTTTAATGATGGTTGAGGCGTTTTTAG : 1080 * * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 1260 Htai-LA(15 : GGTATATTCTTCCGTGGCATCAGATGTCTTATTGAGCGGCCACTGTTTTAACCTCGATACTTAAAAGTATACCAGTTGTAGGCGGTAAGCTTTATGAGTTTATTGTTGGGGGCTTTGGGGTCACTAAAGTGACTCTAGTCCGTGTTTTTTCAGCTCATGTGTGCCTGGCTTTTGTGATAA : 1260 * * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 1440 Htai-LA(15 : TTGGGCTGAGTGTGATTCATCTTTTTTATTTGCATAAGGGAGGGTCTAATAATCCTTTATCTGCGCCTGGGGGCTACAGGGACGTTGTCCTGTTTCATTCATATTTCACTAGAAAGGATGGGGCGGTGCTTGTATTATTGTTATTACTCCTGGGAGGAGGGTTGATGATATCGCCAGACG : 1440 * * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 1620 Htai-LA(15 : CTGTTCTAGATGTGGAAAGGTATATTGAGGCGGATCCGATGGTTACTCCTGTGTCAATAAAGCCTGAGTGGTATTTTCTTGCTTTTTATGCGATGCTGCGGTCTATAGAGTCGAAGGTTGGGGGCTTGGTGCTGGTTTTGATATTCCTGTTCGTTTTGTGATTACCTACTTTTAATTCCT : 1620 * * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 1800 Htai-LA(15 : CTTGTGTTTATAGTGTGTTGCGTCAGTATGTATTTTGGGGCTGTTGTTCTCTTTACTTCATTTTGAGCTATCTTGGTGCTTGTCACCCCGAATATCCTTTTGTTTTTATAAGCAAGTTTTGTTCTCTCTTGATTGTTGTATTATTAATGCTGTTTAAGGGGCTGTGGGCAGTTCCTTATT : 1800 Htai-LA(15 : ATG nad4L * 1820 Htai-LA(15 : CTGGGGGGTTTCCTCGTTTGGTTTTG TAG * T ATG (264) * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 1980 GTTCGGTTGCTTTTGCTTTTAGGCATTTTTATAATTTTGTGTAGGTTTTTTCTTTCTTTGACGCGCTTGTTGAAATGCCTAATAGTGGTTGAGAATTTTAAAGTGTTGGTGTTATTTGTGAGTTTATTATCCCAGCGGGGTGAGACT : nad4 * 2000 * 2020 * 2040 * Htai-LA(15 : TTTATACTTTTCATTGCCTTGATGGTGATTTTTACTATTGAGGTTATTCTTGGTTTGGTGGTTTTGACCCGGTT * 2180 * 2200 * 2220 * (1281) 2060 * 2080 * GTGGGATTCAAGAGGTTTAGTTGGTATAGTGGGTTGATAG 2240 * 2260 * * 2100 * 2120 * 2140 2160 GGGCCTTGTCGGTTAGTGGTTTGATTATTTTGGTTGAGGCCTGTGCAGGGTTGGATAGAATCTGGC : 2280 * 2300 * * 2320 1980 2160 2340 Htai-LA(15 : TTAGTCATTCTGGGGTTACGGCGGGAGTTTTTTGTTTTGATGTTATAAGCTTTTATCTGATTCTGCTTTCAATTTTATTATCCTTGTCTTTAAGTTTTTGGGTTGAGAGTATGAGATATTTTAGACGCTTTATGATTTTCTTGAGAGTGCTAGGGTCGCTTTTATGTTACTGTTGTGTTC : 2340 * * 2360 * 2380 * 2400 * 2420 * 2440 * 2460 * 2480 * 2500 2520 Htai-LA(15 : ATGTCGTTTGGTTTTGAGTTTTTTATGAGATGTCAATAGTTCCGTTGCTTGTGTTGTTAATTGTAGATTCTCCTTATTCGGAGCGTTATGTTGCTTCTTGATATCTTTTGGGGTATGTGGTTTTTACGAGTCTCCCAATGTTGCTGTGTATACTTTATTTGTCTTTACTTGAGGGGGGGT : 2520 * * 2540 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660 * 2680 2700 Htai-LA(15 : TTAATATGGGGTGTTGGGGGTCTTCTGATAGGTGGGTTGTAACCGGGAGGTTAATTGTTTTAGCCTTTCTTTTTATTACAAAGATACCTTTGCCTCCGTTTCATGTGTGGCTTCCGATAGTTCATGCGGAGGCTAGGAGCCCAGTATcTGTCTGTTTGAGGGGGTATATAATGAAGTTGG : 2700 * * 2720 * 2740 * 2760 * 2780 * 2800 * 2820 * 2840 * 2860 2880 Htai-LA(15 : GTTTACTTGGTGTCTGCCGGTTTTGTTTTTTTTTACTTCCCGATTTTATTTTCTCTTTGGCTTATACGGTTATATGCTTTTTATGTTCTGTGCTCTTCTTCTTTTCTGCTAGTCGGGAGTTGGATGGTAAGCGCTGACTTGCTTTTTTAAGTCTTTCACACATTTTAGTGGCTATGGGCT : 2880 * * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 3060 Htai-LA(15 : GTCTGTCTGTGGGGGGTTGAGTATTGAGGGGCTCAGCTATTTTGTATTGTATGGGTCATGGGCTGTCTGCGGGGGTGATGTTTTTATTTCTTTGGTTGATGTATGATGTGGTGGCTACTCGTAATTTAGCTTTGTTGAAGTCGGGGATTTCGGGAAGTTTGTTGTTTCGTGTGTTGGCTT : 3060 * * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 3240 Htai-LA(15 : GTTGCTGTTTATGTACTGCTTGTTCTTTGCCCCCCACAGTACAATTTTTTTGTGAAGTTTCGTTAATGACTGATGGGGCCTGGAGGGGGGGTTTCTTTTTACTAGTTTTTTATGCTTATGTCTTTATGGGAGGCTTGATGCCTTTATTTTTAGTAGGAAGGTTATTGAGCCGGCATTATG : 3240 * * 3260 * 3280 * 3300 * 3320 * 3340 * trnQ(66) * 3380 * 3400 trnF (64) Htai-LA(15 : ATGTGAGGTTTCATGGGGGTTCAGCAATCGGTGTAAAGGTTTATTCTTTATTCTACTTGATTATGTGGAGGTTTGGCTTATTTCTGGTATGT TAA ATTGGGGTT TATAGCTTGGTGTAGTTAGAAGCATGCTATGTTTTGGTTGTAGCGGGGATTGGATGTTAGCTATAG TGT TTCCGCC : 3420 atp6 (516) * * 3440 * 3460 * 3480 trnM (64) * 3500 * 3520 * 3540 * 3560 * 3580 3600 Htai-LA(15 : TAGCTTAAATTTAAAGCGTTAGTTTGAAGAGCTGAAGATATTATATTGTGGTGGGA G GCGGGATAAGTTAAGTGAGAAACTCTATGGTTCATGTTCATAAAATGCGTGTAGCTCCTGCCT ATG TGTTTTTCCCGTGTGAGAAGAGTTTATAATAATGTGATTAGATATGTGGCGGGGGGA : 3600 * * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 3780 Htai-LA(15 : TTGGGAGATAGATTTTATCGTTTACTTCTTTTTATGGTGTTGTTAGTTTTTTTGGGGTTACGGCTTCCTTATTTTTATGGGATGGGGGGATTCGGTATTTTTATTTTGATTAGGGTTTTCCCCCTTTTTATGAGTTTGTTTTTTTCTCGTTTGATTGATGGTAGTATGAGTGCGTTTTTT : 3780 * * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 3960 Htai-LA(15 : TGTGGCTTTGTTCCTCAGGGTACTCCGTTGTGGATAGCTCCGTTTGTTTGTTTGGCTGAAACTCTAAGTTATGTTGTGCGGCCCGTGGTTTTGATGATACGTCCTTTTGTAAATCTGAGTATTGGAGCTTTAGGGGGGGCAGTATTGGGTGCTTTAAGTATGACTAGAGGTTGAGTTTTA : 3960 nad2 (870) * * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 4140 Htai-LA(15 : TTTTTTTTGGTTCTTTTATTTTTTTATGAGGTCTTTGTCGCGTTGGTTCATTGATTTATCGTGTGCAGTATCCTTTCTTTTTCGGAGGATCAC TAG AAGGGGGGGGGGTAATCGGGCTTAG ATG CGCGGGCTGGCGTTATCTGGTTTTAGGGTGGTCAGTGTTTTCTTGTTCTCTTTATG * * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 4320 Htai-LA(15 : CTTGTTCTTGAGAGATAATCTTTTATTATTCTGGCTGTTTTTGGAACTTGGCGCTTTGAGGTTGGTTCCTGGATTTTTTATAGTTGGTGGGAGGGGCGCCTTGGGCGGTCTTTTTAGCTACATTTTGGTTTCTGCGGTTTCTTCTTCGTTAATAGTAAGGGGACTGTTATACGAGGATGT * * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 4500 Htai-LA(15 : TTTTTTTCTGTCTTGTTTGGGATTGATAATGAAGTTTGGGGTTTTCCCCTTTATGGGTTGAGTATATGTTGTTGTTTTGGCTAGCAACTGGTTAGTAATATGATTGCTTTCGACTGCTTTAAAGATGGGTTTTTTCTTTTTTTGTTTTTTTATTAGGAGGGGGTGAGATTCTTTGCTTGT * * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 4680 Htai-LA(15 : AATGATGTTGTGTGTGTTTACCTTTTTGGTGTTGAGTCTGTTGTTTTGGGTTTTTACTCCTAGTTGACTGGTTTATTGGTGTCATGTCTTGATTAGATCTAGGGCTTCTTTGATACTAATGGCTTTGGAGGTTTCAGTTGATTTACTTTTATGACTGTTTGTTATTTATTTTGTGTGGTC * * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 4860 Htai-LA(15 : TACTTGTGCTATTATGCTTTTACGTAGGCTTAGCTTGATGAGCCTGGGTAATAGCGTGGTGGTTTTTTGGGTCGTGTTTATTTTGGTGGCGTTTCCTTTCTCTGTTTCAATTTTTTATAAGTTGGTTGTTAGTCTTTGTATGTACTCGTGTGGTGTTTTAGCATTGTTGGGGTGGGTTGT * 4880 * 4900 * 4920 * 4940 * 4960 trnV (60) * 4980 * 5000 * 5020trnA (63) * 5040 Htai-LA(15 : GTATAAAGTTTCGGAGCAGTTTTTTTTAGTTAAGTATATGGTGAGAGAAGGTATTCCCCGTGCGGAGTGAAGAGGAGCAGGTGTGGTT TAA GGGA GGCGAGATAGTTTATTGAAAATGTCTATTTTACGCGTAGAAGAAGGATTACCTATCGTCA G AACGATATAGTTTAATAGGAGATG : 4140 : 4320 : 4500 : 4680 : 4860 : 5040 Htai-LA(15 : GCCGCTCTGCAAGCGGTCGGTGAAGGATTTCTATCGTTA GC ACTTGGTTGTTGGGCGTTTATAATTTTCTTAGGTCGTTTTTGGGGTTTGCGGTGATAATGATTTTTGTA : CTGTTCTTAGTTTATTGTAATAATAGCTTGTCGTGCTATAGGAGGGTGGCTAGACACTCAGAGCGGG GTG * * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 5400 Htai-LA(15 : GCCTTCTTTATTTTGGGGGAGCGCAAGGTTTTGGGTTATATGCAGATTCGTAAGGGTCCGAACAAGGTGGGGCTTGTAGGGCTTTTTCAAAGGTTTGCCGATTTGTTGAAGTTGATAATAAAGTTTAAGGTTAGCCTTTATCAGGTTCGTAGGTGACTCTCTTGAATTGGGGTTTATCTT : * * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 5580 Htai-LA(15 : TTGTTATTTTTGGCGCTTTGTTATTGTTTTGTTTTTGGGTTGTTTTATGGTGGTGTGAGTTGTGTAAAAGGTTTGCTTTGGGTATTGGTGGTAACGAGTATAACGGGGTATAGTCTTTTAAGCATTGGTTGAGGGTCTTATAAAAAGTATGCTTTGGTTAGTTGTGTCCGTTCTGCTTTT : * * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 5760 Htai-LA(15 : GGGTCGGTGACTTTTGAGGCGTGTTTTATGTGTATTGTGGTGATTGTGGGTGTAGTCTGTGGGGGTTACTTTGGTTACTCGGTGATGGAGAATGCGTGGTTAGTCTTGTTGGTGAGACCTTTAGGATATGCTTTATGATTAGTGGGGATGTTATGTGAGTGTAAACGAACTCCTCTAGAT : * * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 5940 Htai-LA(15 : TATGCGGAAGCTGAGAGGGAGCTGGTGAGAGGGTTGAATACGGAGTATTGTAAAGTGCCTTTTACTTGCTTGTTTGCTTGTGAGTATCTGATAATGTTTATTTTTTCTTGGTTGGGGGCTTTATTATTTTGGGGAGGGGGCCTAATTTCGTTTTTTAGGTTTGTTCATGTTTTGTTTTTT : * * 5960 * 5980 * 6000 * 6020 * 6040 * 6060trnN (66) * 6080 * 6100 6120 Htai-LA(15 : ATTTGGGCGCGTGCAACTTTACCTCGAGTGCGGTATGATTATTTTGTGGCTTTTATGTGAAAGTACTCAATCCTTATATTGGTTTTTTCGTTATTTTTAGTGGTGTTAGTT TAGTG GTGTGGGTAGATTAATGGTAAAATCGTTAAGCTGTTAACTTAAAGATGATCGGGTATCCTCAGA : * * trnP (65) * 6160 * 6180 *trnI (65) * 6220 * 6240 * 6260trnK (65) * 6280 6300 Htai-LA(15 : CG AGG CAGTCGTATAGTCTATTTTAGGATGTTAGCTTTGGGGGCTTAAGGACTCTTGAGGAGTGGGC TGATAGGGCTGCTGAGCAGGCCACTGTGATATAGTGGTTGTAGGAGAATTAGATCCTCGTCGGT AGA CTGTGGGTAGCTTAAGGTGTAAAGTGTTAGATTCTTACTCTAGTGA : 5220 nad1(906) * nad3 5060 * 5080 trnD (67)* 5100 * 5120 * 5140 * 5160 * 5180 * * 5200 5220 cox1 Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : rrnL 5580 5760 5940 6120 6300 (360) * * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 6480 Htai-LA(15 : TGTTTTTGAACTCCATAGG ATG GGTTATTCTGGCTTGTGTGCTCTTTTATTCTTTTTGATTTTTTTATTGGTCGGTGTATTCCATTTATTTTTATGGGATTCTGGTTTGTGAGGGTTTGTAAGTGGGTTTCGGTCTTGGGTCGGTAGGTTTGAGTGTGGTTTTATCTCTCAGGGGGTGTC : * * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 6660 Htai-LA(15 : AGAGAAATATTTTAGGTGAACTTACTTTATACTGCTAGTCTTTTTTGTTGTTTTTGACTTGGAAATTTCGTTACTTCTAAAATTACCTTTACAAGGGCTGCTTTTTAAGAAATTGCCCTATTATGTGTGTTTTGTGTTACTCCTGTGCTTTGGTTTTGGGATTGAGGTGAATAAGGGTTA : Htai-LA(15 : 5400 (1542) * * 6680 * 6700 trnS1 (62)* 6720 * 6740 * 6760 trnW (64)* 6780 * 6800 * 6820 6840 TGTCGAGTGAAGCTAC TAGGGGTTAGATGTGT GAGAGCTATGTGAAGCTGTTGCTGCTAACAATGGTAAGAGTGTTTAAAGCGCTCGGCTCTCTTGTAGGG AACAATCTAGGTTAAGTAGAGACTGTCTGTCTTCAAAACAGAGGGTAGCTTGCGCTGTTTGTTG TTAGATG TTGACTTG * * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 7020 ATTATTGACTTTGGATCATAAGCGAATAGGGTTGATATATATGATTGTTGGAGTGTGAGGGGGCTTTTTAGGTCTTTCTTTGAGGTTTTTGATCCGTGTTAACTACTTGGATCCTTACTATAATGTTGTATCCCCAGAGGTTTATAATTATGTAATTACTAGGCATGGTATTGCGATGAT * * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 7200 TTTTTTTTTCTTAATGCCGGTTTTGATTGGGGGGTTTGGAAAATATCTGTTGCCCCTGTTGTTGGGGCTTCCTGATTTGAATTTACCCCGTTTAAATGCCCTTAGAGCTTGGTTAATGATTCCTTCCGCTGTTTGTTTTGGGTTAAGTATGTTTGGGGGGTCTGGGGTTGGCTGGACTTT * * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 * 7340 * 7360 7380 TTATCCCCCCCTGTCTTCTAGGGATTATAGGGGTTGGGGTGTTGACTTTCTTATGTTTTCTTTACATTTGGCGGGGTTATCTAGTCTGTTTGGTTCTTTAAACTTTATTTGTACAATAATGAGGGCTATGAGTAATGATACCAGGGAGCGGTACTCTATTGTGGTTTGGGCTTATTTGTT * * 7400 * 7420 * 7440 * 7460 * 7480 * 7500 * 7520 * 7540 7560 TACTTCTATTCTTTTGTTGCTTTCGTTACCAGTGCTAGCTGCGGGTATAACGATGCTTTTGTTTGATCGTAAATTTAGCTCTTCTTTTTTTGATCCATTGGGAGGGGGGGACCCTGTCTTGTTCCAGCATATGTTTTGGTTTTTTGGGCACCCCGAAGTTTACGTTCTTATCCTCCCAGG * * 7580 * 7600 * 7620 * 7640 * 7660 * 7680 * 7700 * 7720 7740 GTTTGGTATAGTGAGACACATTTGTAGGACGTTAACAAATAAAGATTCCTTGTTTGGTTATGGGGGTTTAGTTCTTGCTATGTTTTCTATAGTCTGTTTGGGGAGTGTTGTTTGAGCTCATCATATGTTTATGGTTGGGTTGGATGTTAAGACGGCTGTTTTTTTTAGTTCTGTGACCAT * * 7760 * 7780 * 7800 * 7820 * 7840 * 7860 * 7880 * 7900 7920 GGTTATAGGAGTCCCCACAGGTATAAAGGTTTTTTCTTGGCTGTATATGTTGGCGGGAAGTCGGGGCCGGTTTTGGGATCCGATAATGTGGTGGATATTGGGTTTTATTGTCTTATTTACTATCGGGGGGGTGACCGGGATTGTGTTGTCTTCTTCTATAATGGATACTTTGTTGCATGA : * * 7940 * 7960 * 7980 * 8000 * 8020 * 8040 * 8060 * 8080 8100 CACTTGATTTGTAATAGCTCACTTTCATTATGTTTTATCGTTAGGTTCTTATAGGTCGGTGGTGATATCTATGATTTGATGATGGCCTGTGATAGTGGGTTATAGGTTGAAAAAGTACTTGTTGATGGCTCATTGGGGGGTGTCAATGGTTGGATTCAATTTGTGTTTTTTTCCTATGCA * * 8120 * 8140 * 8160 * 8180 * 8200 * 8220 * 8240 * 8260 8280 TTTTTTAGGGTTTCAGGGTTTACCTCGTCGGGTGTGTGTTTATGAGCCTTGTTTTCAGTGGTTGAAAACTATGGCTAGGTTGGGGGGACTTCTTTCTGTTTTTAGGGGGTTTATGTTAGCTTTCATTTTATGAGAGTCGTTGTCTGTGGGTAATCGGGTGATAGGGTTATGGGGTAGTAA : (980) 6480 6660 : 6840 : 7020 : 7200 : 7380 : 7560 : 7740 7920 : 8100 8280 * * 8300 * 8320 * 8340 * 8360 * trnT(64) * 8400 * 8420 * 8440 8460 Htai-LA(15 : AGCCTTGGTTTTAAAAGTAGTAACTTCTCCTGTgCCGCATCATTGTAGTTATATGAGTGAGCCGGTAACGTGGTTTTATCGTTGGTAT TA GGTTTATAGGCTGGTTAGGTGCTTAT : 8460 GAGGGTGTATTTTAAGGAAGTAGAATGTAGCTTTTGTAAGGCTAAGGTGCTGGGGTCACCCTCT * * 8480 * 8500 * 8520 * 8540 * 8560 * 8580 * 8600 * 8620 8640 Htai-LA(15 : TTCTGGGTTTATTTTACCTTTTGCATCATGATTCCTTGAGAGATTCCTTGTATAAGGGGTTCCGAAGTGCAGAGATTTCCTTTTGGTTAGCTTAGTGCTGTAGTTTTGGGCGGGTAGCCTTTGTTAAAGTCATTGGGGGAGGTGAAACATAAATCGTTCTGCTCGATATCTGATTTTTGC : 8640 * * 8660 * 8680 * 8700 * 8720 * 8740 * 8760 * 8780 * 8800 8820 Htai-LA(15 : TAGGGTTTTCTTGGGATCCTTGTTGTTTGGAAATGATGGGGAGGGTAAGAGTCTTGCTGTGTTATTGATGGATTTAGGTTAGAGGTACCTTTTTGTTGTACTATGTGTTAAGACTGGGTAACTTAGACTGTTGAGAGATACTTTGTTGGGGCTAGCAGAGGTTTAATTTTGTGTCGTGAG : 8820 * * 8840 * 8860 * 8880 * 8900 * 8920 * 8940 * 8960 * 8980 9000 Htai-LA(15 : TATGAGGGAATAAGTAGTTTTATTAGCTCTCTCTTTTCTCGGGTGCCTCCGGTCTGTTTATTAAAAACATTTCCATTAGTAAGGATTAATGGTAGTACCTGCCCGATGTAATAATGAATGGCCGCAGTATCTTGACTGTGCTAAGGTAGCATAATTAGTTGCCTCGTAATTTGGGGATTG : 9000 * * 9020 * 9040 * 9060 * 9080 * 9100 * 9120 * 9140 * 9160 9180 Htai-LA(15 : TTTGAATGGTTTGACTTGGGGGTTTATCTAGGTGGGGGCTTTGTTGCGAAATTGGTTGATCAGTGCAGAATCTGATCCTTTTTGATTAGACGGAAAGACCCCGAGAGCTTGCAGTAGGATGTTTTATCTGGGGAGGAGGCAAATGTTTCATTTGTTTTTAGACCCTTTTGGGTAAAAGAT : 9180 * * 9200 * 9220 * 9240 * 9260 * 9280 * 9300 * 9320 * 9340 9360 Htai-LA(15 : TGAAGTTACCTCGGGGATAACTAGGTAAGAAGCAAGGAGAGGCCTTATTGATTTGCTTGGTTGCCATCTCGATGTTGACTTAGGATGTAGCGGAAGGGTGTAGCAGTTTTTCTGGTTGGTCCGTTCGACCTTAGAATCCTTCATGAGTTGAGTTAAGACCGGCGAGAGCCAGGTCGGTTC : 9360 rrnS (748) * 9380 * 9400 * 9420trnC (62) * 9440 * 9460 * 9480 * 9500 * 9520 * 9540 Htai-LA(15 : TTATCTATCTTTGTTGGAGGTTAGTACGAAAGGATTATCTCTAATTAATGTTTG GGTGCGATGCTAGTAAGGCAGGGTGCTCTGCAAAGGCACTTTAGATATTTTTATCCATACCT TATTTATTTAGCTTTGGTTATGGAAGTTTCTCGTGAGCGGTCTACATAGCTAAGTCTTTTGAGG : 9540 * 9560 * 9580 * 9600 * 9620 * 9640 * 9660 * 9680 * 9700 * 9720 Htai-LA(15 : AGCGATGGGGGTGTCGTGTCTGGTGCTAGTAGTGAGTTTTTGATGTTAGGTGGAAGCCTGATCAAGGGTTTATGAGATGTAAGTGGTGAATCCTCAGTGCCAGCATCCGCGGTTATTCTGTTTAGCTTGTACTTCTCTTAAGTTGAAATTCTTTATAAGTGAGTTAGGGTAGGGGGTAGA : 9720 * 9740 * 9760 * 9780 * 9800 * 9820 * 9840 * 9860 * 9880 * 9900 Htai-LA(15 : ATCTTTATCTGGTGGAGTTGTGCTTATAGTGGGTATAAGGGCTTTTTTGAAAAAAGGATTAGAGACCCTTGTATTGAGTAGAGTTATTTTGTGACTCTTTAGTTTAAACTAAAAAGGCTTGGCAGCTGACGAGATTCGTCCGGGGGAACGTGTGTCATAAGAGATGGTCCGCTTAATGGT : 9900 * 9920 * 9940 * 9960 * 9980 * 10000 * 10020 * 10040 * 10060 * 10080 Htai-LA(15 : TTACCAAGATTAGGGGTTAGTGTATATCCGGTTCAATATTCGTGGTTGGTGCCGCAGAGTGAATATAGGGGGTTATTAAAGCCAGGTCGATGTGCTGCTAATGACTTGGGGGATTATGCGTTACTGTTTAAAAGAGCCTTCTCCTAGAATGGAGGGGGGTAGTCAGGACTCGGAAGTAGG : 10080 cox2 * 10100 * 10120 * 10140 * 10160 * 10180 * 10200 * 10220 * 10240 * 10260 Htai-LA(15 : TAGGTAATGTTTAGTCTTATCGAAGGGGGATTTAGTTGGGGTACACACCGCCCGTCACCCAAAGCGTTAGCTTTGGTAAGTCGTAACATGGTAATGCTGGGGGAACCGGGCGTTAGTTGGTGGATGGTGTATTTTATCTACTTG ATG GCTTCTGTTTTAGTCGGTAAAGAGATTTATGAG : 10260 * 10280 * 10300 * 10320 * 10340 * 10360 * 10380 * 10400 * 10420 * 10440 Htai-LA(15 : GGACTTTTGTGGTATCTTGTGTTATTGGTGGCTTTTATTCCAGCTTGGGTATTTTTAGTTTTAGGTTGACAGATTACTGGTAAGTATAATCTTAGAATCGCTGCGAATGAGAAAAATGTCATAGAGTGGGCTTGGACTCTTGTACCTACTTTTATGGTTTTTTACCTGTGTTTTTTGAAT : 10440 * 10460 * 10480 * 10500 * 10520 * 10540 * 10560 * 10580 * 10600 * 10620 Htai-LA(15 : TTGGGTTGTGTAGGGGAGGATATCCCGGTTCGGAGGGAAGGGGTAGTGAAGGTGACGGGTCGACAGTGACTTTGGAGTTATGAAATAAGAGGGGAGGAGAGGGGGTATGATTCAATAATGACGGATTTTGTCGATTCGGTGGATAAGCCTCTGCGGCTGGTTCTTAAAAATTATTATCAC : 10620 * 10640 * 10660 * 10680 * 10700 * 10720 * 10740 * 10760 * 10780 * 10800 Htai-LA(15 : CTCGTGGTAACTTCGTCGGATGTTATACATAGCTTTGCTCTACCGGAGTTGAATTTGAAGATGGATGCGATACCAGGGCGTTTAAATCAGAGGATAATGTATGCTGACCATTTGGGGTGTTTTGTGGGTTATTGTAGGGAGTTATGTGGTGCAGGACATGCGTATATGCCTATAGTCTTG : 10800 nad6 (14 bp overlapping with 3’ end of cox2) * 10820 * 10840 Htai-LA(15 : GAGGTGATTGCCCCTCGAAGGGGTATTGAGATGT ATG ATAGCTGCTAG * 11000 * 11020 * * 10860 * 10880 * 10900 * 10920 10940 * 10960 * 10980 GTTAGTTTTAAGGCTGTATTTTACTACGCTACTGTCTTTTTCTTTTATTACTCATCCGGTAGGGTATTGTTACTTATTGCTATTAAGGGCTTTTAGGGTTATTGGTTATGTGTATTTGGTTTTGGGGTTTAG : 10980 * 11040 * 11060 * 11080 * 11100 * 11120 * 11140 * 11160 Htai-LA(15 : ATGATACTTGGTTTTGTTCTGTTTGGTTTATGTGGGAGGGGTTTATGTACTCTTTATTTTTGTTTCTATGCATAATCCAAATCCTTCGCCTATGCTGGGTGGGAGTTATGGGTTGGTATTGTTTGTTTATTTTTTTTTGGCTATAAATTTTGTTGGCTATTTTACAGGCTATGAGGGTTC : 11160 * * 11180 * 11200 * 11220 * 11240 * 11260 * 11280 11300trnY * 11320 * 11340 Htai-LA(15 : TCTGGTGGAGTGTAGTCATTATCTCTGTAGGTATTTTGAGGGGATAACGTACTTGTTTTTTTGCACGATGTTGATGCTGGGTTTTGTGATAGTAAGAATAGTAGGGGGGGAGAAGTGTTCCTTTTATCGA TAG A ACTAACTTAGTATAAGGAGTACATCGGTTTGTAGGTCCGAGGGTGA : 11340 * * 11360trnL1 * 11380 * 11400 * 11420 trnS2 * 11440 * 11460 11480 * 11500trnL2 * 11520 Htai-LA(15 : TGATTGTTCAGTTGGGG TTAGAGGTGCCAGAGTTTTATGGGGCTGGTTTAGGTCCAGTTAACGATATAAATTATCCCTTTGCG GGCAAGGGTTTGTCCGTTGATGATTTGAAGTCATTAGTTTTTCTTTGTTAGGAATGCTCGCT TAGCTTGG ACATGGATGCCAGAGGCGTTAATGGGT : 11520 nad5 * * 11540 * 11560 * 11580trnR * 11600 * 11620 * 11640 11660 * 11680 * 11700 Htai-LA(15 : TGGTTTTAAGCATCAAATACGGGTGTTATTCCCTCCGTGTA TTGGTCCCGTG TTTGATAACCATTGTGTGGGGTTGCGTTTCGGCCGCAATGAAGGGTTAGATGAACTCTATCAAAA G GTG TTAAGGGCTGTTGTGGTGCTTTTTGTTGCGGTAATGCTTTTCTTGTTATCTATTCTTGG : 11700 * * 11720 * 11740 * 11760 * 11780 * 11800 * 11820 11840 * 11860 * 11880 Htai-LA(15 : TTATTGGGGGTGAGGGTGGTCTTTTTCGTGGTTAGGTTTTGTTTTTCGTGACTATGATTTTGTTTTTGTGGTAAGGGATGTGAGTTTGGTTTGTGAAGTAATGCTATTGTTCTGTGGTTCAGTGGCATTATTTTACTGTTTTCATTATTTTGGACAAGGCGCGGATGCTTTTTTTCTTTA : 11880 * * 11900 * 11920 * 11940 * 11960 * 11980 * 12000 12020 * 12040 * 12060 Htai-LA(15 : TCCATTGATGGCCTGGTTCTTAGGGGTCATGTCTATTCTGGTAATGAGAGGTAGGGTGCTCTTGTCTCTCTTGATGTGGGAATACTTAGGCTTGGTGAGGTTTTTTTTAATCTTATTTTATTCTAACATGGCAAGTCTTCGGGCTTCGTTAATAACTCTTTTTGTCTCACGTTTTGGGGA : 12060 * * 12080 * 12100 * 12120 * 12140 * 12160 * 12180 12200 * 12220 * 12240 Htai-LA(15 : TGCCGCCTTATTTATGGTGGTTATGTGGGTTAGATGGTGGTTTGGGGCTTCGTCGATTTTATTGGGATTGCTGcTATTTTTTATTGTTTTAACTAAGAGGGCTTGTTACCCGTTTATTTCGTGGTTACTGGAAGCTATGCGAGCTCCTACGCCAGTAAGTTCTCTTGTGCATTCTTCTAC : 12240 * * 12260 * 12280 * 12300 * 12320 * 12340 * 12360 12380 * 12400 * 12420 Htai-LA(15 : TTTGGTGGCCGCGGGAGTTTGATATCTTTTTTGTTATGGGGATATGTTTAGGTACGGAATGTTAAGGGTATTGTTTATGTTGAGGCTAGTTACTGTTTTAGTAAGGGGGTTTTGTGCTTTGGTATTTAAAGATTTGAAGAAGTTGGTGGCCCTTTCTACTTGTAAAAAAGTTTCTTGATG : 12420 * * 12440 * 12460 * 12480 * 12500 * 12520 * 12540 12560 * 12580 * 12600 Htai-LA(15 : TATACTGTTTTATGTTTGTGGAAGTCTGGATTTAGCTTTATTACAGTTGATAACACATGGGGTCTGTAAGTGTTACCTCTTTATGTCTATTGGGGACCTGATGGGCTTATCGGGGGGGAGGCAGAGTTCTGTGGGGGTTTATTTGTCCCGTTATGTGGGTCGGTTTTCAGTGTATTTACA : 12600 * * 12620 * 12640 * 12660 * 12680 * 12700 * 12720 12740 * 12760 * 12780 Htai-LA(15 : AAGCATTTTGGTGATATCCTTGAGAGGCCTCCCCTTcTTAGGGGTGTTTTTTAGGAAGCATATGTTGGTTGGCTCGGTTTGTTATGTTTATGGGTGAGGGTATTTTGGCTTAATGTTTTTGGGATTTTTTCTTTCTTATGCTTACTGTGTGCGGTTAGTATTGTTGCTTGTTAAGGGCCT : 12780 * * 12800 * 12820 * 12840 * 12860 * 12880 * 12900 12920 * 12940 * 12960 Htai-LA(15 : GGGAGGATTAAGAAGTGGTTATTCGAGTTCCTTTATTATTATTTCTGGTCTGAGCGTAGCGGGGACCTTGTTAAAATCGTGTGGAATGGGTGTGTTAGAAGAGAGTGCGGGATTACTTGTTAGTTATAGCTTTTTTGTAGGAATGATTCAGGTCTTGGGTTGTTTGGGAGGCTTCCTTTT : 12960 * * 12980 * 13000 * 13020 * 13040 * 13060 * 13080 13100 * 13120 * 13140 Htai-LA(15 : TTTTGTCGCTGGTGGGGAGGGGGTCCTTTTTGGGCCTTCTACTTTGAGTTATTCTGATAGGGTAGTTAGGGGGGTGTATGATTTTTATCTTCGTCTCAGTGCTCCTGCTGTTGTGTCTTTTTATCGTTGGGAGTATTATGTTTTTTCTTTGTGGCCACGGTTGACATCTCGTTTTCTCCT : 13140 Long Int seq * 13160 * 13180 * 13200 * 13220 Htai-LA(15 : TTTTCGGCTTTCTGCCTTTTCTTTTAAATCCTTGGTTTTGTTTTTGTTGTGTTTTTTATTCTTATACTTTCTAGTCGGTGTT * 13240trnE TAA ATTACGTTTGA * * 13260 13280 * 13300 * 13320 GTTGATTGGGTGTATATGCATTCTAAATTTTCGTTTTAGAGGAGGCCTTTTATTGGGGAGGGGCTAATCAATAATTTTAAATTT : 13320 (underlined) (259) * * 13340 * 13360 * 13380 * 13400 * 13420 * 13440 13460 * 13480 * 13500 Htai-LA(15 : TTAGTTTGATTTTTTTTAATTCTGTCTGAAAGATGCTTGTTTTTTTTATGGGGTGGTTGCCCTAGTTTATCTCTCTGTTGTGAAAACTGTAAGGGGGGGGTATTTTATGTTTTTAGACGTTGTGTTTTATTAGGGAGTTTTTGTGTGTAGAGGGTATAAATTTTATTTTGAAAAATTTAT : 13500 TRU1 (182) * 13520 * 13540 * 13560 Htai-LA(15 : TGTGGTTAGTTGTTTGATTGGAAGAGGTAAATAGAAGAAAGGAAATAGGGAGGGGGGGGACCCCCCCC * 13580 * 13600 * 13620 * 13640 * 13660 * 13680 TAATATCTATATTATTATACCTTTAGTTCTAAAAAAAGAAAAGGGAATAAAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGAAAGGGTGATTGTCTCGATG : 13680 TRU2 (182) * * 13700 * 13720 * 13740 * 13760 * 13780 * 13800 13820 * 13840 * 13860 Htai-LA(15 : CACTGGGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAAAAAGGAATAGGGAGGGGGGGGACCCCCCCCTAATATCTATTTTTTTATACCTTTAGTTCCAAAAGAAAAAGAGGGATAAGAATATTGCAAAGGGTAAGAAAAGGTTTGTTCCCGGGCACCGAAAGGGAGATTGTCTCGAT : 13860 * 13880 * 13900 * 13920 * Htai-LA(15 : ACACTGGGGTTTGTTTTTGATTGGCAGAGATTAATAGAAAAAAGGAATTGGGAGGGGGGGGGACCCCCCCC * 14060 * 14080 * 14100 * Long Int seq (underlined) * 13940 * 13960 * 13980 14000 * 14020 * 14040 CTAATATCTATATTATCATACCATTAGTTCTAAGAGAAAGAAGGAATAGAAATTGGCTAGGATAGATAGGTTTGATGCTTGTGCGCAGAGGGGAATGTCTCAACCACTG : 14040 14120 * 14140 * 14160 * 14180 * 14200 * 14220 Htai-LA(15 : GGTTATTGTTGTCGCAGATATAGAGAAGGATTGGAGGGGGCACCCCCGAATCATATTACACCTTGGTCAATAAAAAGGAATAAAAATTGCAGGAAGAATCGGACCTACGCTAATGTGGATTCCGACCTGTTATGTTCTTAAGCCATGAGAGGACCTTGTGGGTGACCCCTTTTTTCTTTT : 14220 * 14240 * 14260 * 14280 * 14300 * 14320 * 14340 * 14360 * 14380 * 14400 Htai-LA(15 : TAGCGGTGTTCTAAGAGATGAGGGATGACACTCGCAGGATGGTATGTTTGAGCCTCTCAGTGGAAGGGGGATTCCCCCACCACCCTGTTTAGTTTTTGATTGGAGAGACTATTTAGAAGAAAGGCAGTTGTGAGGGGGGGACCCCCCCCCTTTTTTTTTTTTTTTTCTTGCCCTTATTTT : 14400 * 14420 Htai-LA(15 : TAAAAGGAAAGAGGGGGGG Htai-LA(15 : * 14600 TRU3 (183) * 14440 * 14460 * 14480 * 14500 * 14520 * 14540 * 14560 * 14580 TAGAATATCTGGCAAGAGATTGGATAGGTTTGAAGCCTCTGCCGCGGAAAGGGGGATTTCCCGATGCACTGTGGTTAGTTGTTTGATTGGGAGAGGTTTATTGGAGAAAACGATTTTGGGAGGGGGGGGGACCCCCCCCCTTATATCTTTTTTTTCATACC : 14580 TRU4 (183) * 14620 * 14640 * 14660 * 14680 * 14700 * 14720 * 14740 * 14760 CCTATTTTTAAAAGAAGAAGAGGGGATAGAATATTGGCAAAGGATTGGATAGGTTTGAAGCCTGTGCAGCGGAAGGGTGATTTTCTCGATGCACTGTGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAGAAAGGAAATTGGGAGGGGGGGGACCCCCCCCTAATATTTATATTATCAT : 14760 TRU5 (183) * 14780 * 14800 * 14820 * 14840 * 14860 * 14880 * 14900 * 14920 * 14940 ACCATTAGTTTTAAAAGAAGAAGAGGGAATAGAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGGAAGGGTGATTTTCTCGATGCCCTGTGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAGAAAGGAAATAGGGAGGGGGGGGACCCCCCCCTAATATCTATATTAT : 14940 * 14960 * 14980 * 15000 * 15020trnG * 15040 * 15060 * 15080 * 15100 * 15120 Htai-LA(15 : CATACCCTTAGTTCTAAAAGAAGAAGAGGGAATAGAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGGAGGTAGTATAGGTTTGAGTACGCTGTCTTTCCAAGTCAGATGTCCCGTTGATCGGGTCTGTGCACCTTACTCTTAGTTTACTCTATTTGAGGCGGGTATGAA : 15120 * Htai-LA(15 : TGTGGGGGATG : 15131 Htai-LA(15 : nh 3 Trình ribosome, ph n m VN) (7.268 bp) bày di n giải gen v ng giao gen đ n ị h h a loài Haplorchis taichui Việt Nam (chủng Htai-QT3- 18S(1992) * 20 * 40 * 60 * 80 * 100 * 120 Htai-QT3- : AACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAGAGATTAAGCCATGCATGTCTAAGTACAAACCTTCAAACGGTGAAACCGCGAATGGCTCATTAAATCAGCTATGGTTCCTT : * 140 * 160 * 180 * 200 * 220 * 240 Htai-QT3- : AGATCGTACACTACTACATGGATAACTGTAGTAATTCTAGAGCTAATACATGCCACTATGCCCTGACCCGCAAGGGAACGGGTGGATTTATTAGAACAGAACCAACCGGTGGTGGCTTCG : * 260 * 280 * 300 * 320 * 340 * 360 Htai-QT3- : GCTGCTGCTGTTGCATTCTGTGATGACTCTGGATAACTTTACTGATCGCAGTCGGCCTTGTGTCGGCGACGGATCTTTCAAATGTCTGCCCTATCAATTTTCGATGGTAGGTGACCTGCC : * 380 * 400 * 420 * 440 * 460 * 480 Htai-QT3- : TACCATGGTGATAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACTTCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGGCACGGGG : * 500 * 520 * 540 * 560 * 580 * 600 Htai-QT3- : AGGTAGTGACGAAAAATACGAATACGGGACTCGTTAGAGGCTCCGTAATTCGAATGAGTACACTTTAAATCCTTTAACGAGGATCAACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT : * 620 * 640 * 660 * 680 * 700 * 720 Htai-QT3- : AACTCCAGCTCCAGAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATCTGGGTCGCATGGCTACATGCCGTTGCTTGTATTCCTGGCCTGGTTAACACCGGGACGGGT : * 740 * 760 * 780 * 800 * 820 * 840 Htai-QT3- : TTACGGGTCGGTGTCGTGGTTGTGCAGCCTTTCTGCCGTGTCTGTTTCGACAGGTGTTGGCATGATTGGTGGGTTCGTCCTACTGGTCTGTTGACATGCTCCCAGGTGCCCTTTACTGGG : * 860 * 880 * 900 * 920 * 940 * 960 Htai-QT3- : TGTCGGGGGCGGACGGCACGTTTACTTTGAACAAATTTGAGTGCTCAAAGCAGGCCAATGTGCCTGAAAAGTCTTGCATGGAATAATGGAATAGGACTTCGGTTCTATTTTGTTGGTTTT : * 980 * 1000 * 1020 * 1040 * 1060 * 1080 Htai-QT3- : CGGATCCGAAGTAATGGTTAAAAGGGACAGACGGGGGCATTTGTATGGCGGTGTTAGAGGTGAAATTCTTGGATCGCCGCCAGACAAACTACAGCGAAAGCATTTGCCAAGGATGTTTTC : * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 Htai-QT3- : ATTGATCTGGAGCGAAAGTCAGAGGTTCGAAGACGATCAGATACCGTCCTAGTTCTGACCATAAACGATGCCAACTGACGATCCGTGGTGGCGCGATTATTGGCCCCGCGGGCAGTCCCC : * 1220 * 1240 * 1260 * 1280 * 1300 * 1320 Htai-QT3- : GGGAAACCTTTAAGTCTTTGGGCTCCGGGGGAAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCGACTCAACACGGG : * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 Htai-QT3- : AAAACTCACCCGGCCCGGACACTGTGAGGATTGACAGATTGATAGCTCTTTCTTGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT : * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 Htai-QT3- : AACGAACGAGACTTTGGCCTGCTAAATAGTACGCCTGTCCTCTGCGCTTGTGCAGGTGGCGGTGATCGTTGCCTCTCTGGGGTGATGGTGCCGTTTGCCGGCGAGTGCGGCGCAGGTGTC : * 1580 * 1600 * 1620 * 1640 * 1660 * 1680 Htai-QT3- : TACTTCTTAGAGGGACAAGCGGCGTGCCAGTCGCACGAAATAGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACGTGCGCTACAATGACGGTTTCAACGAGTTTGGG : * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 Htai-QT3- : ATCCTAGCCCGAAAGGGTTGGGTAAACTGAATCATAACCGTCGTGACTGGGATCGGGGCTTGCAATTATTCCCCGTGAACGAGGAATTCCTGGTAAGTGCAAGTCATAAGCTTGCGCTGA : * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 Htai-QT3- : TTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGCAAGGTCCTCGGATTGGTGCCATTGTAGTGGCTTCGGCTGCTCGACCGGTGCTGAAAAGACGA : 120 240 360 480 600 720 840 960 1080 1200 1320 1440 1560 1680 1800 1920 ITS1(797) * 1940 * 1960 * 1980 * 2000 * 2020 * 2040 Htai-QT3- : CCAAACTTGATCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCT GCGGAAGGATCATT ACAGTACAC CTAACCGTTATCATCCCGGTTTTCCCGGGTGGTAAGTTC : 2040 * 2060 * 2080 * 2100 * 2120 * 2140 * 2160 Htai-QT3- : GGGCTGTCGTGCTCGTACAATCTGCAGCTTGTCTGCCTAAGGCGGAGCGATGAGTTCCGTCATATGTGCTTGTATTTCAGAGATGGAGCGACGCCAGCTCTGTCAAATCCAGTATGCATT : 2160 * 2180 * 2200 * 2220 * 2240 * 2260 * 2280 Htai-QT3- : GCATATTGTTGCCTCCATGGCTGACCTCTAACTCGGTAAAGCGACGCCAGCTCTATCGAGTCTCTTGCCCGTTGTTTCTTCGAAGTTGGGCTTTAATTATGCAGCCTTGTCTGCCTAAGG : 2280 * 2300 * 2320 * 2340 * 2360 * 2380 * 2400 Htai-QT3- : CGAAGCGATCAGTTTCGTCATGAGTATTGCACTGGTAGATGGAGCGACGCCAGCTCTATCAAATCTTGTATTCATTGGTTTTAATTTGGCTTTTGGCCTTTGATTACGCAGCTTTGTCTG : 2400 * 2420 * 2440 * 2460 * 2480 * 2500 * 2520 Htai-QT3- : TCGCTGGGCTATACCTGGTACACACGTGTACCTACGTACAGTCCTTGTTTTGGCGGGGTGTCTACCTGTCTGATGCTCTTGGTGTGCTCGCTCACAGTAGCCAGTCCACACCGGGAGTGA : 2520 * 2540 * 2560 * 2580 * 2600 * 2620 * 2640 Htai-QT3- : CGGGTTGTGCTGCTAATATTGGCAGTGCTAGGCTTAAAGAGTGGGCTCGAATATTGTGAAGGGCTACGGCCCACCCACCGCCCCGTATCACGTTGTTTTAAACATATTTTACACTGTTTA : 2640 * 2660 * 2680 * 2700 * 2720 * 2740 * 2760 Htai-QT3- : AGTGAGCAGGCATGCTTGTCTTGCTTGTTCTCACTGCCCCGACATGCACCTGGTGCTTGACACTGGACTGCATGTGCAGTCGCCCGGCGGTGCCTTATCCCGGGTACGACTTGCAATCCC : 2760 5.8S(160) * 2780 Htai-QT3- : GGTCATCTGGTTTACCATTTGATCGATGA * 2800 * 2820 * 2840 * 2860 * 2880 GTACAACTCTGAGCGGTGGATCACTCGGCTCGTGTGTCGATGAAgAgCGCAGCCAACTGTGTGAATTAATGTGAACTGCCCAAAGCTTTGA : 2880 * 2900 * 2920 * 2940 Htai-QT3- : ACATCGACATCTTGAACGCACATTGCGGCCATGGGTTTTCCTGTGGCCACGCCTGTCCGAGGGTCGGCT ITS2(444) (3 TRU) * 2960 * 2980 * 3000 TATAAACTATCACGACGCCCAAATAGTCGTGGCTTGGGTCTTGCCAGCTGG : 3000 TRU1(85) * 3020 * 3040 * 3060 * 3080 * 3100 * 3120 Htai-QT3- : CGTGATTTCCTTGTGCTTTTGCATAGGGTGCCAGATCTATGGCTTTTCCCTAATGTGCCGGACGCAACCA TGTCTTGGCTGACGGCCTGGATGAGGAAGTGGCGGCGGAGTCGTGGCTCA : 3120 TRU2(83) TRU3(85) * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 Htai-QT3- : ATGAAAATTGTCCGCGCGCTCCAAAGCTTAACCTC TGTCTGGGCTGACGGCTTGGATGAGGAAGTGGCGGCGGAGTCGTGGCTCAATGAAAATTGTGCGCGCTCCAAAGCTTAACCTC TG : 3240 * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 Htai-QT3- : TCTGGGCTGACGGTTTGGATGAGGAAGCGGCGGCGGAGTCGTGGCTCAATGAAAATTGTCCACGCGCTCCAAAGTTTAACCTC TGTCTGAG CTGACGGCTAAGATGTGGCAATGCATCCG : 3360 28S(3875) * 3380 * 3400 * 3420 * 3440 * 3460 * 3480 Htai-QT3- : ATGCAATTCTTTGTGCACTTG AATGTGCCTTAT TCCTGACCTCGGATCAGACGTGAATACCCGCTGAACTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGT : 3480 * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 Htai-QT3- : AACGGCGAGTGAACAGGGAAAAGCCCAGCACCGAAGCCTGTAGCCGTTTGGTTACTAGGCAATGTGGTGTTCAGGTCGTCCCGCGAAGGTACTGCTCCATCCCAAGTCCAGCAATGAGTA : 3600 * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 Htai-QT3- : CGGTAATATTTACATGGCCCAAAGAGGGTGAAAGGCCCGTTGGGGTGGAGGCGCAGTAATGCCAGTGCTTTCCTGGGTAGACCTTGGAGTCGGGTTGTTTGAGAATGCAGCCCAAAGTGG : 3720 * 3740 * 3760 * 3780 * 3800 * 3820 * 3840 Htai-QT3- : GTGGTAAACTCCATCCAAAGGCTAAATACTAACACGAGTCCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTACGTGAAACCGCTCAGGAG : 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 Htai-QT3- : GTAAACGGGTGGAGTTGAACTGTGATCTCTGTGAATTTAGCTGGTGAGCATGGTTTGAGCTTGGTCAAATTGGTTGTTACTCGGGGTCTGCTTAGCTGCAGGTCTTTGCCTATGGTGAAG : 3960 * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 Htai-QT3- : ATGCGCGAGGCACTTGTCAGGTGTTGCGCGCCCCATGTAATTCAGGCCAGCTTGCCAGTGCACTTTCGCAGAGTGTTCACCACGACCGGCATCGCTGTCCGGCTGATGTGGTTAAACCGG : 4080 * 4100 * 4120 * 4140 * 4160 * 4180 * 4200 Htai-QT3- : TCTCGCATTGTCCTTGTGGCTTTGCTTGGTCGGGATGGCAGGTAGCCCTTGGACTTGCTCGCAGCTTGCTGTGGGTTCAGGTCCTTGGGTGTAATCAGCTGACTACATCGGTTCTGTGCA : 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 Htai-QT3- : GTATGTCGGAGACGGCGGCTTGTGGTGTGTGTGCGTGCGTACCAGTTGACTGGCGTGTCCGGGTTTGGTTATTATGTTGCCTGTGTTTACAGGCCTGGTGATGGCCCGGGTTCGTTCGGT : 4320 * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 Htai-QT3- : TGACGGTTGCGTGCGTGTCACTTTCCAGGGCCAATAGTCTGTGGTGTTGCGGTAGACTTTCCACCTGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTACGCGAGTCATTGGGC : 4440 * 4460 * 4480 * 4500 * 4520 * 4540 * 4560 Htai-QT3- : GTAACGAAACCCAAAGGCGAAGTGAAAGTAAAGGTCCGGCTTGTCTGGACTGAGGTGAGATCCTGTCGTTTCTCACACGTGGTATCACCAAGTATCGAGCGACAGGCGCATCACCGGCCC : 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 Htai-QT3- : GTCCCATGGTGTGGTGACATTTTTTGGTGTTCGTCACCGGGGCGGAGCATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAACTCTGG : 4680 10 * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 Htai-QT3- : TGGAGGACCGCAGCGATTCTGACGTGCAAATCGATCGTCAGACGTGAGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGTG : 4800 * 4820 * 4840 * 4860 * 4880 * 4900 * 4920 Htai-QT3- : TTCAGAAATATAAACAGTTTTATCCGGTAAAGCGAATGATTAGAGGCCTTGGGGGCGAAACGTCCTCAACCTATTCTCAAACTTTAAATGGGTGAGAAGCCTGGCTCGCCCAAACGGAGT : 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 Htai-QT3- : CAGGCGTTGAATGTGAATGCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGATAAGGTGCCTAACGCGACGCTCATCAGATACCACAAAAGGTG : 5040 * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 Htai-QT3- : TTGGTTGGTCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAATGTGTAACAACTCACCTGCCGAATCAACCAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGACCT : 5160 * 5180 * 5200 * 5220 * 5240 * 5260 * 5280 Htai-QT3- : ATACCGAACCGTTACTGCAAGCTGAGTCGATAATGTGTGGTGTGAGGACAAGCCGCACGACTCAGGCACGGAATTGTGGTGACGAGTAGGAGGGTCGCCGTGGTGAGCGGGAAGCTTCGG : 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 Htai-QT3- : GCGTAAGCCTGAGTGGAGCCGCCACGGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGGCCGAGGTGGAGAAGGGTTCCATGGGAACAGCAGCTGGCCATGGG : 5400 * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 Htai-QT3- : TCAGGCGGTCCTAAGTGATCCGTTAACTCGGAACAGAGACGGGGTGCCTGACATTGTCAGGTTTGCTGCTGATGTACTGTTGCAAGGCAGCCCCCTGTAGCGAAAGGGAATCGGGTTAAT : 5520 * 5540 * 5560 * 5580 * 5600 * 5620 * 5640 Htai-QT3- : ATTCCCGACCCTGCCCACGGAGATTGGTGCATTCTGGTCTTGTACTGGTTTGTACCTAGCGCGGTAACGCAACCGACCTCGGAGACGTCGGCAAGAGTTCTGGGAAGAGTTCTCTTTTCT : 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 Htai-QT3- : TTATTAGGAGTGCATCATCCCCTGGAATCGGGTTGTCCGGAGATAGGGGACTAGCCTCCGTAAAGCACCACATTTCATGTGGTGTCCGGAACGCTCTTGTCGGCCCTTGAAAATCCGAGC : 5760 * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 Htai-QT3- : AAGGCAATATAATTTTCGTGGCAGGCCGTACCCATATCCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCAATTGAACAATGTAGGTAAGGGAAGTCGGCAAACTGGATCCGTAACTTC : 5880 * 5900 * 5920 * 5940 * 5960 * 5980 * 6000 Htai-QT3- : GGGAAAAGGATTGGCTCTGAGGGCCGAGTCAAATGGGCTGGAGTCAGAAACGTTTCGGTCCAGTTAGAAGCTGGTTTTTCTGTTGCTGCGTTTGCCTTCGGGTGAGTGCGGTTGATGGAA : 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 Htai-QT3- : TTACTGGAGTCTGACCAGGCCGATCGTGGGCGATTTCAGCTGTAGTGCTGCTTGGCTGGCAGCGTGCAGGCAACTGTGCGTGTCGACGGCCTTGCTTCCTTCGTTTGGCGCTCAACGGCT : 6120 * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 Htai-QT3- : AGCTCAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGTCCGCTGACCGGTTTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG : 6240 * 6260 * 6280 * 6300 * 6320 * 6340 * 6360 Htai-QT3- : AAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCTACTGTCC : 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 Htai-QT3- : CTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAGGAGACATGACGGGTGTAGA : 6480 * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 Htai-QT3- : ATAAGTGGGAGATGGGCTTCGGTTCATCGCCAGTGAAATACCACTACCGTCATTGTTTCTTTGCTTATTCAGTAAAACGGGAATGATATTTTGGTTCTAAGCACATGCTGGCATGCGTGG : 6600 * 6620 * 6640 * 6660 * 6680 * 6700 * 6720 Htai-QT3- : TTCTGCTGCGTGTGTCGGTATCTTGGTCATGTCAGGTGAGTCGTGACTTCGGTTGCGGCGTTTGAGCCTGGATGTGATGTGCGACCCGTTCTGAAGACAATGTCAGGCGGGGAGTTTGAC : 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 Htai-QT3- : TGGGGCGGTACATCTGTCAAATGGTAACGCAGGTGTCCCAAGGTGAGCTCAGCCAGGACGGAAACCTGGCGTAGAGTAAAAGGGCAAAAGCTTACTTGATTTTGATTTTCAGTACGAATA : 6840 * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 Htai-QT3- : CAGACCGTGAAAGCGCGGCCTATCGATCCTTTTGATTTTGATATTCAGAGTTTGAAGCAAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCG : 6960 * 6980 * 7000 * 7020 * 7040 * 7060 * 7080 Htai-QT3- : ACGTCGCTTTTTGATCCTTCGATGTCGGCTCTCCCTAGCGTTGTGACGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGAC : 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 Htai-QT3- : AGGTTAGTTTTACCCTACTAATGAGTACAATGAATCAGTGAAGTACAAACTGGCCGTTGCTATGGTAATCCTGTTTAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTATATGTGCCT : 7200 * 7220 * 7240 * 7260 Htai-QT3- : GGCCGATAGGCCAATGGTGCGAAGCTACCATCTGAGGGATTAGGACTGAACGCCTCTAAGTCTGAATC : 7268 // // IGS nh Trình bày di n giải gen v ng giao gen đ n ị h ribosome, ph n m h a loài Haplorchis pumilio Việt Nam (chủng Hpum-HPU8-VN) (7.416 bp) 18S(1992) * 20 * 40 * 60 * 80 * 100 * 120 HPU8-rTUc : AACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAGAGATTAAGCCATGCATGTCTAAGTACAAACCTTCAAACGGTGAAACCGCGAATGGCTCATTAAATCAGCTATGGTTCCTT : 120 * 140 * 160 * 180 * 200 * 220 * 240 HPU8-rTUc : GGATCGTACACTACTACATGGATAACTGTAGTAATTCTAGAGCTAATACATGCCACTATGCCCTGACCCGCAAGGGAACGGGTGGATTTATTAGAACAGAACCAACCGGTGGCGGCTCCG : 240 * 260 * 280 * 300 * 320 * 340 * 360 HPU8-rTUc : GCTGCTGCTGTTGCATTCTGTGATGACTCTGGATAACTTTACTGATCGCAGTCGGCCTTGAGTCGGCGACGGATCTTTCAAATGTCTGCCCTATCAATTTTCGATGGTAGGTGACCTGCC : 360 * 380 * 400 * 420 * 440 * 460 * 480 HPU8-rTUc : TACCATGGTGATAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACTTCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGGCACGGGG : 480 * 500 * 520 * 540 * 560 * 580 * 600 HPU8-rTUc : AGGTAGTGACGAAAAATACGGATACGGGACTCGTTAGAGGCTCCGTAATTCGAATGAGTACACTTTAAATCCTTTAACGAGGATCAACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT : 600 * 620 * 640 * 660 * 680 * 700 * 720 HPU8-rTUc : AACTCCAGCTCCAGAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATCTGGGTCGCATGGCTACATGCTGTTGCTTGTATTCCTGGCCTGGTTTACACTGGGACGGGT : 720 * 740 * 760 * 780 * 800 * 820 * 840 HPU8-rTUc : TTACGGGTCGGCGTCGTGGTTGTGCAGCCTTTCTGCCGTGTCTGTTTCGACAGGTGTTGGCATGATTGGTGGGTTCGTCCTGCCGGTCTGTTGACATGCTCCCAGGTGCCCTTTACTGGG : 840 * 860 * 880 * 900 * 920 * 940 * 960 HPU8-rTUc : TGTCGGGGGCGGACGGCACGTTTACTTTGAACAAATTTGAGTGCTCAAAGCAGGCCCATGTGCCTGAAAAGTCTTGCATGGAATAATGGAATAGGACTTCGGTTCTATTTTGTTGGTTTT : 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 HPU8-rTUc : CGGATCCGAAGTAATGGTTAAAAGGGACAGACGGGGGCATTTGTATGGCGGTGTTAGAGGTGAAATTCTTGGATCGCCGCCAGACAAACTACAGCGAAAGCATTTGCCAAGGATGTTTTC : 1080 * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 HPU8-rTUc : ATTGATCTGGAGCGAAAGTCAGAGGTTCGAAGACGATCAGATACCGTCCTAGTTCTGACCATAAACGATGCCAACTGACGATCCGTGGTGGCGCGATTATTGGCCCCGCGGGCAGTCCCC : 1200 * 1220 * 1240 * 1260 * 1280 * 1300 * 1320 HPU8-rTUc : GGGAAACCTTTAAGTCTTTGGGCTCCGGGGGAAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCGACTCAACACGGG : 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 HPU8-rTUc : AAAACTCACCCGGCCCGGACACTGTGAGGATTGACAGATTGATAGCTCTTTCTTGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT : 1440 * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 HPU8-rTUc : AACGAACGAGACTTTGGCCTGCTAAATAGTACGCCTGTCCTCTGTACTCGTGCAGGTAGCGGTGGTCGTTGCCTCTCTGGGGTGATGCTGCCGTTTGCCGGCGGGTGCGGCGCAGGTGTC : 1560 * 1580 * 1600 * 1620 * 1640 * 1660 * 1680 HPU8-rTUc : TACTTCTTAGAGGGACAAGCGGCGTGCCAGTCGCACGAAATAGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACGTGCGCTACAATGACGGTTTCAACGAGTTTGGG : 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 HPU8-rTUc : ATCCTAGCCCGAAAGGGTTGGGTAAACTGAATCATAACCGTCGTGACTGGGATCGGGGCTTGCAATTATTCCCCGTGAACGAGGAATTCCTGGTAAGTGCAAGTCATAAGCTTGCGCTGA : 1800 * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 HPU8-rTUc : TTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGCAAGGTCCTCGGATTGGTGCCATTGTAGTTGCTTCGGCAGCTCGACCGGTGCTGAAAAGACGA : 1920 ITS1(1106) * 1940 * 1960 * 1980 * 2000 * 2020 * 2040 HPU8-rTUc : CCAAACTTGATCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATT ACAGAATGCCTAA CCTATATCATCCTGGTTTATCCAGGTGGTGTGTGA : 2040 TRU1 (136) * 2060 * 2080 * 2100 * 2120 * 2140 * 2160 HPU8-rTUc : GTTCTCATGCTCATACGA AAACAAAAATGTGAACCTTGGAAGGATCATTACAGAATTCTAACCTGGACCCTTCGGGCTTGCTATCATATACCAGTGCTACTGGTCTGCAGCTTGGCTGCC : 2160 TRU2(136) * 2180 * 2200 * 2220 * 2240 * 2260 * 2280 HPU8-rTUc : TACGGCAGAGCGCTTTTCAGTTCTGTCATTGTTC AAACAAAAATGTGAATCCTGGAAGGATCATTACAGAAGTCTAACCTGGACCCTTCGGGCTTGCTATcaTATACCAGTGCTACTGGT : 2280 TRU3(136) * 2300 * 2320 * 2340 * 2360 * 2380 * 2400 HPU8-rTUc : CTGCAGCTTGGCTGCCTACGGCAGAGCGCTTTTCAGTTCTGTCATTGTTC AAACAAAGATGTGAATCCGCGAAGGATCATTACAGAAGCCTAACCTGGACGCTTCGGGCTTGCTATCATA : 2400 TRU4(116) * 2420 * 2440 * 2460 * 2480 * 2500 * 2520 HPU8-rTUc : TACCAGTGCTACTGGTCTGCAGCTTGGCTGCCTATGGCAGAGCGCTTTTCAGTTCTGTCATTGTTC ATATACGTTGAATTTATATATATATTTGTGAATGGACCCTTCGGGCTTGCTATC : 2520 * 2540 * 2560 * 2580 TRU5(116) * 2600 * 2620 * HPU8-rTUc : ATATACCAGTGCTACTGGTCTGCAGCTTGGCTGCCTACGGCAGAGCGCTTTTCAGTTCTGTCATTGTTCATATACGTTGAATTTATATATATATTTgTGAATGGACCCTTCGGGCTTGCT : 2640 11 2640 Int Seq (393) * 2660 * 2680 * 2700 * 2720 * 2740 * 2760 HPU8-rTUc : ATCATATACCAGTGCTACTGGTCTGCAGCTTGGCTGCCTACGGCAGAGCGCTTTTCAGTTCTGTC TTCGGGCTATACCTGGTATAAACCTCGTATATACCTACGTACAGTCCGTGTTTCG : 2760 * 2780 * 2800 * 2820 * 2840 * 2860 * 2880 HPU8-rTUc : GCGGGGTGTCTACCTGTCTGATACTCTTGGTGTGCTCGCTTATACAGCCAGTCCACACTGGGAGGACGGGTTGTGCTGCTGTTATGGGCAGTGCTAGGCTTAAAGAGTGGGCTCGAATAT : 2880 * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 HPU8-rTUc : TGTGAAGGGCTACGGCCCACCCACCGCCCCGTATCATACTGTTTTCAACCCATTTTACACTGTTTAAGTGAGCAGGTATGCTGTTTTGGCTGTCTGTTCTCACTGCCCCGACATGCACCT : 3000 5.8S(160) * 3020 * 3040 * 3060 * 3080 * 3100 3120 * HPU8-rTUc : GGTGCTTGACACTGGACTGCATGTGCAGTCGCCTGGCGGTGCCTTATCCCGGGTACGACTTGCAATCCCGGTCATCTGGGTTACCATTTGATCGATGA GTACAACTCTGAGCGGTGGATC : 3120 3240 * 3140 * 3160 * 3180 * 3200 * 3220 * HPU8-rTUc : ACTCGGCTCGTGTGTCGATGAAGAGCGCAGCCAACTGTGTGAATTAATGTGAACTGCCCAAAGCTTTGAACATCGACATCTTGAACGCACATTGCGGCCATGGGTTTTCCTGTGGCCACG : 3240 ITS2(280) * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 HPU8-rTUc : CCTGTCCGAGGGTCGGCT TATAAACTATCACGGCGCCCAAAAAGTCGTGGCTTGGGTCTTGCCAGCTGGCGTGATTTCCTTGTGCTATTGCATGGGGTGCCAGATCAATGGCTTCTCCCT : 3360 3380 * * 3400 * 3420 * 3440 * 3460 * 3480 HPU8-rTUc : AATGTGCCGAACGCAACCATGTCTGGGTTGAATGCCTGGATGAGGGGGTGGCGGCGGAGTCGTGGCTCAATTTATGTATTTTTTATTCATTGTGCGCGCTCCGCTGAAAACCTTCGTCTG : 3480 28S(3870) HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : HPU8-rTUc : * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 TGGCTGTGATGCTAGGATGTGGCAATGCATTCGATGCAAATAATTGTGCACATATGTGCCATATTT ACCTGACCTCGG ATCAGACGTGAATACCCGCTGAACTTAAGCATATCACTAAGC : 3600 * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 GGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACAGGGAAAAGCCCAGCACCGAAGCCTGTGGCCGTTTGGTTACTAGGCAATGTGGTGTTCAGGTCATTCCGTGAA : 3720 * 3740 * 3760 * 3780 * 3800 * 3820 * 3840 GGTACTGCTCCATCCCAAGTCCAGCAATGAGTACGGTAATTTTTACATGGCCCATAGAGGGTGAAAGGCCCGTTGGGGTGGAGGCGCAGCAATGCCAGTGCTTTCCTGGATAGACCTTGG : 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 AGTCGGGTTGTTTGAGAATGCAGCCCAAAGTGGGTGGTAAACTCCATCCAAGGCTAAATACTAACACGAGTCCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGA : 3960 * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 GAGAGTAAACAGTACGTGAAACCGCTCAGAGGTAAACGGGTGGAGTTGAACTGTGATCTCTGTGAATTTAGCTGGTGAACGTGGTTTGAACTTGGTCAAATTGGTTGTTATTTGGGGTCT : 4080 * 4100 * 4120 * 4140 * 4160 * 4180 * 4200 GCTTAGCTGCAGGTCTCCGCCTATGGTGGAGATGCGCGAGGCACTTATCAGGTGTTGCGCGCCTCAGGTGAATCGGACCAGTTCGCCAGTGCATTTTCACAGAGTGTTCACCACGACCGG : 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 CATCGCTGTCCGGCTGGTATGGTTAAACCGGTCTTGCATTGTCCTCGTGGCTTTGCTTGGTCGGGATGGCAGGTAGCTCTTGGGCTTGCTCGCAGTTCGCTGTGGGTTCAGGTCCTTGAG : 4320 * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 TGTAAACAGCTGACCGTATCGGTTCTGTGCAGTATGTCGGAGACGGCGGCTTGTGGTGTGTGCGTGCGTGCCAGTTGGCTGGCGAGTCCGGGTTTGGTTATTATGTTGCCTGTGTTGGCA : 4440 * 4460 * 4480 * 4500 * 4520 * 4540 * 4560 GGCCTAATGATAGCTCGGATTCGTTCGGCTGACGGTTGCGTGCGTGTCACTTTCCAGGGCCAATAGTCTGTGGTGTTGCGGTAGACTTTCCACCTGACCCGTCTTGAAACACGGACCAAG : 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 GAGAGTAACATGTACGCGAGTCATTGGGCGTAACGAAACCCAAAGGCGAAGTGAAAGTAAAGGTCTGGCTTGTCTAGACTGAGGTGAGATCCTGTCGTTTCTCACACGTGGTATCCCCAA : 4680 * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 GTATCGAACGGCAGGCGCATCACCGGCCCGTCCCATGGTGTGGTGACACTTCAGTGTTCGTCATCGGGGCGGAGCATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGC : 4800 * 4820 * 4840 * 4860 * 4880 * 4900 * 4920 GCAGGTTGAAGCCAGAGGAAACTCTGGTGGAGGACCGCAGCGATTCTGACGTGCAAATCGATCGTCAGACGTGAGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCT : 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 CCGAAGTTTCCCTCAGGATAGCTGGTGTTCAGAAATATAAACAGTTTTATCCGGTAAAGCGAATGATTAGAGGCCTTGGGGGCGAAACGTCCTCAACCTATTCTCAAACTTTAAATGGGT : 5040 * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 GAGAAGCTTGGCTCGCCCAAACGGAGTCAAGCGTTGAATGTGAATGCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGATAAGGTGCCTAACGC : 5160 * 5180 * 5200 * 5220 * 5240 * 5260 * 5280 GACGCTCATCAGATACCACAAAAGGTGTTGGTTGGTCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAATGTGTAACAACTCACCTGCCGAATCAACCAGCCCTGAA : 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 AATGGATGGCGCTGGAGCGTCGGACCTATACCGAACCGTTGCTGCAAGCTGAGTCGATAATGTGTGGTGTGAGGGCAAGCCGCACGACTCAGGCACGGAATCGTGGTGACGAGTAGGAGG : 5400 * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 GTCGCCGTGGTGAGCGGGAAGCTTCGGGCGTAAGCCTGAGTGGAGCCGCCACGGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGGCCGAGGTGGAGAAGGGT : 5520 * 5540 * 5560 * 5580 * 5600 * 5620 * 5640 TCCATGGGAACAGTAGCTGGCCATGGGTCAGGCGGTCCTAAGTGATCCGTTAACTCGGAACAGAGACGGGGTGCCTGACAATGTCAGGTTTGCTGCTGATGTACTGTTGCAAGGCAGCCC : 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 CCTGTAGCGAAAGGGAATCGGGTTAATATTCCCGACCCTGCCCACGGAGATTGGTGCATTCTGGTCTTGTGCTGGTTTGTACCTAGCGCGGTAACGCAACCGACCTCGGAGACGTCGGCA : 5760 * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 AGAGTTCTGGGAAGAGTTCTCTTTTCTTTATTAGGAGCGCATCATCCCCTGGAATCGGGTTGTCCGGAGATAGGGGACTAGCCTCCGTAAAGCACCACATTTCATGTGGTGTCCGGAACA : 5880 * 5900 * 5920 * 5940 * 5960 * 5980 * 6000 CTCTTGTCGGCCCTTGAAAATCCGAGCAAGGCAATATAATTTTCGTGGCAGGCCGTACCCATATCCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCAATTGAACAATGTAGGTAAGGG : 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 AAGTCGGCAAATTGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCCGAGTCAAATGGGCTGGAGTCAGAAACGTTTCGGTCAAGTTAGAAGCTGGTTTTTCTGTTACTGCGTTC : 6120 * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 GCCTTCAGGTGAGTGCGGTCGATGGGAATTCTGGAGTCTGACCAGGCCGATCGTGGGCGATTTCAGCTGTAGTGCTGCATTGCTGGCAGCGTGCAGGCAACTGTGCGTGTCGACGGCTTT : 6240 * 6260 * 6280 * 6300 * 6320 * 6340 * 6360 GCTTCCTTCGTTTGGCGCTCAACGGCTAACTCAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGTCCACTGATCGGTTTTGACGCAATGTGATTT : 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATG : 6480 * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 AATGGATTAACGAGATTCCTACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGAAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGAC : 6600 * 6620 * 6640 * 6660 * 6680 * 6700 * 6720 TTTGTGAGGAGACATGACGGGTGTAGAATAAGTGGGAGATGAGCTCCGGCTTGTCGCCAGTGAAATACCACTACCGTCATTGTTTCTTTGCTTATTCAGTAAAACGGGATACACTATTTT : 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 GATTCTAAGCACATGCTGGCATGCGTGGTTCTGCTGCGTGTGTCGGTATCTTGGTCATGTCAGGGTAGTCGTGACTTCGGTTGCGGCGTTTGAGCCTGGATGTGATGTGCGACCCGATCT : 6840 * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 GAAGACAATGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCCAAGGTGAGCTCAGCCAGGACGGAAACCTGGCGTAGAGTAAAAGGGCAAAAGCT : 6960 * 6980 * 7000 * 7020 * 7040 * 7060 * 7080 TACTTGATTTTGATTTTCAGTACGAATACAGACCGTGAAAGCGCGGCCTATCGATCCTTTTGATTTTGATATTCAGAGTTTGAAGCAAGAGGTGTCAGAAAAGTTACCACAGGGATAACT : 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 GGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTCCCTAGCGTTGTGACGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAAC : 7200 * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 GTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTAATGAGTACAATGAATCAGTGAAGTACAAACTGGCCGTTGCTATGGTAATCCTGTTTAGTACGAGAGGAACCG : 7320 * 7340 * 7360 * 7380 * 7400 * HPU8-rTUc : CAGGTTCAGACATTTGGTATATGTGCCTGGCCGATAGGCCAATGGTGCGAAGCTACCATCTGAGGGATTAGGACTGAACGCCTCTAAGTCTGAATC : 7416 12 // IGS // PL2.1 P ương p áp tá ết DNA tổng số từ mẫu ng ên ứu DN tổng số mẫu riêng biệt c tách chi t sinh phẩm DNeasy Blood and Tisue kit (Qiagen Inc M ) ho c GeneJET Genomic DN Purification Kit (Thermo Scientific Inc , M , US ) theo hư ng dẫn nhà sản u t DN tổng số c tách chi t từ SLRN Haplorchis spp nguyên sán trư ng thành thu nhận v i dung t ch hoảng –100 µL/mẫu n n n p m DNeasy Blood and Tissue kit (Qiagen Inc ỹ) : - M i mẫu sán ruột nhỏ ( con) c nghi n µL dung d ch Tissue Lysis buffer (ATL) o 56 C gi cho n hi mơ bổ sung µL proteinase-K ( c ph n giải hoàn toàn - Ti p tục bổ sung µL dung d ch - Thêm µL Ethanol ( sang cột c màng l c ly t m Llc mg ml) ủ o uủ 56 C/30 phút %), trộn u, sau chuyển tồn h n d ch vịng ph t ph t, lo i bỏ ph n d ch ph a dư i cột l c - Thêm µL dung d ch Washing buffer vòng ph t - Ti p tục thêm rửa DN , ly t m 3 µL dung d ch Washing buffer ( vòng ph t o –20 C cho i, ly t m l i ể làm hô màng hoảng 2–3 ph t, sau ph t Bỏ cột, thu d ch bên dư W2) vào cột l c ể ph t, bỏ d ch bên dư - Chuyển cột sang Eppendorf m i, thêm mẫu DN W ) vào cột l c, ly t m ph t, bỏ d ch ly t m bên dư i cột l c vòng ph t ph t (EL), ể nhiệt ộ phịng ( µL dung d ch Elution buffer ly t m i DN tổng số, ghi vòng ph t hiệu mẫu bảo quản n hi sử dụng - Kiểm tra hàm lư ng DN (OD, optical density) máy tổng số cách o h p phụ quang h c o quang phổNanoDrop®ND-1000 UV-Vis Spectrophotometer (Thermo Scientific Inc.) DN tổng số c pha thành nồng ộ ng µL, sử dụng 1–2 µL cho m i phản ứng PCR có dung tích 50 µL PL2.2 P ương p áp t ết ế m ung v Các mồi chung (g i mồi m u mtDNA ty thể chung cho sán 13 d t, universal- platyhelminth primers) c thi t ể thu toàn mtDN sán d t c SLRN c liệt ê công bố trư c y (Le et al., 2; 6; 9) ho c cải ti n bổ sung thêm ể thực PCR dài (long-PCR L-PCR) v i mục tiêu thu nhận ộ dài cao nh t c PL2 o n mtDN c ) Một số c p mồi c hiệu mtDN dụng b ho c dài h n (Bảng H taichui thu nhận sản phẩm PCR ng n ( ,5–3 huôn sản phẩm LPCR thể, 4–6 b) từ huôn DN c thi t tổng số ho c từ c thu nhận primer ể giải trình tự Chu i mồi c thi t ể c sử chư ng trình Primer3Plus (https://www.bioinformatics.nl/cgi-bin/primer3plus/) n n nm Sử dụng c p mồi: i) URNLF URNSR thu v ng gen rrnL-rrnS (~980 bp); ii) TRECOBF/TRECOBR PN D FJN D R thu thu on gen cob (~650 bp); iii) o n gen nad1 (~400 bp); iv) JB3F JB4 5R thu o n gen cox (444 bp), giải trình tự Các chu i c sử dụng pháp thi t số gen c mồi c hiệu h i iểm cho phản ứng PCR L-PCR Một mức ộ bảo tồn cao Gly nucleotide tRN ể thẩm nh loài danh 6S (rrnL) hay 12S (rrnS) hay chu i nh v sau gen nad5 c sử dụng thi t mồi l n lư t UNI 6F UNI 6R (trên rrnL), UNI 2F UNI 2R (trên rrnS), GLYF GLYR (trên tRNA Gly ) cho phản ứng L-PCR qua v ng m h a hông m h a mtDN (Bảng PL2 ) ể thực PCR thu nhận mtDN mồi chung ( mồi) sản phẩm PCR chu i H taichui, Bảng PL2 c hiệu ( mồi) c thi tvà sử dụng ch thư c hác Trên c ể thu s trình tự nucleotide thu nhận, ti p tục thi tmồi giải trình tự phư ng pháp lồng vào nhau, hay g liệt ê t t ti p i phư ng pháp “l o m ” trực ti p từ sản phẩm ho c sau hi tách dòng Giải trình tự c thực t i c s d ch vụ: Macrogen (Hàn Quốc) t i (https://www.macrogen.com/en/main/); Công ty Nam Khoa (Việt Nam): (http://biotechem.com.vn/) PL2.3 P ương p áp t ế t ế m g tn t ơn v m oso m ể thực PCR thu nhận rTU H taichui H pumilio Việt Nam, Bảng PL2 liệt ê t t mồi c sử dụng ể thu sản phẩm PCR ch 14 thư c hác Trên c s trình tự nucleotide chu i thu nhận, ti p tục thi t mồi giải trình tự phư ng pháp ti p lồng vào nhau, hay g i phư ng pháp “l o m Giải trình tự ” trực ti p từ sản phẩm ho c sau c thực t i c s hi tách dòng d ch vụ: Macrogen (Hàn Quốc) t i (https://www.macrogen.com/en/main/); Công ty Nam Khoa (Việt Nam): (http://biotechem.com.vn/) Mồi sử dụng ể thu nhận rTU H taichui H pumilio Việt Nam (Bảng PL2 4) cthừa từ nghiên cứu trư dựa số công bố v ch ng công bố rTU c y c thi t bổ sung c Ng n hàng gen ho c d ang c (Le et al., 2017; 2020b) ho c liệu c sưu t m ể t h p sử dụng (Olson et al., 2003; Tkach et al., 2016) PCR LPCR dụng phối h p c p mồi c áp hác lệch ể thu ph n DN (ph n o n) dài ng n hác Toàn m i c bi t cho n v rTU (5 n c 8S-ITS -5 8S-ITS2-28S-IGS ) sán lá, ộ dài hoảng từ 7–8 cho loài Ph n m h a (coding region) c t nh từ n 9– u gen u gen 28S, ộ dài thư ng hoảng 6–7 ho c Các gen 8S, 8S, 28S rRN ch h c c ộ dài cố nh Tuy nhiên, v ng ITS- (gi ITS-2 (gi a gen 8S gen 28S) v ng l nhi u c u tr c l p li n c số lư ng C số mẫu thu b, t y thuộc m i loài chi, 8S gen 8S) số loài c hác nhau, nên tổng c giải trình tự nht b, dài ng n hác a gen IGS, 8S cho thể c ch thư c s hác c toàn ph n m h a, hông thu c v ng l (IGS) PL2.4 n p np n ứng t nP v mt snp m Phản ứng PCR c thể i u chỉnh thành ph n chu trình nhiệt ph thu sản phẩm PCR tốt nh t c thể thu nhận ph n rTU Ch ng gi i thiệu phản ứng c Phản ứng c tổng thể t ch o n mtDN hiệu su t tốt nh t (Thermo pmol µL), µL hn DN (25–5 ng µL), µL DMSO (dimethyl sulfo ide) µL nư c ion DEPC, c thực máy MJ PTC-(US ) Chu trình nhiệt gồm: chu ỳ 15 c thực µL, gồm: 25 µL PCR mastermi Fisher Scientific, MA, USA), µL m i lo i mồi ( h p ể o 95 C ph t, 35 chu o ỳ o o ỳ [94 C/1 phút, 52 C/1 phút; 72 C/2–6 ph t], chu o cuối 72 C/10 phút Phản ứng L-PCR c thực v i en yme Phusion DN polymerase (Thermo Fisher Scientific Inc ), sản phẩm thu cc ộ dài 4–6 b v i ch t lư ng tốt Sản phẩm PCR LPCR thu c c iện di iểm tra th ch agarose % nhuộm ethidium bromide, so sánh ộ dài v i th DN Sản phẩm PCR n b ng c tinh s ch trực ti p sinh phẩm GeneJET PCR Purification Kit (Thermo Scientific Inc ) DN Lamda (HindIII) ng ch thư c dự t nh V i sản phẩm a b ng, b ng c ph n lập từ th ch agarose sinh phẩm GeneJET Gel Extraction Kit (Thermo Scientific Inc.) Các sản phẩm PCR tinh s ch sinh phẩm GeneJET giải trình tự t i c s d ch vụ PL2.5 P ương p áp tá PCR Purification Kit c gửi i gi i thiệu dòng t u DNA pl sm d tá tổ ợp Sản phẩm PCR ho c c giải trình tự trực ti p, n u c hàm lư ng ủ theo yêu c u Một số sản phẩm cloning thư ng m i ch t lư ng tốt c tách dòng sử dụng vector T pCR2 TOPO (Invitrogen) ho c TOPO Cloning Kit (Invitrogen Inc) (n u sản phẩm PCR
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