Nghiên cứu đặc điểm hệ gen ty thể và đơn vị mã hóa ribosome của một số loài sán lá ruột thuộc họ heterophyidae ký sinh gây bệnh trên người và động vật tại việt nam

VIỆN HÀN LÂM KHOA HỌC VÀ CÔNG NGHỆ VIỆT NAM VIỆN CÔNG NGHỆ SINH HỌC LÊ THỊ VIỆT HÀ NGHIÊN CỨU C I M HỆ GEN TY TH VÀ N VỊ M H RI OSOME CỦ MỘT S LOÀI SÁN LÁ RUỘT THUỘC HỌ HETEROPHYIDAE KÝ SINH GÂY ỆNH TRÊN NGƯỜI VÀ ỘNG VẬT TẠI VIỆT N M Chuyên ngành: Hóa sinh học Mã số: 42 01 16 LUẬN ÁN TIẾN SĨ SINH HỌC Người hướng dẫn khoa học: GS.TS Lê Thanh Hịa Viện Cơng nghệ sinh học PGS.TS ồng Văn Quyền Viện Công nghệ sinh học Hà Nội, 2021 i M ƠN t rưở Ph C t s us hất đ M ễ dị h, V ệ C hệ V ệt Nam, th a tru hư v pđ t t t ,đ v hệ s h họ , V ệ H o th dành h u th GS.TS Lê Thanh Hoà, nguyên s t m hu t đ t đ t t h tr th , t o mọ đ u tro qu tr h m Khoa họ v t p hư ệ thu h d , ợ , trao đ , đị h u, họ t p v ho th h u V ệ trưở Nam, th v Vệ C đồ h s us đ PGS.TS Đồ hệ s h họ , V ệ H hư d hoa họ đ t Vă Qu , Phó V ệ m Khoa họ v C t h hỉ ảo t tro hệ V ệt th a họ t p h u Phòng u ảm h M ễ dị h họ - V ệ C V ệt Nam đ - Ba t p thể hệ s h họ - V ệ H h ệt t h đượ th h hất t pđ t ảm ho th h u hđ oVệ C m h ệ hệ s h họ , V ệ H ho t Quố B h, Chủ tị h H đồ rư Phòng, Khoa Họ v ệ đ t o đ u s hỗ trợ m Khoa họ v C đượ họ t p v - Ba G m đố Họ v ệ Y–Dượ họ a (NAFOS ED) tro hệ : hệ V ệt Nam đ t o đ u -C m m Khoa họ v C h tru v V ệt Nam, PGS S Ph m , đồ ệ pđ t u tro h ệp B m , suốt qu tr h họ t p h phí Quỹ Ph t tr ể Khoa họ v đ t m số - hệ Quố v 108.02-2017.09 GS S h H a hủ h ệm trùng-C th hệ u S Đỗ ru Dũ , tr ru v rưở v ệ thu m u, thí Khoa K s h tr , V ệ Sốt r t-Ký sinh đồ h ệp Khoa đ h ệm h u h h th pđ t h họ cho nghiên ii Cuố u tru u t t hu hí h, đ h ệt hu t pt t v ho , pđ s us ũ đ th , hư h a s th h qu tr h họ t p v h h ađ hđ h hă v u m h ê hị V ệt Hà iii LỜI CAM KẾT Tôi xin cam đoan: Đây cơng trình nghiên cứu tơi số kết cộng tác với cộng khác; Các số liệu kết trình bày luận án trung thực, phần công bố tạp chí khoa học chuyên ngành với đồng ý cho phép đồng tác giả; Phần cịn lại chưa cơng bố cơng trình khác Hà Nội, ngày tháng Tác giả năm 2021 iv MỤC LỤC Trang Lời cảm ơn i Lời cam kết iii Mục lục iv Danh mục chữ viết tắt ix Danh mục bảng xii Danh mục hình xiv MỞ ĐẦU CHƯƠNG TỔNG QUAN TÀI LIỆU 1 Tình hình nghiên cứu sán ruột nhỏ h Heterophyidae thuộc lớp Sán Trematoda 1.1.1 Phân loại sán ruột nhỏ họ Heterophyidae 1.1.2 Đặc điểm sinh học phân b c a sán ruột nhỏ họ Heterophyidae 1.1.3 Tình hình nghiên cứu sán ruột nhỏ họ Heterophyidae gi i v Việt Nam 11 1.2 T th , ribosome t th v 17 1.2.1 Gi i thiệu ty thể c a động vật 17 1.2.2 c i m h gen t th ibosome c a ty thể 21 1.2.3 Cấu trúc v xếp gen hệ gen ty thể 21 1.2.4 Đặc điểm gen v v ng kh ng m h a hệ gen ty thể 23 1.2.5 Nghiên cứu ứng dụng gen v hệ gen ty thể 29 1.3 Giới thi u Ribosome c a t n v m h a ri osome 31 1.3.1 Gi i thiệu ribosome c a tế b o 31 1.3.2 Đơn v m h a ribosome 33 1.3.3 Nghiên cứu ứng dụng ch th phân t ov c a đơn v m ribosome h a 37 v 1.4 ngh a v s c n thi t nghiên cứu c i m mtDNA v v m h a ri osome c a sán ruột nhỏ Haplorchis c a CHƯƠNG n t i 40 Đ I TƯ NG VẬT LIỆU VÀ PHƯƠNG PHÁP NGHIÊN CỨU ……………………………………………………………… 43 2.1 Đ it ng v t li u a i m nghiên cứu ……………………… 43 2.1.1 Đ it ng v vật liệu nghiên cứu ………………………………… 43 2.1.2 M u nghiên cứu c a lo i thuộc họ Heterophyidae ………… 43 2.1.3 Đ a điểm v thời gian nghiên cứu 45 2.2 Ph ng pháp ti p c n v tr nghiên cứu …………………… 45 2.3 Ph ng pháp nghiên cứu h gen t th ……………………… 47 2.3.1 Ph ơng pháp xác đ nh cấu trúc v xếp hệ gen ty thể c a sán ruột nhỏ Haplorchis taichui ……………………………………… 2.3.2 Ph ơng pháp phân t ch đặc điểm gen N ribosome (MRG) ty thể ………………………………………………………………… 2.3.3 47 Ph ơng pháp phân t ch đặc điểm gen N vận chuyển t N 48 ty thể ………………………………………………………………… 48 2.3.4 Ph ơng pháp phân t ch v ng kh ng m h a hệ gen ty thể …… 49 2.3.5 Phân t ch ph ơng thức s dụng nucleotide v t nh toán giá tr độ lệch skew skewness hệ gen ty thể ……… ……………… 2.3.6 Phân tích đặc điểm gen m h a protein v s thiên v s dụng m …………………………………………………………… 2.3.7 2.4 51 Ph ơng pháp phân t ch phả hệ d a ch th cộng h p ba gen PCG c a ty thể (cob+nad1+cox1) ……………………………… 2.3.10 50 Ph ơng pháp t nh toán khoảng cách di truy n c a lo i sán ruột nhỏ d a cộng h p 12 PCG c a ty thể ………………… 2.3.9 50 Ph ơng pháp t nh toán khoảng cách di truy n c a lo i sán ruột nhỏ d a cộng h p gen cob+nad1+cox1 ……………… 2.3.8 49 51 Ph ơng pháp phân t ch phả hệ d a ch th cộng h p to n 12 PCG c a ty thể ……………………………………………… 52 Ph 53 ng pháp nghiên cứu n v mã hóa ribosome (rTU) vi 2.4.1 Ph ơng pháp xác đ nh cấu trúc v xếp gen v ng giao gen c a đơn v m h a ribosome ………………………………………… 2.4.2 Ph ơng pháp xác đ nh độ d i v đặc điểm gen r N v v ng giao gen đơn v m h a ribosome 2.4.3 skew đơn v m h a ribosome …… 57 CHƯƠNG KẾT QUẢ NGHIÊN CỨU ………………………………… 58 K t qu nghiên cứu h gen t th loài Haplorchis taichui m u Vi t Nam ………………… 3.1.1 56 Ph ơng pháp phân tích phả hệ c a H taichui v H pumilio d a cộng h p gen (18S+28S rRNA) to n ph n 3.1 56 Ph ơng pháp phân tích phả hệ c a H taichui v H pumilio d a chu i gen 28S rRNA to n ph n …………………………… 2.4.8 55 Ph ơng pháp phân tích đặc điểm cấu trúc bậc hai v ng giao gen ITS (ITS-1, ITS-2) ……………………………………………… 2.4.7 54 Ph ơng pháp phân tích đặc điểm cấu trúc bậc hai gen r N ribosome (5.8S, 18S, 28S rRNA) ………………………………… 2.4.6 54 Ph ơng pháp phân tích t ơng đ ng nucleotide c a gen r N v ph n m h a ribosome c a sán ruột nhỏ họ eterophyidae 2.4.5 53 Ph ơng pháp phân t ch ph ơng thức s dụng nucleotide tính tốn giá tr độ lệch 2.4.4 53 58 Cấu trúc v xếp hệ gen ty thể c a sán ruột nhỏ Haplorchis taichui 58 3.1.2 Phân t ch đặc điểm gen N ribosome c a ty thể ……………… 62 3.1.3 Đặc điểm gen N vận chuyển t N 63 3.1.4 Đặc điểm v ng kh ng m h a mtDNA c a sán ruột nhỏ c a ty thể ……………… Haplorchis taichui ……………………………………………… 3.1.5 Ph ơng thức s dụng nucleotide v giá tr độ lệch skew hệ gen ty thể ……………………………………………………… 3.1.6 Phân t ch đặc điểm gen m h a protein v thiên v 65 65 s dụng m (codon usage) ………………………………………………… 66 vii 3.1.7 Khoảng cách di truy n Haplorchis taichui v lo i sán khác họ eterophyidae v Opisthorchiidae d a cộng h p amino acid c a ba gen (cob+nad1+cox1) ……………………… 3.1.8 68 Khoảng cách di truy n Haplorchis taichui v lo i sán khác họ eterophyidae v Opisthorchiidae d a cộng h p amino acid c a 12 PCG ………………………………………… 3.1.9 Phân t ch phả hệ sán ruột nhỏ d a chu i amino acid cộng h p c a ba gen m h a protein (cob+nad1+cox1) ……………… 3.1.10 K t qu nghiên cứu 77 Cấu trúc v xếp gen c a đơn v mã hóa ribosome c a Haplorchis taichui 3.2.2 74 n v m h a ri osome loài Haplorchis taichui v lo i H pumilio m u Vi t Nam ……………………… 3.2.1 72 Phân t ch phả hệ sán ruột nhỏ d a chu i amino acid cộng h p c a to n 12 gen m h a protein ………………………… 3.2 70 ………………………………… 77 Độ d i gen r N v v ng giao gen đơn v m h a ribosome c a lo i Haplorchis taichui v Haplorchis pumilio 80 3.2.3 Đặc điểm cấu trúc bậc hai gen r N … 81 3.2.4 3.2.5 Đặc điểm cấu trúc v ng giao gen T T -1, ITS-2) …………… ết phân t ch ph ơng thức s dụng nucleotide đơn v 86 m h a ribosome ………………………………………………… 88 3.2.6 r N ết phân t ch t ơng đ ng nucleotide c a gen r N v ph n m h a ribosome c a sán ruột nhỏ họ eterophyidae … 3.2.7 Phân t ch phả hệ c a H taichui v H pumilio d a chu i nucleotide gen 3.2.8 90 ribosome to n ph n ………………………… 91 Phân t ch phả hệ c a H taichui v H pumilio d a cộng h p to n ph n hai gen rRNA (18S+28S rRNA) ……………………… 94 CHƯƠNG BÀN LUẬN KẾT QUẢ …………………………………… 97 V nghiên cứu h gen t th sán ruột nhỏ Haplorchis taichui 99 4.1 viii 4.1.1 V thu nhận liệu gen học hệ gen ty thể ……………………… 99 4.1.2 V đặc điểm hệ gen ty thể c a sán ruột nhỏ Haplorchis taichui 100 4.1.3 V s ứng dụng mtDN nghiên cứu di truy n v phả 4.1.4 hệ ………………………………………………………………… V đ ng g p cung cấp sở liệu hệ gen ty thể ……………… 4.2 V nghiên cứu 104 107 n v m h a ri osome H.taichui v H pumilio …………………………………………………………… 109 4.2.1 V thu nhận liệu gen học đơn v m h a ribosome …………… 109 4.2.2 V đặc điểm đơn v m h a ribosome c a sán ruột nhỏ Haplorchis taichui v H pumilio ………………………………… 110 4.2.3 V s ứng dụng rTU nghiên cứu di truy n v phả hệ … 113 4.2.4 V đ ng g p cung cấp sở liệu đơn v m h a ribosome … 116 KẾT LUẬN VÀ KIẾN NGHỊ 119 NHỮNG CƠNG TRÌNH ĐÃ CƠNG B LIÊN QUAN ĐẾN ĐỀ TÀI 121 TÓM TẮT BẰNG TIẾNG ANH 122 TÀI LIỆU THAM KHẢO 130 PHỤ LỤC ix DANH MỤC CÁC KÝ HIỆU, CÁC CHỮ VIẾT TẮT Chữ viết tắt Nội dung i i ngh ATP Adenosine triphosphate Phân tử mang lượng bp Base pair Cặp bazơ Chu i chung l t ch nh nhi u chu i cộng Consensus sequence cs DNA Deoxyribonucleic acid Axit Deoxyribonucleic ĐVLSN Zoonosis/zoonoses Động vật lâ sang người người khác et al ETS hai pin /stem External Transcribed Spacer Vùng ngoại gen Secondary structure C u t c nhánh nh thái h c HTH Morphology IGS Vùng l ITS Non-transcribed intergenic spacer Integrated Taxonomic Information System Internal Transcribed Spacer Kb (= Kbp) Kilo base pair Đơn vị đo (= 1.000 cặp bazơ) KCDT Genetic distance KST Parasite LNR Long non-coding region Vùng không mã hóa dài Secondary structure C ut c Long- Polymerase Chain Reaction Phản ứng chu i t ùng hợp dài ITIS loop LPCR a th ng th ng tin phân loại t ch hợp Vùng giao gen hoảng cách di t u n sinh t ùng nh nh i n gi i g n ùng gi o g n củ mtDNA hoàn ch nh ( 5.120 bp) oài Haplorchis taichui củ iệ N (chủng Htai-QT3-VN/GenBank: MG972809) cox3(645) * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 HtaiQT3-mt : ATGAGGTGGTTACCTGTTTATAATTCTTGAGTGGTTATGGTGGGTATAGTAAGTTTGTTTTTGTGAAAGCTTTGGGGCGTTTTTTTCTTTTTGGGTGCGTTGATTTTTTCGGCTTATTTTCTTGTATGAGAGGTTTTTTTTCCACATTTAAGGCGGTATGCTATGGCTTTTTGACTATTT : M S W L P V Y N S W V V M V G I V S L F L W K L W G V F F F L G A L I F S A Y F L V W E V F F P H L S R Y A M A F W L F * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 HtaiQT3-mt : ATAGTTAGTGAGGTGGCTATTTTTGGGAGGTTGCTTTTAACTGTTCTTTGAAAAGATGACGGTGGGGAGGGGGCCTTGTCGGATAGCTTGGAGTTGCCTTTTTTAGGGTGTTTTCTTTTGTTGACTTCCTCTTTGACGGCCACAGTGTATCATCATTGTTTTGGCTTGCCTTATAGTTGG : I V S E V A I F G S L L L T V L W N D D G G E G A L S D S L E L P F L G C F L L L T S S L T A T V Y H H C F G L P Y S W * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 HtaiQT3-mt : GTTTTTTTACTTATCAGTATCTTTTTAGGGTTTGGGTTTGTGTGTCTGCAGTTGTGGGAGTTTTTTGATTGTGAGTGTGATGTTCTTTATTGTACTTATTTTGGGGCTGCTTTCTGTACTGTTGGGTTGCATTTTAGTCATGTGGTTGCGGGGGTCGCTGCTATGACCGTTTTGTTAGTG : V F L L I S I F L G F G F V C L Q L W E F F D C E C D V L Y C T Y F G A A F C T V G L H F S H V V A G V A A M T V L L V trnH(His)(66) 180 360 540 cob(1110) * 560 * 580 * 600 * 620 * 640 * 660 * 680 * 700 * 720 HtaiQT3-mt : AGGGGTTGGGATACCAATCATTTTTATGCTAGGGTGGTTGTGTGGTATTGGCATTTTGTTGATTATGTGTGGTTGTGAGTATACCTGTTAATATATTACGTTTAGGGTCTTCAATAGGTTATTTAAAACTGTTGTCTTGTGGAGACAGAGATTCGTTGTGTGTGATTGAGGAAGGTTTAA S G W D T N H F Y A S V V V W Y W H F V D Y V W L W V Y L L I Y Y V * * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 HtaiQT3-mt : TGGTTTCTGTTGTTCGTGGTAAAGTAGTGGATCTTCCTACTAAAGTTTCTTTGAGGTATTTTTGATGTGGGGGGTTTATGATTAGTGTGTTTTTAGTCATTCAGGTTGTATCTGGTGTAATTCTGTCTTTGCTTTATGTAGGAGATGCGGGGGTGAGGTTCGGGTTGGTGCTGGATTTTG M V S V V R G N V V D L P T N V S L S Y F W C G G F M I S V F L V I Q V V S G V I L S L L Y V G D A G V S F G L V L D F * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 HtaiQT3-mt : TTAACGAGAGTTTGTTTTTATGATTTGTCCGTTATTTGCATATATGGGGAGTAACGTTTATTTTCCTACTGTTCTTGGTTCACATGGGGCGTTCTTTTTATTATTCTAGTTATAGGAAGTTGGGGGTATGAAAAGTTGGGTTCGTTCTTTATATTTTAATGATGATTGAGGCGTTTTTAG V N E S L F L W F V R Y L H I W G V T F I F L L F L V H M G R S F Y Y S S Y S K L G V W N V G F V L Y I L M M I E A F L * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 HtaiQT3-mt : GGTATATTCTTCCGTGACATCAGATGTCCTATTGGGCTGCCACTGTTTTAACCTCGATACTTAAAAGTATACCAGTTGTAGGCGGTAAGCTTTATGAGTTTATTGTTGGGGGCTTTGGGGTCACTAAAGTGACTCTAGTCCGTGTTTTTTCAGCTCATGTGTGCCTGGCTTTTGTGATAA G Y I L P W H Q M S Y W A A T V L T S I L N S I P V V G G K L Y E F I V G G F G V T N V T L V R V F S A H V C L A F V I * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 HtaiQT3-mt : TTGGGCTGAGTGTGATTCATCTTTTTTATTTGCATAAGGGAGGGTCTAATAATCCTTTATCTGCGCCTGGGGGCTACAGGGACGTTGTCCTGTTTCATTCATATTTCACTAGAAAGGATGGGGCGGTGCTTGTATTATTGTTATTACTCCTGGGAGGAGGGTTGATGATATCGCCAGACG I G L S V I H L F Y L H K G G S N N P L S A P G G Y S D V V L F H S Y F T S K D G A V L V L L L L L L G G G L M I S P D * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 HtaiQT3-mt : CTGTTCTAGATGTGGAAAAGTATATTGAGGCGGATCCGATGGTTACTCCTGTGTCAATAAAGCCTGAGTGGTATTTTCTTGCTTTTTATGCGATGCTGCGGTCTATAGAGTCGAAGGTTGGGGGCTTGGTGCTGGTTTTGATATTCCTGTTCGTTTTGTGATTACCTACTTTTAATTCCT A V L D V E K Y I E A D P M V T P V S I K P E W Y F L A F Y A M L R S I E S K V G G L V L V L I F L F V L W L P T F N S * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 HtaiQT3-mt : CTTGTGTTTATAGTGTGTTGCGTCAGTATGTATTTTGGGGCTGTTGTTCTCTTTACTTCATTTTGAGCTATCTTGGTGCTTGTCACCCCGAATATCCTTTTGTTTTTATAAGCAAGTTTTGTTCTCTCTTGATTGTTGTATTATTAATGCTGTTTAAGGGGCTGTGGGCAGTTCCTTATT S C V Y S V L R Q Y V F W G C C S L Y F I L S Y L G A C H P E Y P F V F I S K F C S L L I V V L L M L F K G L W A V P Y : 720 : 900 : 1080 : 1260 : 1440 : 1620 : 1800 * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 HtaiQT3-mt : CTGGGGGGTTTCCTCGTTTGGTTTTGTAGTATGGTTCGGTTGCTTTTGCTTTTAGGCATTTTTATAATTTTGTGTAGGTTTTTTCTTTCTTTGACGCGCTTGTTGAAATGCCTAATAGTGGTTGAGAATTTTAAAGTGTTGGTGTTATTTGTGAGTTTATTATCCCAGCGGGGTGAGACT : S G G F P R L V L * M V R L L L L L G I F I I L C S F F L S L T R L L N C L I V V E N F N V L V L F V S L L S Q R G E T 1980 nad4L(264) nad4(1281) (40 bp overlapping with 3’ end of nad4L) HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : * 2000 * 2020 * 2040 * 2060 * 2080 * 2100 * 2120 * 2140 * 2160 TTTATACTTTTCATTGCCTTGATGGTGATTTTTACTATTGAGGTTATTCTTGGTTTGGTGGTTTTGACCCGGTTGTGGGATTCAAGAGGTTTAGTTGGTATAGTGGGTTGATAGGGGCCTTGTCGGTTAGTGGTTTGATTATTTTGGTTGAGGCCTGTGCAGGGTTGGATAGAATCTGGC F I L F I A L M V I F T I E V I L G L V V L T R L W D S S G L V G I V G W * V G F K S F S W Y S G L I G A L S V S G L I I L V E A C A G L D S I W * 2180 * 2200 * 2220 * 2240 * 2260 * 2280 * 2300 * 2320 * 2340 TTAGCCATTCTGGGGTTACGGCGGGAGTTTTTTGTTTTGATGTTATAAGCTTTTATCTGATTCTGCTTTCAATTTTATTATCCTTGTCTTTAAGTTTTTGGGTTGAGAGTATGAGATATTTTAGACGCTTTATGATTTTCTTGAGAGTGCTAGGGTCGCTTTTATGTTACTGTTGTGTTC L S H S G V T A G V F C F D V I S F Y L I L L S I L L S L S L S F W A E S M S Y F S R F M I F L S V L G S L L C C C C V * 2360 * 2380 * 2400 * 2420 * 2440 * 2460 * 2480 * 2500 * 2520 ATGTCGTTTGGTTTTGAGTTTTTTATGAGATGTCAATAGTTCCGTTGCTTGTGTTGTTAATTGTAGATTCTCCTTATTCGGAGCGTTATGTTGCTTCTTGATATCTTTTGGGGTATGTGGTTTTTACGAGTCTCCCAATGTTGCTGTGTATACTTTATTTGTCTCTACTTGAGGGGGGGT H V V W F W V F Y E M S I V P L L V L L I V D S P Y S E R Y V A S W Y L L G Y V V F T S L P M L L C I L Y L S L L E G G * 2540 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660 * 2680 * 2700 TTAATATGGGGTGTTGGGGGTCTTCTGATAGGTGGGTTGTAACCGGGAGGTTAATTGTTTTAGCCTTTCTTTTTATTACAAAGATACCTTTGCCTCCGTTTCATGTGTGGCTTCCGATAGTTCATGCTGAGGCTAGGAGCCCAGTATCTGTCTGTTTGAGGGGGTATATAATGAAGTTGG F N M G C W G S S D S W V V T G S L I V L A F L F I T K I P L P P F H V W L P I V H A E A S S P V S V C L S G Y I M K L * 2720 * 2740 * 2760 * 2780 * 2800 * 2820 * 2840 * 2860 * 2880 GTTTACTTGGTGTCTGCCGGTTTTGTTTTTTTTTACTTCCCGATTTTATTTTCTCTTTGGCTTATACGGTTATATGCTTTTTATGTTCTGTGCTCTTCTTCTTTTCTGCTAGTCGGGAGTTGGATGGTAAGCGCTGACTTGCTTTTTTAAGTCTTTCACACATTTTAGTGGCTATGGGCT G L L G V C R F C F F L L P D F I F S L A Y T V I C F L C S V L F F F S A S R E L D G K R W L A F L S L S H I L V A M G * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 * 3060 GTCTGTCTGTGGGGGGTTGAGTATTGAGGGGCTCAGCTATTTTGTATTGTATGGGTCATGGGCTGTCTGCGGGGGTGATGTTTTTATTTCTTTGGTTGATGTATGATGTGGTGGCTACTCGTAATTTAGCTTTGTTGAAGTCGGGGATTTCGGGAAGTTTGTTGTTTCGTGTGTTGGCTT C L S V G G W V L S G S A I L Y C M G H G L S A G V M F L F L W L M Y D V V A T R N L A L L K S G I S G S L L F R V L A * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 GTTGCTGTTTATGTACTGCTTGTTCTTTGCCCCCCACAGTACAATTTTTTTGTGAAGTTTCGTTAATGACTGATGGGGCCTGGAGGGGGGGTTTCTTTTTACTAGTTTTTTATGCTTATGTCTTTATGGGAGGCTTGATGCCTTTATTTTTAGTAGGAAGGTTATTGAGCCGGCATTATG C C C L C T A C S L P P T V Q F F C E V S L M T D G A W S G G F F L L V F Y A Y V F M G G L M P L F L V G S L L S R H Y trnQ(Gln)(66) : 2160 : 2340 : 2520 : 2700 : 2880 : 3060 : 3240 trnF(Phe)(64) * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 * 3380 * 3400 * 3420 HtaiQT3-mt : ATGTGAGGTTTCATGGGGGTTCAGCAATCGGTGTAAAGGTTTATTCTTTATTCTACTTGATTATGTGGAGGTTTGGCTTATTTCTGGTATGTTAAATTGGGGTTTATAGCTTGGTGTAGTTAGAAGCATGCTATGTTTTGGTTGTAGCGGGGATTGGATGTTAGCTATAGTGTTTCCGCC : D V S F H G G S A I G V K V Y S L F Y L I M W S F G L F L V C * trnM(Met)(64)(1 bp overlapping with trnF) 3420 atp6(516) * 3440 * 3460 * 3480 * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 HtaiQT3-mt : TAGCTTAAATTTAAAGCGTTAGTTTGAAGAGCTGAAGATATTATATTGTGGTGGGAGGCGGGATAAGTTAAGTGGGAAACTCTATGGTTCATGTTCATAAAATGCGTGTAGCTCCTGCCTATGTGTTTTTCCCGTGTGAGAAGAGTTTATAATAATGTGATTAGATATGTGGCGGGGGGA : M C F S R V S S V Y N N V I S Y V A G G * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 * 3780 HtaiQT3-mt : TTGGGAGATAGATTTTATCGTTTACTTCTTTTTATGGTGTTGTTAGTTTTTTTGGGGTTACGGCTTCCTTATTTTTATGGGATGGGGGGATTTGGTATTTTTATTTTGATTAGGGTTTTCCCCCTTTTTATGAGTTTGTTTTTTTCTCGTTTGATTGATGGTAGTATGAGTGCGTTTTTT : L G D S F Y R L L L F M V L L V F L G L R L P Y F Y G M G G F G I F I L I S V F P L F M S L F F S R L I D G S M S A F F * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 HtaiQT3-mt : TGTGGCTTTGTTCCTCAGGGTACTCCGTTGTGGATAGCTCCGTTTGTTTGTTTGGCTGAAACTCTAAGTTATGTTGTGCGGCCCGTGGTTTTGATGATACGTCCTTTTGTAAATCTGAGTATTGGAGCTTTAGGGGGGGCAGTATTGGGTGCTTTAAGTATGGCTAGAGGTTGAGTTTTA : C G F V P Q G T P L W I A P F V C L A E T L S Y V V R P V V L M I R P F V N L S I G A L G G A V L G A L S M A S G W V L 3600 3780 3960 nad2(870) * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 HtaiQT3-mt : TTTTTTTTGGTTCTTTTATTTTTTTATGAGGTCTTTGTCGCGTTGGTTCATTGATTTATCGTGTGCAGTATCCTTTCTTTTTCGGAGGATCACTAGAAGGGGGGGGGGTAATCGGGCTTAGATGCGCGGGCTGGCGTTATCTGGTTTTAGGGTGGTCAGTGTTTTCTTGTTCTCTTTATG F F L V L L F F Y E V F V A L V H W F I V C S I L S F S E D H * M R G L A L S G F S V V S V F L F S L C * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 HtaiQT3-mt : CTTGTTCTTGAGAGATAATCTTTTATTATTCTGGCTGTTTTTGGAACTTGGCGCTTTGAGGTTGGTTCCTGGATTTTTTATAGTTGGTGGGAGGGGCGCCTTGGGCGGTCTTTTTAGCTACATTTTGGTTTCTGCGGTTTCTTCTTCGTTAATAGTAAGGGGACTGTTATACGAGGATGT L F L S D N L L L F W L F L E L G A L S L V P G F F I V G G S G A L G G L F S Y I L V S A V S S S L I V S G L L Y E D V * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 HtaiQT3-mt : TTTTTTTCTGTCTTGTTTGGGATTGATAATGAAGTTTGGGGTTTTCCCCTTTATGGGTTGAGTATATGTTGTTGTTTTGGCTAGCAACTGGTTAGTAATATGATTGCTTTCGACTGCTTTAAAGATGGGTTTTTTCTTTTTTTGTTTTTTTATTAGGAGGGGGTGAGATTCTTTGCTTGT F F L S C L G L I M K F G V F P F M G W V Y V V V L A S N W L V I W L L S T A L K M G F F F F C F F I S S G W D S L L V * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 HtaiQT3-mt : AATGATGTTGTGTGTGTTTACCTTTTTGGTGTTGAGTCTGTTGTTTTGGGTTTTTACTCCTAGTTGACTGGTTTATTGGTGTCATGTCTTGATTAGATCTAGGGCTTCTTTGATACTAATGGCTTTGGAGGTTTCAGTTGATTTACTTTTATGACTGTTTGTTATTTATTTTGTGTGGTC M M L C V F T F L V L S L L F W V F T P S W L V Y W C H V L I S S S A S L I L M A L E V S V D L L L W L F V I Y F V W S * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 HtaiQT3-mt : TACTTGTGCTATTATGCTTTTACGTAGGCTTAGCTTGATGAGCCTGGGTAATAGCGTGGTGGTTTTTTGGGTCGTGTTTATTTTGGTGGCGTTTCCTTTCTCTGTTTCAATTTTTTATAAGTTGGTTGTTAGTCTTTGTATGTACTCGTGTGGTGTTTTAGCATTGTTGGGGTGGGTTGT T C A I M L L R S L S L M S L G N S V V V F W V V F I L V A F P F S V S I F Y K L V V S L C M Y S C G V L A L L G W V V : 4140 : 4320 : 4500 : 4680 : 4860 * 4880 * 4900 * 4920 * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 HtaiQT3-mt : GTATAAAGTTTCGGAGCAGTTTTTTTTAGTTAAGTATATGGTGAGAGAAGGTATTCCCCGTGCGGAGTGAAGAGGAGTAGGTGTGGTTTAAGGGAGGCGAGATAGTTTATTGAAAATGTCTATTTTACGCGTAGAAGAAGGATTACCTATCGTCAGAACGATATAGTTTAATAGGAGATG : Y N V S E Q F F L V K Y M V S E G I P R A E W S G V G V V * 5040 trnV(Val)(60) trnD(Asp)(67) trnA(Ala)(63) nad1(906) * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 * 5180 * 5200 * 5220 HtaiQT3-mt : GCCGCTCTGCAAGCGGTCGGTGAAGGATTTCTATCGTTAGCCTGTTCTTAGTTTATTGTAATAATAGCTTGTCGTGCTATAGGAGGGTGGCTAGACACTCAGAGCGGGGTGACTTGGTTGTTGGGCGTTTATAATTTTCTTAGGTCGTTTTTGGGGTTTGCGGTGATAATGATTTTTGTA V T W L L G V Y N F L S S F L G F A V I M I F V * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 HtaiQT3-mt : GCCTTCTTTATTTTGGGGGAGCGCAAGGTTTTGGGTTATATGCAGATTCGTAAGGGTCCGAACAAGGTGGGGCTTGTAGGGCTTTTTCAAAGGTTTGCCGATTTGTTGAAGTTGATAATAAAGTTTAAGGTTAGCCTTTATCAGGTTCGTAGGTGACTCTCTTGAATTGGGGTTTATCTT A F F I L G E R K V L G Y M Q I R K G P N K V G L V G L F Q S F A D L L K L I I K F K V S L Y Q V R S W L S W I G V Y L * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 HtaiQT3-mt : TTGTTATTTTTGGCGCTTTGTTATTGTTTTGTTTTTGGGTTGTTTTATGGTGGTGTGAGTTGTGTAAAAGGTTTGCTTTGGGTATTGGTGGTAACGAGTATAACGGGGTATAGTCTTTTAAGCATTGGTTGAGGGTCTTATAAAAAGTATGCTTTGGTTAGTTGTGTCCGTTCTGCTTTT L L F L A L C Y C F V F G L F Y G G V S C V N G L L W V L V V T S I T G Y S L L S I G W G S Y N K Y A L V S C V R S A F * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 HtaiQT3-mt : GGGTCGGTGACTTTTGAGGCGTGTTTTATGTGTATTGTGGTGATTGTGGGTGTAGTCTGTGGGGGTTACTTTGGTTACTCGGTGATGGAGAATGCGTGGTTAGTCTTGTTGGTGAGACCTTTAGGATATGCTTTATGATTAGTGGGGATGTTATGTGAGTGTAAACGAACACCTCTAGAT G S V T F E A C F M C I V V I V G V V C G G Y F G Y S V M E N A W L V L L V S P L G Y A L W L V G M L C E C N R T P L D * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 HtaiQT3-mt : TATGCGGAAGCTGAGAGGGAGCTGGTGAGAGGGTTGAATACGGAGTATTGTAAAGTGCCTTTTACTTGCTTGTTTGCTTGTGAGTATCTGATAATGTTTATTTTTTCTTGGTTGGGGGCTTTATTATTTTGGGGAGGGGGCCTAATTTCGTTTTTTAGGTTTGTTCATGTTTTGTTTTTT Y A E A E S E L V S G L N T E Y C N V P F T C L F A C E Y L I M F I F S W L G A L L F W G G G L I S F F S F V H V L F F : 5220 : 5400 : 5580 : 5760 : 5940 * 5960 * 5980 * 6000 * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 HtaiQT3-mt : ATTTGGGCGCGTGCAACTTTACCTCGAGTGCGGTATGATTATTTTGTGGCTTTTATGTGAAAGTACTCAATCCTTATATTGGTTTTTTCGTTATTTTTAGTGGTGTTAGTTTAGTGGTGTGGGTAGATTAATGGTAAAATCGTTAAGCTGTTAACTTAAAGATGATCGGGTATCCTCAGA : I W A R A T L P R V R Y D Y F V A F M W K Y S I L I L V F S L F L V V L V * 6120 trnN(Asn)(66) trnP(Pro)(65) trnI(Ile)(65)(4 bp overlapping with trnP) trnK(Lys)(65) * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 * 6260 * 6280 * 6300 HtaiQT3-mt : CGAGGCAGTCGTATAGTCTATTTTAGGATGTTAGCTTTGGGGGCTTAAGGACTCTTGAGGAGTGGGCTGATAGGGCTGCTGAGCAGGCCACTGTGATATAGTGGTTGTAGGAGAATTAGATCCTCGTCGGTAGACTGTGGGTAGCTTAAGGTGTAAAGTGTTAGATTCTTACTCTAGTGA : 6300 nad3(360) * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 HtaiQT3-mt : TGTTTTTGAACTCCATAGGATGGGTTATTCTGGCTTGTGTGCTCTTTTATTCTTTTTGATTTTTTTTTTGGTCGGTGTATTCCATTTATTTTTATGGGATTCTGGTTTGTGAGGGTTTGTAAGTGGGTTTCGGTCTTGGGTCGGTAGGTTTGAGTGTGGTTTTATCTCTCAGGGGGTGTC : * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 HtaiQT3-mt : AGAGAAATATTTTAGGTGAACTTACTTTATACTGCTAGTCTTTTTTGTTGTTTTTGACTTGGAAATTTCGTTACTTCTAAAATTACCTTTACAAGGGCTGCTTTTTAAGAAATTGCCCTATTATGTGTGTTTTGtGGTACTCCTGTGCTTTGGTTTTGGGATTGAGGTGAATAAGGGTTA : trnS1(Ser1)(AGN)(62) trnW(Trp)(64) 6660 cox1(1542) * 6680 * 6700 * 6720 * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 HtaiQT3-mt : TGTCGAGTGAAGCTACTAGGGGTTAGATGTGTGAGAGCTATGTGAAGCTGTTGCTGCTAACAATGGTAAGAGTGTTTAAAGCGCTCGGCTCTCTTGTAGGGAACAATCTAGGTTAAGTAGAGACTGTCTGTCTTCAAAACAGAGGGTAGCTTGCGCTGTTTGTTGTTAGATGTTGACTTG : * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 * 7020 HtaiQT3-mt : ATTATTGACTTTGGATCATAAGCGAATAGGGTTGATATATATGATTGTTGGAGTGTGAGGGGGCTTTTTAGGTCTTTCTTTGAGGTTTTTGATCCGTGTTAACTACTTGGATCCTTACTATAATGTTGTATCCCCAGAGGTTTATAATTATGTAATTACTAGGCATGGTATTGCGATGAT : 6480 6840 7020 * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 HtaiQT3-mt : TTTTTTTTTCTTAATGCCGGTTTTGATTGGGGGGTTTGGAAAATATCTGTTGCCCCTGTTGTTGGGGCTTCCTGATTTGAATTTACCCCGTTTAAATGCCCTTAGAGCTTGGTTAATGATTCCTTCTGCTGTTTGTTTTGGGTTAAGTATGTTTGGGGGGTCTGGGGTTGGCTGGACTTT * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 * 7340 * 7360 * 7380 HtaiQT3-mt : TTATCCCCCCCTGTCTTCTAGGGATTATAGGGGTTGGGGTGTTGACTTTCTTATGTTTTCTTTACATTTGGCGGGGTTATCTAGTCTGTTTGGTTCTTTAAACTTTATTTGTACAATAATGAGGGCTATGAGTAATGATACCAGGGAGCGGTACTCTATTGTGGTTTGGGCTTATTTGTT * 7400 * 7420 * 7440 * 7460 * 7480 * 7500 * 7520 * 7540 * 7560 HtaiQT3-mt : TACTTCTATTCTTTTGTTGCTTTCGTTACCAGTGCTAGCTGCGGGTATAACGATGCTTTTGTTTGATCGTAGATTTAGCTCTTCTTTTTTTGATCCATTGGGAGGGGGGGACCCTGTCTTGTTCCAGCATATGTTTTGGTTTTTTGGGCACCCCGAAGTTTACGTTCTTATTCTCCCAGG * 7580 * 7600 * 7620 * 7640 * 7660 * 7680 * 7700 * 7720 * 7740 HtaiQT3-mt : GTTTGGTATAGTGAGACACATTTGTAGGACGTTAACAAATAAAGATTCCTTGTTTGGTTATGGGGGTTTAGTTCTTGCTATGTTTTCTATAGTCTGTTTGGGGAGTGTTGTTTGAGCTCATCATATGTTTATGGTTGGGTTGGATGTTAAGACGGCTGTTTTTTTTAGTTCTGTGACCAT * 7760 * 7780 * 7800 * 7820 * 7840 * 7860 * 7880 * 7900 * 7920 HtaiQT3-mt : GGTTATAGGAGTCCCCACAGGTATAAAGGTTTTTTCTTGGCTGTATATGTTGGCGGGAAGTCGGGGCCGGTTTTGGGATCCGATAATGTGGTGGATATTGGGTTTTATTGTCTTATTTACTATCGGGGGGGTGACCGGGATTGTGTTGTCTTCTTCTATAATGGATACTTTGTTGCATGA * 7940 * 7960 * 7980 * 8000 * 8020 * 8040 * 8060 * 8080 * 8100 HtaiQT3-mt : CACTTGATTTGTAATAGCTCATTTTCATTATGTTTTATCGTTAGGTTCTTATATGTCGGTGGTGATATCTATGATTTGATGATGGCCTGTGATAGTGGGTTATAGGTTGAAAAAGTACTTGTTGATGGCTCATTGGGGGGTGTCAATGGTTGGATTTAATTTGTGTTTTTTTCCTATGCA * 8120 * 8140 * 8160 * 8180 * 8200 * 8220 * 8240 * 8260 * 8280 HtaiQT3-mt : TTTTTTAGGGTTTCAGGGTTTACCTCGTCGGGTGTGTGTTTATGAGCCTTGTTTTCAGTGGTTGAAAACTATGGCTAGGCTGGGGGGACTTCTTTCTGTTTTTAGGGGGTTTATGTTAGCTTTCATTTTATGAGAGTCGTTGTCTGTGGGTAATCGGGTGATAGGGTTATGGGGTAGTAA trnT(Thr)(64) HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : HtaiQT3-mt : 7200 : 7380 : 7560 : 7740 : 7920 : 8100 : 8280 rrnL(980bp) * 8300 * 8320 * 8340 * 8360 * 8380 * 8400 * 8420 * 8440 * 8460 HtaiQT3-mt : AGCCTTGGTTTTAAAAGTAGTAACTTCTCCTGTGCCGCATCATTGTAGTTATATGAGTGAGCCGGTAACGTGGTTTTATCGTTGGTATTAGAGGGTGTATTTTAAGGAAGTAGAATGTAGCTTTTGTAAGGCTAAGGTGCTGGGGTCACCCTCTGGTTTATAGGCTGGTTAGGTGCTTAT * 8480 * 8500 * 8520 * 8540 * 8560 * 8580 * 8600 * 8620 * 8640 HtaiQT3-mt : TTCTGGGTTTATTTTACCTTTTGCATCATGATTCCTTGAGAGATTCCTTGTATAAGGGGTTCCGAAGTGCAGAGATTTCCTTTTGGTTAGCTTAGTGCTGTAGTTTTGGGCGGGTAGCCTTTGTTAAAGTCATTGGGGGAGGTGAAACATAAATCGTTCTGCTCGATATCTGATTTTTGC * 8660 * 8680 * 8700 * 8720 * 8740 * 8760 * 8780 * 8800 * 8820 HtaiQT3-mt : TAGGGTTTTCTTGGGATCCTTGTTGTTTGGAAATGATGGGGAGGGTAAGAGTCTTGCTGTGTTATTGATGGATTTAGGTTAGAGGTACCTTTTTGTTGTACTATGTGTTAAGACTGGGTAACTTAGCCTGTTGAGAGATACTTTGTTGGGGCTAGCAGAGGTTTAATTTTGTGTCGTGAG * 8840 * 8860 * 8880 * 8900 * 8920 * 8940 * 8960 * 8980 * 9000 HtaiQT3-mt : TATGAGGGAATAAGTAGTTTTATTAGCTCTCTCTTTTCTCGGGTGCCTCCGGTCTGTTTATTAAAAACATTTCCATTAGTAAGGATTAATGGTAGTACCTGCCCGATGTAATAATGAATGGCCGCAGTATCTTGACTGTGCTAAGGTAGCATAATTAGTTGCCTCGTAATTTGGGGATTG * 9020 * 9040 * 9060 * 9080 * 9100 * 9120 * 9140 * 9160 * 9180 HtaiQT3-mt : TTTGAATGGTTTGACTTGGGGGTTTATCTAGGTGGGGGCTTTGTTGCGAAATTGGTTGATCAGTGCAGAATCTGATCCTTTTTGATTAGACGGAAAGACCCCGAGAGCTTGCAGTAGGATGTTTTATCTGGGGAGGAGGCAAATGTTTCATTTGTTTTTAGACCCTTTTGGGTAAAAGAT * 9200 * 9220 * 9240 * 9260 * 9280 * 9300 * 9320 * 9340 * 9360 HtaiQT3-mt : TGAAGTTACCTCGGGGATAACTAGGTAAGAAGCAAGGAGAGGCCTTATTGATTTGCTTGGTTGCCATCTCGATGTTGACTTAGGATGTAGCGGAAGGGTGTAGCAGTTTTTCTGGTTGGTCTGTTCGACCTTAGAATCCTTCATGAGTTGAGTTAAGACCGGCGAGAGCCAGGTCGGTTC trnC(Cys)(64) : : 8460 : 8640 : 8820 : 9000 : 9180 : 9360 rrnS(748bp) * 9380 * 9400 * 9420 * 9440 * 9460 * 9480 * 9500 * 9520 * 9540 TTATCTATCTTTGTTGGAGGTTAGTACGAAAGGATTATCTCTAATTAATGTTTGGGTGTGATGCTAGTAAGGCAGGGTGCTCTGCAAAGGCACTTTAGATATTTTTATCCATACCTTATTTATTTAGCTTTGGTTATGGAAGTTTCTCGTGAGCGGTCTACATAGCTAAGTCTTTTGAGG * 9560 * 9580 * 9600 * 9620 * 9640 * 9660 * 9680 * 9700 * 9720 AGCGATGGGGGTGTCGTGTCTGGTGCTAGTAGCGAGTTTTTGATGTTAGGTGGAAGCCTGATCAAGGGTTTATGAGATGTAAGTGGTGAATCCTCAGTGCCAGCATCCGCGGTTATTCTGTTTAGCTTGTACTTCTCTTAAGTTGAAATTCTTTATAAGTGAGTTAGGGTAGGGGGTAGA * 9740 * 9760 * 9780 * 9800 * 9820 * 9840 * 9860 * 9880 * 9900 ATCTTTATCTGGTGGAGTTGTGCTTATAGTGGGTATAAGGGCTTTTTTGAAAAAAGGATTAGAGACCCTTGTATTGAGTAGAGTTATTTTGTGACTCTTTAGTTTAAACTAAAAAGGCTTGGCAGCTGACGAGATTCGTCCGGGGGAACGTGTGTCATAAGAGATGGTCCGCTTAATGGT * 9920 * 9940 * 9960 * 9980 * 10000 * 10020 * 10040 * 10060 * 10080 TTACCAAGATTAGGGGTTAGTGTATATCCGGTTCAATATTCGTGGTTGGTGCCGCAGAGTGAATATAGGGGGGGTTATTAAAGCCAGGTCGATGTGCTGCTAATGACTTGGGGGATTATGCGTTACTGTTTAAAAGAGCCTTCTTCTAGAATGGAGGGGGGTAGTCAGGACTCGGAAGTA : 9540 : 9720 : 9900 : 10080 cox2(624) * 10100 * 10120 * 10140 * 10160 * 10180 * 10200 * 10220 * 10240 * 10260 HtaiQT3-mt : GGTAGGTAATGTTTAGTCTTATCGAAGGGGGATTTAGTTGGGGTACACACCGCCCGTCACCCAAAGCGTTAGCTTTGGTAAGTCGTAACATGGTAATGCTGGGGGAACCGGGCGTTAGTTGGTGGATGGTGTATTTTATCCACTTGATGGCTTCTGTTTTAGTCGGTAAAGAGATTTATG M A S V L V G N E I Y * 10280 * 10300 * 10320 * 10340 * 10360 * 10380 * 10400 * 10420 * 10440 HtaiQT3-mt : AGGGACTTTTGTGGTATCTTGTGTTATTGGTGGCTTTTATTCCAGCTTGGGTATTTTTAGTTTTAGGTTGACAGATTACTGGTAAGTATAATCTTAGAATCGCTGCGAATGAGAAAAATGTCATAGAGTGGGCTTGGACGCTTGTACCTACTTTTATGGTTTTTTACCTGTGTTTTTTGA E G L L W Y L V L L V A F I P A W V F L V L G W Q I T G K Y N L S I A A N E N N V I E W A W T L V P T F M V F Y L C F L * 10460 * 10480 * 10500 * 10520 * 10540 * 10560 * 10580 * 10600 * 10620 HtaiQT3-mt : ATTTGGGTTGTGTAGGGGAGGATATCCCGGTTCGGAGGGAAGGGTTAGTGAAGGTGACGGGTCGACAGTGACTTTGGAGTTATGAAATAAGAGGGGAGGAGAGGGGGTATGATTCAATAATGACGGATTTTGTCGATTCGGTGGATAAGCCTCTGCGGCTGGTTCTTAAAAATTATTATC N L G C V G E D I P V R S E G L V K V T G R Q W L W S Y E I S G E E S G Y D S I M T D F V D S V D K P L R L V L N N Y Y * 10640 * 10660 * 10680 * 10700 * 10720 * 10740 * 10760 * 10780 * 10800 HtaiQT3-mt : ACCTCGTGGTAACTTCGTCGGATGTTATACATAGCTTTGCTCTACCGGAGTTGAATTTGAAGATGGATGCGATACCAGGGCGTTTAAATCAGGGGATAATGTATGCTGACCATTTGGGGTGTTTTGTGGGTTATTGTAGGGAGTTATGTGGTGCAGGACATGCGTATATGCCTATAGTCT H L V V T S S D V I H S F A L P E L N L K M D A I P G R L N Q G I M Y A D H L G C F V G Y C S E L C G A G H A Y M P I V : 10260 : 10440 : 10620 : 10800 nad6(489) (14 bp overlapping with 3’ end of cox2) * 10820 * 10840 * 10860 * 10880 * 10900 * 10920 * 10940 * 10960 * 10980 HtaiQT3-mt : TGGAGGTGATTGCCCCTCGAAGGGGTATTGAGATGTATGATAGCTGCTAGGTTAGTTTTAAGGCTGTATTTTACTACGCTACTGTCTTTTTCTTTTATTACTCATCCGGTAGGGTATTGTTACTTATTACTATTAAGGGCTTTTAGGGTTATTGGTTATGTGTATTTGGTTTTGGGGTTT : 10980 L E V I A P R S G I E M Y D S C M I A A S L V L S L Y F T T L L S F S F I T H P V G Y C Y L L L L S A F S V I G Y V Y L V L G F * 11000 * 11020 * 11040 * 11060 * 11080 * 11100 * 11120 * 11140 * 11160 HtaiQT3-mt : AGATGATACTTGGTTTTGTTCTGTTTGGTTTATGTGGGAGGGGTTTATGTACTCTTTATTTTTGTTTCTATGCATAATCCAAATCCTTCGCCTATGCTGGGTGGGAGTTATGGGTTGGTATTGTTTGTTTATTTTTTTTTGGCTATAAATTTTGTTGGCTATTTTACAGGCTATGAGGGT : 11160 S W Y L V L F C L V Y V G G V Y V L F I F V S M H N P N P S P M L G G S Y G L V L F V Y F F L A I N F V G Y F T G Y E G trnY(Tyr)(63) * 11180 * 11200 * 11220 * 11240 * 11260 * 11280 * 11300 * 11320 * 11340 HtaiQT3-mt : TCTCTGGTGGAGTGTAGTCATTATCTCTGTAGGTATTTTGAGGGGATAACGTACTTGTTTTTTTGCACGATGTTGATGCTGGGTTTTGTGATAGTAAGAATAGTAGGGGGGGAGAAGTGTTCCTTTTATCGATAGAACTAACTTAGTATAAGGAGTACATCGGTTTGTAGGTCCGAGGGT : 11340 S L V E C S H Y L C S Y F E G I T Y L F F C T M L M L G F V I V S I V G G E K C S F Y R * trnL1(Leu1-CUN)(66) trnS2(Ser2-UCN)(64)(3 bp overlapping with trnL1) trnL2(Leu-UUR)(68) * 11360 * 11380 * 11400 * 11420 * 11440 * 11460 * 11480 * 11500 * 11520 HtaiQT3-mt : GATGATTGTTCAGTTGGGGTTAGAGGTGCCAGAGTTTTATGGGGCTGGTTTAGGTCCAGTTAACGATATAAATTATCCCTTTGCGGGCAAGGGTTTGGCCGTTGATGATTTGAAGTCATTAGTTTTTCTTTGTTAGGAATGCTCGCTTAGCTTGGACATGGATGCCAGAGGCGTTAATGG : 11520 trnR(Arg)(64) nad5(1587) * 11540 * 11560 * 11580 * 11600 * 11620 * 11640 * 11660 * 11680 * 11700 HtaiQT3-mt : GTTGGTTTTAAGCATCAAATACGGGTGTTATTCCCTCCGTGTATTGGTCCCGTGTTTGATAACCATTGTGTGGGGTTGCGTTTCGGCCGCAATGAAGGGTTAGATGAACTCTATCAAAAGGTGTTAAGGGCTGTTGTGGTGCTTTTTGTTGCGGTAATGCTTTTCTTGTTATCTATTCTT V L S A V V V L F V A V M L F L L S I L * 11720 * 11740 * 11760 * 11780 * 11800 * 11820 * 11840 * 11860 * 11880 HtaiQT3-mt : GGTTATTGGGGGTGAGGGTGGTCTTTTTCGTGGTTAGGTTTTGTTTTTCGTGACTATGATTTTGTTTTTGTGGTAAGGGATGTGAGTTTGGTTTGTGAAGTAATGCTATTGTTCTGTGGTTCAGTGGCATTATTTTACTGTTTTCATTATTTTGGACAAGGCGCGGATGCTTTTTTTCTT G Y W G W G W S F S W L G F V F R D Y D F V F V V S D V S L V C E V M L L F C G S V A L F Y C F H Y F G Q G A D A F F L * 11900 * 11920 * 11940 * 11960 * 11980 * 12000 * 12020 * 12040 * 12060 HtaiQT3-mt : TATCCATTGATGGCCTGGTTCTTAGGGGTCATGTCTATTCTGGTAATGAGAGGTAGGGTGCTCTTGTCTCTCTTGATGTGGGAATACTTAGGCTTGGTGAGGTTTTTTTTAATCTTATTTTATTCTAACATGGCAAGTCTTCGGGCTTCGTTAATAACTCTTTTTGTCTCACGTTTTGGG Y P L M A W F L G V M S I L V M S G S V L L S L L M W E Y L G L V S F F L I L F Y S N M A S L R A S L I T L F V S R F G * 12080 * 12100 * 12120 * 12140 * 12160 * 12180 * 12200 * 12220 * 12240 HtaiQT3-mt : GATGCCGCCTTATTTATGGTGGTTATGTGGGTTAGATGGTGGTTTGGGGCTTCGTCGATTTTATTGGGATTGCTGCTATTTTTTATTGTTTTAACTAAGAGGGCTTGTTACCCGTTTATTTCGTGGTTACTGGAAGCTATGCGAGCTCCTACGCCAGTAAGTTCACTTGTGCATTCTTCT D A A L F M V V M W V S W W F G A S S I L L G L L L F F I V L T K S A C Y P F I S W L L E A M R A P T P V S S L V H S S * 12260 * 12280 * 12300 * 12320 * 12340 * 12360 * 12380 * 12400 * 12420 HtaiQT3-mt : ACTTTGGTGGCCGCGGGAGTTTGATATCTTTTTTGTTATGGGGATATGTTTAGGTACGGAATGTTAAGGGTATTGTTTATGTTGAGGCTAGTTACTGTTTTAGTAAGGGGGTTTTGTGCTTTGGTATTTAAAGATTTGAAGAAGTTGGTGGCCCTTTCTACTTGTAAAAAAGTTTCTTGA T L V A A G V W Y L F C Y G D M F S Y G M L S V L F M L S L V T V L V S G F C A L V F N D L K K L V A L S T C N N V S W * 12440 * 12460 * 12480 * 12500 * 12520 * 12540 * 12560 * 12580 * 12600 HtaiQT3-mt : TGTATACTGTTTTATGTTTGTGGAAGTCTGGATTTAGCTTTATTACAGTTGATAACACATGGGGTCTGTAAGTGTTACCTCTTTATGTCTATTGGGGACCTGATGGGCTTATCGGGGGGTAGGCAGAGTTCTGTGGGGGTTTATTTGTCCCGTTATGTGGGTCGGTTTTCAGTGTATTTA C I L F Y V C G S L D L A L L Q L I T H G V C K C Y L F M S I G D L M G L S G G S Q S S V G V Y L S R Y V G R F S V Y L * 12620 * 12640 * 12660 * 12680 * 12700 * 12720 * 12740 * 12760 * 12780 HtaiQT3-mt : CAAAGCATTTTGGTGATATCCTTGAGAGGCCTCCCCTTCTTAGGGGTGTTTTTTAGGAAGCATATGTTGGTTGGCTCGGTTTGTTATGTTTATGGGTGAGGGTATTTTGGCTTAATGTTTTTGGGATTTTTTCTTTCTTATGCTTACTGTGTGCGGTTGGTATTGTTGCTTGTTAAGGGC Q S I L V I S L S G L P F L G V F F S K H M L V G S V C Y V Y G W G Y F G L M F L G F F L S Y A Y C V R L V L L L V K G * 12800 * 12820 * 12840 * 12860 * 12880 * 12900 * 12920 * 12940 * 12960 HtaiQT3-mt : CTGGGAGGATTAAGGAGTGGTTATTCGAGTTCCTTTATTATTATTTCTGGTCTGAGCGTAGCGGGGACCTTGTTAAAATCGTGTGGAATGGGTGTGTTAGAAGAGAGTGCGGGATTACTTGTTAGTTATAGCTTTTTTGTAGGAATGATTCAGGTCTTGGGTTGTTTGGGAGGCTTCCTT L G G L S S G Y S S S F I I I S G L S V A G T L L N S C G M G V L E E S A G L L V S Y S F F V G M I Q V L G C L G G F L * 12980 * 13000 * 13020 * 13040 * 13060 * 13080 * 13100 * 13120 * 13140 HtaiQT3-mt : TTTTTTGTCGCCGGTGGGGAGGGGGTCCTTTTTGGGCCTTCTACTTTGAGTTATTCTGATAGGGTAGTTAGGGGGGTGTATGATTTTTATCTTCGTCTCAGTGCTCCTGCTGTTGTGTCTTTTTATCGTTGGGAGTATTATGTTTTTTCTTTGTGGCCACGGTTGACATCTAGTTTTCTC F F V A G G E G V L F G P S T L S Y S D S V V S G V Y D F Y L R L S A P A V V S F Y R W E Y Y V F S L W P R L T S S F L : 11700 : 11880 : 12060 : 12240 : 12420 : 12600 : 12780 : 12960 : 13140 trnE(Glu)(73) * 13160 * 13180 * 13200 * 13220 * 13240 * 13260 * 13280 * 13300 * 13320 HtaiQT3-mt : CTTTTTCGGCTTTCTGCCTTTTCTTTTAAATCCTTGGTTTTGTTTTTGTTGTGTTTTTTATTCTTATACTTTTTAGTCGGTGTTTAAATTACGTTTGAGTTGATTGGGTGTATATGCATTCTAAATTTTCGTTTTAGAGGAGGCCTTTTATTGGGGAGGGGCTAATCAATAATTTTAAAT : 13320 L F R L S A F S F N S L V L F L L C F L F L Y F L V G V * Long Int seq (246)(underlined) * 13340 * 13360 * 13380 * 13400 * 13420 * 13440 * 13460 * 13480 * 13500 HtaiQT3-mt : TTTTAGTTTGATTTTTTTTAATTCTGTCTGAAAGATGCTTGTTTTTTTTATGGGGTGGTTGCCCTAGTTTATCTCTCTGTTGTGAAAACTGTAAGGGGGGGGTATTTTATGTTTTTAGACGTTGTGTTTTATTAGGGAGGTTTTGTGTGTAGAGGGTATAAATTTTATTTTGATAGATTT : 13500 TRU1 (182) * 13520 * 13540 * 13560 * 13580 * 13600 * 13620 * 13640 * 13660 * 13680 HtaiQT3-mt : ATTGTGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAGAAAGGAAATAGGGAGGGGTAATATCTATATTATTATACCTTTAGTTCTAAAAAAAGAAAAGGGAATAAAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGAAAGGGTGATTGTCTCGATGCACTGGGGTTA : 13680 TRU2 (182) * 13700 * 13720 * 13740 * 13760 * 13780 * 13800 * 13820 * 13840 * 13860 HtaiQT3-mt : GTTGTTTGATTGGAAGAGATAAATAGAAAAAAGGAATAGGGAGGGGGGGGACCCCCCCCTAATATCTATTTTTTTATACCTTTAGTTCCAAAAGAAAAAGAGGGATAAGAATATTGCAAAGGGTAAGAAAAGGTTTGTTCCCGGGCACCGAAAGGGAGATTGTCTCGATACACTGGGGTT : 13860 Long Int seq (487) (underlined) * 13880 * 13900 * 13920 * 13940 * 13960 * 13980 * 14000 * 14020 * 14040 HtaiQT3-mt : TGTTTTTGATTGGCAGAGATTAATAGAAAAAAGGAATTGGGAGGGGGGGGGACCCCCCCCCTAATATCTATATTATCATACCATTAGTTCTAAGAGAAAGAAGGAATAGAAATTGGCTAGGATAGATAGGTTTGATGCTTGTGCGCAGAGGGGAATGTCTCAACCACTGGGTTATTGTTG : 14040 * 14060 * 14080 * 14100 * 14120 * 14140 * 14160 * 14180 * 14200 * 14220 HtaiQT3-mt : TCGCAGATATAGAGAAGGATTGGAGGGGGCACCCCCGAATCATATTACACCTTGGTCAATAAAAAGGAATAAAAATTGCAGGAAGAATCGGACCTACGCTAATGTGGATTCCGACCTGTTATGTTCTTAAGCCATGAGAGGACCTTGTGGGTGACCCCTTTTTTCTTTTTAGCGGTGTTC : 14220 * 14240 * 14260 * 14280 * 14300 * 14320 * 14340 * 14360 * 14380 * 14400 HtaiQT3-mt : TAAGAGATGAGGGATGACACTCGCAGGATGGTATGTTTGAGCCTCTCAGTGGAAGGGGGATTCCCCCACCACCCTGTTTAGTTTTTGATTGGAGAGACTATTTAGAAGAAAGGCAGTTGTGAGGGGGGGACCCCCCCCCTTTTTTTTTTTTTTTTCTTGCCCTTATTTTTAAAAGGAAAG : 14400 TRU3 (183) * 14420 * 14440 * 14460 * 14480 * 14500 * 14520 * 14540 * 14560 * 14580 HtaiQT3-mt : AGGGGGGGTAGAATATCTGGCAAGAGATTGGATAGGTTTGAAGCCTCTGCCGCGGAAAGGGGGATTTCCCGATGCACTGTGGTTAGTTGTTTGATTGGGAGAGGTTTATTGGAGAAAACGATTTTGGGAGGGGGGGGGACCCCCCCCCTTATATCTTTTTTTTCATACCCCTATTTTTAA : 14580 TRU4 (183) * 14600 * 14620 * 14640 * 14660 * 14680 * 14700 * 14720 * 14740 * 14760 HtaiQT3-mt : AAGAAGAAGAGGGGATAGAATATTGGCAAAGGATTGGATAGGTTTGAAGCCTGTGCAGCGGAAGGGTGATTTTCTCGATGCACTGTGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAGAAAGGAAATTGGGAGGGGGGGGACCCCCCCCTAATATCTATATTATCATACCATTAGTTC : 14760 TRU5 (183) * 14780 * 14800 * 14820 * 14840 * 14860 * 14880 * 14900 * 14920 * 14940 HtaiQT3-mt : TAAAAGAAGAAGAGGGAATAGAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGGAAGGGTGATTGTCTCGATGCACTGTGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAGAAAGGAAATAGGGAGGGGGGGGACCCCCCCCTAATATCTATATTATCATACCATTAG : 14940 Unique Seq (underlined) trnG(68) * 14960 * 14980 * 15000 * 15020 * 15040 * 15060 * 15080 * 15100 * 15120 HtaiQT3-mt : TTCTAAAAGAAGAAGAGGGAATAGAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGGAGGTAGTATAGGTTTGAGTACGCTGTCTTTCCAAGTCAGATGTCCCGTTGATCGGGTCTCTGCACCTTACTCTTAGTTTACTCTGTTTGAGGCGGGTCTGAATGTGGGGGATG : 15120 nh nh i n gi i g n ùng gi o g n củ mtDNA hoàn ch nh ( oài Haplorchis taichui củ (Htai-LA/GenBank: MG972809) p) cox3(645) * 20 * 40 * 60 * 80 * 100 * 120 * 140 * 160 * 180 Htai-LA(15 : ATGAGGTGGTTACCTGTTTATAATTCTTGAGTGGTTATGGTGGGTATAGTAAGTTTGTTTTTGTGAAAGCTTTGGGGCGTTTTTTTCTTTTTGGGTGCGTTGATTTTTTCGGCTTATTTTCTTGTATGAGAGGTTTTTTTTCCACATTTAAGGCGGTATGCTATGGCTTTTTGACTATTT : 180 * 200 * 220 * 240 * 260 * 280 * 300 * 320 * 340 * 360 Htai-LA(15 : ATAGTTAGTGAGGTGGCTATTTTTGGGAGGTTGCTTTTAACTGTTCTTTGAAAAGATGACGGTGGGGAGGGGGCCTTGTCGGATAGCTTGGAGTTGCCTTTTTTAGGGTGTTTTCTTTTGTTGACTTCCTCTTTGACGGCCACGGTGTATCATCATTGTTTTGGCTTGCCTTATAGTTGG : 360 * 380 * 400 * 420 * 440 * 460 * 480 * 500 * 520 * 540 Htai-LA(15 : GTTTTTTTACTTATCAGTATCTTTTTAGGGTTTGGGTTTGTGTGTTTGCAGTTGTGGGAGTTTTTTGATTGTGAGTGTGATGTTCTTTATTGTACTTATTTTGGGGCTGCTTTCTGTACGGTTGGGTTGCATTTTAGTCATGTGGTTGCGGGGGTCGGTGCTATGACCGTTTTGTTAGTG : 540 * 560 * 580 * 600 * 620 * 640 *trnH(66) * 680 * 700 * cob(1110) Htai-LA(15 : AGGGGTTGGGATACCAATCATTTTTATGCTAGGGTGGTTGTGTGGTATTGGCATTCTGTTGATTATGTGTGGTTGTGAGTATACCTGTTAATATATTACGTTTAGGGTCTTCAATAGGTTATTTAAAACTGTTGTCTTGTGGAGACAGAGATTCGTTGTGTGTGATTGAGGAAGGTTTAA : 720 * 740 * 760 * 780 * 800 * 820 * 840 * 860 * 880 * 900 Htai-LA(15 : TGGTTTCTGTTGTTCGTGGTAAAGTAGTGGATCTTCCTACTAAAGTTTCTTTGAGGTATTTTTGATGTGGGGGGTTTATGATTAGTGTGTTTTTAGTCATTCAGGTTGTATCTGGTGTAATTCTGTCTTTGCTTTATGTAGGAGATGCGGGGGTGAGGTTCGGGTTGGTGCTGGATTTTG : 900 * 920 * 940 * 960 * 980 * 1000 * 1020 * 1040 * 1060 * 1080 Htai-LA(15 : TTAACGAGAGTTTGTTTTTATGATTTGTCCGTTATTTGCATATATGGGGAGTAACGTTTATTTTCCTTCTGTTCTTGGTTCACATGGGGCGTTCTTTTTATTATTCTAGTTATAGGAAGTTGGGGGTATGAAAAGTTGGGTTCGTTCTTTATATTTTAATGATGGTTGAGGCGTTTTTAG : 1080 * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 * 1220 * 1240 * 1260 Htai-LA(15 : GGTATATTCTTCCGTGGCATCAGATGTCTTATTGAGCGGCCACTGTTTTAACCTCGATACTTAAAAGTATACCAGTTGTAGGCGGTAAGCTTTATGAGTTTATTGTTGGGGGCTTTGGGGTCACTAAAGTGACTCTAGTCCGTGTTTTTTCAGCTCATGTGTGCCTGGCTTTTGTGATAA : 1260 * 1280 * 1300 * 1320 * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 Htai-LA(15 : TTGGGCTGAGTGTGATTCATCTTTTTTATTTGCATAAGGGAGGGTCTAATAATCCTTTATCTGCGCCTGGGGGCTACAGGGACGTTGTCCTGTTTCATTCATATTTCACTAGAAAGGATGGGGCGGTGCTTGTATTATTGTTATTACTCCTGGGAGGAGGGTTGATGATATCGCCAGACG : 1440 * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 * 1580 * 1600 * 1620 Htai-LA(15 : CTGTTCTAGATGTGGAAAGGTATATTGAGGCGGATCCGATGGTTACTCCTGTGTCAATAAAGCCTGAGTGGTATTTTCTTGCTTTTTATGCGATGCTGCGGTCTATAGAGTCGAAGGTTGGGGGCTTGGTGCTGGTTTTGATATTCCTGTTCGTTTTGTGATTACCTACTTTTAATTCCT : 1620 * 1640 * 1660 * 1680 * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 Htai-LA(15 : CTTGTGTTTATAGTGTGTTGCGTCAGTATGTATTTTGGGGCTGTTGTTCTCTTTACTTCATTTTGAGCTATCTTGGTGCTTGTCACCCCGAATATCCTTTTGTTTTTATAAGCAAGTTTTGTTCTCTCTTGATTGTTGTATTATTAATGCTGTTTAAGGGGCTGTGGGCAGTTCCTTATT : 1800 nad4L(264) * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 * 1940 * 1960 * 1980 Htai-LA(15 : CTGGGGGGTTTCCTCGTTTGGTTTTGTAGTATGGTTCGGTTGCTTTTGCTTTTAGGCATTTTTATAATTTTGTGTAGGTTTTTTCTTTCTTTGACGCGCTTGTTGAAATGCCTAATAGTGGTTGAGAATTTTAAAGTGTTGGTGTTATTTGTGAGTTTATTATCCCAGCGGGGTGAGACT : 1980 nad4(1281) * 2000 * 2020 * 2040 * 2060 * 2080 * 2100 * 2120 * 2140 * 2160 Htai-LA(15 : TTTATACTTTTCATTGCCTTGATGGTGATTTTTACTATTGAGGTTATTCTTGGTTTGGTGGTTTTGACCCGGTTGTGGGATTCAAGAGGTTTAGTTGGTATAGTGGGTTGATAGGGGCCTTGTCGGTTAGTGGTTTGATTATTTTGGTTGAGGCCTGTGCAGGGTTGGATAGAATCTGGC : * 2180 * 2200 * 2220 * 2240 * 2260 * 2280 * 2300 * 2320 * 2340 Htai-LA(15 : TTAGTCATTCTGGGGTTACGGCGGGAGTTTTTTGTTTTGATGTTATAAGCTTTTATCTGATTCTGCTTTCAATTTTATTATCCTTGTCTTTAAGTTTTTGGGTTGAGAGTATGAGATATTTTAGACGCTTTATGATTTTCTTGAGAGTGCTAGGGTCGCTTTTATGTTACTGTTGTGTTC : * 2360 * 2380 * 2400 * 2420 * 2440 * 2460 * 2480 * 2500 * 2520 Htai-LA(15 : ATGTCGTTTGGTTTTGAGTTTTTTATGAGATGTCAATAGTTCCGTTGCTTGTGTTGTTAATTGTAGATTCTCCTTATTCGGAGCGTTATGTTGCTTCTTGATATCTTTTGGGGTATGTGGTTTTTACGAGTCTCCCAATGTTGCTGTGTATACTTTATTTGTCTTTACTTGAGGGGGGGT : * 2540 * 2560 * 2580 * 2600 * 2620 * 2640 * 2660 * 2680 * 2700 Htai-LA(15 : TTAATATGGGGTGTTGGGGGTCTTCTGATAGGTGGGTTGTAACCGGGAGGTTAATTGTTTTAGCCTTTCTTTTTATTACAAAGATACCTTTGCCTCCGTTTCATGTGTGGCTTCCGATAGTTCATGCGGAGGCTAGGAGCCCAGTATcTGTCTGTTTGAGGGGGTATATAATGAAGTTGG : * 2720 * 2740 * 2760 * 2780 * 2800 * 2820 * 2840 * 2860 * 2880 Htai-LA(15 : GTTTACTTGGTGTCTGCCGGTTTTGTTTTTTTTTACTTCCCGATTTTATTTTCTCTTTGGCTTATACGGTTATATGCTTTTTATGTTCTGTGCTCTTCTTCTTTTCTGCTAGTCGGGAGTTGGATGGTAAGCGCTGACTTGCTTTTTTAAGTCTTTCACACATTTTAGTGGCTATGGGCT : * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 * 3020 * 3040 * 3060 Htai-LA(15 : GTCTGTCTGTGGGGGGTTGAGTATTGAGGGGCTCAGCTATTTTGTATTGTATGGGTCATGGGCTGTCTGCGGGGGTGATGTTTTTATTTCTTTGGTTGATGTATGATGTGGTGGCTACTCGTAATTTAGCTTTGTTGAAGTCGGGGATTTCGGGAAGTTTGTTGTTTCGTGTGTTGGCTT : * 3080 * 3100 * 3120 * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 Htai-LA(15 : GTTGCTGTTTATGTACTGCTTGTTCTTTGCCCCCCACAGTACAATTTTTTTGTGAAGTTTCGTTAATGACTGATGGGGCCTGGAGGGGGGGTTTCTTTTTACTAGTTTTTTATGCTTATGTCTTTATGGGAGGCTTGATGCCTTTATTTTTAGTAGGAAGGTTATTGAGCCGGCATTATG : * 3260 * 3280 * 3300 * 3320 * 3340 *trnQ(66) * 3380 * 3400 * trnF(64) Htai-LA(15 : ATGTGAGGTTTCATGGGGGTTCAGCAATCGGTGTAAAGGTTTATTCTTTATTCTACTTGATTATGTGGAGGTTTGGCTTATTTCTGGTATGTTAAATTGGGGTTTATAGCTTGGTGTAGTTAGAAGCATGCTATGTTTTGGTTGTAGCGGGGATTGGATGTTAGCTATAGTGTTTCCGCC : 2160 2340 2520 2700 2880 3060 3240 3420 atp6(516) * 3440 * 3460 * 3480trnM(64) * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 Htai-LA(15 : TAGCTTAAATTTAAAGCGTTAGTTTGAAGAGCTGAAGATATTATATTGTGGTGGGAGGCGGGATAAGTTAAGTGAGAAACTCTATGGTTCATGTTCATAAAATGCGTGTAGCTCCTGCCTATGTGTTTTTCCCGTGTGAGAAGAGTTTATAATAATGTGATTAGATATGTGGCGGGGGGA : * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 * 3740 * 3760 * 3780 Htai-LA(15 : TTGGGAGATAGATTTTATCGTTTACTTCTTTTTATGGTGTTGTTAGTTTTTTTGGGGTTACGGCTTCCTTATTTTTATGGGATGGGGGGATTCGGTATTTTTATTTTGATTAGGGTTTTCCCCCTTTTTATGAGTTTGTTTTTTTCTCGTTTGATTGATGGTAGTATGAGTGCGTTTTTT : * 3800 * 3820 * 3840 * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 Htai-LA(15 : TGTGGCTTTGTTCCTCAGGGTACTCCGTTGTGGATAGCTCCGTTTGTTTGTTTGGCTGAAACTCTAAGTTATGTTGTGCGGCCCGTGGTTTTGATGATACGTCCTTTTGTAAATCTGAGTATTGGAGCTTTAGGGGGGGCAGTATTGGGTGCTTTAAGTATGACTAGAGGTTGAGTTTTA : 3600 3780 3960 nad2(870) * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 * 4100 * 4120 * 4140 Htai-LA(15 : TTTTTTTTGGTTCTTTTATTTTTTTATGAGGTCTTTGTCGCGTTGGTTCATTGATTTATCGTGTGCAGTATCCTTTCTTTTTCGGAGGATCACTAGAAGGGGGGGGGGTAATCGGGCTTAGATGCGCGGGCTGGCGTTATCTGGTTTTAGGGTGGTCAGTGTTTTCTTGTTCTCTTTATG * 4160 * 4180 * 4200 * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 Htai-LA(15 : CTTGTTCTTGAGAGATAATCTTTTATTATTCTGGCTGTTTTTGGAACTTGGCGCTTTGAGGTTGGTTCCTGGATTTTTTATAGTTGGTGGGAGGGGCGCCTTGGGCGGTCTTTTTAGCTACATTTTGGTTTCTGCGGTTTCTTCTTCGTTAATAGTAAGGGGACTGTTATACGAGGATGT * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 * 4460 * 4480 * 4500 Htai-LA(15 : TTTTTTTCTGTCTTGTTTGGGATTGATAATGAAGTTTGGGGTTTTCCCCTTTATGGGTTGAGTATATGTTGTTGTTTTGGCTAGCAACTGGTTAGTAATATGATTGCTTTCGACTGCTTTAAAGATGGGTTTTTTCTTTTTTTGTTTTTTTATTAGGAGGGGGTGAGATTCTTTGCTTGT * 4520 * 4540 * 4560 * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 Htai-LA(15 : AATGATGTTGTGTGTGTTTACCTTTTTGGTGTTGAGTCTGTTGTTTTGGGTTTTTACTCCTAGTTGACTGGTTTATTGGTGTCATGTCTTGATTAGATCTAGGGCTTCTTTGATACTAATGGCTTTGGAGGTTTCAGTTGATTTACTTTTATGACTGTTTGTTATTTATTTTGTGTGGTC * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 * 4820 * 4840 * 4860 Htai-LA(15 : TACTTGTGCTATTATGCTTTTACGTAGGCTTAGCTTGATGAGCCTGGGTAATAGCGTGGTGGTTTTTTGGGTCGTGTTTATTTTGGTGGCGTTTCCTTTCTCTGTTTCAATTTTTTATAAGTTGGTTGTTAGTCTTTGTATGTACTCGTGTGGTGTTTTAGCATTGTTGGGGTGGGTTGT * 4880 * 4900 * 4920 * 4940 * 4960trnV(60) * 4980 * 5000 * 5020trnA(63) * 5040 Htai-LA(15 : GTATAAAGTTTCGGAGCAGTTTTTTTTAGTTAAGTATATGGTGAGAGAAGGTATTCCCCGTGCGGAGTGAAGAGGAGCAGGTGTGGTTTAAGGGAGGCGAGATAGTTTATTGAAAATGTCTATTTTACGCGTAGAAGAAGGATTACCTATCGTCAGAACGATATAGTTTAATAGGAGATG : 4140 : 4320 : 4500 : 4680 : 4860 : 5040 : 5220 nad1(906) * 5060 * 5080 trnD(67)* 5100 * 5120 * 5140 * 5160 * 5180 * 5200 * 5220 Htai-LA(15 : GCCGCTCTGCAAGCGGTCGGTGAAGGATTTCTATCGTTAGCCTGTTCTTAGTTTATTGTAATAATAGCTTGTCGTGCTATAGGAGGGTGGCTAGACACTCAGAGCGGGGTGACTTGGTTGTTGGGCGTTTATAATTTTCTTAGGTCGTTTTTGGGGTTTGCGGTGATAATGATTTTTGTA * 5240 * 5260 * 5280 * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 Htai-LA(15 : GCCTTCTTTATTTTGGGGGAGCGCAAGGTTTTGGGTTATATGCAGATTCGTAAGGGTCCGAACAAGGTGGGGCTTGTAGGGCTTTTTCAAAGGTTTGCCGATTTGTTGAAGTTGATAATAAAGTTTAAGGTTAGCCTTTATCAGGTTCGTAGGTGACTCTCTTGAATTGGGGTTTATCTT * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 * 5540 * 5560 * 5580 Htai-LA(15 : TTGTTATTTTTGGCGCTTTGTTATTGTTTTGTTTTTGGGTTGTTTTATGGTGGTGTGAGTTGTGTAAAAGGTTTGCTTTGGGTATTGGTGGTAACGAGTATAACGGGGTATAGTCTTTTAAGCATTGGTTGAGGGTCTTATAAAAAGTATGCTTTGGTTAGTTGTGTCCGTTCTGCTTTT * 5600 * 5620 * 5640 * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 Htai-LA(15 : GGGTCGGTGACTTTTGAGGCGTGTTTTATGTGTATTGTGGTGATTGTGGGTGTAGTCTGTGGGGGTTACTTTGGTTACTCGGTGATGGAGAATGCGTGGTTAGTCTTGTTGGTGAGACCTTTAGGATATGCTTTATGATTAGTGGGGATGTTATGTGAGTGTAAACGAACTCCTCTAGAT * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 * 5900 * 5920 * 5940 Htai-LA(15 : TATGCGGAAGCTGAGAGGGAGCTGGTGAGAGGGTTGAATACGGAGTATTGTAAAGTGCCTTTTACTTGCTTGTTTGCTTGTGAGTATCTGATAATGTTTATTTTTTCTTGGTTGGGGGCTTTATTATTTTGGGGAGGGGGCCTAATTTCGTTTTTTAGGTTTGTTCATGTTTTGTTTTTT * 5960 * 5980 * 6000 * 6020 * 6040 * 6060trnN(66) * 6080 * 6100 * 6120 Htai-LA(15 : ATTTGGGCGCGTGCAACTTTACCTCGAGTGCGGTATGATTATTTTGTGGCTTTTATGTGAAAGTACTCAATCCTTATATTGGTTTTTTCGTTATTTTTAGTGGTGTTAGTTTAGTGGTGTGGGTAGATTAATGGTAAAATCGTTAAGCTGTTAACTTAAAGATGATCGGGTATCCTCAGA *trnP(65) * 6160 * 6180 *trnI(65) * 6220 * 6240 * 6260trnK(65) * 6280 * 6300 Htai-LA(15 : CGAGGCAGTCGTATAGTCTATTTTAGGATGTTAGCTTTGGGGGCTTAAGGACTCTTGAGGAGTGGGCTGATAGGGCTGCTGAGCAGGCCACTGTGATATAGTGGTTGTAGGAGAATTAGATCCTCGTCGGTAGACTGTGGGTAGCTTAAGGTGTAAAGTGTTAGATTCTTACTCTAGTGA : 5400 : 5580 : 5760 : 5940 : 6120 : 6300 * 6320 * 6340 * 6360 * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 Htai-LA(15 : TGTTTTTGAACTCCATAGGATGGGTTATTCTGGCTTGTGTGCTCTTTTATTCTTTTTGATTTTTTTATTGGTCGGTGTATTCCATTTATTTTTATGGGATTCTGGTTTGTGAGGGTTTGTAAGTGGGTTTCGGTCTTGGGTCGGTAGGTTTGAGTGTGGTTTTATCTCTCAGGGGGTGTC : * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 * 6620 * 6640 * 6660 Htai-LA(15 : AGAGAAATATTTTAGGTGAACTTACTTTATACTGCTAGTCTTTTTTGTTGTTTTTGACTTGGAAATTTCGTTACTTCTAAAATTACCTTTACAAGGGCTGCTTTTTAAGAAATTGCCCTATTATGTGTGTTTTGTGTTACTCCTGTGCTTTGGTTTTGGGATTGAGGTGAATAAGGGTTA : 6660 nad3(360) 6480 cox1(1542) * 6680 * 6700trnS1(62)* 6720 * 6740 * 6760 trnW(64)* 6780 * 6800 * 6820 * 6840 Htai-LA(15 : TGTCGAGTGAAGCTACTAGGGGTTAGATGTGTGAGAGCTATGTGAAGCTGTTGCTGCTAACAATGGTAAGAGTGTTTAAAGCGCTCGGCTCTCTTGTAGGGAACAATCTAGGTTAAGTAGAGACTGTCTGTCTTCAAAACAGAGGGTAGCTTGCGCTGTTTGTTGTTAGATGTTGACTTG * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 * 6980 * 7000 * 7020 Htai-LA(15 : ATTATTGACTTTGGATCATAAGCGAATAGGGTTGATATATATGATTGTTGGAGTGTGAGGGGGCTTTTTAGGTCTTTCTTTGAGGTTTTTGATCCGTGTTAACTACTTGGATCCTTACTATAATGTTGTATCCCCAGAGGTTTATAATTATGTAATTACTAGGCATGGTATTGCGATGAT * 7040 * 7060 * 7080 * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 Htai-LA(15 : TTTTTTTTTCTTAATGCCGGTTTTGATTGGGGGGTTTGGAAAATATCTGTTGCCCCTGTTGTTGGGGCTTCCTGATTTGAATTTACCCCGTTTAAATGCCCTTAGAGCTTGGTTAATGATTCCTTCCGCTGTTTGTTTTGGGTTAAGTATGTTTGGGGGGTCTGGGGTTGGCTGGACTTT * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 * 7340 * 7360 * 7380 Htai-LA(15 : TTATCCCCCCCTGTCTTCTAGGGATTATAGGGGTTGGGGTGTTGACTTTCTTATGTTTTCTTTACATTTGGCGGGGTTATCTAGTCTGTTTGGTTCTTTAAACTTTATTTGTACAATAATGAGGGCTATGAGTAATGATACCAGGGAGCGGTACTCTATTGTGGTTTGGGCTTATTTGTT * 7400 * 7420 * 7440 * 7460 * 7480 * 7500 * 7520 * 7540 * 7560 Htai-LA(15 : TACTTCTATTCTTTTGTTGCTTTCGTTACCAGTGCTAGCTGCGGGTATAACGATGCTTTTGTTTGATCGTAAATTTAGCTCTTCTTTTTTTGATCCATTGGGAGGGGGGGACCCTGTCTTGTTCCAGCATATGTTTTGGTTTTTTGGGCACCCCGAAGTTTACGTTCTTATCCTCCCAGG * 7580 * 7600 * 7620 * 7640 * 7660 * 7680 * 7700 * 7720 * 7740 Htai-LA(15 : GTTTGGTATAGTGAGACACATTTGTAGGACGTTAACAAATAAAGATTCCTTGTTTGGTTATGGGGGTTTAGTTCTTGCTATGTTTTCTATAGTCTGTTTGGGGAGTGTTGTTTGAGCTCATCATATGTTTATGGTTGGGTTGGATGTTAAGACGGCTGTTTTTTTTAGTTCTGTGACCAT * 7760 * 7780 * 7800 * 7820 * 7840 * 7860 * 7880 * 7900 * 7920 Htai-LA(15 : GGTTATAGGAGTCCCCACAGGTATAAAGGTTTTTTCTTGGCTGTATATGTTGGCGGGAAGTCGGGGCCGGTTTTGGGATCCGATAATGTGGTGGATATTGGGTTTTATTGTCTTATTTACTATCGGGGGGGTGACCGGGATTGTGTTGTCTTCTTCTATAATGGATACTTTGTTGCATGA * 7940 * 7960 * 7980 * 8000 * 8020 * 8040 * 8060 * 8080 * 8100 Htai-LA(15 : CACTTGATTTGTAATAGCTCACTTTCATTATGTTTTATCGTTAGGTTCTTATAGGTCGGTGGTGATATCTATGATTTGATGATGGCCTGTGATAGTGGGTTATAGGTTGAAAAAGTACTTGTTGATGGCTCATTGGGGGGTGTCAATGGTTGGATTCAATTTGTGTTTTTTTCCTATGCA * 8120 * 8140 * 8160 * 8180 * 8200 * 8220 * 8240 * 8260 * 8280 Htai-LA(15 : TTTTTTAGGGTTTCAGGGTTTACCTCGTCGGGTGTGTGTTTATGAGCCTTGTTTTCAGTGGTTGAAAACTATGGCTAGGTTGGGGGGACTTCTTTCTGTTTTTAGGGGGTTTATGTTAGCTTTCATTTTATGAGAGTCGTTGTCTGTGGGTAATCGGGTGATAGGGTTATGGGGTAGTAA : 6840 : 7020 : 7200 : 7380 : 7560 : 7740 : 7920 : 8100 : 8280 : 8460 rrnL(980) Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : * 8300 * 8320 * 8340 * 8360 *trnT(64) * 8400 * 8420 * 8440 * 8460 AGCCTTGGTTTTAAAAGTAGTAACTTCTCCTGTgCCGCATCATTGTAGTTATATGAGTGAGCCGGTAACGTGGTTTTATCGTTGGTATTAGAGGGTGTATTTTAAGGAAGTAGAATGTAGCTTTTGTAAGGCTAAGGTGCTGGGGTCACCCTCTGGTTTATAGGCTGGTTAGGTGCTTAT * 8480 * 8500 * 8520 * 8540 * 8560 * 8580 * 8600 * 8620 * 8640 TTCTGGGTTTATTTTACCTTTTGCATCATGATTCCTTGAGAGATTCCTTGTATAAGGGGTTCCGAAGTGCAGAGATTTCCTTTTGGTTAGCTTAGTGCTGTAGTTTTGGGCGGGTAGCCTTTGTTAAAGTCATTGGGGGAGGTGAAACATAAATCGTTCTGCTCGATATCTGATTTTTGC * 8660 * 8680 * 8700 * 8720 * 8740 * 8760 * 8780 * 8800 * 8820 TAGGGTTTTCTTGGGATCCTTGTTGTTTGGAAATGATGGGGAGGGTAAGAGTCTTGCTGTGTTATTGATGGATTTAGGTTAGAGGTACCTTTTTGTTGTACTATGTGTTAAGACTGGGTAACTTAGACTGTTGAGAGATACTTTGTTGGGGCTAGCAGAGGTTTAATTTTGTGTCGTGAG * 8840 * 8860 * 8880 * 8900 * 8920 * 8940 * 8960 * 8980 * 9000 TATGAGGGAATAAGTAGTTTTATTAGCTCTCTCTTTTCTCGGGTGCCTCCGGTCTGTTTATTAAAAACATTTCCATTAGTAAGGATTAATGGTAGTACCTGCCCGATGTAATAATGAATGGCCGCAGTATCTTGACTGTGCTAAGGTAGCATAATTAGTTGCCTCGTAATTTGGGGATTG * 9020 * 9040 * 9060 * 9080 * 9100 * 9120 * 9140 * 9160 * 9180 TTTGAATGGTTTGACTTGGGGGTTTATCTAGGTGGGGGCTTTGTTGCGAAATTGGTTGATCAGTGCAGAATCTGATCCTTTTTGATTAGACGGAAAGACCCCGAGAGCTTGCAGTAGGATGTTTTATCTGGGGAGGAGGCAAATGTTTCATTTGTTTTTAGACCCTTTTGGGTAAAAGAT * 9200 * 9220 * 9240 * 9260 * 9280 * 9300 * 9320 * 9340 * 9360 TGAAGTTACCTCGGGGATAACTAGGTAAGAAGCAAGGAGAGGCCTTATTGATTTGCTTGGTTGCCATCTCGATGTTGACTTAGGATGTAGCGGAAGGGTGTAGCAGTTTTTCTGGTTGGTCCGTTCGACCTTAGAATCCTTCATGAGTTGAGTTAAGACCGGCGAGAGCCAGGTCGGTTC : 8640 : 8820 : 9000 : 9180 : 9360 rrnS(748) * 9380 * 9400 * 9420trnC(62) * 9440 * 9460 * 9480 * 9500 * 9520 * 9540 Htai-LA(15 : TTATCTATCTTTGTTGGAGGTTAGTACGAAAGGATTATCTCTAATTAATGTTTGGGTGCGATGCTAGTAAGGCAGGGTGCTCTGCAAAGGCACTTTAGATATTTTTATCCATACCTTATTTATTTAGCTTTGGTTATGGAAGTTTCTCGTGAGCGGTCTACATAGCTAAGTCTTTTGAGG * 9560 * 9580 * 9600 * 9620 * 9640 * 9660 * 9680 * 9700 * 9720 Htai-LA(15 : AGCGATGGGGGTGTCGTGTCTGGTGCTAGTAGTGAGTTTTTGATGTTAGGTGGAAGCCTGATCAAGGGTTTATGAGATGTAAGTGGTGAATCCTCAGTGCCAGCATCCGCGGTTATTCTGTTTAGCTTGTACTTCTCTTAAGTTGAAATTCTTTATAAGTGAGTTAGGGTAGGGGGTAGA * 9740 * 9760 * 9780 * 9800 * 9820 * 9840 * 9860 * 9880 * 9900 Htai-LA(15 : ATCTTTATCTGGTGGAGTTGTGCTTATAGTGGGTATAAGGGCTTTTTTGAAAAAAGGATTAGAGACCCTTGTATTGAGTAGAGTTATTTTGTGACTCTTTAGTTTAAACTAAAAAGGCTTGGCAGCTGACGAGATTCGTCCGGGGGAACGTGTGTCATAAGAGATGGTCCGCTTAATGGT * 9920 * 9940 * 9960 * 9980 * 10000 * 10020 * 10040 * 10060 * 10080 Htai-LA(15 : TTACCAAGATTAGGGGTTAGTGTATATCCGGTTCAATATTCGTGGTTGGTGCCGCAGAGTGAATATAGGGGGTTATTAAAGCCAGGTCGATGTGCTGCTAATGACTTGGGGGATTATGCGTTACTGTTTAAAAGAGCCTTCTCCTAGAATGGAGGGGGGTAGTCAGGACTCGGAAGTAGG : 9540 : 9720 : 9900 : 10080 cox2 Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : * 10100 * 10120 * 10140 * 10160 * 10180 * 10200 * 10220 * 10240 * 10260 TAGGTAATGTTTAGTCTTATCGAAGGGGGATTTAGTTGGGGTACACACCGCCCGTCACCCAAAGCGTTAGCTTTGGTAAGTCGTAACATGGTAATGCTGGGGGAACCGGGCGTTAGTTGGTGGATGGTGTATTTTATCTACTTGATGGCTTCTGTTTTAGTCGGTAAAGAGATTTATGAG * 10280 * 10300 * 10320 * 10340 * 10360 * 10380 * 10400 * 10420 * 10440 GGACTTTTGTGGTATCTTGTGTTATTGGTGGCTTTTATTCCAGCTTGGGTATTTTTAGTTTTAGGTTGACAGATTACTGGTAAGTATAATCTTAGAATCGCTGCGAATGAGAAAAATGTCATAGAGTGGGCTTGGACTCTTGTACCTACTTTTATGGTTTTTTACCTGTGTTTTTTGAAT * 10460 * 10480 * 10500 * 10520 * 10540 * 10560 * 10580 * 10600 * 10620 TTGGGTTGTGTAGGGGAGGATATCCCGGTTCGGAGGGAAGGGGTAGTGAAGGTGACGGGTCGACAGTGACTTTGGAGTTATGAAATAAGAGGGGAGGAGAGGGGGTATGATTCAATAATGACGGATTTTGTCGATTCGGTGGATAAGCCTCTGCGGCTGGTTCTTAAAAATTATTATCAC * 10640 * 10660 * 10680 * 10700 * 10720 * 10740 * 10760 * 10780 * 10800 CTCGTGGTAACTTCGTCGGATGTTATACATAGCTTTGCTCTACCGGAGTTGAATTTGAAGATGGATGCGATACCAGGGCGTTTAAATCAGAGGATAATGTATGCTGACCATTTGGGGTGTTTTGTGGGTTATTGTAGGGAGTTATGTGGTGCAGGACATGCGTATATGCCTATAGTCTTG : 10260 : 10440 : 10620 : 10800 nad6 (14 bp overlapping with 3’ end of cox2) * 10820 * 10840 * 10860 * 10880 * 10900 * 10920 * 10940 * 10960 * 10980 Htai-LA(15 : GAGGTGATTGCCCCTCGAAGGGGTATTGAGATGTATGATAGCTGCTAGGTTAGTTTTAAGGCTGTATTTTACTACGCTACTGTCTTTTTCTTTTATTACTCATCCGGTAGGGTATTGTTACTTATTGCTATTAAGGGCTTTTAGGGTTATTGGTTATGTGTATTTGGTTTTGGGGTTTAG * 11000 * 11020 * 11040 * 11060 * 11080 * 11100 * 11120 * 11140 * 11160 Htai-LA(15 : ATGATACTTGGTTTTGTTCTGTTTGGTTTATGTGGGAGGGGTTTATGTACTCTTTATTTTTGTTTCTATGCATAATCCAAATCCTTCGCCTATGCTGGGTGGGAGTTATGGGTTGGTATTGTTTGTTTATTTTTTTTTGGCTATAAATTTTGTTGGCTATTTTACAGGCTATGAGGGTTC * 11180 * 11200 * 11220 * 11240 * 11260 * 11280 * 11300trnY * 11320 * 11340 Htai-LA(15 : TCTGGTGGAGTGTAGTCATTATCTCTGTAGGTATTTTGAGGGGATAACGTACTTGTTTTTTTGCACGATGTTGATGCTGGGTTTTGTGATAGTAAGAATAGTAGGGGGGGAGAAGTGTTCCTTTTATCGATAGAACTAACTTAGTATAAGGAGTACATCGGTTTGTAGGTCCGAGGGTGA * 11360trnL1 * 11380 * 11400 * 11420 trnS2 * 11440 * 11460 * 11480 * 11500trnL2 * 11520 Htai-LA(15 : TGATTGTTCAGTTGGGGTTAGAGGTGCCAGAGTTTTATGGGGCTGGTTTAGGTCCAGTTAACGATATAAATTATCCCTTTGCGGGCAAGGGTTTGTCCGTTGATGATTTGAAGTCATTAGTTTTTCTTTGTTAGGAATGCTCGCTTAGCTTGGACATGGATGCCAGAGGCGTTAATGGGT : 10980 : 11160 : 11340 : 11520 nad5 Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : Htai-LA(15 : * 11540 * 11560 * 11580trnR * 11600 * 11620 * 11640 * 11660 * 11680 * 11700 TGGTTTTAAGCATCAAATACGGGTGTTATTCCCTCCGTGTATTGGTCCCGTGTTTGATAACCATTGTGTGGGGTTGCGTTTCGGCCGCAATGAAGGGTTAGATGAACTCTATCAAAAGGTGTTAAGGGCTGTTGTGGTGCTTTTTGTTGCGGTAATGCTTTTCTTGTTATCTATTCTTGG * 11720 * 11740 * 11760 * 11780 * 11800 * 11820 * 11840 * 11860 * 11880 TTATTGGGGGTGAGGGTGGTCTTTTTCGTGGTTAGGTTTTGTTTTTCGTGACTATGATTTTGTTTTTGTGGTAAGGGATGTGAGTTTGGTTTGTGAAGTAATGCTATTGTTCTGTGGTTCAGTGGCATTATTTTACTGTTTTCATTATTTTGGACAAGGCGCGGATGCTTTTTTTCTTTA * 11900 * 11920 * 11940 * 11960 * 11980 * 12000 * 12020 * 12040 * 12060 TCCATTGATGGCCTGGTTCTTAGGGGTCATGTCTATTCTGGTAATGAGAGGTAGGGTGCTCTTGTCTCTCTTGATGTGGGAATACTTAGGCTTGGTGAGGTTTTTTTTAATCTTATTTTATTCTAACATGGCAAGTCTTCGGGCTTCGTTAATAACTCTTTTTGTCTCACGTTTTGGGGA * 12080 * 12100 * 12120 * 12140 * 12160 * 12180 * 12200 * 12220 * 12240 TGCCGCCTTATTTATGGTGGTTATGTGGGTTAGATGGTGGTTTGGGGCTTCGTCGATTTTATTGGGATTGCTGcTATTTTTTATTGTTTTAACTAAGAGGGCTTGTTACCCGTTTATTTCGTGGTTACTGGAAGCTATGCGAGCTCCTACGCCAGTAAGTTCTCTTGTGCATTCTTCTAC * 12260 * 12280 * 12300 * 12320 * 12340 * 12360 * 12380 * 12400 * 12420 TTTGGTGGCCGCGGGAGTTTGATATCTTTTTTGTTATGGGGATATGTTTAGGTACGGAATGTTAAGGGTATTGTTTATGTTGAGGCTAGTTACTGTTTTAGTAAGGGGGTTTTGTGCTTTGGTATTTAAAGATTTGAAGAAGTTGGTGGCCCTTTCTACTTGTAAAAAAGTTTCTTGATG * 12440 * 12460 * 12480 * 12500 * 12520 * 12540 * 12560 * 12580 * 12600 TATACTGTTTTATGTTTGTGGAAGTCTGGATTTAGCTTTATTACAGTTGATAACACATGGGGTCTGTAAGTGTTACCTCTTTATGTCTATTGGGGACCTGATGGGCTTATCGGGGGGGAGGCAGAGTTCTGTGGGGGTTTATTTGTCCCGTTATGTGGGTCGGTTTTCAGTGTATTTACA * 12620 * 12640 * 12660 * 12680 * 12700 * 12720 * 12740 * 12760 * 12780 AAGCATTTTGGTGATATCCTTGAGAGGCCTCCCCTTcTTAGGGGTGTTTTTTAGGAAGCATATGTTGGTTGGCTCGGTTTGTTATGTTTATGGGTGAGGGTATTTTGGCTTAATGTTTTTGGGATTTTTTCTTTCTTATGCTTACTGTGTGCGGTTAGTATTGTTGCTTGTTAAGGGCCT * 12800 * 12820 * 12840 * 12860 * 12880 * 12900 * 12920 * 12940 * 12960 GGGAGGATTAAGAAGTGGTTATTCGAGTTCCTTTATTATTATTTCTGGTCTGAGCGTAGCGGGGACCTTGTTAAAATCGTGTGGAATGGGTGTGTTAGAAGAGAGTGCGGGATTACTTGTTAGTTATAGCTTTTTTGTAGGAATGATTCAGGTCTTGGGTTGTTTGGGAGGCTTCCTTTT * 12980 * 13000 * 13020 * 13040 * 13060 * 13080 * 13100 * 13120 * 13140 TTTTGTCGCTGGTGGGGAGGGGGTCCTTTTTGGGCCTTCTACTTTGAGTTATTCTGATAGGGTAGTTAGGGGGGTGTATGATTTTTATCTTCGTCTCAGTGCTCCTGCTGTTGTGTCTTTTTATCGTTGGGAGTATTATGTTTTTTCTTTGTGGCCACGGTTGACATCTCGTTTTCTCCT : 11700 : 11880 : 12060 : 12240 : 12420 : 12600 : 12780 : 12960 : 13140 Long Int seq * 13160 * 13180 * 13200 * 13220 * 13240trnE * 13260 * 13280 * 13300 * 13320 Htai-LA(15 : TTTTCGGCTTTCTGCCTTTTCTTTTAAATCCTTGGTTTTGTTTTTGTTGTGTTTTTTATTCTTATACTTTCTAGTCGGTGTTTAAATTACGTTTGAGTTGATTGGGTGTATATGCATTCTAAATTTTCGTTTTAGAGGAGGCCTTTTATTGGGGAGGGGCTAATCAATAATTTTAAATTT : 13320 (underlined) (259) * 13340 * 13360 * 13380 * 13400 * 13420 * 13440 * 13460 * 13480 * 13500 Htai-LA(15 : TTAGTTTGATTTTTTTTAATTCTGTCTGAAAGATGCTTGTTTTTTTTATGGGGTGGTTGCCCTAGTTTATCTCTCTGTTGTGAAAACTGTAAGGGGGGGGTATTTTATGTTTTTAGACGTTGTGTTTTATTAGGGAGTTTTTGTGTGTAGAGGGTATAAATTTTATTTTGAAAAATTTAT : 13500 TRU1 (182) * 13520 * 13540 * 13560 * 13580 * 13600 * 13620 * 13640 * 13660 * 13680 Htai-LA(15 : TGTGGTTAGTTGTTTGATTGGAAGAGGTAAATAGAAGAAAGGAAATAGGGAGGGGGGGGACCCCCCCCTAATATCTATATTATTATACCTTTAGTTCTAAAAAAAGAAAAGGGAATAAAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGAAAGGGTGATTGTCTCGATG : 13680 TRU2 (182) * 13700 * 13720 * 13740 * 13760 * 13780 * 13800 * 13820 * 13840 * 13860 Htai-LA(15 : CACTGGGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAAAAAGGAATAGGGAGGGGGGGGACCCCCCCCTAATATCTATTTTTTTATACCTTTAGTTCCAAAAGAAAAAGAGGGATAAGAATATTGCAAAGGGTAAGAAAAGGTTTGTTCCCGGGCACCGAAAGGGAGATTGTCTCGAT : 13860 Long Int seq (underlined) * 13880 * 13900 * 13920 * 13940 * 13960 * 13980 * 14000 * 14020 * 14040 Htai-LA(15 : ACACTGGGGTTTGTTTTTGATTGGCAGAGATTAATAGAAAAAAGGAATTGGGAGGGGGGGGGACCCCCCCCCTAATATCTATATTATCATACCATTAGTTCTAAGAGAAAGAAGGAATAGAAATTGGCTAGGATAGATAGGTTTGATGCTTGTGCGCAGAGGGGAATGTCTCAACCACTG : 14040 * 14060 * 14080 * 14100 * 14120 * 14140 * 14160 * 14180 * 14200 * 14220 Htai-LA(15 : GGTTATTGTTGTCGCAGATATAGAGAAGGATTGGAGGGGGCACCCCCGAATCATATTACACCTTGGTCAATAAAAAGGAATAAAAATTGCAGGAAGAATCGGACCTACGCTAATGTGGATTCCGACCTGTTATGTTCTTAAGCCATGAGAGGACCTTGTGGGTGACCCCTTTTTTCTTTT : 14220 * 14240 * 14260 * 14280 * 14300 * 14320 * 14340 * 14360 * 14380 * 14400 Htai-LA(15 : TAGCGGTGTTCTAAGAGATGAGGGATGACACTCGCAGGATGGTATGTTTGAGCCTCTCAGTGGAAGGGGGATTCCCCCACCACCCTGTTTAGTTTTTGATTGGAGAGACTATTTAGAAGAAAGGCAGTTGTGAGGGGGGGACCCCCCCCCTTTTTTTTTTTTTTTTCTTGCCCTTATTTT : 14400 TRU3 (183) * 14420 * 14440 * 14460 * 14480 * 14500 * 14520 * 14540 * 14560 * 14580 Htai-LA(15 : TAAAAGGAAAGAGGGGGGGTAGAATATCTGGCAAGAGATTGGATAGGTTTGAAGCCTCTGCCGCGGAAAGGGGGATTTCCCGATGCACTGTGGTTAGTTGTTTGATTGGGAGAGGTTTATTGGAGAAAACGATTTTGGGAGGGGGGGGGACCCCCCCCCTTATATCTTTTTTTTCATACC : 14580 TRU4 (183) * 14600 * 14620 * 14640 * 14660 * 14680 * 14700 * 14720 * 14740 * 14760 Htai-LA(15 : CCTATTTTTAAAAGAAGAAGAGGGGATAGAATATTGGCAAAGGATTGGATAGGTTTGAAGCCTGTGCAGCGGAAGGGTGATTTTCTCGATGCACTGTGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAGAAAGGAAATTGGGAGGGGGGGGACCCCCCCCTAATATTTATATTATCAT : 14760 TRU5 (183) * 14780 * 14800 * 14820 * 14840 * 14860 * 14880 * 14900 * 14920 * 14940 Htai-LA(15 : ACCATTAGTTTTAAAAGAAGAAGAGGGAATAGAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGGAAGGGTGATTTTCTCGATGCCCTGTGGTTAGTTGTTTGATTGGAAGAGATAAATAGAAGAAAGGAAATAGGGAGGGGGGGGACCCCCCCCTAATATCTATATTAT : 14940 * 14960 * 14980 * 15000 * 15020trnG * 15040 * 15060 * 15080 * 15100 * 15120 Htai-LA(15 : CATACCCTTAGTTCTAAAAGAAGAAGAGGGAATAGAATATTGGCAAGGGATAGGATAGGTTTGATGCCTGTGCAGCGGAGGTAGTATAGGTTTGAGTACGCTGTCTTTCCAAGTCAGATGTCCCGTTGATCGGGTCTGTGCACCTTACTCTTAGTTTACTCTATTTGAGGCGGGTATGAA : 15120 * Htai-LA(15 : TGTGGGGGATG : 15131 nh 3 Trình bày di n giải gen v ng giao gen đ n ị h ribosome, ph n m h a loài Haplorchis taichui Việt Nam (chủng Htai-QT3VN) (7.268 bp) 18S(1992) * 20 * 40 * 60 * 80 * 100 * 120 Htai-QT3- : AACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAGAGATTAAGCCATGCATGTCTAAGTACAAACCTTCAAACGGTGAAACCGCGAATGGCTCATTAAATCAGCTATGGTTCCTT : * 140 * 160 * 180 * 200 * 220 * 240 Htai-QT3- : AGATCGTACACTACTACATGGATAACTGTAGTAATTCTAGAGCTAATACATGCCACTATGCCCTGACCCGCAAGGGAACGGGTGGATTTATTAGAACAGAACCAACCGGTGGTGGCTTCG : * 260 * 280 * 300 * 320 * 340 * 360 Htai-QT3- : GCTGCTGCTGTTGCATTCTGTGATGACTCTGGATAACTTTACTGATCGCAGTCGGCCTTGTGTCGGCGACGGATCTTTCAAATGTCTGCCCTATCAATTTTCGATGGTAGGTGACCTGCC : * 380 * 400 * 420 * 440 * 460 * 480 Htai-QT3- : TACCATGGTGATAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACTTCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGGCACGGGG : * 500 * 520 * 540 * 560 * 580 * 600 Htai-QT3- : AGGTAGTGACGAAAAATACGAATACGGGACTCGTTAGAGGCTCCGTAATTCGAATGAGTACACTTTAAATCCTTTAACGAGGATCAACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT : * 620 * 640 * 660 * 680 * 700 * 720 Htai-QT3- : AACTCCAGCTCCAGAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATCTGGGTCGCATGGCTACATGCCGTTGCTTGTATTCCTGGCCTGGTTAACACCGGGACGGGT : * 740 * 760 * 780 * 800 * 820 * 840 Htai-QT3- : TTACGGGTCGGTGTCGTGGTTGTGCAGCCTTTCTGCCGTGTCTGTTTCGACAGGTGTTGGCATGATTGGTGGGTTCGTCCTACTGGTCTGTTGACATGCTCCCAGGTGCCCTTTACTGGG : * 860 * 880 * 900 * 920 * 940 * 960 Htai-QT3- : TGTCGGGGGCGGACGGCACGTTTACTTTGAACAAATTTGAGTGCTCAAAGCAGGCCAATGTGCCTGAAAAGTCTTGCATGGAATAATGGAATAGGACTTCGGTTCTATTTTGTTGGTTTT : * 980 * 1000 * 1020 * 1040 * 1060 * 1080 Htai-QT3- : CGGATCCGAAGTAATGGTTAAAAGGGACAGACGGGGGCATTTGTATGGCGGTGTTAGAGGTGAAATTCTTGGATCGCCGCCAGACAAACTACAGCGAAAGCATTTGCCAAGGATGTTTTC : * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 Htai-QT3- : ATTGATCTGGAGCGAAAGTCAGAGGTTCGAAGACGATCAGATACCGTCCTAGTTCTGACCATAAACGATGCCAACTGACGATCCGTGGTGGCGCGATTATTGGCCCCGCGGGCAGTCCCC : * 1220 * 1240 * 1260 * 1280 * 1300 * 1320 Htai-QT3- : GGGAAACCTTTAAGTCTTTGGGCTCCGGGGGAAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCGACTCAACACGGG : * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 Htai-QT3- : AAAACTCACCCGGCCCGGACACTGTGAGGATTGACAGATTGATAGCTCTTTCTTGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT : * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 Htai-QT3- : AACGAACGAGACTTTGGCCTGCTAAATAGTACGCCTGTCCTCTGCGCTTGTGCAGGTGGCGGTGATCGTTGCCTCTCTGGGGTGATGGTGCCGTTTGCCGGCGAGTGCGGCGCAGGTGTC : * 1580 * 1600 * 1620 * 1640 * 1660 * 1680 Htai-QT3- : TACTTCTTAGAGGGACAAGCGGCGTGCCAGTCGCACGAAATAGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACGTGCGCTACAATGACGGTTTCAACGAGTTTGGG : * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 Htai-QT3- : ATCCTAGCCCGAAAGGGTTGGGTAAACTGAATCATAACCGTCGTGACTGGGATCGGGGCTTGCAATTATTCCCCGTGAACGAGGAATTCCTGGTAAGTGCAAGTCATAAGCTTGCGCTGA : * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 Htai-QT3- : TTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGCAAGGTCCTCGGATTGGTGCCATTGTAGTGGCTTCGGCTGCTCGACCGGTGCTGAAAAGACGA : 120 240 360 480 600 720 840 960 1080 1200 1320 1440 1560 1680 1800 1920 ITS1(797) * 1940 * 1960 * 1980 * 2000 * 2020 * 2040 Htai-QT3- : CCAAACTTGATCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTACACCTAACCGTTATCATCCCGGTTTTCCCGGGTGGTAAGTTC : * 2060 * 2080 * 2100 * 2120 * 2140 * 2160 Htai-QT3- : GGGCTGTCGTGCTCGTACAATCTGCAGCTTGTCTGCCTAAGGCGGAGCGATGAGTTCCGTCATATGTGCTTGTATTTCAGAGATGGAGCGACGCCAGCTCTGTCAAATCCAGTATGCATT : * 2180 * 2200 * 2220 * 2240 * 2260 * 2280 Htai-QT3- : GCATATTGTTGCCTCCATGGCTGACCTCTAACTCGGTAAAGCGACGCCAGCTCTATCGAGTCTCTTGCCCGTTGTTTCTTCGAAGTTGGGCTTTAATTATGCAGCCTTGTCTGCCTAAGG : * 2300 * 2320 * 2340 * 2360 * 2380 * 2400 Htai-QT3- : CGAAGCGATCAGTTTCGTCATGAGTATTGCACTGGTAGATGGAGCGACGCCAGCTCTATCAAATCTTGTATTCATTGGTTTTAATTTGGCTTTTGGCCTTTGATTACGCAGCTTTGTCTG : * 2420 * 2440 * 2460 * 2480 * 2500 * 2520 Htai-QT3- : TCGCTGGGCTATACCTGGTACACACGTGTACCTACGTACAGTCCTTGTTTTGGCGGGGTGTCTACCTGTCTGATGCTCTTGGTGTGCTCGCTCACAGTAGCCAGTCCACACCGGGAGTGA : * 2540 * 2560 * 2580 * 2600 * 2620 * 2640 Htai-QT3- : CGGGTTGTGCTGCTAATATTGGCAGTGCTAGGCTTAAAGAGTGGGCTCGAATATTGTGAAGGGCTACGGCCCACCCACCGCCCCGTATCACGTTGTTTTAAACATATTTTACACTGTTTA : * 2660 * 2680 * 2700 * 2720 * 2740 * 2760 Htai-QT3- : AGTGAGCAGGCATGCTTGTCTTGCTTGTTCTCACTGCCCCGACATGCACCTGGTGCTTGACACTGGACTGCATGTGCAGTCGCCCGGCGGTGCCTTATCCCGGGTACGACTTGCAATCCC : 2040 2160 2280 2400 2520 2640 2760 5.8S(160) * 2780 * 2800 * 2820 * 2840 * 2860 * 2880 Htai-QT3- : GGTCATCTGGTTTACCATTTGATCGATGAGTACAACTCTGAGCGGTGGATCACTCGGCTCGTGTGTCGATGAAgAgCGCAGCCAACTGTGTGAATTAATGTGAACTGCCCAAAGCTTTGA : 2880 ITS2(444) (3 TRU) * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 Htai-QT3- : ACATCGACATCTTGAACGCACATTGCGGCCATGGGTTTTCCTGTGGCCACGCCTGTCCGAGGGTCGGCTTATAAACTATCACGACGCCCAAATAGTCGTGGCTTGGGTCTTGCCAGCTGG : 3000 TRU1(85) * 3020 * 3040 * 3060 * 3080 * 3100 * 3120 Htai-QT3- : CGTGATTTCCTTGTGCTTTTGCATAGGGTGCCAGATCTATGGCTTTTCCCTAATGTGCCGGACGCAACCATGTCTTGGCTGACGGCCTGGATGAGGAAGTGGCGGCGGAGTCGTGGCTCA : 3120 TRU2(83) TRU3(85) * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 Htai-QT3- : ATGAAAATTGTCCGCGCGCTCCAAAGCTTAACCTCTGTCTGGGCTGACGGCTTGGATGAGGAAGTGGCGGCGGAGTCGTGGCTCAATGAAAATTGTGCGCGCTCCAAAGCTTAACCTCTG : 3240 * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 Htai-QT3- : TCTGGGCTGACGGTTTGGATGAGGAAGCGGCGGCGGAGTCGTGGCTCAATGAAAATTGTCCACGCGCTCCAAAGTTTAACCTCTGTCTGAGCTGACGGCTAAGATGTGGCAATGCATCCG : 3360 28S(3875) * 3380 * 3400 * 3420 * 3440 * 3460 * 3480 Htai-QT3- : ATGCAATTCTTTGTGCACTTGAATGTGCCTTATTCCTGACCTCGGATCAGACGTGAATACCCGCTGAACTTAAGCATATCACTAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGT : * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 Htai-QT3- : AACGGCGAGTGAACAGGGAAAAGCCCAGCACCGAAGCCTGTAGCCGTTTGGTTACTAGGCAATGTGGTGTTCAGGTCGTCCCGCGAAGGTACTGCTCCATCCCAAGTCCAGCAATGAGTA : * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 Htai-QT3- : CGGTAATATTTACATGGCCCAAAGAGGGTGAAAGGCCCGTTGGGGTGGAGGCGCAGTAATGCCAGTGCTTTCCTGGGTAGACCTTGGAGTCGGGTTGTTTGAGAATGCAGCCCAAAGTGG : * 3740 * 3760 * 3780 * 3800 * 3820 * 3840 Htai-QT3- : GTGGTAAACTCCATCCAAAGGCTAAATACTAACACGAGTCCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGAGAGAGTAAACAGTACGTGAAACCGCTCAGGAG : * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 Htai-QT3- : GTAAACGGGTGGAGTTGAACTGTGATCTCTGTGAATTTAGCTGGTGAGCATGGTTTGAGCTTGGTCAAATTGGTTGTTACTCGGGGTCTGCTTAGCTGCAGGTCTTTGCCTATGGTGAAG : * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 Htai-QT3- : ATGCGCGAGGCACTTGTCAGGTGTTGCGCGCCCCATGTAATTCAGGCCAGCTTGCCAGTGCACTTTCGCAGAGTGTTCACCACGACCGGCATCGCTGTCCGGCTGATGTGGTTAAACCGG : * 4100 * 4120 * 4140 * 4160 * 4180 * 4200 Htai-QT3- : TCTCGCATTGTCCTTGTGGCTTTGCTTGGTCGGGATGGCAGGTAGCCCTTGGACTTGCTCGCAGCTTGCTGTGGGTTCAGGTCCTTGGGTGTAATCAGCTGACTACATCGGTTCTGTGCA : * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 Htai-QT3- : GTATGTCGGAGACGGCGGCTTGTGGTGTGTGTGCGTGCGTACCAGTTGACTGGCGTGTCCGGGTTTGGTTATTATGTTGCCTGTGTTTACAGGCCTGGTGATGGCCCGGGTTCGTTCGGT : * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 Htai-QT3- : TGACGGTTGCGTGCGTGTCACTTTCCAGGGCCAATAGTCTGTGGTGTTGCGGTAGACTTTCCACCTGACCCGTCTTGAAACACGGACCAAGGAGAGTAACATGTACGCGAGTCATTGGGC : * 4460 * 4480 * 4500 * 4520 * 4540 * 4560 Htai-QT3- : GTAACGAAACCCAAAGGCGAAGTGAAAGTAAAGGTCCGGCTTGTCTGGACTGAGGTGAGATCCTGTCGTTTCTCACACGTGGTATCACCAAGTATCGAGCGACAGGCGCATCACCGGCCC : * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 Htai-QT3- : GTCCCATGGTGTGGTGACATTTTTTGGTGTTCGTCACCGGGGCGGAGCATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGCGCAGGTTGAAGCCAGAGGAAACTCTGG : 10 3480 3600 3720 3840 3960 4080 4200 4320 4440 4560 4680 * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 Htai-QT3- : TGGAGGACCGCAGCGATTCTGACGTGCAAATCGATCGTCAGACGTGAGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGTG : * 4820 * 4840 * 4860 * 4880 * 4900 * 4920 Htai-QT3- : TTCAGAAATATAAACAGTTTTATCCGGTAAAGCGAATGATTAGAGGCCTTGGGGGCGAAACGTCCTCAACCTATTCTCAAACTTTAAATGGGTGAGAAGCCTGGCTCGCCCAAACGGAGT : * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 Htai-QT3- : CAGGCGTTGAATGTGAATGCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGATAAGGTGCCTAACGCGACGCTCATCAGATACCACAAAAGGTG : * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 Htai-QT3- : TTGGTTGGTCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAATGTGTAACAACTCACCTGCCGAATCAACCAGCCCTGAAAATGGATGGCGCTGGAGCGTCGGACCT : * 5180 * 5200 * 5220 * 5240 * 5260 * 5280 Htai-QT3- : ATACCGAACCGTTACTGCAAGCTGAGTCGATAATGTGTGGTGTGAGGACAAGCCGCACGACTCAGGCACGGAATTGTGGTGACGAGTAGGAGGGTCGCCGTGGTGAGCGGGAAGCTTCGG : * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 Htai-QT3- : GCGTAAGCCTGAGTGGAGCCGCCACGGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGGCCGAGGTGGAGAAGGGTTCCATGGGAACAGCAGCTGGCCATGGG : * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 Htai-QT3- : TCAGGCGGTCCTAAGTGATCCGTTAACTCGGAACAGAGACGGGGTGCCTGACATTGTCAGGTTTGCTGCTGATGTACTGTTGCAAGGCAGCCCCCTGTAGCGAAAGGGAATCGGGTTAAT : * 5540 * 5560 * 5580 * 5600 * 5620 * 5640 Htai-QT3- : ATTCCCGACCCTGCCCACGGAGATTGGTGCATTCTGGTCTTGTACTGGTTTGTACCTAGCGCGGTAACGCAACCGACCTCGGAGACGTCGGCAAGAGTTCTGGGAAGAGTTCTCTTTTCT : * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 Htai-QT3- : TTATTAGGAGTGCATCATCCCCTGGAATCGGGTTGTCCGGAGATAGGGGACTAGCCTCCGTAAAGCACCACATTTCATGTGGTGTCCGGAACGCTCTTGTCGGCCCTTGAAAATCCGAGC : * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 Htai-QT3- : AAGGCAATATAATTTTCGTGGCAGGCCGTACCCATATCCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCAATTGAACAATGTAGGTAAGGGAAGTCGGCAAACTGGATCCGTAACTTC : * 5900 * 5920 * 5940 * 5960 * 5980 * 6000 Htai-QT3- : GGGAAAAGGATTGGCTCTGAGGGCCGAGTCAAATGGGCTGGAGTCAGAAACGTTTCGGTCCAGTTAGAAGCTGGTTTTTCTGTTGCTGCGTTTGCCTTCGGGTGAGTGCGGTTGATGGAA : * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 Htai-QT3- : TTACTGGAGTCTGACCAGGCCGATCGTGGGCGATTTCAGCTGTAGTGCTGCTTGGCTGGCAGCGTGCAGGCAACTGTGCGTGTCGACGGCCTTGCTTCCTTCGTTTGGCGCTCAACGGCT : * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 Htai-QT3- : AGCTCAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGTCCGCTGACCGGTTTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG : * 6260 * 6280 * 6300 * 6320 * 6340 * 6360 Htai-QT3- : AAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCTACTGTCC : * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 Htai-QT3- : CTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAGGAGACATGACGGGTGTAGA : * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 Htai-QT3- : ATAAGTGGGAGATGGGCTTCGGTTCATCGCCAGTGAAATACCACTACCGTCATTGTTTCTTTGCTTATTCAGTAAAACGGGAATGATATTTTGGTTCTAAGCACATGCTGGCATGCGTGG : * 6620 * 6640 * 6660 * 6680 * 6700 * 6720 Htai-QT3- : TTCTGCTGCGTGTGTCGGTATCTTGGTCATGTCAGGTGAGTCGTGACTTCGGTTGCGGCGTTTGAGCCTGGATGTGATGTGCGACCCGTTCTGAAGACAATGTCAGGCGGGGAGTTTGAC : * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 Htai-QT3- : TGGGGCGGTACATCTGTCAAATGGTAACGCAGGTGTCCCAAGGTGAGCTCAGCCAGGACGGAAACCTGGCGTAGAGTAAAAGGGCAAAAGCTTACTTGATTTTGATTTTCAGTACGAATA : * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 Htai-QT3- : CAGACCGTGAAAGCGCGGCCTATCGATCCTTTTGATTTTGATATTCAGAGTTTGAAGCAAGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCG : * 6980 * 7000 * 7020 * 7040 * 7060 * 7080 Htai-QT3- : ACGTCGCTTTTTGATCCTTCGATGTCGGCTCTCCCTAGCGTTGTGACGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGAC : * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 Htai-QT3- : AGGTTAGTTTTACCCTACTAATGAGTACAATGAATCAGTGAAGTACAAACTGGCCGTTGCTATGGTAATCCTGTTTAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTATATGTGCCT : * 7220 * 7240 * 7260 Htai-QT3- : GGCCGATAGGCCAATGGTGCGAAGCTACCATCTGAGGGATTAGGACTGAACGCCTCTAAGTCTGAATC : 7268 // 4800 4920 5040 5160 5280 5400 5520 5640 5760 5880 6000 6120 6240 6360 6480 6600 6720 6840 6960 7080 7200 IGS // nh Trình bày di n giải gen v ng giao gen đ n ị h ribosome, ph n m h a loài Haplorchis pumilio Việt Nam (chủng Hpum-HPU8-VN) (7.416 bp) 18S(1992) * 20 * 40 * 60 * 80 * 100 * 120 HPU8-rTUc : AACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAGAGATTAAGCCATGCATGTCTAAGTACAAACCTTCAAACGGTGAAACCGCGAATGGCTCATTAAATCAGCTATGGTTCCTT : * 140 * 160 * 180 * 200 * 220 * 240 HPU8-rTUc : GGATCGTACACTACTACATGGATAACTGTAGTAATTCTAGAGCTAATACATGCCACTATGCCCTGACCCGCAAGGGAACGGGTGGATTTATTAGAACAGAACCAACCGGTGGCGGCTCCG : * 260 * 280 * 300 * 320 * 340 * 360 HPU8-rTUc : GCTGCTGCTGTTGCATTCTGTGATGACTCTGGATAACTTTACTGATCGCAGTCGGCCTTGAGTCGGCGACGGATCTTTCAAATGTCTGCCCTATCAATTTTCGATGGTAGGTGACCTGCC : * 380 * 400 * 420 * 440 * 460 * 480 HPU8-rTUc : TACCATGGTGATAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACTTCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCCGGCACGGGG : * 500 * 520 * 540 * 560 * 580 * 600 HPU8-rTUc : AGGTAGTGACGAAAAATACGGATACGGGACTCGTTAGAGGCTCCGTAATTCGAATGAGTACACTTTAAATCCTTTAACGAGGATCAACTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT : * 620 * 640 * 660 * 680 * 700 * 720 HPU8-rTUc : AACTCCAGCTCCAGAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATCTGGGTCGCATGGCTACATGCTGTTGCTTGTATTCCTGGCCTGGTTTACACTGGGACGGGT : * 740 * 760 * 780 * 800 * 820 * 840 HPU8-rTUc : TTACGGGTCGGCGTCGTGGTTGTGCAGCCTTTCTGCCGTGTCTGTTTCGACAGGTGTTGGCATGATTGGTGGGTTCGTCCTGCCGGTCTGTTGACATGCTCCCAGGTGCCCTTTACTGGG : * 860 * 880 * 900 * 920 * 940 * 960 HPU8-rTUc : TGTCGGGGGCGGACGGCACGTTTACTTTGAACAAATTTGAGTGCTCAAAGCAGGCCCATGTGCCTGAAAAGTCTTGCATGGAATAATGGAATAGGACTTCGGTTCTATTTTGTTGGTTTT : * 980 * 1000 * 1020 * 1040 * 1060 * 1080 HPU8-rTUc : CGGATCCGAAGTAATGGTTAAAAGGGACAGACGGGGGCATTTGTATGGCGGTGTTAGAGGTGAAATTCTTGGATCGCCGCCAGACAAACTACAGCGAAAGCATTTGCCAAGGATGTTTTC : * 1100 * 1120 * 1140 * 1160 * 1180 * 1200 HPU8-rTUc : ATTGATCTGGAGCGAAAGTCAGAGGTTCGAAGACGATCAGATACCGTCCTAGTTCTGACCATAAACGATGCCAACTGACGATCCGTGGTGGCGCGATTATTGGCCCCGCGGGCAGTCCCC : * 1220 * 1240 * 1260 * 1280 * 1300 * 1320 HPU8-rTUc : GGGAAACCTTTAAGTCTTTGGGCTCCGGGGGAAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTCGACTCAACACGGG : * 1340 * 1360 * 1380 * 1400 * 1420 * 1440 HPU8-rTUc : AAAACTCACCCGGCCCGGACACTGTGAGGATTGACAGATTGATAGCTCTTTCTTGATTCGGTGGTTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT : * 1460 * 1480 * 1500 * 1520 * 1540 * 1560 HPU8-rTUc : AACGAACGAGACTTTGGCCTGCTAAATAGTACGCCTGTCCTCTGTACTCGTGCAGGTAGCGGTGGTCGTTGCCTCTCTGGGGTGATGCTGCCGTTTGCCGGCGGGTGCGGCGCAGGTGTC : * 1580 * 1600 * 1620 * 1640 * 1660 * 1680 HPU8-rTUc : TACTTCTTAGAGGGACAAGCGGCGTGCCAGTCGCACGAAATAGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCCGCACGTGCGCTACAATGACGGTTTCAACGAGTTTGGG : * 1700 * 1720 * 1740 * 1760 * 1780 * 1800 HPU8-rTUc : ATCCTAGCCCGAAAGGGTTGGGTAAACTGAATCATAACCGTCGTGACTGGGATCGGGGCTTGCAATTATTCCCCGTGAACGAGGAATTCCTGGTAAGTGCAAGTCATAAGCTTGCGCTGA : * 1820 * 1840 * 1860 * 1880 * 1900 * 1920 HPU8-rTUc : TTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGAATGGTTTAGCAAGGTCCTCGGATTGGTGCCATTGTAGTTGCTTCGGCAGCTCGACCGGTGCTGAAAAGACGA : 120 240 360 480 600 720 840 960 1080 1200 1320 1440 1560 1680 1800 1920 ITS1(1106) * 1940 * 1960 * 1980 * 2000 * 2020 * 2040 HPU8-rTUc : CCAAACTTGATCATTTAGAGGAAGTAAAAGTCGTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGAATGCCTAACCTATATCATCCTGGTTTATCCAGGTGGTGTGTGA : 2040 TRU1 (136) * 2060 * 2080 * 2100 * 2120 * 2140 * 2160 HPU8-rTUc : GTTCTCATGCTCATACGAAAACAAAAATGTGAACCTTGGAAGGATCATTACAGAATTCTAACCTGGACCCTTCGGGCTTGCTATCATATACCAGTGCTACTGGTCTGCAGCTTGGCTGCC : 2160 TRU2(136) * 2180 * 2200 * 2220 * 2240 * 2260 * 2280 HPU8-rTUc : TACGGCAGAGCGCTTTTCAGTTCTGTCATTGTTCAAACAAAAATGTGAATCCTGGAAGGATCATTACAGAAGTCTAACCTGGACCCTTCGGGCTTGCTATcaTATACCAGTGCTACTGGT : 2280 TRU3(136) * 2300 * 2320 * 2340 * 2360 * 2380 * 2400 HPU8-rTUc : CTGCAGCTTGGCTGCCTACGGCAGAGCGCTTTTCAGTTCTGTCATTGTTCAAACAAAGATGTGAATCCGCGAAGGATCATTACAGAAGCCTAACCTGGACGCTTCGGGCTTGCTATCATA : 2400 TRU4(116) * 2420 * 2440 * 2460 * 2480 * 2500 * 2520 HPU8-rTUc : TACCAGTGCTACTGGTCTGCAGCTTGGCTGCCTATGGCAGAGCGCTTTTCAGTTCTGTCATTGTTCATATACGTTGAATTTATATATATATTTGTGAATGGACCCTTCGGGCTTGCTATC : 2520 TRU5(116) * 2540 * 2560 * 2580 * 2600 * 2620 * 2640 HPU8-rTUc : ATATACCAGTGCTACTGGTCTGCAGCTTGGCTGCCTACGGCAGAGCGCTTTTCAGTTCTGTCATTGTTCATATACGTTGAATTTATATATATATTTgTGAATGGACCCTTCGGGCTTGCT : 2640 11 Int Seq (393) * 2660 * 2680 * 2700 * 2720 * 2740 * 2760 HPU8-rTUc : ATCATATACCAGTGCTACTGGTCTGCAGCTTGGCTGCCTACGGCAGAGCGCTTTTCAGTTCTGTCTTCGGGCTATACCTGGTATAAACCTCGTATATACCTACGTACAGTCCGTGTTTCG : 2760 * 2780 * 2800 * 2820 * 2840 * 2860 * 2880 HPU8-rTUc : GCGGGGTGTCTACCTGTCTGATACTCTTGGTGTGCTCGCTTATACAGCCAGTCCACACTGGGAGGACGGGTTGTGCTGCTGTTATGGGCAGTGCTAGGCTTAAAGAGTGGGCTCGAATAT : 2880 * 2900 * 2920 * 2940 * 2960 * 2980 * 3000 HPU8-rTUc : TGTGAAGGGCTACGGCCCACCCACCGCCCCGTATCATACTGTTTTCAACCCATTTTACACTGTTTAAGTGAGCAGGTATGCTGTTTTGGCTGTCTGTTCTCACTGCCCCGACATGCACCT : 3000 5.8S(160) * 3020 * 3040 * 3060 * 3080 * 3100 * 3120 HPU8-rTUc : GGTGCTTGACACTGGACTGCATGTGCAGTCGCCTGGCGGTGCCTTATCCCGGGTACGACTTGCAATCCCGGTCATCTGGGTTACCATTTGATCGATGAGTACAACTCTGAGCGGTGGATC : 3120 * 3140 * 3160 * 3180 * 3200 * 3220 * 3240 HPU8-rTUc : ACTCGGCTCGTGTGTCGATGAAGAGCGCAGCCAACTGTGTGAATTAATGTGAACTGCCCAAAGCTTTGAACATCGACATCTTGAACGCACATTGCGGCCATGGGTTTTCCTGTGGCCACG : 3240 ITS2(280) * 3260 * 3280 * 3300 * 3320 * 3340 * 3360 HPU8-rTUc : CCTGTCCGAGGGTCGGCTTATAAACTATCACGGCGCCCAAAAAGTCGTGGCTTGGGTCTTGCCAGCTGGCGTGATTTCCTTGTGCTATTGCATGGGGTGCCAGATCAATGGCTTCTCCCT : 3360 * 3380 * 3400 * 3420 * 3440 * 3460 * 3480 HPU8-rTUc : AATGTGCCGAACGCAACCATGTCTGGGTTGAATGCCTGGATGAGGGGGTGGCGGCGGAGTCGTGGCTCAATTTATGTATTTTTTATTCATTGTGCGCGCTCCGCTGAAAACCTTCGTCTG : 3480 28S(3870) * 3500 * 3520 * 3540 * 3560 * 3580 * 3600 HPU8-rTUc : TGGCTGTGATGCTAGGATGTGGCAATGCATTCGATGCAAATAATTGTGCACATATGTGCCATATTTACCTGACCTCGGATCAGACGTGAATACCCGCTGAACTTAAGCATATCACTAAGC : * 3620 * 3640 * 3660 * 3680 * 3700 * 3720 HPU8-rTUc : GGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACAGGGAAAAGCCCAGCACCGAAGCCTGTGGCCGTTTGGTTACTAGGCAATGTGGTGTTCAGGTCATTCCGTGAA : * 3740 * 3760 * 3780 * 3800 * 3820 * 3840 HPU8-rTUc : GGTACTGCTCCATCCCAAGTCCAGCAATGAGTACGGTAATTTTTACATGGCCCATAGAGGGTGAAAGGCCCGTTGGGGTGGAGGCGCAGCAATGCCAGTGCTTTCCTGGATAGACCTTGG : * 3860 * 3880 * 3900 * 3920 * 3940 * 3960 HPU8-rTUc : AGTCGGGTTGTTTGAGAATGCAGCCCAAAGTGGGTGGTAAACTCCATCCAAGGCTAAATACTAACACGAGTCCGATAGCGAACAAGTACCGTGAGGGAAAGTTGAAAAGTACTTTGAAGA : * 3980 * 4000 * 4020 * 4040 * 4060 * 4080 HPU8-rTUc : GAGAGTAAACAGTACGTGAAACCGCTCAGAGGTAAACGGGTGGAGTTGAACTGTGATCTCTGTGAATTTAGCTGGTGAACGTGGTTTGAACTTGGTCAAATTGGTTGTTATTTGGGGTCT : * 4100 * 4120 * 4140 * 4160 * 4180 * 4200 HPU8-rTUc : GCTTAGCTGCAGGTCTCCGCCTATGGTGGAGATGCGCGAGGCACTTATCAGGTGTTGCGCGCCTCAGGTGAATCGGACCAGTTCGCCAGTGCATTTTCACAGAGTGTTCACCACGACCGG : * 4220 * 4240 * 4260 * 4280 * 4300 * 4320 HPU8-rTUc : CATCGCTGTCCGGCTGGTATGGTTAAACCGGTCTTGCATTGTCCTCGTGGCTTTGCTTGGTCGGGATGGCAGGTAGCTCTTGGGCTTGCTCGCAGTTCGCTGTGGGTTCAGGTCCTTGAG : * 4340 * 4360 * 4380 * 4400 * 4420 * 4440 HPU8-rTUc : TGTAAACAGCTGACCGTATCGGTTCTGTGCAGTATGTCGGAGACGGCGGCTTGTGGTGTGTGCGTGCGTGCCAGTTGGCTGGCGAGTCCGGGTTTGGTTATTATGTTGCCTGTGTTGGCA : * 4460 * 4480 * 4500 * 4520 * 4540 * 4560 HPU8-rTUc : GGCCTAATGATAGCTCGGATTCGTTCGGCTGACGGTTGCGTGCGTGTCACTTTCCAGGGCCAATAGTCTGTGGTGTTGCGGTAGACTTTCCACCTGACCCGTCTTGAAACACGGACCAAG : * 4580 * 4600 * 4620 * 4640 * 4660 * 4680 HPU8-rTUc : GAGAGTAACATGTACGCGAGTCATTGGGCGTAACGAAACCCAAAGGCGAAGTGAAAGTAAAGGTCTGGCTTGTCTAGACTGAGGTGAGATCCTGTCGTTTCTCACACGTGGTATCCCCAA : * 4700 * 4720 * 4740 * 4760 * 4780 * 4800 HPU8-rTUc : GTATCGAACGGCAGGCGCATCACCGGCCCGTCCCATGGTGTGGTGACACTTCAGTGTTCGTCATCGGGGCGGAGCATGAGCGTACATGTTGAGACCCGAAAGATGGTGAACTATGCTTGC : * 4820 * 4840 * 4860 * 4880 * 4900 * 4920 HPU8-rTUc : GCAGGTTGAAGCCAGAGGAAACTCTGGTGGAGGACCGCAGCGATTCTGACGTGCAAATCGATCGTCAGACGTGAGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCT : * 4940 * 4960 * 4980 * 5000 * 5020 * 5040 HPU8-rTUc : CCGAAGTTTCCCTCAGGATAGCTGGTGTTCAGAAATATAAACAGTTTTATCCGGTAAAGCGAATGATTAGAGGCCTTGGGGGCGAAACGTCCTCAACCTATTCTCAAACTTTAAATGGGT : * 5060 * 5080 * 5100 * 5120 * 5140 * 5160 HPU8-rTUc : GAGAAGCTTGGCTCGCCCAAACGGAGTCAAGCGTTGAATGTGAATGCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGCTGTGGGATGAACCAAACGTTCGGATAAGGTGCCTAACGC : * 5180 * 5200 * 5220 * 5240 * 5260 * 5280 HPU8-rTUc : GACGCTCATCAGATACCACAAAAGGTGTTGGTTGGTCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAATGTGTAACAACTCACCTGCCGAATCAACCAGCCCTGAA : * 5300 * 5320 * 5340 * 5360 * 5380 * 5400 HPU8-rTUc : AATGGATGGCGCTGGAGCGTCGGACCTATACCGAACCGTTGCTGCAAGCTGAGTCGATAATGTGTGGTGTGAGGGCAAGCCGCACGACTCAGGCACGGAATCGTGGTGACGAGTAGGAGG : * 5420 * 5440 * 5460 * 5480 * 5500 * 5520 HPU8-rTUc : GTCGCCGTGGTGAGCGGGAAGCTTCGGGCGTAAGCCTGAGTGGAGCCGCCACGGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGGCCGAGGTGGAGAAGGGT : * 5540 * 5560 * 5580 * 5600 * 5620 * 5640 HPU8-rTUc : TCCATGGGAACAGTAGCTGGCCATGGGTCAGGCGGTCCTAAGTGATCCGTTAACTCGGAACAGAGACGGGGTGCCTGACAATGTCAGGTTTGCTGCTGATGTACTGTTGCAAGGCAGCCC : * 5660 * 5680 * 5700 * 5720 * 5740 * 5760 HPU8-rTUc : CCTGTAGCGAAAGGGAATCGGGTTAATATTCCCGACCCTGCCCACGGAGATTGGTGCATTCTGGTCTTGTGCTGGTTTGTACCTAGCGCGGTAACGCAACCGACCTCGGAGACGTCGGCA : * 5780 * 5800 * 5820 * 5840 * 5860 * 5880 HPU8-rTUc : AGAGTTCTGGGAAGAGTTCTCTTTTCTTTATTAGGAGCGCATCATCCCCTGGAATCGGGTTGTCCGGAGATAGGGGACTAGCCTCCGTAAAGCACCACATTTCATGTGGTGTCCGGAACA : * 5900 * 5920 * 5940 * 5960 * 5980 * 6000 HPU8-rTUc : CTCTTGTCGGCCCTTGAAAATCCGAGCAAGGCAATATAATTTTCGTGGCAGGCCGTACCCATATCCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCAATTGAACAATGTAGGTAAGGG : * 6020 * 6040 * 6060 * 6080 * 6100 * 6120 HPU8-rTUc : AAGTCGGCAAATTGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCCGAGTCAAATGGGCTGGAGTCAGAAACGTTTCGGTCAAGTTAGAAGCTGGTTTTTCTGTTACTGCGTTC : * 6140 * 6160 * 6180 * 6200 * 6220 * 6240 HPU8-rTUc : GCCTTCAGGTGAGTGCGGTCGATGGGAATTCTGGAGTCTGACCAGGCCGATCGTGGGCGATTTCAGCTGTAGTGCTGCATTGCTGGCAGCGTGCAGGCAACTGTGCGTGTCGACGGCTTT : * 6260 * 6280 * 6300 * 6320 * 6340 * 6360 HPU8-rTUc : GCTTCCTTCGTTTGGCGCTCAACGGCTAACTCAGAACTGGCACGGACCAGGGGAATCCGACTGTCTAATTAAAACAAAGCATTGCGATGTCCACTGATCGGTTTTGACGCAATGTGATTT : * 6380 * 6400 * 6420 * 6440 * 6460 * 6480 HPU8-rTUc : CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATG : * 6500 * 6520 * 6540 * 6560 * 6580 * 6600 HPU8-rTUc : AATGGATTAACGAGATTCCTACTGTCCCTATCTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGAAATTAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGAC : * 6620 * 6640 * 6660 * 6680 * 6700 * 6720 HPU8-rTUc : TTTGTGAGGAGACATGACGGGTGTAGAATAAGTGGGAGATGAGCTCCGGCTTGTCGCCAGTGAAATACCACTACCGTCATTGTTTCTTTGCTTATTCAGTAAAACGGGATACACTATTTT : * 6740 * 6760 * 6780 * 6800 * 6820 * 6840 HPU8-rTUc : GATTCTAAGCACATGCTGGCATGCGTGGTTCTGCTGCGTGTGTCGGTATCTTGGTCATGTCAGGGTAGTCGTGACTTCGGTTGCGGCGTTTGAGCCTGGATGTGATGTGCGACCCGATCT : * 6860 * 6880 * 6900 * 6920 * 6940 * 6960 HPU8-rTUc : GAAGACAATGTCAGGCGGGGAGTTTGACTGGGGCGGTACATCTGTCAAACGGTAACGCAGGTGTCCCAAGGTGAGCTCAGCCAGGACGGAAACCTGGCGTAGAGTAAAAGGGCAAAAGCT : * 6980 * 7000 * 7020 * 7040 * 7060 * 7080 HPU8-rTUc : TACTTGATTTTGATTTTCAGTACGAATACAGACCGTGAAAGCGCGGCCTATCGATCCTTTTGATTTTGATATTCAGAGTTTGAAGCAAGAGGTGTCAGAAAAGTTACCACAGGGATAACT : * 7100 * 7120 * 7140 * 7160 * 7180 * 7200 HPU8-rTUc : GGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTCCCTAGCGTTGTGACGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAAC : * 7220 * 7240 * 7260 * 7280 * 7300 * 7320 HPU8-rTUc : GTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTAATGAGTACAATGAATCAGTGAAGTACAAACTGGCCGTTGCTATGGTAATCCTGTTTAGTACGAGAGGAACCG : * 7340 * 7360 * 7380 * 7400 * HPU8-rTUc : CAGGTTCAGACATTTGGTATATGTGCCTGGCCGATAGGCCAATGGTGCGAAGCTACCATCTGAGGGATTAGGACTGAACGCCTCTAAGTCTGAATC : 7416 12 // IGS // 3600 3720 3840 3960 4080 4200 4320 4440 4560 4680 4800 4920 5040 5160 5280 5400 5520 5640 5760 5880 6000 6120 6240 6360 6480 6600 6720 6840 6960 7080 7200 7320 PL2.1 P ương p áp tá DN ết DNA tổng số từ mẫu ng ên ứu tổng số mẫu riêng biệt c tách chi t sinh phẩm DNeasy Blood and Tisue kit (Qiagen Inc M ) ho c GeneJET Genomic DN Purification Kit (Thermo Scientific Inc , M , US ) theo hư ng dẫn nhà sản u t DN tổng số c tách chi t từ SLRN Haplorchis spp nguyên sán trư ng thành thu nhận v i dung t ch hoảng –100 L/mẫu n and Tissue kit (Qiagen Inc n n p m DNeasy Blood ỹ) : - M i mẫu sán ruột nhỏ ( con) c nghi n µL dung d ch Tissue Lysis buffer (ATL) bổ sung µL proteinase-K ( 56oC gi cho n hi mơ c ph n giải hồn toàn - Ti p tục bổ sung - Thêm mg ml) ủ µL dung d ch L l c µL Ethanol ( sang cột c màng l c ly t m %), trộn uủ u, sau 56oC/30 phút chuyển tồn h n d ch vịng ph t ph t, lo i bỏ ph n d ch ph a dư i cột l c - Thêm µL dung d ch Washing buffer vịng ph t - Ti p tục thêm rửa DN , ly t m 3 ( W ) vào cột l c, ly t m ph t, bỏ d ch ly t m bên dư i cột l c µL dung d ch Washing buffer ( W2) vào cột l c ể vòng ph t ph t, bỏ d ch bên dư i, ly t m l i vòng ph t ph t ể làm hô màng - Chuyển cột sang Eppendorf m i, thêm µL dung d ch Elution buffer (EL), ể nhiệt ộ phòng hoảng 2–3 ph t, sau ph t Bỏ cột, thu d ch bên dư i DN mẫu DN –20oC cho ly t m tổng số, ghi vòng ph t hiệu mẫu bảo quản n hi sử dụng - Kiểm tra hàm lư ng DN tổng số cách o h p phụ quang h c (OD, optical density) máy o quang phổ Spectrophotometer (Thermo Scientific Inc.) DN NanoDrop®ND-1000 UV-Vis tổng số c pha thành nồng ộ ng L, sử dụng 1–2 L cho m i phản ứng PCR có dung tích 50 L PL2.2 P ương p áp t ết ế m ung v m u mtDNA Các mồi chung (g i mồi ty thể chung cho sán d t, universal13 platyhelminth primers) c thi t SLRN c liệt ê ể thu toàn mtDN công bố trư c sán d t y (Le et al., 2; 6; c 9) ho c cải ti n bổ sung thêm ể thực PCR dài (long-PCR L-PCR) v i mục tiêu thu nhận o n mtDN c PL2 ) Một số c p mồi ộ dài cao nh t c thể, 4–6 b ho c dài h n (Bảng c hiệu mtDN H taichui c thi t thu nhận sản phẩm PCR ng n ( ,5–3 b) từ huôn DN huôn sản phẩm LPCR ể tổng số ho c từ c thu nhận primer c sử dụng ể giải trình tự Chu i mồi c thi t chư ng trình Primer3Plus (https://www.bioinformatics.nl/cgi-bin/primer3plus/) n n nm Sử dụng c p mồi: i) URNLF URNSR thu v ng gen rrnL-rrnS (~980 bp); ii) thu TRECOBF/TRECOBR o n gen cob (~650 bp); iii) PN D F JN D R thu o n gen nad1 (~400 bp); iv) JB3F JB4 5R thu o n gen cox (444 bp), giải trình tự Các chu i c sử dụng ể thẩm pháp thi t số gen c mức mồi c hiệu h i iểm cho phản ứng PCR L-PCR Một ộ bảo tồn cao nucleotide tRN Gly nh loài danh nh v 6S (rrnL) hay 12S (rrnS) hay chu i sau gen nad5 c sử dụng thi t mồi l n lư t UNI 6F UNI 6R (trên rrnL), UNI 2F UNI 2R (trên rrnS), GLYF GLYR (trên tRNAGly) cho phản ứng L-PCR qua v ng m h a hông m h a mtDN (Bảng PL2 ) ể thực PCR thu nhận mtDN H taichui, Bảng PL2 mồi chung ( mồi) sản phẩm PCR chu i c hiệu ( mồi) c thi t liệt ê t t sử dụng ể thu ch thư c hác Trên c s trình tự nucleotide thu nhận, ti p tục thi t mồi giải trình tự phư ng pháp ti p lồng vào nhau, hay g i phư ng pháp “l o m ” trực ti p từ sản phẩm ho c sau hi tách dịng Giải trình tự c thực t i c s d ch vụ: Macrogen (Hàn Quốc) t i (https://www.macrogen.com/en/main/); Công ty Nam Khoa (Việt Nam): (http://biotechem.com.vn/) PL2.3 P ương p áp t ết ế m g t n t ơn v m osom ể thực PCR thu nhận rTU H taichui H pumilio Việt Nam, Bảng PL2 liệt ê t t mồi c sử dụng ể thu sản phẩm PCR 14 ch thư c hác Trên c s trình tự nucleotide chu i tục thi t mồi giải trình tự phư ng pháp thu nhận, ti p ti p lồng vào nhau, hay g i phư ng pháp “l o m ” trực ti p từ sản phẩm ho c sau hi tách dịng Giải trình tự c thực t i c s d ch vụ: Macrogen (Hàn Quốc) t i (https://www.macrogen.com/en/main/); Công ty Nam Khoa (Việt Nam): (http://biotechem.com.vn/) Mồi sử dụng ể thu nhận rTU H taichui H pumilio Việt Nam (Bảng PL2 4) c thừa từ nghiên cứu trư c dựa số công bố v rTU y c thi t bổ sung c Ng n hàng gen ho c d liệu ch ng công bố ang c (Le et al., 2017; 2020b) ho c c sưu t m ể t h p sử dụng (Olson et al., 2003; Tkach et al., 2016) PCR LPCR c áp dụng phối h p c p mồi hác lệch ể thu ph n DN (ph n o n) dài ng n hác Toàn m i c bi t cho n v rTU (5 8S-ITS -5 8S-ITS2-28S-IGS ) sán lá, n c ộ dài hoảng từ 7–8 cho loài Ph n m h a (coding region) c t nh từ n 9– b, t y thuộc m i u gen 8S cho nh t u gen 28S, ộ dài thư ng hoảng 6–7 ho c b, dài ng n hác Các gen 8S, 8S, 28S rRN c c ch h ộ dài cố nh Tuy nhiên, v ng ITS- (gi a gen 8S gen 8S) ITS-2 (gi a gen 8S gen 28S) v ng l IGS, nhi u c u tr c l p li n loài chi, số loài c thể c c số lư ng hác nhau, nên tổng ch thư c s hác C số mẫu thu c giải trình tự c tồn ph n m h a, hơng thu c v ng l (IGS) PL2.4 n p np n ứng t nP v mt s np m Phản ứng PCR c thể i u chỉnh thành ph n chu trình nhiệt ph h p ể thu sản phẩm PCR tốt nh t c thể thu nhận ph n o n mtDN rTU Ch ng gi i thiệu phản ứng c hiệu su t tốt nh t c thực Phản ứng c tổng thể t ch µL, gồm: 25 µL PCR mastermi (Thermo Fisher Scientific, MA, USA), µL m i lo i mồi ( pmol µL), µL huôn DN (25–5 ng µL), µL DMSO (dimethyl sulfo ide) µL nư c ion DEPC, c thực máy MJ PTC- (US ) Chu trình nhiệt gồm: 15 chu ỳ 95oC ph t, 35 chu ỳ cuối [94oC/1 phút, 52oC/1 phút; 72oC/2–6 ph t], chu ỳ 72oC/10 phút Phản ứng L-PCR c thực v i en yme Phusion DN (Thermo Fisher Scientific Inc ), sản phẩm thu c c polymerase ộ dài 4–6 b v i ch t lư ng tốt Sản phẩm PCR LPCR thu c c iện di iểm tra th ch agarose % nhuộm ethidium bromide, so sánh ộ dài v i th DN Sản phẩm PCR Lamda (HindIII) n b ng c tinh s ch trực ti p sinh phẩm GeneJET PCR Purification Kit (Thermo Scientific Inc ) V i sản phẩm a b ng, b ng DN ng ch thư c dự t nh c ph n lập từ th ch agarose sinh phẩm GeneJET Gel Extraction Kit (Thermo Scientific Inc.) Các sản phẩm PCR c tinh s ch sinh phẩm GeneJET giải trình tự t i c s d ch vụ PL2.5 P ương p áp tá PCR Purification Kit c gửi i gi i thiệu dòng t u DNA pl sm d tá tổ ợp Sản phẩm PCR ho c c giải trình tự trực ti p, n u c ch t lư ng tốt hàm lư ng ủ theo yêu c u Một số sản phẩm c tách dòng sử dụng vector T cloning thư ng m i pCR2 TOPO (Invitrogen) ho c TOPO XL PCR Cloning Kit (Invitrogen Inc) (n u sản phẩm PCR