Báo cáo khóa học: The role of N-linked glycosylation in the protection of human and bovine lactoferrin against tryptic proteolysis pdf

... The role of N-linked glycosylation in the protection of human and bovine lactoferrin against tryptic proteolysis Harrie A. van Veen, Marlieke E. J. Geerts, Patrick H. C. van Berkel* and Jan ... NuijensPharming, Archimedesweg, Leiden, the Netherlands Lactoferrin (LF) is an iron-binding glycoprotein of the innate host defence system. To elucidate the role of N-linked glycosylation in protection of ... herein further elucidate the role of N-linked glycosylation in the protection of lactofer-rin against tryptic proteolysis. It appeared that glycosylation at Asn281 protects bLF against trypsin....

Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

... the context of DNA binding and chromatin regulation. These homologues includemembers of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated CpGislands [7], which are ... inhi-bitor of growth protein 3 (ING3) PHD, which isanother domain involved in chromatin binding and clearly more divergent (e-value 0.019) from DIDO and the other sequences. As the alignment ... from the other bran-ches, which was indeed predicted. The other maingroup, obtained 97% of the time in 20 740 exploredtrees, contained the DIDO PHD sequences clusteredwith the PHF and the...

Báo cáo khoa học: A role for serglycin proteoglycan in granular retention and processing of mast cell secretory granule components ppt

... Tissue-speciﬁcexpression of mast cell granule serine proteinases and their role in inﬂammation in the lung and gut. Immunol-ogy 105, 375–390.5 Caughey G (2001) New developments in the genetics and activation of ... on serglycin. Together,these ﬁndings indicate that the reduced amounts of neutral proteases in the absence of serglycin is not caused by missorting into the constitutive path-way of secretion, ... immu-nohistochemistry, and were released following exposure to calcium iono-phore, indicating that they were correctly targeted into secretory granuleseven in the absence of serglycin. In the absence of serglycin,...

Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf

... stabilityfor crystallins is essential in maintaining lens transparency[4]. Determining the mechanism of folding and assembly of these proteins is important for understanding how they canform stable ... concentrations. The d2-crystallin in lens was recruited during evolutionfrom argininosuccinate lyase, an enzyme involved in arginine biosynthesis derived from the urea cycle. Thesetwo proteins shared ... conformationalstates in the pathways leading to folding and unfolding areimportant. For d2-crystallin, characterization of the inter-molecular association of the helix bundles and the partialunfolded intermediate...

Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx

... chain is brefeldin A-insensitive and isinhibited by the proteasome inhibitor clasto-lactacystinb-lactone, resulting in the accumulation of ricinA chains. These stabilized ricin A chains are partlydeglycosylated ... pivotal role of the UPR is to maintain ER home-ostasis. Therefore, the presence of mutated proteinsthat are unable to fold into their native conformation in the ER induces the UPR in an effort ... peptide in the 24-kDa a-zein and the 19-kDa a-zein endosperm storage proteins, respect-ively. These mutant proteins are translocated into the lumen of the ER, but remain anchored to the mem-branes...

Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterﬂy, Papilio xuthus docx

... staining of the gel indicates that the ﬂuorescing protein is one of the major components of soluble proteins in the crude extract. The surface of the ﬂuorescing protein carries negative charge in ... cellular retinal-binding protein; CRBP,cellular retinol-binding protein; IRBP, interphotoreceptorretinoid-binding protein; RBP, retinol-binding protein.Note: The nucleotide sequence reported in this ... kind of retinol-binding protein possibly localized in the Papilio retina, and functions in the visual cycle and/ orcolor vision. In this study, we therefore isolated a soluble retinol-binding...

Báo cáo khoa học: Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases ppt

... important role in the productive interaction between the A domain and the PCP domain. The recently reported crystal structure of a termina-tion module from the surfactin NRPS, consisting of the domains ... for the investigation of the highly homologous protein tyrocidine synthetaseI (TycA) in its apo-form. They found that the addition of the substrates slowed down the proteolysis of the protein, ... by decreasing the rate of cleavagebetween the two subdomains of the A domain. Theseﬁndings were later explained with the structural modelthat the smaller subdomain AC of the A domainrotates...

Tài liệu Báo cáo khoa học: A genetic screen identifies mutations in the yeastWAR1 gene, linking transcription factor phosphorylation to weak-acid stress adaptation docx

... were raised in rabbits against a GST-War1p fusion protein containing 294 aminoacids of the C-terminal part of War1p (amino acids 650–944) fused in- frame to the C-terminus of GST. The genefusion ... expression of the C-terminal 294 amino acids of WAR1 fused in- frameto the C-terminus of GST. Escherichia coli strain DH5aharboring pCG1950 was grown on 37 °CtoanA600 of 0.5.Expression of the GST-War1p ... sorbic,benzoic and propionic acid [7] and fail to adapt to suchstress conditions in the absence of Pdr12p. Moreover,recent data indicate the involvement of Pdr12p in the export of by-products of amino-acid...

Tài liệu Báo cáo khoa học: "A HYBRID APPROACH TO REPRESENTATION IN THE JANUS NATURAL LANGUAGE PROCESSOR" pot

... defining the set of items that have ever been the actor in a flight. Their definition is solely within NIKL using the QUA link [14], which is exactly the set of fillers of a slot. While having ... expressions in inten- sional logic. 3 4. Use of the Taxonomy in Janus By domain mode/we mean the set of axioms en- coded in NIKL regarding the constants. The domain model serves several purposes in ... 99.9% of the examples in our expert system and data bass applications have fit well within the constraints of NIKL. Based on our experience and that of others, the axioms and limited inference...

Báo cáo khoa học: "Parsing Free Word Order Languages in the Paninian Framework" pptx

... corresponding to (d, k) in V to the node corresponding to s in U. The original problem of finding a solution parse in the constraint graph now reduces to finding a complete matching in the bipartite ... theta roles, and the neat de- pendence of the karaka vibhakti mapping on TAMs 106 and the transformation rules, in case of Indian lan- guages. The same principle also solves the problem of ... later in Sec. 3.) 2 Constraint Based Parsing The Paninian theory outlined above can be used for building a parser. First stage of the parser takes care of morphology. For each word in the input...

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