Báo cáo khoa học: "The Formal and Processing Models of CLG" docx

... The Formal and Processing Models of CLG Luis DAMAS Nelma MOREIRA University of Porto, Campo Alegre 823 P-4000 Porto luis@nccup.ctt.pt ... a family of grammar formalisms based on complex constraint resolution designed, implemented and tested over the last three years. CLG grammars consist of the description of global and local ... the CLG processing model are the use of constrained terms to represent partial descriptions, the lack of systematic rewriting of constraints to normal form and the lazy evaluation of complex...

Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elaﬁn) do not depend on its protease inhibitory function pptx

... and the CFU counted. The per-centage of surviving CFU was calculated by the formulaN ⁄ Ncontrol· 100, where N and Ncontrolwere the numbers of CFU obtained after 3 h of incubation with and ... (2006)Secretion of serine peptidase by a clinical strain of Candida albicans: inﬂuence of growth conditions and cleavage of human serum proteins and extracellularmatrix components. FEMS Immunol Med ... exacerbations of COPD. Infection 35, 143–149.38 Higuchi R, Krummel B & Saiki RK (1988) A generalmethod of in vitro preparation and speciﬁc mutagenesis of DNA fragments: study of protein and DNA...

Tài liệu Báo cáo khoa học: The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB pdf

... Fragments of HSQC and HMBCspectra of compound 1. Labels illustrateassignment of the amide linkage between theamino group of Asp and the carboxyl group of GalA residue E.Scheme 1. The structures of ... min), dried and analyzed by GC-MS with the standardsprepared fromD-GalN and (R)- and (S)-2-BuOH.The absolute conﬁguration of L-aspartic acid was deter-mined by chiral HPLC of the oligosaccharide ... constants and 13C NMR chemical shifts.Anomeric conﬁgurations were deduced from the J1,2coupling constants and chemical shifts of H-1, C-1 and C-5 signals. The position of C-6 signals of uronic...

Báo cáo khoa học: "Combining data and mathematical models of language change" ppt

... course of the N/V stress shift, and can thus be seen as pos-sible sources of the diachronic dynamics of N/Vpairs.55This type of assumption is necessary for any hypothesisabout the sources of ... N1examples of the N form, of which kt1areσ´σ and (N1-kt1) are ´σσ; N2 and kt2are similarlydeﬁned for V examples. Each example is sampledi.i.d. from a random member of Gt−1. The ... probabilities of producing N and V examplesas σ´σ. αt, βtare the expectation of ˆαt and ˆβtovermembers of Gt: αt= E(ˆαt), βt= E(ˆβt). ˆαt and ˆβtare thus random variables (over...

Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt

... Chimera1C2 encoded residues 1–505 and 653–725 of TAP1 and residues 494–639 of TAP2 and chimera 2C1 encoded residues1–493 and 640–703 of TAP2 and residues 506–652 of TAP1.The exchange was performed ... EcoRI site of pHbApr1neo [22] and sequenced fully in both directions.Chimera 1V2 encoded residues 1–652 of TAP1 and residues640–703 of TAP2 and chimera 2V1 encoded residues 1–639 of TAP2 and residues ... exchange and expression in T2 cells. (A)AminoacidalignmentoftheNBDsofratTAP1a and rat TAP2a.Sequences were retrieved from the GenBank database (GenBank X57523 and X63854) and aligned using the softwareVECTOR...

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... dis-tribution of the energy between the overall tumbling and the internal mobility, and is indicative of thedegree of internal restraint of each15N–1H vectormotion. In Fig. 4, most of the points ... graph of R1, R2 and {1H}–15N NOE val-ues along the sequence of the TTF1 HD at 11.7 T and 286 K.Fig. S2. Plot of J(0.87xN)–J(0) correlation from15Nrelaxation measurements of the ... helix I, Ser27 and Leu34in helix II, and Lys46 and Gln50 in the N-terminalportion of helix III. For an isotropically tumblingglobular molecule, in the absence of internal motions and with relaxation...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... concentration of Rep245 pre-pared in 10 mm sodium phosphate (pH 7.4) was 5 lm. CDspectra were recorded at 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of 1 ... presence of 0.2 mmdNTPs and 0.5 U of Taq DNA polymerase (Promega).To analyze the effect of temperature on primase activity,Rep245 and Rep516 proteins were incubated with 0.25 lg of M13 mp18 ... 2008 FEBS 4391Zn-binding motif and the two disulﬁde bonds respec-tively connecting the a4-helix to the b4 strand and theb9 strand to the b10 strand at the bottom of theZn-stem loop in the pRN1...

Tài liệu Báo cáo khoa học: The diacylglycerol and protein kinase C pathways are not involved in insulin signalling in primary rat hepatocytes doc

... SwitzerlandDiacylglycerol (DAG) and protein kinase C (PKC) isoformshave been implicated in insulin signalling in muscle and fatcells. We evaluated the involvement of DAG and PKC in theaction of ... fraction(except for the nonmobile f-isoform; samples of control and PMA-treated cells were run on each gel for compar-ison), and (d) comparison of bands after incubation of amembrane blot with buffer ... presence or absence of an antigen (PKC isoform) of the respective PKCantibody. The bands on the immunoblots at about80 000 molecular mass, representing PKC isoforms a, d and f, and at 90 000 molecular...

Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... (forward:5¢-ACTACCTCATGAAGATCCTG-3¢ and reverse:5¢-TTGCTGACCACATCTGCTG-3¢) served as a controlfor the quantity and quality of cDNA.Semiquantitative analysis of RT–PCR products. Suspen-sions of Carp head kidney and liver ... isolated and its genomic structure and expression analysis investi-gated. This work will pave the way for further investigation of the biological function of this gene, and the probability of the ... against products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of thecarp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation...

Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

... intenseband of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), conﬁrming its hetero-oligomeric nature(Fig. 1, lane 1).However, in the absence of boiling (Fig. 1A, lanes 2 and 4) ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at37 kDa, suggesting the presence of dimers. All of the bandsstained positively ... B.H., Scheidt, R. & Osvath, S.R. (1986) Models of the cytochromes b6. The eﬀect of axial ligand planeorientation on the EPR and Mo¨ssbauer spectra of low-spin fer-rihemes. J. Am. Chem. Soc....

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