... might rain.’ Michael has just cut It’ssoon a badThe cut.tank You is say to him: □ We’d better stophimself for petrol almost empty (a plaster) The negative is I’d better not (= I...
... structure DTNB has often been used to probe the nucleophilic reactivity of thiolates in MTs DTNB contains an intramolecular disulfide bond and, upon nucleophilic attack from MT, disulfide exchange occurs ... deprotonation at physiological pH, yielding Zn–thiolate complexes This renders thiolates more nucleophilic than thiols However, their binding to Zn(II) generally also inhibits the formation of disulfides ... MTs [5] Third, the deprotonated cysteines bound to the metal ions (i.e thiolates) are good nucleophiles, conferring a high reactivity with radical species (HO·, O2·), NO·) as well as with alkylating...
... Ó FEBS 2002 further 10 Peak fractions were collected and lyophilized Heterogeneous fractions were resolved further on a porous graphitic carbon (PGC)-column (4.6 ´ 100 mm, Hypercarb; Hypersil, ... MS and (B) MALDI-TOF MS of complex S mansoni egg glycosphingolipids (C) MALDI-TOF MS of oligosaccharides released from S mansoni egg glycosphingolipids order to reduce peak heterogeneity and obtain ... novel type of glycosphingolipid J Biol Chem 267, 2251±2257 16 Khoo, K.H., Chatterjee, D., Caul®eld, J.P., Morris, H.R & Dell, A (1997) Structural characterization of glycosphingolipids from the...
... transition states [2–4] The catalytic nucleophile and acid ⁄ base groups are both usually carboxylates, most frequently glutamate, as shown by labelling, crystallographic and mutagenesis studies [5– 7] ... unexpected carbohydrate binding site Previous studies had shown that mutating the catalytic nucleophile E292 to glutamine resulted in an inactive enzyme [24,25] The crystal structure analysis of ... molecule by the catalytic base E192 Protection of the covalent intermediate from the water nucleophile via occlusion of the catalytic base by bound product or incoming acceptor has been proposed...
... helix and Fig Analysis of a predicted long amphipathic a-helix of AR18–35 in AR N/C interaction (A) A helical wheel drawing of AR18–35 predicts a long amphipathic a-helical structure Gray circles ... hormone receptors Genes Dev 13, 3209–3216 31 Uesugi, M & Verdine, G.L (1999) The alpha-helical FXXPhiPhi motif in p53: TAF interaction and discrimination by MDM2 Proc Natl Acad Sci USA 96, 14801–14806 ... N/C interaction The predicted structure indicated that AR16)36 can fold in a remarkably long amphipathic a-helical structure, suggesting an important protein interaction interface [29] AR16)36...
... Africa Venoms were collected by electrical stimulation and lyophilized after dilution in a saline buffer or distilled water The lyophilized venom was dissolved in 100 mM ammonium acetate, pH (Merck, ... 4-mL fractions were collected automatically The fraction containing the toxin was recovered, lyophilized and applied on a PepRPC HR 5/5 C2/C18 reversed-phase FPLC column (Pharmacia, Sweden) equilibrated ... fractionated by FPLC gel filtration, yielding four peaks The labelled fraction (*) was recovered and lyophilized Based on a constructed gel filtration calibration curve, the molecular mass of the material...
... direct style and block the text in paragraphs to help the eye scan it Use meaningful graphics • Use icons or graphics (with a text equivalent) where they facilitate comprehension of the page • Provide...
... and applications / edited by Bernd Jähne, Horst Haussecker, Peter Geissler p cm Includes bibliographical references and indexes Contents: v Sensors and imaging — v Signal processing and pattern ... 17.9 Conclusions 17.10 References 18 Topographical Maps of Microstructures T Scheuermann, G Wiora and M Graf 18.1 Introduction ... and integral equations, complex numbers, Fourier transform, probability, random variables, and graphing Wherever possible, mathematical topics are described intuitively In this respect it is very...
... desensitization of neutrophils to stimulation by any two neutrophil CEACAMs allows the cell to respond to stimulation by the other two neutrophil CEACAMs However, neutrophils desensitized to CEACAM-1 ... signals in neutrophils resulting in activation of CD11/CD18, and an increase in neutrophil adhesion to endothelial cells [37], potential interactions among these molecules in neutrophil activation ... signaling in neutrophils To examine potential interactions among CEACAM members in transmitting signals in neutrophils, the effects of a set of well characterized CD66 mAbs on neutrophil adhesion...
... performed on a clinical tesla MRI unit (Intera, Philips Medical System, Netherlands, B.V.) with using a solenoid cm radiofrequency receive only coil (Philips Research Laboratories, Germany) for rat ... Program of the Clinical Center at the National Institutes of Health We would also like to acknowledge Philips Medical Systems as part of a cooperative research and development agreement for providing...
... infant feeding options for HIV-infected mothers We used descriptive statistics to examine the demographic characteristics of the participants and their experiences with and attitudes towards RCT The ... Lemeshow goodness-of-fit test results that were not significant (p-value > 0.05) Results Socio-demographic characteristics Of the 388 new antenatal attendees enrolled in the study, about two-thirds...
... which have an automor3 phism group acting regularly on both the set of the point classes and the set of the block classes They said such automorphism group a GL-regular automorphism group Especially ... nonisomorphic STD7 [21, 3]’s with this automorphism group Two of these STD7 [21, 3]’s are new and the remaining one is an STD constructed in [14] We also investigate the order of the full automorphism ... isomorphic each other and this STD is isomorphic to D(H6 ) (ii) We also checked that the tensor product of the STD2 [6, 3] and the STD1 [3, 3] yields an STD6 [18, 3], but this STD is isomorphic...
... explain activation of FLSs in RA and it is evident that additional factors, such as genetic polymorphisms, play an important role, because bacterial cell wall components, such as peptidoglycan, have ... manuscript Acknowledgements We would like to thank Patrick Garnero for pro-MMP-3 assay and Claude Philippe for technical assistance with real-time PCR We are grateful to Francis Raul, in whose laboratory...
... Johnson WG, Guy SG, Nye JS, Brown S: Possible association of NTDs with a polyhistidine tract polymorphism in the ZIC2 gene Am J Med Genet 2002, 108:128-131 Wallis DE, Muenke M: Molecular mechanisms...
... neutrophil apoptosis in a dose-dependent fashion [32] Certain cytokines increase the lifespan of the neutrophils by inhibiting their apoptotic cell death in vitro and this prolonged neutrophil ... phenotype of and superoxide anion generation by neutrophils Infect Immun 1990, 58:1647-1652 Berker E, Kantarci A, Hasturk H, Van Dyke TE: Effect of neutrophil apoptosis on monocytic cytokine response ... peripheral bloodneutrophils in vitro Am J Respir Cell Mol Biol 1992, 7:507-513 Gamonal J, Sanz M, O'Connor A, Acevedo A, Suarez I, Sanz A, Martinez B, Silva A: Delayed neutrophil apoptosis in chronic...
... 21:3162-3168 Feigin RD, Cherry JD, editors: Textbook of Pediatric Infectious Diseases 4th edition Philadelphia, PY: W.B Saunders Company; 1998 Palmu AA, Verho J, Jokinen J, Karma P, Kilpi TM: The seven-valent ... was reviewed in order to ensure compliance with the inclusion and exclusion criteria Their demographic data and medical history were recorded at enrolment, and they underwent a physical examination ... only data of the children who completed the follow-up are presented Table summarises the demographic characteristics of the children, and shows that the two groups were comparable, with no significant...