applicable the functional currency of a foreign operation shall be disclosed specifying the net investment therein when this is not the same as the presentation currency of the annual accounts
... Effective date: The contract or agreement should have a date stated asthe contract date or effective date This date isnot necessarily the date whenthe contract was signed but rather the date from ... their home office is located This designation generally benefits the company because it is operating under these laws already, is familiar with them, and has attorneys who are familiar with them ... by annex of fax Any signature to this contract transmitted by fax shallbe deemed as original This contract is made into English originals having thesame value and effected from the signing date...
... FITC was measured in the FL1 channel (510–535 nm bandpass filter) Data were recorded and analyzed with flowmax software from Partec Statistical analysis of ELISA experiments Each experiment was repeated ... parallel) at a rate of 30 lLÆmin)1 for (association phase) The dissociation phase was evaluated during the following 10 The vehicle was always injected in parallel-flow channels The running buffer, NaCl ... Biorad) was also used to dilute the analyte All ofthe assays were performed at 25 °C The sensorgrams (time course ofthe SPR signal in RU) were normalized to a baseline value of All sensorgram...
... survival Caspases, a family of cysteine acid proteases, are central regulators of apoptosis [12,13] Caspases are routinely used asa measure of apoptosis, in contrast to necrosis Caspase activation ... (Forward) TAAGCTACTACGTAAAGAATATATC (Reverse) GATAAGGTACCTGCACTGACACGGATGAAAGC (Forward) CATATATTCTTTACGTAGTAGCTTA (Reverse) FEBS Journal 272 (2005) 4091–4102 ª 2005 FEBS 4099 Molecular characterization ... occurs at the intersection of all caspase-dependent pathways and is, therefore, an excellent marker of caspase-dependent apoptotic death We sought to identify whether caspase is activated during...
... the Protein Data Bank database under the accession number 3NJT Overlay assay Channel analysis Fha30 isa 30-kDa, secreted FHA truncate encompassing the TPS domain and first three repeats It was ... blot with an anti-FhaC serum This antibody was raised in rats against the periplasmic domain of FhaC and prepared by Eurogentec (Seraing, Belgium) The amounts of Fha44 and FhaC were quantified by ... was used asa template with the following primers : R450AUp (5¢-GACGAGTACACGGT GGCCGGATACAACCTCAGGA-3¢) and R450ALo (5¢-TC CTGAGGTTGTATCCGGCCACCGTGTACTCGTC-3¢); Y452AUp (5¢-ACACGGTGCGCGGAGCCAACCTCAAG...
... single AATAAA polyadenylation signal was found 11 nt upstream ofthe poly (A) tail There was only one stop codon (TAA) at the 3¢ terminus ofthe ORF The cDNA sequence has been submitted to GenBank ... such asA (Ala) or D (Asp) Only AaHIT4 and BmKAS, a specific anti-insect toxin, also contained a Tyr residue at this position AaHIT4, the unique anti-insect toxin also has a toxic effect on mammals ... treatments has not been established The mechanisms underlying these alterations in cardiovascular function remain unclear It has been suggested that the cardiovascular effects of scorpion venom are dependent...
... the action ofthe cilia that continue to clear mucus toward the natural ostium It is possible that theforeign body dislocated near the maxillary natural ostium created an antral inflammation of ... symptoms of maxillary sinusitis including tenderness in the left infraorbital region and nasal stuffiness In this case a small bone window in the lateral wall ofthe maxillary sinus was performed ... view oftheforeign body in the supero medial aspect ofthe maxillary sinus Intraoperative endoscopic view oftheforeign body in the supero medial aspect ofthe maxillary sinus Figure Intraoperative...
... CGCGCCTCGAGTTAAGCTGCACCTCCTGATCT GCGCGGATCCACCATGCCCTTCCGGAGAAGA CGCGCCTCGAGTTAAGCTGCACCTCCTGATCT GCGCGGATCCACCATGTCCTTGAGGGACACA CGCGCCTCGAGTTAAGCTGCACCTCCTGATCT GCGCGGATCCACCATGGCACTTAACCCATG CGCGCCTCGAGTTAACGTTCTGTTGGTGAGCT ... GCGCGGATCCACCATGGCACTTAACCCATG CGCGCCTCGAGTTAACGTTCTGTTGGTGAGCT GCGCGGATCCACCATGGCACTTAACCCATG CGCGCCTCGAGTTATGGAGTTGAACTAGCTGT GCGCGGATCCACCATGTCCTTGAGGGACACA CGCGCCTCGAGTTAACGTTCTGTTGGTGAGCT Length (bp/amino acids) 576/192 ... Oligomerization in the context of thermotolerance, as described in this study, isnotas thoroughly investigated asthe association between quaternary structure and chaperoning in vitro, where an increase...
... CTCTCCTGAGCACACTCACAGCTGTTAACGCTG ACATCA-3¢ and Back EGT 5¢-GATCTGATGTCAGCG TTAACAGCTGTGAGTGTGCTCAGGAGAGCGAG CCAACATAAGATGGTCATGGTGGCG-3¢ (In order to avoid homologous recombination between the two ... a terminal Gal residue of asialofetuin and asialo -a1 -acid glycoprotein As shown in Table 1, the best acceptor substrate of hST6Gal II was the free disaccharide Galb1–4GlcNAc, lacto-N-neotetraose ... data), asthe template and two specific primers For 6I 5¢-CGATGAATTC GTTAACGCTCATCACCATCACCATCACGGGAAA TTGGCCATGGGGT-3¢ containing a HpaI site and Back 6I 5¢-CGATGGTACCGTACTTGTTCATGCTTAGG-3¢ and...
... TSVSAGDGAFGNLAAALTLVEDTEDGLGVKTKNGGKGFSEGTAAISQTAGANGGATVKKA VSASAANGFFKNLGKATTEVKTTKDGTKVKTKTAGKGKTGGTATTIQIADANGGVSEKSL AAAAAGNGVFKNLVTALTNISTTDDITKVQTQTIGSGGTGGAATILQLADANGGAALKEV Mrcp19k Bacp19k ... contact with the material surface The adsorption to alkylated gold was two-thirds of that to bare gold It isnot clear from this study whether this was due to an enlarged contact area ofthe ... Chemical Industries (Osaka, Japan) and Takara Shuzo Co (Otsu, Japan) Twofold-concentrated ASW was prepared by dissolving ASW (Senju Seiyaku Co., Osaka, Japan) in ultrapure water, which was ultrafiltered...
... realisation ofthe database, as well asthe consolidation ofthe both databases and the unlimited operationofthe European database b Cooperation with the other PASS -NET partners This secures the internationality ... International Passivhaus database actors Establishing ofa platform for know-how transfers of international Passivhaus actors trough: a Core-cooperation ofthe IG Passivhaus Austria and the Passivhaus ... innovation and technology In particular, the database is to serve as an international acknowledged database for decision-makers ofthe European Commission and the European Parliament as well as all...
... TCCATGTCACCTTTAAGGCAGAG-3¢ and 5¢-ATAT CCCGGGTTAAAATTTAAACAATCCTG-3¢ N-terminal deletion, ABZ1(47–138): 5¢-TATGGATCCATGC TTCTGCAAGATTTGACAGG-3¢ and 5¢-ATATCCC GGGTTAAAATTTAAACAATCCTG-3¢ C-terminal deletion, ABZ1(1–100): ... 5¢-TATACTCGAGATGTCACCTTTAAG GCAGAG-3¢ and 5¢-ATATCCATGGGCTTCTTCATC CTCGATCGC-3¢ Abz1(1–24): 5¢-TATACTCGAGAT GTCACCTTTAAGGCAGAG-3¢ and 5¢-ATATCCATG GTCTCATCCATTCCTGCATAC-3¢ Abz1(45–138): 5¢TATACTCGAGATGCAGAAGCTTCTGCAAGATTT ... PCR amplified from a full size cDNA clone using the following primer pairs Abz1(1–138): 5¢-TA TACTCGAGATGTCACCTTTAAGGCAGAG-3¢ and 5¢-ATATCCATGGAAAATTTAAACAATCCTGATG-3¢ Abz1(1–44): 5¢-TATACTCGAGATGTCACCTTTAAG...
... bethe case This may be relevant to the calculation of smelt AFP activity The activity of smelt AFP is about one-third of that of sea raven AFP on a monomer concentration basis [11] As sea raven ... concentrations of BS3, the disappearance ofthe monomer band corresponded to the gradual increase in intensity ofa higher-molecular-mass band The apparent molecular mass ofthis band was 38 kDa, ... Asthe molecular mass ofthe protein on SDS/PAGE is 22 kDa, the value obtained by gel filtration is consistent with a dimer There was no evidence of larger aggregates, as determined by absorbance...
... expressed at the beginning ofthe exponential growth phase and regulates the transcription ofthe ribosomal genes, among others On this basis, Vora et al [3] hypothesize that tsEPODs may comprise the ... should be to obtain chromosomal occupancy profiles at different growing phases - that is, lag, early, mid, and late exponential and early and late stationary phases With these data, investigators ... should be able to obtain a dynamic picture of protein occupancy for NAPs along the different growth phases ofa bacterial culture As each NAP is produced maximally at different growth phases,...
... 5'-GGGTTTCATATGAAGATCGAGGTGAGAGAA-3' 5'-CGGGATCCTTAGATATCATATAGGAACTTGC-3' 5'-TCCCCAATTTTCACACAC-3' 5'-AAGTGCCGATTTTAGATAAT-3' detected whenthe chlorogenate was incubated with CoEnzyme A in presence of ... 5'-TTTTATCCNATGGCNGGDMG-3' 5'-AACGTTHCCRAARTANCC-3' 5'-ATGGCAACACTGTCAATTA-3' 5'-CCCGACGATCAGGATA-3' 5'-ACCGCCGGGATGAGTT-3' 5'-CCGCCTCCACGAACAA-3' 5'-TTCCGTTTCGTTTCTTCAA-3' 5'-TGGCCATAACCATTTTAGATAT-3' ... an Arabidopsis thaliana HCT (80% identity and 89% similarity) (Fig 2) More distantly related acyltransferases are those annotated as hydroxycinnamoyl-CoA quinate: hydroxycinnamoyl transferase...
... the last kinase in this cascade is known asthe MAP kinase, an example of which is extracellular signal-regulated kinase (ERK) (Figure 3)25 The activation ofthe MAP kinase pathway mediated by EGFR ... MAP kinase pathway The MAP kinase pathway consists ofa phosphorylation cascade that regulates gene transcription The phosphorylation cascade consists of three protein kinases, in which the last ... diagnosis103; 104 This scoring system is based on clinical and laboratory features, such asthe percentage of blast cells, the size ofthe spleen as well asthe platelet count, to assess a CML patient’s...
... of PPARγ agonists for the treatment of diabetes and the use of PPARα agonists for the treatment of dyslipidemia and coronary heart disease makes it likely that dual PPARα and PPARγ agonists might ... region ofthe PPARα has been observed in Singapore and other East-Asian populations with relatively high allelic frequencies This variant was associated with perturbations in plasma lipid levels and ... PPRE, the regulation of PPAR expression, the post-translational modifications of PPAR; and the association with coregulators 1.4.1 Action of AF-1 and AF-2 The AF-1 at the N-terminal ofthe PPAR TAD...
... However, this DNA damage-mediated apoptosis signals can be attenuated and this may bea result of resistance, which isa major limitation of cisplatin-based chemotherapy There are several mechanisms ... a key obstacle for the treatment of patients with advance ovarian cancer 1.3.3 Cisplatin Mode of Action and Molecular Basis of Resistance Cisplatin belongs to the chemotherapy group of alkylating ... treatment of ovarian cancer is based on the staging ofthe disease, which reflects the extent and spread ofthe cancer to other parts ofthe body 1.3.1 Surgery and Chemotherapy for Ovarian Cancer...
... types of catalaseperoxidase (Kat1-3) of which KatB being the major catalase enzyme (Srinivasa Rao et al., 2003b) KatB was required for E tarda survival and replication in phagocyte-rich organs ... humans E tarda isthe only species ofthis genus that can infect humans and cause diseases in humans Association of E tarda with human diseases was first reported in 1969 (Jordan and Hadley, 1969) ... speculated to help in bacterial adherence to host cells Clinical E tarda isolates were invasive in a HeLa cell assay (Marques et al., 1984) Janda et al (199 1a) reported that E tarda was able to penetrate...
... xylosoxydans ssp xylosoxydans A- 6 N-acylD-glutamate amidohydrolase; Alicaligenes faecalis-DA1: Alcaligenes faecalis DA1 N-acyl–D-amino acid amidohydrolase; V paradoxus Iso1: Variovorax paradoxus Iso1 ... and the pI was predicted to be 5.80 by the use ofthe N-D-AAase amino acid composition in the DNASIS software package Sequence comparison ofthe V paradoxus N -D-AAase protein Alignment by the ... alignment analysis ofthe other N-D-AAase protein gene sequences in the GenBank database A single band was obtained after PCR amplification with these degenerate primers using the plasmid pBK-damD4 as...
... positions the Nokia Game experience asa game; consider the paraphrase, "Really, it's a game." As opposed to the Beast, there is no real effort to disguise the game's gameness Thisis especially evident ... that nothing about this virtual play was simulated The computer-driven alternate reality the Beast created was make-believe, but every aspect ofthe player's experience was, phenomenologically ... perception of New York City's landscape yielded a merged terrain, rather than separate perceptions ofa play and a real Manhattan Although the pervasive elements ofthe Beast (phone calls, PDA downloads,...