... 2002, accepted 23 October 2002)Eur. J. Biochem. 269, 6142–6151 (2002) Ó FEBS 2002 doi:10.1046/j.1 432 -1 033 .2002. 033 30.xsubstitute for mammalian p300/CBP. Moreover, we foundthat IRF -3 interacts ... acids 37 0 bound poorly to GST-p300C, -CBP-Cor -CBP-C2, while binding to GST-p300N, -CBP-N or-CBP-N2 was very similar to that of other IRF -3 Fig. 4. IRF -3 interacts with multiple domains of p300/CBP. ... amino acids 3 1 73) , p300DE1a (D aminoacids 1 739 –1871) and p300DSRC (D amino acids 2042–2157) all completely failed to activate transcription in thepresence of IRF -3. The inability of p300DSRC...