Evaluation of cDNA libraries from different developmental stages of schistosoma mansoni for production of expressed sequence tags (ESTs)

... 233 Table Information about the sequencing of different S mansoni cDNA libraries S mansoni cDNA libraries Number Number Number Number of of of of ESTs sequenced clones usable ESTsa> usable clones'1' ... 232 ESTs from S mansoni cDNA Libraries Methodology 2.1 Construction of cDNA libraries and sequencing The following seven cDNA libraries were used in this study: four libraries (Adult 1-4) from adult ... preliminary pattern of gene expression in distinct stages of the development of S mansoni, we performed a clustering analysis, joining sequences from all libraries This resulted in a total of 466 unique...

báo cáo khoa học: " Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress" pot

... sequence tags (ESTs) between drought- tolerant and -susceptible genotypes of chickpea under terminal drought stress BMC Plant Biology 2011 11:70 Submit your next manuscript to BioMed Central and take ... transcript profiles of ICC 4958 and ICC 1882 under drought stress To identify differentially regulated transcripts in response to terminal drought stress between droughttolerant ICC 4958 and drought- susceptible ... roots of resistant genotype (ICC 4958) under drought stress Up regulated in roots of extremes bulks of RILs of HRB under drought stress Down regulated in roots of extremes bulks of RILs of HRB under...

báo cáo khoa học: " Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour" ppt

... this article as: Altenbach et al.: Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin ... the gamma gliadin protein families and sequence redundancy within the databases, peptide data were further inspected manually For each protein band, individual gamma gliadin peptides obtained from ... Butte 86 gamma gliadins by MS/MS Because neither the NCBI database nor any single EST assembly contained sequences that matched all of the Butte 86 gamma gliadins, specialized databases that included...

Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensis and the evolution of secreted proteins in parasites potx

... [53] The closest free-living taxa to the Strongylida are members of the Rhabditina, including C elegans, and both are grouped in Clade V of the Nematoda, on the basis of small subunit rRNA sequence ... existing entries), using a cutoff score of 80 in BLASTX (P < e-10) The number of ESTs bearing potential signal sequences was then calculated and the results are shown here (b) Effects of relaxing ... for parasite secreted proteins indeed acquired signal peptides, or have free-living lineages lost these motifs in the genes in question? Is more rapid diversification of secreted proteins a specific...

báo cáo khoa học: " An analysis of expressed sequence tags of developing castor endosperm using a full-length cDNA library" ppsx

... Akiyama K, Oono Y, Muramatsu M, Hayashizaki Y, Kawai J, Carninci P, Itoh M, Ishii Y, Arakawa T, Shibata K, Shinagawa A, Shinozaki K: Functional annotation of a full-length Arabidopsis cDNA collection ... The fatty acid analysis of the transformant yeast cells grown in galactose-contain- Figure (FAH12) comparison between the oleate hydroxylase Sequenceand the oleate desaturase (FAD2) in castor Sequence ... final draft Additional material Additional file BLAST results of unique castor cDNA sequences BLAST results of 1,908 unique sequences from a full-length cDNA library of developing castor endosperm...

báo cáo khoa học: " Characterization of expressed sequence tags obtained by SSH during somatic embryogenesis in Cichorium intybus L" pot

... representing genes preferentially expressed during SE, was obtained by using cDNAs from K59 mRNAs as 'tester' and cDNAs from C15 mRNAs as 'driver' The NE library was obtained by reversing 'tester' ... quality of the libraries, and their use for future research on differential gene expression during SE in chicory Results Cellular events during the induction of somatic embryogenesis in K59 and ... by expressed sequence tag database analysis Fertil Steril 2001, 76(3):550-554 Miner D, Rajkovic A: Identification of expressed sequence tags preferentially expressed in human placentas by in...

báo cáo khoa học: " Analysis of cDNA libraries from developing seeds of guar (Cyamopsis tetragonoloba (L.) Taub)" ppsx

... GUAR_ UG_03863 GUAR_ UG_06076 GUAR_ UG_00199 GUAR_ UG_00205 GUAR_ UG_00417 GUAR_ UG_05291 GUAR_ UG_05432 GUAR_ UG_05435 GUAR_ UG_05535 GUAR_ UG_05588 GUAR_ UG_05592 GUAR_ UG_05865 GUAR_ UG_06252 GUAR_ UG_06353 GUAR_ UG_06800 ... Venn diagram of unigenes detected in the "Early" (15–25 DAF) and "Late" (30–40 DAF) guar cDNA libraries (B) Distribution of unigenes from the "secure" assignment category in classes of putative ... development of guar seeds Results and Discussion Generation of cDNA libraries Figure shows sections of developing guar seeds at 25 days after flowering (DAF) and of mature seeds at 40 DAF The mature seeds...

Báo cáo y học: "n Ambystoma mexicanum EST sequencing project: analysis of 17,352 expressed sequence tags from embryonic and regenerating blastema cDNA libraries" pot

... laevis cDNA library, and for sequences from a Canis familiaris EST project Results Assessment of library and EST sequence quality To generate a diverse set of sequences involved in organizing and regenerating ... similarity Analysis and assembly of sequence data Genome Biology 2004, 5:R67 information Quality control of sequenced ESTs was performed using the program Phred [11] using a cutoff of 20 for ... standard parameters Phylogenetic analysis of mitochondrial 12S rRNA was done using the programs dnadist, phylogenetic analysis of the cyclin B family and the CDK inhibitor family (CKI family)...

Expressed sequence tags analysis of major allergens producing dust mites and molecular characterization of their allergens

... www.pdffactory.com List of Tables IgE binding rates of the cloned dust mite allergens and their biological properties 22 Overview of the productions of recombinant mite allergens: from the aspects of the production ... mutants of the mites FABP homologues 70 Overview of the characteristics and statistical data of the dust mites EST collections 80 Summary and comparisons of gene expression levels in D farinae and ... House dust mites as an important cause of allergy 1.6.1 General aspects of mites Mites are normal inhabitants in our environment and are abundant in house dust as well as barns and grain stores Mites...

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TissueSpecific Expressed Sequence Tags from the Black Tiger ShrimpPenaeus monodon

... appear to contain the 5Ј end of the gene and hence can be aligned with each other This indi- Expressed Sequence Tags from the Black Tiger Shrimp 475 cates clear differences between the sequence encoded ... Resource; SP, SwissPROT; TREMBL, translated from EMBL Expressed Sequence Tags from the Black Tiger Shrimp 473 Table Microsatellite sequences from Shrimp EST Sequences Clone Type of microsatellite ... overlap with sequences from the other libraries One of the “unknown” sequences from the pleopod library was related to one from the eyestalk library Complementary DNAs Containing Sequences of...

báo cáo khoa học: " Analysis of tall fescue ESTs representing different abiotic stresses, tissue types and developmental stages" potx

... analyzed 41,516 tall fescue ESTs from nine cDNA libraries representing different abiotic stresses, tissue types and developmental stages A large number of known stress response gene ESTs were identified ... 1: Tall fescue cDNA library and ESTs summary cDNA library namea NCBI dbEST accession No Average length (bp) No of ESTs No of TCc No of singleton No of unique TCe No of ESTs in unique TC No of ... percentage of unique ESTs, which include both singleton and ESTs in unique TCs, is based on ESTs present in each library, and therefore, suggests library specificity of ESTs Almost two-thirds of the ESTs...

EVALUATION OF THE REAL SITUATION OF IMPORT ACTIVITIES OF VIETNAM ENERGY DEVELOPMENT SUPPORT JOINT STOCK COMPANY FROM 2009 TO 2011. PROBLEMS AND SOLUTIONS.

... OF VIETNAM ENERGY DEVELOPMENT SUPPORT JOINT STOCK COMPANY As a young company in the field of import of energy -supported equipment, the Vietnam energy development import support joint stock company ... of import activities of Vietnam energy development support joint stock company in recent years 3.1 The real situation of the company from 2009 to 2011 3.1.1 The company s capital As a young company ... period, I would like to make a report on: Evaluation of the real situation of import activities of Vietnam energy development support joint stock Company from 2009 to 2011 Problems and solutions”...

Evaluation of Loading Rate of Nitrogen from Rice-Paddies by Small Watershed Method

... mineralization of organic nitrogen, as Kunimatsu et al (1991) indicated Storm-runoff loads Separate L(Q) method It is well known that loading rates of materials and flow rates - 119 - Journal of Water ... balance of TN was estimated from the results obtained from continuous observations for three years at a small watershed of rice-paddies MATERIALS AND METHODS Study site The experimental small rice-paddy ... CONCLUSIONS By combine of interval-loads method (ILM) and Separated L(Q)-equations method, the unit load of TN for rice-paddy was evaluated on the base of the data obtained from the experimental small...

Evaluation of Nutrient Loads from a Citrus Orchard in Japan

... Study Area Study area including in a citrus orchard is located in Shizuoka Prefecture, in central Japan (Fig 1) This area is famous in Japan for the production of high quality mandarin oranges ... of Japan Water Works Association., 53(2), 38-44 (in Japanese) Komai Y., Umemoto S., Inoue T and Imai A (2003) Evaluation of annual loadings of major ionic species and nutrients in forested watershed, ... load from farmland during rainfall events We can obtain sequential nutrient load data by empirical estimations made from sequential water discharge data (i.e., nutrient load is predictable from...

Evaluation of different weather files on energy analysis of buildings

... Underestimation of fuel consumption as a function of the area served by the boiler system Conclusion A large scale simulation of heating and cooling energy demands estimation, concerning educational buildings, ... Description of the weather files As already mentioned, climate data are required for energy simulation of buildings Typically, these data consist of 8760 (the hours of a year) sets of characteristic ... Assessment of the weather files The two weather files of the Thessaloniki used in this work are based on measurements of the Micra Meteorological Station, situated at the Macedonia International Airport...

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Từ khóa: necs ecs and somatic embryos of different developmental stages apos fold changes of gene expression apos are correlated with gc3 and gcg genes specifically expressed in different developmental stages bear different molecular features different growth stages of rice plant production of ethanol from sugar cane molasses and evaluation of its quality usually as a result of timing differences between taxable income and accounting profit before tax deriving from different timing criteria used to determine these two results these differences therefore reverse in subsequent periods parents evaluation of developmental status peds tên bài báo comparative evaluation of different co antioxidants on the photochemical and functional stability of epigallocatechin 3 gallate in topical creams exposed to simulated sunlight comparison of petroleum from different countries preparation of next generation sequencing libraries from damaged dna woman called back from screening for evaluation of the tiny asymmetric density in the lower portio preparation of a high quality cdna library from a single cell quantity of mrna using chum rna construction of a full length cdna library from castor endosperm for high throughput functional screening bioinformatic methods for finding differentially expressed genes in cdna libraries applied to the identification of tumour vascular targets enzymatic production of rnai libraries from cdnas and high throughput selection of effective shrna expression constructs construction of small rna cdna libraries for high throughput sequencing Nghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt nam đề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giải Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit ĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN Phối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọ Phát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninh Trả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ long Phát hiện xâm nhập dựa trên thuật toán k means Nghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùng Nghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúng Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIX Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Quản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ