construction of small rna cdna libraries for high throughput sequencing
Báo cáo y học: " A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454" pps
... of multiplexing for small- to-medium numbers of samples (for example, 20 to 40 libraries) In this method, plate-based library construction proceeds to completion as described above Completed libraries ... of sequence-ready libraries This is particularly pressing for projects that call for sequencing of large numbers of samples each requiring a modest amount of data, such as small genomes or amplicons ... any automation Settings used for plate-based shearing of DNA are: Duty Cycle of 5; Intensity of 5; Cycle per Burst of 500; Seconds of 120; Well Plate of '96 well offset + mm' It is important...
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![bioinformatics for high throughput sequencing [electronic resource]](https://media.store123doc.com/images/document/14/y/gu/small_Yb8L87hXir.jpg)
bioinformatics for high throughput sequencing [electronic resource]
... 2.1 High- throughput sequencing workflow There are three main steps in high- throughput sequencing: preparation, immobilization, and sequencing Preparation of the sample for highthroughput sequencing ... in the construction of the high- throughput sequencing instruments Commercial high- throughput sequencing platforms share three critical steps: DNA sample preparation, immobilization, and sequencing ... Bioinformatics for High Throughput Sequencing Naiara Rodríguez-Ezpeleta Ana M Aransay ● Michael Hackenberg Editors Bioinformatics for High Throughput Sequencing Editors Naiara...
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báo cáo khoa học: "Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.)" doc
... specific for G19833 derived mRNAs were used to identify the genotype of the isolated cDNA clones Library construction and EST sequencing Library construction was performed at the RIKEN institute of ... soil treatments for these two genotypes, respectively, which was sufficient for the highly complex process of full-length mRNA selection from polyA mRNAs For the libraries, a total of 20,000 clones ... Univ of Washington for sequencing The high rate of unigenes per HQ sequence is fairly unique among cDNA libraries established for common bean to date and represents the broad representation of...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... accessible This allows the synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble ... demonstrated for each of the 19 nonproline residues [18] Time and expense can be drastically reduced by use of cell-free systems [11,18,21], opening many avenues for strategic applications of selectively ... can be produced in high yields in vivo are also suitable for efficient production by cell-free synthesis In order to compensate for the increased effort and expense required for the production...
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Báo cáo khoa học: Construction of a novel detection system for protein–protein interactions using yeast G-protein signaling pdf
... The amplifier gain was set at 1.00 for forward scattering; the detector voltage was set to E00 for forward scattering and 600 V for FL1, and the threshold for forward scattering was set at 52 The ... FEBS Journal 276 (2009) 2636–2644 ª 2009 The Authors Journal compilation ª 2009 FEBS 2641 Detection system for protein–protein interactions N Fukuda et al Table Primers used for construction of plasmids ... detection of protein–protein interactions Detection of interactions in the absence of FEBS Journal 276 (2009) 2636–2644 ª 2009 The Authors Journal compilation ª 2009 FEBS 2639 Detection system for...
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Báo cáo sinh học: "Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" doc
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... response G(X) = k+X is linear for low density X (gX ≈ 1) and saturates for high density X (gX ≈ 0) So far we succeeded in expressing the Jacobian of the model as a function of three easily interpretable...
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Báo cáo hóa học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" pdf
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... response G(X) = k+X is linear for low density X (gX ≈ 1) and saturates for high density X (gX ≈ 0) So far we succeeded in expressing the Jacobian of the model as a function of three easily interpretable...
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Báo cáo toán học: " Generalized Models for high-throughput analysis of uncer- tain nonlinear systems" ppt
... Purpose — Describe a high- throughput method for the analysis of uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysis of large classes of models Results ... Generalized Models for high- throughput analysis of uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... response G(X) = k+X is linear for low density X (gX ≈ 1) and saturates for high density X (gX ≈ 0) So far we succeeded in expressing the Jacobian of the model as a function of three easily interpretable...
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Báo cáo hóa học: "Research Article Design of an Omnidirectional Multibeam Transmitter for High-Speed Indoor Wireless Communications" doc
... each of the higher order impulse responses; however, for configuration D the contributions of low-order impulse responses dominate (more than 50% for k < 2) but for configuration E that of higher-order ... (a)–(d) for LOS path, (e)–(h) for the first bounce of reflection on the wall, and (i)–(l) for the fifth bounce of reflection on the wall Table 2: Simulation parameters for five configurations of multibeam ... in joint with a high- resolution imaging angle diversity receiver power uniformity of the transmitter has yet to be optimized For high performance wireless diffuse links with uniform power illumination...
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CONSTRUCTION OF UPPER AND LOWER SOLUTIONS FOR SINGULAR DISCRETE INITIAL AND BOUNDARY VALUE PROBLEMS potx
... 1))) > implies ∆2 v(k − 1) < for k ∈ N, it follows from Lemma 1.4 that v(k) ≥ µ(k) v for k ∈ N + Thus, < α(k) ≤ n0 for k ∈ N, = c p−1 ≤ c0 −∆ ϕ p ∆α(k − 1) (2.7) for k ∈ N, α(0) = α(T + 1) = ... 1/n0 for k ∈ N + Then ∆(ϕ p (∆u(k − 1))) = −∆(ϕ p (∆v(k − 1))) ≤ for k ∈ N, and v(0) = v(T + 1) = Lemma 1.5 guarantees that v(k) ≥ and so u(k) ≥ 1/n0 for k ∈ N + Next we prove u(k) ≤ M for k ... 0, k ∈ [a,b], ≤ 0, (1.14) u(b + 1) ≥ 0, then u(k) ≥ for k ∈ [a − 1,b + 1] = {a − 1,a, ,b + 1} ⊂ N + In Theorems 1.2 and 1.3 the construction of a lower solution α and an upper solution β is critical...
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summary of thesis for phd of pedagogy educating living skill for high school students through extracurricular activities
... necessity of life skills education for high school pupils and other structural elements of life skills education for high school pupils 1.2.2.1 The necessity of life skills education for high school ... create a basis for selection of methodologies for pedagogic experiments 7 16 1.2.2.2 The structural elements of life skills education for high school pupils Life skills education for high school ... elements of out -of- class-hour educational activities, they can improve the efficiency of life skills education for high school pupils 5.Tasks of the research 5.1 Systematize theoretical issues of life...
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Báo cáo khoa học: " Guidelines for delineation of lymphatic clinical target volumes for high conformal radiotherapy: head and neck region" potx
... 6:97 http://www.ro-journal.com/content/6/1/97 Page 20 of 25 Table Suggested guidelines for the treatment of the neck of patients with carcinomas of the skin Skin of scalp forehead submental (IA) ... cell tumors of the nasopharynx show a very high rate of NM in 80% of the patients [7] Even for N0 patients the incidence of NM in the bilateral level IIA, IIB, III, IV, VA and VB is high and should ... detected NM of level VA in pN0 patients with a prevalence of 2.3% and in pN+ patients with 16.7% The prevalence was highest for tumors of the pharynx (23.1%) in contrast to tumors of the oral...
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Báo cáo khoa học: " A dosimetric comparison of four treatment planning methods for high grade glioma" pptx
... cm3) respectively A mean of >98% (range 92-100%) of the PTV1 received 100% of the prescribed dose in all planning methods A mean of 95.5% and 95.7% of the PTV2 received 100% of the prescribed dose ... with the high conformality and a rapid fall off of the dose around the target volumes had the lowest D10 to the normal brain (only a small volume around the target volume received a high dose) ... volumes [12-14] We added an analysis of three points in the DVH curve (D10, D50 and D90) of the normal brain to evaluate the validity of the ID as a tool for this kind of comparisons since different...
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Báo cáo y học: "miRTRAP, a computational method for the systematic identification of miRNAs from high throughput sequencing data" pptx
... product displacement; miRNA/miR: microRNA; miRTRAP: miRNA Tests for Read Analysis and Prediction; moR: microRNA-offsetRNA; piRNA: Piwi-interacting RNA; siRNA: small interfering RNA; UTR: untranslated ... J, Hoffmann S, Khaitovich P, Stadler PF: Evidence for human microRNA-offset RNAs in small RNA sequencing data Bioinformatics 2009, 25:2298-2301 Yousef M, Showe L, Showe M: A study of microRNAs ... types of small RNAs This may reflect the large size of pri-miRNA transcription units with strict secondary structures to produce miR hairpins Except for the case of antisense miRNAs or miRNAs...
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Báo cáo y học: "ISsaga is an ensemble of web-based methods for high throughput identification and semiautomatic annotation of insertion sequences in prokaryotic genomes" pdf
... For example, for an IS composed of two ORFs, ISsaga will extract the nucleotide sequence starting from the coordinates of the beginning of the first ORF to the coordinates of the end of the second ... introduced a number of rules that operate automatically to remove many of the major annotation ambiguities encountered due to the diversity and complexity of ISs (for example, the presence of more than ... Exploitation of ISsaga Genome context One useful feature of ISsaga is that it supplies the genome context (that is, flanking genes) for each annotated Page of IS, allowing identification of IS-induced...
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báo cáo khoa học: "In Medicago truncatula, water deficit modulates the transcript accumulation of components of small RNA pathways" pptx
... small RNA world Journal of cell science 2003, 116:4689-4693 Szittya G, Moxon S, Santos DM, Jing R, Fevereiro MP, Moulton V, Dalmay T: High- throughput sequencing of Medicago truncatula short RNAs ... of 21-nt that corresponds to the miRNA [2,3] and a faint band of 24-nt The probable reason for the extra bands is that DCL3 competes with DCL1 for the same miRNA precursors to produce small RNAs ... enzymes, RNA- dependent RNA polymerase (RDR6), Suppressor of gene silencing (SGS3) and Silencing Defective (SDE5) are involved in double strand RNA (dsRNA) production from the cleaved mRNA of AGO1...
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báo cáo khoa học: " Construction of a consensus linkage map for red clover (Trifolium pratense L.)" docx
... Next, the robustness of the data sets for each linkage group was confirmed by the grouping module of JoinMap using an logarithm of odds (LOD) threshold of 2.0 For the construction of a consensus linkage ... consensus linkage map for red clover The locus order of the present consensus map is highly consistent, and is sufficiently reliable for use as a reference for the genetic analysis of random red clover ... goodness -of- fit Jump threshold for removal loci = 5.0, number of added loci after which to perform a ripple = 1, and third round = Yes A total of eight individual maps were developed for HR, R130,...
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Báo cáo y học: "Establishment of a novel CCR5 and CXCR4 expressing line which is highly sensitive to HIV and suitable for high-throughput evaluation of CCR5 and CXCR4 antagonists" pdf
... concentrations (IC50) of SCH-C for inhibition of LD78β-induced calcium flux were ng/ml in the single and ng/ml in the double-transfected cells For AMD3100, the IC50 values for inhibition of SDF-1-induced ... after infection of cells The coreceptor use of each isolate is shown between brackets The data of one representative experiment out of two are presented Page of 13 (page number not for citation ... vitro models is a prerequisite for the successful setup of an accurate screening program for chemokine receptor antagonists Here, we Page of 13 (page number not for citation purposes) Retrovirology...
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guide for the design and construction of externally bonded frp systems for strengthening concrete structures
... the transformed section analysis To avoid inelastic deformations of the reinforced concrete members strengthened with external FRP reinforcement, the DESIGN AND CONSTRUCTION OF EXTERNALLY BONDED ... moment of the areas of the transformed section The transformed area of the FRP may DESIGN AND CONSTRUCTION OF EXTERNALLY BONDED FRP SYSTEMS 440.2R-25 Fig 10.1—Typical wrapping schemes for shear ... Innamorato, D., 1997, “Seismic Retrofit of RC Columns with DESIGN AND CONSTRUCTION OF EXTERNALLY BONDED FRP SYSTEMS Continuous Carbon Fiber Jackets,” Journal of Composites for Construction, No 1, pp 52-62...
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Study of bit patterned fept media for high density magnetic recording
... requirement of planarization of the disk surface The Gaussian peak of the implantation profile in such cases lies at the center of the recording layer and requires the usage of high energy values of a ... inexpensive and provide high- throughput for patterning high density nanostructures (of dimensions
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25