báo cáo hóa học: "Measuring patient''''''''s expectation and the perception of quality in LASIK services" pdf
Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... located in the C-terminal loop of SNase playsan important role in protein stability and conforma-tional integrity. During protein unfolding ⁄ refolding, the addition of 2.5 protons (both E75 and ... role in maintainingSNase tertiary structure.Our CD and DSC data show that the W140 in SNase is the amino acid responsible for the stability of the whole protein. However, in comparison with the wild-type ... SNase.Changes in the fluorescence intensity of W140 reflecta change in the hydrophobic environment surroundingW140 and thus indicate a change in the overall (ter-tiary) structure of the protein. Figure...
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Tài liệu Báo cáo khoa học: "What lies beneath: Semantic and syntactic analysis of manually reconstructed spontaneous speech" pdf
... concep-tion of transformation (Harris, 1957). In this view, the original verbatim string is the surface form of the sentence, and as in linguistic theory should beconstrained in some manner ... modifying material.Reconstructive insertions751Proceedings of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 746–754,Suntec, Singapore, 2-7 August 2009.c2009 ACL and ... text. In Proceedings of the AnnualMeeting of the European Association for Computa-tional Linguistics.Erin Fitzgerald. 2009. Reconstructing SpontaneousSpeech. Ph.D. thesis, The Johns Hopkins...
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Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot
... diagram of the trunca-tion mutants of CcCac1L. (C) Interaction between CcCac1L and CcLim15 in a yeast two-hybrid assay. The inserts in the activation domain(AD) and DNA-binding domain (BK) ... washing, the fractions were again collected with20 mL of a linear gradient of 0–0.5 m NaCl in buffer B.Fractions containing the recombinant proteins were verifiedby SDS-PAGE, pooled and then ... accumu-lating evidence regarding the role of CAF-1 in chroma-tin assembly following various DNA synthesis events,its involvement in chromatin assembly following mei-otic recombination is unknown. In...
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Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
... near the flexible C terminus of SNase.Changes in the fluorescence intensity of W140 reflecta change in the surrounding hydrophobic environment of the tryptophan and thus indicate a change in the overall ... (DCp) and difference of DCp of mutant from wild-type (%) were determined and the results for the wild-type protein and 20mutants are given in Table 1. The DHcal and Tm of the wild-type protein ... at a heatingrate of 1 °CÆmin)1. The melting point (Tm) was obtaineddirectly from the DSC curve. The enthalpy change (DHcal)was calculated by the integration of the curve covering area(Tmwas...
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Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot
... that in the tumoral hepatic cell line HepG2, HePC hinders the incorporation of radiolabelled choline into PC, the end-product of the CDP–choline pathway. This reduction in the incorporation of ... metabolites. We analysed the incorporation of radioactive choline into PC and sphingomyelin (SM), and also into soluble intermediaries of the CDP–choline path-way. The different phospholipids ... PCbiosynthesis. In the present work we exposed the cells toHePC between 5 min and 6 h and did not observe anychange in the uptake of choline, thus demonstrating that in this cell line at least the...
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Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc
... metabolism in the cold. Mathemati-cal modelling and simulation of cold-induced metabolic reprogrammingindicated major differences in the rates of interconversion between the pools of hexoses and sucrose, ... assessment of the trajec-tory of interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) lineup of the accessions ... concerning sucrose cycling, weperformed in silico experiments, using the validatedmathematical model in terms of predictive metabolicengineering [18]. Replacing Vmaxvalues and k values in the...
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Tài liệu Báo cáo khoa học: a-Conotoxins as tools for the elucidation of structure and function of neuronal nicotinic acetylcholine receptor subtypes doc
... purpurascens13was again identified in acloning approach making use of the high conservation of the 3¢ untranslated region and the intron preceding the sequence of the a-prepropeptide [39]. The peptide ... subunits, and was not reliably expressed in combination with b4 subunits, an a6/a3 chimera consisting of the extracellular ligand-binding domain of the a6 subunit and the transmembrane and intracellular ... subtypeselectivity, and ultimately may lead to the engineering of a-conotoxins with tailored selectivity.Information on the binding mode of neuronally activea-conotoxins and the factors that determine subtypeselectivity...
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Tài liệu Báo cáo khoa học: "Prosodic Aids to Syntactic and Semantic Analysis of Spoken English" ppt
... purpose of utteranc- es and their domain context, it is then possible to correct some of the insertion and mis-recognised word errors from the SR and determine the com- municative intent of the ... rules of the form A -> B C to the form A -> B Link C, and add con- straints to the rules application in terms of the value of the =breaking indices" based on relative duration of ... segmentation in dialogue analysis. Subse- quent work will include the other features, and further refinement of the use of pitch and pause. At present, for example, we do not consider the length of...
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Tài liệu Báo cáo khoa học: "Phrase-Based Backoff Models for Machine Translation of Highly Inflected Languages" docx
... Kneser-Neysmoothing and interpolation of trigram and bigramestimates and was trained on the English side of the bitext. In the first pass, 2000 hypotheses aregenerated per sentence. In the second pass, the seven ... of issues.BA CKOFF: we are in the durcharbeiten procedures, and we have tried to make a few streamlining of the text in anumber of points.REF: this is how we came to go through the text, and attempted ... YangDepartment of Electrical EngineeringUniversity of Washin g tonSeattle, WA, USAyangmei@ee.washington.eduKatrin KirchhoffDepartment of Electrical EngineeringUniversity of Washin g tonSeattle,...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... the first amino acid the residue preceding thismethionine is shown in lower case. The sequencecorresponding to the N-terminal sequence of the protein is underlined. The arrowheads indicate the positions ... for the b-amylasefrom C. sepium using the X-ray coordinates of the soybeanb-amylase (Fig. 4). According to the Ramachandran plot of this model the f and c angles of most of the residues are in the ... AGdinucleotides at their 50 and 30boundaries, respectively, and were inserted between the third letter of one codon and the first letter of the following codon.Molecular modeling of the C....
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