Báo cáo khoa học: Identiﬁcation of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis potx

Tài liệu Báo cáo khoa học: Identiﬁcation, sequencing, and localization of a new carbonic anhydrase transcript from the hydrothermal vent tubeworm Riftia pachyptila docx

... Histidine residues involved in zinc binding in the catalytic site are indicated by a ‘Z’; important amino acids involved in the hydrogen bond network are indicated by anasterisk; framed amino ... side of eachﬁlament (Fig. 2D), a homogenous ﬂuorescence wasobserved in the cytoplasm of the cells. The stainingappears to be speciﬁc of the probe sequence because the staining is clear with the ... trophosome-speciﬁc proteins. Wedemonstrated the existence of carbonic anhydrase transcripts, a protein endowed with an essential role in generating the in ux of CO2required by the symbionts. One of the transcripts...

Báo cáo khoa học: Identiﬁcation and functional expression of a second human b-galactoside a2,6-sialyltransferase, ST6Gal II docx

... Galb1–4GlcNAc-Rdisaccharide sequence linked to a protein. The a2,6-linkagewas conﬁrmed by the increase of Sambucus nigra agglutinin-lectin binding to the cell surface of CHO transfected with the cDNA encoding ST6Gal ... activity of hST6Gal II The acceptor substrate speciﬁcity of ST6Gal II was deter-mined using a Flag-tagged soluble form of the enzymemissing the ﬁrst 33 amino-acids. The results of the enzymatic ... the other hand, the presence of CREB and SREBP binding sites upstream exon Awould indicate a speciﬁc expression of exon A containing Fig. 8. Comparison of the putative amino-acid sequence of...

Báo cáo khoa học: Identiﬁcation and functional characterization of a novel barnacle cement protein pptx

... only in the basal portion of the animal,where the histologically identiﬁed cement gland is located. The sequence of the protein showed no homology to other known proteins in the databases,indicating ... analysis of the primary cement, and of the protein fractions in the calcareous base andperiphery, indicated that Mrcp-19k was present in the primary cement and protein fraction in the calcareousbase, ... surface of the protein are known to be essential in the process of binding to the ice nucleus [18,19], although the exactroles of these amino acids are not yet clearly under-stood. The requirements...

Báo cáo khoa học: Identiﬁcation and expression analysis of an IL-18 homologue and its alternatively spliced form in rainbow trout (Oncorhynchus mykiss) doc

... IL, interleukin; IL-1Ra, interleukin 1 receptorantagonist; ICE: interleukin 1b converting enzyme; IL)18 BP,interleukin 18 binding protein; IFN, interferon; c-IP10, interferongamma induced protein ... Cloning and expression of interleukin-18binding protein. FEBS Lett. 445, 338–342.32. Novick, D., Kim, S.H., Fantuzzi, G., Reznikov, L.L., Dinarello,C.A. & Rubinstein, M. (1999) Interleukin-18 ... spleen, using TRIZOL (Invitrogen) according to the manufacturer’s instructions. The RNA was then reversetranscribed into cDNA using Bioscript (Bioline) accordingto the manufacturer’s instructions....

Báo cáo khoa học: Identiﬁcation and biochemical characterization of the Anopheles gambiae 3-hydroxykynurenine transaminase pot

... glyoxylate,were obtained by adding increasing concentrations of the molecule to the reaction samples containing 5 mmd,l-3HKas the amino donor. The amount of XA produced after a5-min incubation was ... bymeasuring the amount of XA produced following the incuba-tion of the enzyme with d,l-3HK as amino group donor in the presence of different a-ketoacids as amino group accep-tors, according to ... 396-amino acid long protein, repre-senting the A. gambiae 3-HKT.Expression proﬁle of the 3-HKT gene The expression of the Ag-hkt gene was analysed byRT-PCR ampliﬁcation of the corresponding...

Báo cáo khoa học: Identiﬁcation, cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome pot

... form of the protein. The reduced, dithiol form of thioredoxin canmodulate the activity of a variety of target proteins byreduction of their disulﬁde bonds. Plants contain severalforms of thioredoxin ... matched the predicted mass of the protein lacking the N-terminal methionine and with the active-site cysteines in oxidized form (12 621.49 Da). N-Terminal sequencingconﬁrmed that the N-terminal ... pI in both cases.Activity of thioredoxin h isoforms Insulin reduction. The recombinant barley thioredoxin h isoforms were analysed for their ability to reduce insulin [34]and compared with the...

Báo cáo khoa học: Identiﬁcation and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus inﬂuenzae pptx

... of H-3/H-41H-resonance of the GlcNAc residue in the NOESY step. (A) The assignments of the 1H-resonances of the Gal II residue are indicated. (B) The assignments of the 1H-resonances of the ... again perhaps indicating that stericinterference of the two chains precludes coincident expres-sion of both. It is noteworthy that the majority of Sial-lNnT-containing glycoforms contain two ... residues of the Sial-lNnT unit(Fig. 7). This conﬁrms that a terminal Sial-lNnT unit islinked to the Hep I residue of the LPS inner core in the sialylated glycoform. The structure of the sialylated...

Báo cáo khoa học: Identiﬁcation and functional characterization of an aggregation domain in long myosin light chain kinase ppt

... (2006)Microﬁlament-binding properties of N-terminalextension of the isoform of smooth muscle long myosinlight chain kinase. Cell Res 16, 367–376.Aggregation domain in myosin light chain kinase W C. ... of 4Ig aggre-gates. To investigate whether other ingredients existed in the aggregate, we stained the aggregates in cells withphalloidin, and this revealed the presence of strongactin-staining ... enhanced green ﬂuorescent protein; GFP, green ﬂuorescent protein; HSP, heat shock protein; L-MLCK, long smooth muscle myosin light chain kinase; MLCK, myosinlight chain kinase; RCR, respiratory...

Báo cáo khoa học: Identiﬁcation of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis potx

... Journal compilation ª 2008 FEBS 4489 Identiﬁcation of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis Jose´R. Bardales1, ... 2008)doi:10.1111/j.1742-4658.2008.06591.xSeveral isoforms of the cAMP-dependent protein kinase catalytic subunit (C -subunit) were separated from the posterior adductor muscle and the mantle tissues of the sea mussel Mytilus galloprovincialis ... musselM. galloprovincialis. ResultsSeparation of different isoforms of the C -subunit In order to demonstrate the presence of different iso-forms of the PKA C -subunit in mussels, the protein was...

Báo cáo khoa học: Identiﬁcation and characterization of multiple species of tenascin-X in human serum doc

... deletion of either the EGF repeats orﬁbrinogen domain negatively in uenced the effect of TNX on collagen ﬁbrillogenesis, whereas they showedthat deletion of these domains did not in uence bind-ing ... that deletion of the EGF repeats, ﬁbrin-ogen domain or both resulted in a loss of binding tocollagen type I, contradicting the results by Minami-tani et al. [18,21]. Furthermore, Minamitani et ... pro-teins to the FNIII29 of TNX in a study of C-terminalTNX domains [20]. The recombinant TNX domains in our previous study were produced in prokaryotic cells[20], therefore glycosylation may in uence...

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