Báo cáo khoa học: Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN ppt
Báo cáo khoa học: Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN ppt
... phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN Philipp Schla¨fli*, Juliane Tro¨ger*,, Katrin Eckhardtà, Emanuela Borter§, Patrick Spielmann and Roland ... regulation in the pancreas. According to the current model, binding of a putative ligand to the PAS domain disinhibits the kinase domain, lead-ing to PASKIN autophosphorylation and increased kinase ... against S6 and the GST-tag. (C) Immunoblot analysis of the phosphorylation status of p70S6K and S6 in Paskin + ⁄ + and Paskin ) ⁄ )MEFs. (D) Immu-noblot analysis of the phosphorylation status of p70S6K...
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Báo cáo khoa học: Substrate recognition and ®delity of strand joining by an archaeal DNA ligase docx
... covalentaddition of AMP to the conserved AMP-binding site lysine of the protein. In the second step, the AMP i s transferredfrom the protein to the 5¢ phosphate group of the nick on the DNA t o form ... Materials and methods. (A) Ligation of 3¢ matched and 3¢ mismatched substrates. The substrates used are indicated at the top. (B) Ligation of 5¢ matched and 5¢ mismatched substrates. The substrat ... notcomplementary to any of the substrate oligonucleotides. No inhibition of the ligase reaction c ould b e observed in the presence of duplex DNA (Fig. 3B). Our results support the theory that LigTkdoes...
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Báo cáo khoa học: Substrate recognition by three family 13 yeast a-glucosidases Evaluation of deoxygenated and conformationally biased isomaltosides pptx
... occur in the segments that connect the b strands to the a helices of the (b/a)8barrel fold [ 14]. The catalytic site is located at the bottom of a cleft between domain B and several of the b ® ... between the catalytic nucleophile and the C-1 of the substrate glycon [18,30,31,45±49]. Further kineticsanalyses are not feasible due to the limited amounts of analogs available; we therefore ... essentially in the Km and not in the rate of hydrolysis as for the a-glucosidase [40]. This distinct preference for one of the two diastereoisomers of the C-6alkyl isomaltose derivatives re¯ects the fact...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
... potentials for the surface of the CPR FMN domain (in the region of the solvent-exposed FMN) with that of a homology model of the MSR FMN domain (based on the structure of the CPR FMN domain; Protein ... inset). The absorptionspectrum of the purified enzyme was typical of the hydroxycobalamin form of the enzyme (Fig. 2). The recovery of the activity was 10%, and the enzymewas purified 3669-fold. The ... of the surface of the CPR FMN domain and of a model of the FMN domain of human MSR.Fig. S3. Electrostatic potentials of the surface of the hMS AD.Table S1. Sequences of oligonucleotides used for...
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Báo cáo khoa học: Calcium, mitochondria and oxidative stress in neuronal pathology Novel aspects of an enduring theme pdf
... whichis the rate-limiting step of the TCA cycle providesNADH as a substrate for the respiratory chain complex.It is difficult to establish the extent of contribution of KGDHC and other enzymes ... (b) loss of cytochrome c impairs the flow of electrons in the respiratory chain inducing over-reduction of the complexes, favouring the generation of ROS [16,17,22]; (c) reduction in the matrix ... both in the presence and in the absence of pyridine nucleotides. Still, the reduced Dld subunit is the most likely source of ROS under conditions of anelevated NADPH ⁄ NADP+ratio in the mitochondrialmatrix...
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Báo cáo khoa học: "Capturing Paradigmatic and Syntagmatic Lexical Relations: Towards Accurate Chinese Part-of-Speech Tagging" pdf
... tag-ging. Table 3 shows the tagging accuracies relativeto the length of the spans. We can see that with the increase of the number of words governed by the token, the difficulty of its POS prediction ... overlapping and the combination of themgives more.Table 10 shows the performance of different sys-tems evaluated on the test data. The final result isremarkable. The word clustering features and the Bagging ... features and new cluster-based features; the number is the total number of the clusters; the sym-bol “+” in the Data column means which portion of the Gigaword data is used to cluster words; the...
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Báo cáo Y học: Substrate selectivity and sensitivity to inhibition by FK506 and cyclosporin A of calcineurin heterodimers composed of the a or b catalytic subunit potx
... shows that in the inactive state, the CaN autoinhibitory domain lies over the catalytic site[20]. The amino-acid sequences of the CnAa and CnAb catalytic domains are 90% identical, and the autoinhibitory domain ... inCnB to the active site [22]. In fact, the catalytic activity of CaN is sensitive to the amino-acid composition of the regionlinking the CnB-binding helix to the b14 strand of the catalytic ... exactly spans the length of the central helix of CaM, and led to the proposal that the 11consecutive Pro residues of CnAb may modulate the interaction of CaM with the CaM-binding domain of CnAb[41]....
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Báo cáo khoa học: M1 – Peptidomimetics and Signal Transduction Inhibition potx
... investi-gate the ability of various metals to induce alterations in the struc-ture of beta-amyloid (1–40), and correlate the observed resultswith the status of the peptide molecule in solution and in the ... internalization capacity of the targetcells, and cellular handling which determines the fate of the drugin the cell. Examples of vascular drug targeting systems will bepresented and their effects will ... relationship of the chemotactic ligands and the carrier molecules for CDT; (ii)to characterize cell physiological properties of the new conjugates and (iii) to describe the ability for CDT of the conjugate...
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Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt
... resulted in 55% and 51%loss of the initial activities of BHLmL2 and BHAb,respectively. These results strongly suggest that Ser isinvolved in the catalytic activity of BHLmL2 and BHAb and are consistent ... 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH on the activities of the BHenzymes was investigated in the pH range 6–11. The pH optima were determined to be in the basic range(pH ... and BHAb. The temperature dependence of the activities of the BHs was tested in the range 5–56 °C; the apparentoptimum temperatures of BHLmL2 were 25–30 °CTable 3. Masses and scores of tryptic...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
... transporter to the internal membrane side is the rate-limiting s tep and not – as proposed for most of the other electrogenic symporters – the return of the unloadedtransporter to the outside of the membrane ... differentorientation of the substrate w ithin the substrate bindingsite of PAT2, and this may simultaneously avoid the interference of the polar side ch ain w ith c orrespondingamino acid residues in the binding ... for a restrictedvelocity in the translocation step of the loaded transporterby the sterical conformation of the substrate. The introduction of polar side groups in the aliphatica-amino acids...
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