Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... FEBS Characterization of 1 H NMR detectable mobile lipids in cells from human adenocarcinomas Anna Maria Luciani 1 , Sveva Grande 1 , Alessandra Palma 1 , Antonella Rosi 1 , Claudio Giovannini 2 , Orazio ... 1–12. 8 Santini MT, Romano R, Rainaldi G, Ferrante A, Motta A & Indovina PL (2006) Increases in 1 H -NMR A. M. Luciani et al. 1 H NMR of mobile lipids...

Ngày tải lên: 18/02/2014, 13:20

... method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. Characterization of a Serratia proteinase K-like enzyme A. ... bolded; N-terminal residues of the catalytic domain are under- lined. The preregion is indicated in red, the pro-region in black, catalytic domain in blue and the C-terminal dom...

Ngày tải lên: 19/02/2014, 07:20

... transduction in T -cells [5]. Lipid rafts obtained from resting T -cells are enriched in Src- family kinases, Lck and Fyn [6,7], and the linker for the activation of T -cells (LAT) [8]. Minor amounts of CD3f ... modifying h-toxin (per- fringolysin O), a cholesterol-binding, pore-forming cytolysin produced by Clostridium perfringens [18]. Ch and BCh are noncytolytic derivatives o...

Ngày tải lên: 20/02/2014, 03:20

... protein with the N-terminus YKL) [also known as human cartilage glycoprotein-39 (HC-gp39) in humans] are considered to be cytokines [10,11] involved in tis- sue remodelling during pathological conditions ... FEBS from the oyster Crassostrea gigas [5]. Interestingly, this protein, named C. gigas chitinase-like protein 1 (Cg- Clp1) was found to be involved in the control of growth a...

Ngày tải lên: 19/02/2014, 00:20

... lines indicate intron 6 of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron 5 in fragments C and D, and 3¢ portion of 25 bp in intron 6 in fragments F and I. The number at the right of ... genera- tion of protein isoforms differing in amino acid sequence. Human DNASE1 pre-mRNA is transcribed from different transcription start sites, exons 1 and 1a, resulting...

Ngày tải lên: 19/02/2014, 06:20

... C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22], indicating that the PM H + -ATPase binding site of PPI1 is localized therein. ... blue-light activation of PM H + -ATPase in guard cells of broad beans involves protein kinase-mediated phosphoryla- tion of Ser and Thr residues in the C-terminus of th...

Ngày tải lên: 19/02/2014, 07:20

... of all of these genes remains to be clarified. Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... chemicals including amino acids, nucleic bases and vitamins were obtained from commercial sources. Acetylpyridine adenine dinucleotide (APAD) and 6-diazo-5-oxo- L -norleucine (DO...

Ngày tải lên: 19/02/2014, 12:20

... 30 min. Thereafter, 0.2 mL of serum free RPMI 1640 medium con- taining 2 · 10 6 HEL cells were added and incubated for 4 h at 37 °CinaCO 2 incubator, after which 2 mL of RPMI 1640 containing ... of Oct-2, but not Oct-1, in HEL cells and supershift assays employing anti-(Oct-2) IgG further confirmed Oct-2 binding to the Oct-1 ⁄ 2 site of Prm3. Owing to the absence of Oct-1 expre...

Ngày tải lên: 19/02/2014, 16:20

... anthocyanin sequestration, is a flavonoid- binding protein. Plant Physiol 123, 1561–1570. 24 Grotewold E (2004) The challenges of moving chemicals within and out of cells: insights into the transport of plant ... sulfo- bromophthalein to rat and human ligandins: characteri- zation of a binding-site peptide. Biochim Biophys Acta 955, 296–300. 52 Gregus Z & Klaassen CD (1982) Role...

Ngày tải lên: 20/02/2014, 01:20

... leukocyte b 2 integrins. The a chains of the CD11/CD18 integrins contain an inserted approxi- mately 200-amino-acid intervening domain (I domain), which is homologous to the A domains of von Willebrand factor, ... domains. The efficient inhibition of the Fig. 10. Specific binding of purified recombinant I domain GST fusion proteins to ICAM-4Fc in a solid phase assay. Dose-dependent bin...

Ngày tải lên: 20/02/2014, 11:20