(Luận văn thạc sĩ) phân tích vai trò của các yếu tố điều hòa hoạt động (CRE) trên vùng promoter chuyên biệt hạt phấn lúa

ĐẠI HỌC THÁI NGUYÊN TRƯỜNG ĐẠI HỌC NÔNG LÂM TRIỆU BÍCH HUỆ PHÂN TÍCH VAI TRỊ CỦA CÁC YẾU TỐ ĐIỀU HÒA HOẠT ĐỘNG (CRE) TRÊN VÙNG PROMOTER CHUYÊN BIỆT HẠT PHẤN LÚA Ngành: Công nghệ Sinh học Mã số ngành: 42 02 01 LUẬN VĂN THẠC SĨ CÔNG NGHỆ SINH HỌC Người hướng dẫn khoa học: PGS.TS Nguyễn Tiến Dũng Thái Nguyên – 2022 download by : skknchat@gmail.com i LỜI CAM ĐOAN Tôi xin cam đoan cơng trình nghiên cứu khoa học độc lập riêng tơi nhóm nghiên cứu hướng dẫn khoa học PGS.TS Nguyễn Tiến Dũng Các nội dung nghiên cứu, kết luận văn trung thực chưa cơng bố hình thức trước Tơi xin hồn tồn chịu trách nhiệm nội dung luận văn Thái Nguyên, ngày 08 tháng năm 2022 Học viên Triệu Bích Huệ download by : skknchat@gmail.com ii LỜI CẢM ƠN Để hoàn thành luận văn này, lời xin bày tỏ lòng biết ơn chân thành sâu sắc tới PGS.TS Nguyễn Tiến Dũng tận tình hướng dẫn dìu dắt tơi suốt q trình nghiên cứu thực luận văn Tôi xin cảm ơn lãnh đạo trường Đại học Nông Lâm – Đại học Thái Nguyên thầy cô giáo trường giảng dạy tạo điều kiện cho học tập thực luận văn Tơi xin bày tỏ lịng biết ơn đến thầy cô giáo khoa Công nghệ sinh học – Công nghệ thực phẩm trường Đại học Nông Lâm truyền dạy kiến thức kỹ cho suốt trình học tập trường, tạo điều kiện thuận lợi để tơi thực tốt luận văn Cuối tơi xin gửi lời cảm ơn tới gia đình, bạn bè, đồng nghiệp tập thể lớp Cơng nghệ sinh học K27, nhóm sinh viên phịng thí nghiệm Sinh học phân tử - Khoa Công nghệ Sinh học Công nghệ thực phẩm, người sát cánh bên tôi, giúp đỡ, động viên góp ý cho tơi suốt q trình học tập hồn thành luận văn Tơi xin chân thành cảm ơn! Thái Nguyên, ngày 08 tháng năm 2022 Học viên Triệu Bích Huệ download by : skknchat@gmail.com iii MỤC LỤC LỜI CAM ĐOAN i LỜI CẢM ƠN ii MỤC LỤC .iii DANH MỤC CÁC KÝ HIỆU, CÁC CHỮ VIẾT TẮT v DANH MỤC BẢNH vi MỞ ĐẦU 1 Đặt vấn đề Mục đích nghiên cứu Ý nghĩa luận văn…………………………………………………………… 3.1 Ý nghĩa khoa học……………………………………………………………… 3.2 Ý nghĩa thực tiễn……………………………………………………….……… CHƯƠNG TỔNG QUAN TÀI LIỆU 1.1 Hoa lúa 1.2 Gen kiểm sốt q trình phát triển hạt phấn 1.3 Gen promoter 1.3.1 Gen 1.3.2 Promoter 1.4 Vai trò yếu tố điều hòa CRE CHƯƠNG VẬT LIỆU VÀ PHƯƠNG PHÁP NGHIÊN CỨU 11 2.1 Đối tượng, phạm vi nghiên cứu 11 2.1.1 Đối tượng nghiên cứu 11 2.1.2 Phạm vi nghiên cứu 11 2.2 Địa điểm thời gian nghiên cứu 11 2.2.1 Địa điểm nghiên cứu 11 2.2.2 Thời gian nghiên cứu 11 2.3 Vật liệu, hóa chất, thiết bị nghiên cứu 11 2.3.1 Vật liệu nghiên cứu 11 2.3.2 Hóa chất 12 download by : skknchat@gmail.com iv 2.3.3 Thiết bị thí nghiệm 12 2.4 Nội dung nghiên cứu 13 2.4.1 Nội dung 1: Phân tích trình tự promoter để xác định yếu tố CRE 13 2.4.2 Nội dung 2: Thiết kế vector chuyển gen 13 2.4.3 Nội dung 3: Chuyển gen vào Arabidopsis 13 2.5 Phương pháp nghiên cứu 13 2.5.1 Nghiên cứu sàng lọc gen chuyên biệt hạt phấn 13 2.5.2 Phương pháp tách chiết DNA tổng số 13 2.5.3 Phương pháp khuyếch đại promoter kỹ thuật PCR 14 2.5.4 Phương pháp tinh sản phẩm PCR 15 2.5.5 Phương pháp thiết kế vector tách dòng promoter 15 2.5.6 Phương pháp biến nạp vector tái tổ hợp vào tế bào khả biến .16 2.5.7 Phương pháp sàng lọc dòng tế bào tái tổ hợp 16 2.5.8 Phương pháp tách chiết plasmid 17 2.5.9 Phương pháp định lượng DNA 18 2.5.10 Phương pháp xác định trình tự 19 2.5.11 Thiết kế vector biểu GUS 19 2.5.12 Phương pháp biến nạp vào vi khuẩn Agrobacterium tumefaciens .21 2.5.13 Phương pháp chuyển gene Arabidopsis .21 2.5.14 Phương pháp đánh giá hoạt động promoter 22 CHƯƠNG KẾT QUẢ NGHIÊN CỨU VÀ THẢO LUẬN .24 3.1 Phân tích trình tự promoter để sàng lọc yếu tố CRE 24 3.2 Biểu gen RMP1 hạt phấn 29 3.3 CRE phổ biến vùng promoter RMP1 30 3.4 Tách dòng thiết kế vector chuyển gen RMP1del ::GFP/GUS 33 3.5 Vai trò CRE với khả biểu gen RMP1 hạt phấn .34 CHƯƠNG KẾT LUẬN VÀ ĐỀ NGHỊ 37 4.1 Kết luận 37 4.2 Kiến nghị 37 TÀI LIỆU THAM KHẢO 38 download by : skknchat@gmail.com v DANH MỤC CÁC KÝ HIỆU, CÁC CHỮ VIẾT TẮT TỪ VIẾT TẮT AS ARN pol bp CaMV CRE DNA MS OD PCR RMP RMPdel RNAi GFP kb TSS download by : skknchat@gmail.com vi DANH MỤC BẢNH Bảng 1.1 Một số yếu tố điều hòa cis quan trọng đáp ứng điều kiện bất lợi 10 Bảng 2.1 Trình tự mồi sử dụng nghiên cứu 12 Bảng 2.2 Thành phần phản ứng PCR 14 Bảng 2.3 Thành phần hóa chất tách chiết plasmid 17 Bảng 3.1 Các yếu tố CRE phổ biến promoter RMP 26 Bảng 3.2 Các yếu tố CRE chuyên biệt mô promoter RMP 27 Bảng 3.3 Danh sách CRE phổ biến promoter RMP1 31 download by : skknchat@gmail.com vii DANH MỤC HÌNH Hình 1.1 Cấu trúc mô hoa lúa Hình 1.2 Cấu trúc gen Hình 2.1 Sơ đồ miêu tả phản ứng BP ghép nối gene vector tách dịng 16 Hình 2.2 Sơ đồ miêu tả phân tử DNA tham gia vào phản ứng LR 20 Hình 2.3 Phương pháp cắt phân đoạn promoter để phân tích CRE 21 Hình 3.1 Tần số xuất yếu tố CRE phổ biến promoter RMP 28 Hình 3.2 Tần số xuất yếu tố CRE phổ biến 28 Hình 3.3 Biểu gen RMP1 quan sinh sản phần mềm RiceEXPro 29 Hình 3.4 Mơ biểu gen RMP1 quan sinh sản phần mềm eFP 29 Hình 3.5 Tần số xuất CRE phổ biến (A) vị trí phân bố Pollen1LETAT52 (B) promoter RMP1 32 Hình 3.6 Kết điện di sản phẩm PCR khuẩn lạc gel agarose 1% để sàng lọc khuẩn lạc tái tổ hợp với vector tách dòng pDONR-RMP1del ::GFP/GUS (A) vector chuyển gen pKG7-RMP1del ::GFP/GUS (B) 34 Hình 3.7 Kết phân tích chuyển gen PCR với cặp mồi GUS-F/GUS-R 35 Hình 3.8 Biểu gen GUS cụm hoa chuyển gen 36 download by : skknchat@gmail.com 45 Phụ lục Trình tự gen RMP vị trí CRE Vùng promoter nằm trước mã ba khởi đầu (ATG), trình tự intron ký hiệu chữ thường, trình tự exon ký hiệu chữ in hoa Vị trí số CRE đánh dấu vùng promoter tương ứng sau: DOFCOREZM AAAG POLLEN1LELAT52 AGAAA GTGANTG10 GTGA SITEIIATCYTC TGGGCY (Y=C/T) ARR1AT NGATT (N=A/C/T/G ) IBOXCORE GATAA WRKY71OS TGAC MYCCONSENSUSAT CANNTG RMP1 (Os01g0533400) ORIGIN -1403 gaacaaaaaaacaatgagaatgacgaccaggattttctataacaatgaatttcattttca -1343 gagcgcagttgcttgctgcaaataatactcttttttatctaattgtatcaccttatctaa -1283 ttttatcacctttggatgtaattatctgagatgatcaacaaagctttaattcctttatct -1223 aaattattgtggaggaatcattggtaaaggaatcaagtcattcattgaagacacacatgc -1163 tggaatcaagatttcaccacaggataacaattttcttggtttgcatgatagactcgttac -1103 tgtcacaggacccttggaccatcagatgcgtgcaatatatttgatatgaagcataagtta -1043 tcggaggacgttcactacccacctaatctgagttccccatttccatatgcaggccttagt -983 ttccctagttaccctggtgttcctgttgggtattatgatcccacaggtgccgtataataa -923 tgctgtgaattatggacctaatgggtatggaggaagggaccaaaacaacaaacccagtac -863 acctatgagatctcacctgctaacaatgacgcccaagattctcactattggtattgctga -803 tgaacattttggtgctgtcgtacgacgtgccggaaggaacattacagagatcattcaggc -743 tagtggtgccaggatcaagatatctgatagggataactggaacatcagaagctattcagg -683 cagcagagtctatgataatgcacagggtgtcagccagttccgagaggtgataaaatctcg -623 tgaagagacgaggggcattatcatctgatggaaaagaattctgatgccaagttaggagga -563 aaaatttgataatttaacgcttttttcattgtccaattactttaattgatatttttgtga -503 ggatgatatgtgagaccatatgagtgtatgaaagtgaaccagaatgtcaaataatcgaaa -443 cccattgaaaatagtgttaaattatcaaatctctcagttaggaggaggggatggagattg -383 tcttttctagttttagcactatccgcaaaaaaaaaagaaacggcacgcaatctcgatttt -323 acggctaaccactaattatcgtatcatacgtctttatcgtatgcatggcgatccggctta -263 actactttgtttgtttctctgtgcaatagtatctccaagattaacaaacccatgcccacg -203 tgtactacgtaattatcctctattgtatctatctccaacgtacggcgcatcgtatcgctg -143 cggccTATAAATtccgtatcacccccacgcccgatctctgcaattcgatcgcttgcatct -83 gcaaagttgcaactcatctcgtctcctcctctcctcccctcctcccctgtagcctagcta -23 attaacttcaaattagcagcagtATGATGGGACGGCGCGGCAGCAGCTGGTGCCGGTGGT +38 GGGTGGCGCTGCTGGTCCTCGCCGTGGCGGCCGATGCCGTGGGCTGCACCAGCGTCAGCT +98 ACGACGACCGCAGCCTCGTCATCGACGGCCAGCGCCGGATCATCCTCTCTGGTTCCATCC download by : skknchat@gmail.com 46 +158 +218 +278 +338 +398 +458 +518 +578 +638 +698 +758 +818 +878 +938 +998 ATTATCCCCGGAGCACTCCCGAGgtaaccgacttgttatactagccgtcaacccgtgcaa cgcacgagataattttaaatattattttttagccctataaaattttactctgatagtatt tcagtcatacaaaatcgtataggaatggtttaaattgatggcaaattaatgccaactgca atatggtgatttttaatttattttctgagtgaattatattttaatcatagaattcgttgt atatttttaaaattgtaagatccatgtattatgaatggtgtacaatattgatattgttat caccgttgagaattatattgaaattgtaactatgcgtagtaatgtgttaagtggaaaact aattaattaaataaattttggcattagatccaatctgtacatgtaaaaagaaataaaaaa agtagccacggaattaatcatatgttggtagcaacataatcattgtccaaaattctgtcc gtgtagtcaacataatcatagactaaactctgtcagcaaagtaattagcacgttactatg tactcttccaggtgctcatttttcaaccgtgtcatatatatagtttacccaggttaaaat tgtttgagatgagaaaccaatctagattgagaaacaataatacccgatcgacttggagat atctgagcgcgtcctaaagttcatctctatttccatttgtgttgatgcctccgcttgaaa tccagatcatcggcaatatatccaactgaattcccaacaaaattgtatgtagagattatt ggatgggatttgtgaggcccatgggaccatgatattaactataaattaataatgtgtggg tctatttgatcaacggcagattcaccaggttaacgctgtataggtatagatattcctagt +1058 +1118 +1178 +1238 +1298 +1358 +1418 +1478 +1538 +1598 +1658 +1718 +1778 +1838 +1898 +1958 +2018 +2078 +2138 +2198 +2258 +2318 +2378 +2438 +2498 +2558 +2618 +2678 +2738 +2798 +2858 +2918 +2978 +3038 +3098 +3158 +3218 +3278 +3338 gtatactatttttgtttttcaatacatgatgtcgttaattttttaatatgtttgaacatt tgtaataatttatggagatacataagatataaatcatgcttaaaatactttgagtgataa aacaactcacaaaaaataaattataattatgtttttttaataagaagaatggttaaatgt gtgaaaaaatcaacaacatcatgtattaaaaaatagagatagttgataatattaatctgc aaattaaatgcatatacaactttgagtgttttgtactagtaggatacagagttgcttgct tgagtgtttcttttctttttgtgccatagatcgtgatgagctaagcagtgcgaggagttt tatgttctatacttttatatacttatgcatgcatattatgttgtgacttatattcgttcg atctttattttcttaatttgtgttaagaaattaatgaacataagcaatatatgtacgtgc gcaaaagattaatttgattttttttctatctaatctaatcttattgttgctatgcagaTG TGGCCGGATCTGATCAAGAAGGCGAAGGAAGGTGGTCTCGATGCCATCGAGACTTACATC TTTTGGAACGGCCATGAGCCGCACCGCCGCCAGgttcaatattactacttttgctttagc acataaacactactacgtactgcagctattacatacaataacaaccatggataaatacta gtaatactataacaatgtttttgctttgatcggcagtACAATTTTGAGGGTAACTACGAT GTCGTCAGGTTCTTCAAGGAGATCCAGAATGCCGGCATGTACGCCATTCTTCGCATTGGC CCGTACATCTGTGGCGAATGGAACTATGGgtatgtatcatgtatataaatgctaaagcat ttgtgtgtcaaagtgataaatagaatagaatattatattgatctgcaaattaatcgaata ttatatctataatgttcatatagaattagagaaaaaaacgaaatagaatagactattata tcgatctgcaaattaatcaaatattatatctatagtgttcatatagaataagaggaaaca agcgagcacactaaactactctagtttctttctataacattattatgtgtccatgcatgc atgttgaagtgctcactgtgttttgttgttttctaaaacaaatgccaaatgtgcagaGGA CTTCCAGCTTGGCTACGCGACATCCCTGGAATGCAGTTTAGGCTGCATAATGAGCCTTTT GAGgttaaataataataataataataaaattctcatctatttttgtgtacttttacatat actagcatataattaatgcaagtgtttttttccatgatatagaATGAGATGGAGACATTC ACCACTCTTATAGTTAACAAGATGAAGGATTCAAAAATGTTTGCTGAACAAGGAGGACCG ATCATCCTTGCTCAGgtattgtaatgatcattcttcaatatgattgaacaatgggaaatt agcatgttaataatttgtactattgtcattccacaatatatattgtcaattaatatcaat gcattaatggatcaatgcacagattGAGAATGAGTATGGAAACATCATGGGGAAATTAAA CAACAACCAGTCTGCATCTGAATACATCCACTGGTGTGCTGACATGGCGAACAAGCAGAA CGTCGGTGTCCCATGGATCATGTGTCAACAAGACGACGATGTGCCGCACAATgtggtgag catgggttttaatcccacaagaatatagaatattatatgaatttatttgtttatcactca tctaataacatgagtttgtcattgttttggaccgctctagGTCAATACCTGTAATGGATT CTATTGCCATGACTGGTTTCCAAATAGGACAGGCATCCCTAAGATTTGGACAGAGAACTG GACTGGCTGgtatttttttttcttccattagtatttctctcattgtttacaaaattaact tttagtacatatatctctttgtttgcaaaattattttttgatgaacatggaggagattta tcttaatttgttgatgtacttaacgttagGTTCAAAGCTTGGGACAAGCCGGATTTCCAT AGGTCAGCTGAAGACATTGCTTTTGCGGTTGCGATGTTCTTCCAAAAGCGTGGATCTCTG CAAAATTACTATATGgtatgctggctagcttgtatctctttcacaatgactgaattttcg ttgatttatgctgagttttttttttccattttttgttagtaccatGGTGGAACCAACTTT GGTCGTACCTCCGGAGGTCCGTACATCACAACAAGCTATGATTATGACGCGCCTTTGGAT download by : skknchat@gmail.com 47 +3398 +3458 +3518 +3578 +3638 +3698 +3758 +3818 +3878 +3938 +3998 +4058 +4118 +4178 +4238 +4298 +4358 +4418 +4478 +4538 +4598 +4658 +4718 +4778 +4838 +4898 +4958 +5018 +5078 +5138 +5198 +5258 +5318 +5378 +5438 +5498 +5558 +5618 +5678 +5738 +5798 +5858 +5918 +5978 +6038 +6098 +6158 +6218 +6278 +6338 +6398 GAGTATGgtaagctttgattaatatattttaccgtactgttaattatatggtgaatttga ataagaaagcaattttttgtcttattttaattataatcttgttggtctacttttgattta ttacagGTAATCTCAGGCAACCCAAATATGGTCACTTGAAGGAACTCCACTCCGTGCTCA AGTCCATGGAGAAAACTCTTGTCCATGGAGAGTATTTTGACACCAACTATGGCGATAACA TCACAgtaatattcagatcacgcccttcttttaatcaaaattatcacgtctaatgcaaac ATATCTAATGCATGCATATTGGTACTATCTCTGATCAATGTAGGTTACCAAGTACACTCT GGACTCCTCATCTGCTTGCTTCATCAACAACAGATTTGATGATAAAGATGTCAATGTGAC CCTCGATGGAGCCACTCACCTCCTGCCTGCATGGTCAGTCAGCATCTTGCCTGACTGCAA GACTGTTGCCTTCAATAGCGCCAAGgtatttttcctttctcaataatcaataatgaaatt aaaatgagaccaagtgtaaggtctataattcatctcttaattttgttttcctcttggtat gacctagattAAGACCCAGACGTCTGTCATGGTGAAGAAACCCAACACGGCGGAGCAGGA GCAGGAGAGCCTAAAATGGTCTTGGATGCCTGAAAACTTGAGTCCTTTCATGACCGATGA GAAGGGCAACTTTAGGAAGAATGAGCTCTTGGAGCAGATCGTCACGTCAACCGATCAAAG TGATTACCTGTGGTACAGAACAAGGTATTTTTTTAAAAAAAAAATCTCTTTATACTAAAG AAAAGAGATCATGATGATGTAAATTTGAAATGATTCCGACAAAAAAATATATATATGTTA CAAATTTAGAACGTCACCAACAAAAAAAAAATTGCAGTCTTAATCACAAAGGAGAAGGGA GCTACAAATTGTACGTGAACACGACTGGCCATGAACTTTATGCCTTTGTGAACGGGAAGC TTATTGgtgagtatcaaagttattttttttccatattttcgttgcaccattcttttaatt ctgtgtgggtcgaaattaaagaatgtttgtgaacctgcgggtcaaaattaaagaatgttc attgacaaacatgtgtgttcataatttgtcagGGAAAAACCATTCTGCCGATGGAGATTT TGTCTTCCAACTAGAGTCGCCGGTGAAGCTCCATGATGGCAAGAACTACATCTCTCTTCT GAGTGCAACCGTTGGCCTCAAGgttcctaattttctttcttgaatgctcacttccatcgt atcagcaacgaactcatcttctttatttgcaattaatcatgtataccagtgataaattgt ttgatcattcttggtaaacagAACTATGGCCCTTCGTTCGAAAAGATGCCCACGGGCATT GTTGGAGGCCCTGTTAAGCTGATAGACAGCAATGGCACCGCAATTGATCTCTCCAACAGC TCCTGGTCTTATAAGgttacacattatttttcatcaatgcttagttgtttgttaactttg ctggtattgatcatatagtgtttcttgctttgatgtttttttgtgttgtagGCTGGCTTG GCTAGTGAGTACAGGCAGATCCATCTTGACAAGCCAGGCTACAAATGGAATGGCAACAAT GGCACCATCCCCATCAACAGGCCTTTTACTTGGTACAAGgtacagtatatgtccattatt tactcactctctccggttttataattcttaacattctgaacaatgacataattttcaaaa tatatttctgactatatttctctattacaatatattaatataaatatatatatatattac ttttgttatagtactttgtaagtcaaatttatatatgttgttttggtgcctttaaactag atatttttaaagtcattattagttaatttataaaagtttaacctaaaacttatcaattat gaaactggagtaatttaattacctgtatgatttcatttcatcgtttcattctttcacctg caaaaataaaaggatttttaatactttttttgttttcttatgaagactacaatttactta atttaccttccctatgaaatttcatcatgtcattaattctttcaccgccaaaacaatctt aatactttggttacttttttttttgttatgaagGCTACATTTGAAGCTCCGTCCGGCGAG GACGCGGTGGTGGTGGACCTGCTGGGCTTGAACAAGGGCGTGGCGTGGGTGAACGGCAAC AACCTCGGCCGCTACTGGCCGTCGTACACGGCGGCGGAGATGGCCGGCTGCCACCGCTGC GACTACCGCGGCGCGTTCCAGGCGGAGGGCGACGGCACCAGGTGCCTCACGGGCTGCGGC GAGCCGTCGCAGCGCTACTACCACGTGCCGCGCTCCTTCCTCGCCGCCGGCGAGCCCAAC ACGCTGCTCCTCTTCGAGGAGGCCGGCGGCGACCCCTCGGGCGTCGCCCTCCGCACCGTG GTGCCCGGTGCCGTGTGCACCTCCGGCGAGGCCGGTGACGCCGTCACGCTGTCGTGCGGC GGCGGCCACGCCGTGTCGAGCGTCGACGTGGCCAGCTTCGGCGTGGGGCGCGGGCGGTGC GGCGGCTACGAGGGCGGGTGCGAGTCCAAGGCGGCGTACGAGGCGTTCACGGCGGCGTGC GTCGGGAAGGAGTCGTGCACGGTGGAGATCACCGGCGCGTTCGCCGGCGCCGGGTGCCTG TCCGGCGTGCTCACCGTCCAGGCCACTTGCTGAttgatctacatgcatatatatggagtg gccatgtctttgattaattaggtctttaattagtcgcttaattaggatggtttgtttatt gagttaagctggtagtcgcccctggtgcatgtgttaattcgggggtaaaaagtgagttta ttaattagggttctctgatcaactgttgattgcagagacgcagagtgtgatatatactgg gaccaaatatttcatctatgaagtacttttacgatcgagtggttaaacatgtgtgttaat +6458 gcccttg download by : skknchat@gmail.com 48 Protein sequence MMGRRGSSWCRWWVALLVLAVAADAVGCTSVSYDDRSLVIDGQRRIILSGSIHYPRSTPE 61 MWPDLIKKAKEGGLDAIETYIFWNGHEPHRRQYNFEGNYDVVRFFKEIQNAGMYAILRIG 121 PYICGEWNYGGLPAWLRDIPGMQFRLHNEPFENEMETFTTLIVNKMKDSKMFAEQGGPII 181 LAQIENEYGNIMGKLNNNQSASEYIHWCADMANKQNVGVPWIMCQQDDDVPHNVVNTCNG 241 FYCHDWFPNRTGIPKIWTENWTGWFKAWDKPDFHRSAEDIAFAVAMFFQKRGSLQNYYMY 301 HGGTNFGRTSGGPYITTSYDYDAPLDEYGNLRQPKYGHLKELHSVLKSMEKTLVHGEYFD 361 TNYGDNITVTKYTLDSSSACFINNRFDDKDVNVTLDGATHLLPAWSVSILPDCKTVAFNS 421 AKIKTQTSVMVKKPNTAEQEQESLKWSWMPENLSPFMTDEKGNFRKNELLEQIVTSTDQS 481 DYLWYRTSLNHKGEGSYKLYVNTTGHELYAFVNGKLIGKNHSADGDFVFQLESPVKLHDG 541 KNYISLLSATVGLKNYGPSFEKMPTGIVGGPVKLIDSNGTAIDLSNSSWSYKAGLASEYR 601 QIHLDKPGYKWNGNNGTIPINRPFTWYKATFEAPSGEDAVVVDLLGLNKGVAWVNGNNLG 661 RYWPSYTAAEMAGCHRCDYRGAFQAEGDGTRCLTGCGEPSQRYYHVPRSFLAAGEPNTLL 721 LFEEAGGDPSGVALRTVVPGAVCTSGEAGDAVTLSCGGGHAVSSVDVASFGVGRGRCGGY 781 EGGCESKAAYEAFTAACVGKESCTVEITGAFAGAGCLSGVLTVQATC Trình tự promoter RMP1del ORIGIN -623 tgaagagacgaggggcattatcatctgatggaaaagaattctgatgccaagttaggagga -563 aaaatttgataatttaacgcttttttcattgtccaattactttaattgatatttttgtga -503 ggatgatatgtgagaccatatgagtgtatgaaagtgaaccagaatgtcaaataatcgaaa -443 cccattgaaaatagtgttaaattatcaaatctctcagttaggaggaggggatggagattg -383 tcttttctagttttagcactatccgcaaaaaaaaaagaaacggcacgcaatctcgatttt -323 acggctaaccactaattatcgtatcatacgtctttatcgtatgcatggcgatccggctta -263 actactttgtttgtttctctgtgcaatagtatctccaagattaacaaacccatgcccacg -203 tgtactacgtaattatcctctattgtatctatctccaacgtacggcgcatcgtatcgctg -143 cggccTATAAATtccgtatcacccccacgcccgatctctgcaattcgatcgcttgcatct -83 gcaaagttgcaactcatctcgtctcctcctctcctcccctcctcccctgtagcctagcta -23 attaacttcaaattagcagcagtATGATGGGACGGCGCGGCAGCAGCTGGTGCCGGTGGT download by : skknchat@gmail.com 49 RMP2 (Os01g0899100) ORIGIN -2056 atccttatccttctccatgaggattctgcgcagagcagcaagtcttctcctccatgtatg -1996 ccttcttttatttatttactctcacaaacccgctccaaatgttcaattgactgattttac -1936 tttactgagagtatgattttcagggccgtgcttacaacgcaggaagaaccacaaggctgc -1876 agggctcttttctaaggtactacttacccttcttgctgactctgttaaaaataaaagaag -1816 ctatatttcttaactacaatttacacaaattaagtaaatattgatgtgaaacctatatga -1756 ttttgctcaatcatgttctattaaaccattgatcagttggttcctagtcttttcagtaaa -1696 aaatgttatgatatctcaactcttcctatgtaatgttatggttacataggctgtgTTTAG -1636 atctaAAGTTTGGATCCAAACTTcagtctttttccatcacatcaacctgtcatgcacacg -1576 taacttttcagtcacatcatctccaatttcaaccaaaatccaaactttgcgctgaactaa -1516 acacagccataatactccctccgtccctaaatgtttgacgccgttaacttttttaaacat -1456 gtttgaccgttcgtcttattcaaaaacttttgtgaaatatataaaattatatgtatacat -1396 aaaagtatatttaacaataaatcaaatgataggaaaagaattaataattacttaaatttt -1336 ttgaataagatgaacggtcaaatttgtttaaaaaaatcaaaggcgtcaaacatttaggga -1276 cggagggagtagtaattgatgcttatgagtccaccaatcaacgatggtacaatagcctgc -1216 cacttgacttgtccaccggtcgtcatggcttgtattgatgtgtcatgatttggattggtc -1156 tgtgcgattaggtgattataattacgatattgtggctatttatgacaggatttttttata -1096 atcttaaacttattataaacagcaagtacagaagcacagggttccattctttccattgca -1036 ttgatttttttttccctttaatgttcatgtcctgctaactgaaagatagtatcttggtcg -976 aatgtaaacaaatatacaatgtccacagagaaagagagattgtgcgcatgacttactaaa -916 ataaatctgattgatgttttccagaatgcatttagcaatagaattgattattctcatagc -856 atagtacttatttttatttattgagatgtttctgtttgtaagcaatattgagaacatata -796 agctaaaccatggagaatagcttccgacgtgcttctgccattcattacaccatgtttctt -736 tctttctttcgaggaccctgcaataaccatgagtctaaaagcatttgtcggcgttcctat -676 tattgtcatataatgattatcaactaagttatgatgtgatacacacacgccatcttcaac -616 ccacaagttattgccatgatggttagatcttcactgaaccaggagagtaacttaattata -556 taggcaaaagatatatattgtggagggaaaacttttagaagcacattttcttaaaaaaga -496 caaattttgatgtgttttttaagactaattttaggtaggttttactcatatgtatatcaa -436 tcatgaaaagagagctaggcaatggaagtggcaaaggaaaacctcaagaaataattttta -376 aaaccaaattctaaatttaaatatcaattgcagcttttaagaaaagttgtgccaagcgga -136 cccaaaaactgtcccttatttgctaccggtaccattacgaacgcccttaacaagccaaca -256 cgtcgttaaagacccaagcgattaaggattcagtaagctagagcaaaaatatgaaaaaag -196 aagttactatatctctgtctaTTATTTtctgatctgtgcaatcaactttacgaacagaaa -136 agttagagcaaaagaaaaaaatctagcatttcatcactcattaccctacatgttttaact -76 atgttccattttcgttaccttttgcagggtgccttcaggggaacgtgaactcaaattatt -16 aaggcctggtaacacaATGGAAGTGTCTATGGCAACCACAAACACTGCAGGGGAACCAGg download by : skknchat@gmail.com 50 RMP3 (Os03g0381000) ORIGIN -2064 tcttctagttcccaatctctcctccctctttttttctgcgcacgtttcccaaactgctaa -2004 acgatatattttttgcaaaatttttctataggaaagttgctttaaaaatcatactaaccc -1944 atttttaaggaggccgatataaattggacgtccgatctattgcaaaagatcagacaacca -1884 cgtctgcatgacatcatcaagcatgcatgcattcaaaactattttaaactatttttctct -1824 taaattatttatccaaattatgatccgattgcaccattaaattcgttgcaattaaatctt -1764 taaaacaagaccacacatggatatattccgatgaaaaaaattagcttattattgaattat -1704 ttttaaatgttgcacgctgttccaccaagtatatcagaattgtttcaataggtagatcga -1644 aaatatttcaatcgtttaaatcggatgatgtttcacattatataaaacaatgtttcagca -1584 aatagcgaaagaatgtttcaagTCACTGCAACATTtgatccatacaaAGTGAAACATTAT -1524 aggctggtttggtttgagacctaaattaggcttaccaatatttggcatTTTCAATAGTGt -1464 ttagtgtctagttggtttgaagccaaatttttgcatgcctaagaaaataggccaTTTCAA -1404 TAGTGaacttaggctattttgtcttcaatccaaatacaacattgcCTTACCAAAATTagt -1344 catgccaaaaCTTACCAAAATTtagcactgacaaaatttggtaaggcctatttaggccac -1284 aaaccaaaccaaccctatatgtatactaggtgaaatatttttggtaaaacaataaatgaa -1224 acgaattctcttgatagagggtttccataatatgtgagtgatttattgcaaaaaatgttt -1164 taatTCACTGCAACATTagatctatacatAGTGAAACATTATgagtgcactatgtgaaac -1104 atttttagtggaacaaaaaaatgaaacggatttccttaatagagggttaccaaaatatat -1044 gggtgatttgttgcaacagaatatgtggtggggacacgtggcagcacgaaggcgcgtggg -984 agagacgtgggcagcacagcaggcggcgtggtgggccccggcgacagcagatgcctggcg -924 ctaagcattcccgtatttttaaagtttttttagctaatacttaattaatcgtgcattaat -864 ccatcacccgttttccatgtgggaggtgagggttccaacctaagaacatagccttaggct -804 gtgtgtttagttccctttaaacttctaaaaatttcgtcacatcaaatgtttgacatataa -744 atggcattaaatgtggacgaaaaaaaaccaattgcatagtttgcatgtaaattacgaaac -684 gaatcttttgagcttaagtacgctatgatttaacaatgtggtgctacagtaaacatttac -624 taatgccggattaattaggcttaataggttcgtctcacagtttacagatagaatctataa -564 ttttttttcgttactagtctaTATTTAAtactttaatttttgtttgaaaagtctatgtct -504 tggatggcagcggatacatcacggttcacggggattcccttccccctcgagcgacggcgc -444 gagagagattcaggttcgggttaacttttccggtcccccgctgggttaccgggtacccag -384 gaacgccgcgtgcaggcctccggtgcaatggcccaatgggccaaacgggtacggaggtgt -324 ggcttggcacttggcaggtgcTTTGACCGGTCAAAtgaggtggtttagatttaactctta -264 cggttaaggtgttctgttaactaatctgaacaacatgatgctcatcctgtaagcaggaag -204 cctacgtggcaactttacggcgcaacggccccacatgtcagtgggtgagaaacccgcgct -144 agtagatcctgttgactagaacgatagtcatcaactcatcattctcgagacctccaccta -84 taaataggcaaccgagatttacacagcattagttgcacggattagaggaagattagtagt -24 attagtagtcgggattaggcagtaATGGCGGCGACGAGCGCCAGGAGCGTGGCGTGGCAA download by : skknchat@gmail.com 51 RMP4 (Os04g0561900) ORIGIN -883 gaggctttgccgcgagagcgagtgacgcgcgtgctttgtagcccgtgggctgcttttgcg -823 gtgcagctccggcccatttggacgatctcatggtgaagtgcccaagagttttaccagttt -763 ttctttcaagtcaaaaatactagctatgatagtccgcgttgctggcaaatacggattcaa -703 aatttgccttgtaaatttaccagttgatcggtggctacaactgtcttgtattttttttta -643 accttgccttccaagttgagatcggtggtaatactgccttgtaatgcagtcaacaatgag -583 cacaaacgtacgtgttcctttcctttacgcatacatatgagtacacgcgcggtctgtaaa -523 ttaatcacaactttgtcacaaaacttattccggaccagtttaatttaggtcgtatcaatg -463 ctggacatgtagaagagatatccataaaactgttccgcgttgaaaatataaggacatgaa -403 caaaaccggacaaacaaagggcagataagacattggaacttggaagttcgtctatcccca -343 aaggcatcagctaagatgaacagaaacaccacgaattttacgaccgtccatcgctccata -283 gacataccacaaattctgttaccaatccccttgtaatatcttatctcttttgtaacagaa -223 ttaaaattcctcctaaactaaaccaccctgcctaaactcaataacgatgatttttttcac -163 gtacatcgatgctgttgcggttgcgtcccagtagatctcatctataaaatgtgcgacgta -103 cgggacccggagcagagtccacagcaggcgccgcgcgcgctatcgatctccgccgcctga -43 ccgccgtaccaccgtctcccggcagctagcgtcgaccggcgtcATGTCGTCACGGCGAAG download by : skknchat@gmail.com 52 RMP5 (Os04g0650200) ORIGIN -2064 gtaattagacttaaaagattcgtctcgcaatttacatgcaaactgtgtaattgatttttt -2024 tcgtccacatctaatactccatgtatgtgtataaacatttgatgtgacggaatttttaga -1964 aatttaaagggaactaaacacaacctctatcgtctttccgcccacagagaaacatcttct -1904 actcgtcaatttaacacttcgttccttccttgccgttggaaactaatttttctcgcacgc -1844 aaaacggagcaactcattaacataactaagtattagTTTTTTTTAAAAAAAAtagattaa -1784 tatgattttttaaaagcaacttttgtttttttaaaaaataaacaaaccatttaacagttt -1724 aagaacgtgcgtgtggaaaatgagaagaataagttataaacttaaaaaaataatgcagtt -1664 cttggattctaggcatctatcataggacgataaaaaattcaagagtaaagtgtacgggca -1604 gtctttaaacttgtaggggtatgtcttctagatccctaaactctcaaaatgcatatccaa -1544 gtcccataacttgtcatagtgtgccatctaggtcccaaatcactaaaacctcttcaggtt -1484 cttacgtgacgttgatgtgtatgcctcacggacatgatatagaattattttgactgacaa -1424 atgggacccatttaattttttttcctttttccttttcttttatctttttcccctttcttc -1364 tcctttctctgtgacccgtgcagaaaaaaggaaaaaaataagaatgagaagaaaacgaaa -1304 aagaaaaggaaaaagaagaaaaaaaaggaaaaggacacgtcacgtccatgtggcattgcc -1244 atatcagcgccacataggatcttagaggggatgtgctaatttgggacctagatgatgcac -1184 tttgacaagttatgcgatccggatatacattttgagagtttatagacctatatgacacaa -1124 ccctacaagtttaagtactgctaatgcactttactcaaaattcaatatcatgctgttcac -1064 ggttaagagagaaacaaaacgagcatcgtagtacgtaaacttgtttgaaaaaaagttttg -1004 taactgcgaacagaattacagaagactacagtaatcagtcagatggaaaaggtatacctg -944 aggttcctctcatcatgtcacgagatacaaaatcgtgcttttcaaccgtaaaactggata -884 aaacgtatcccccaacttacaaaactagtacagagtatgatctttgaggttttgctattt -824 cacttgcgatagaattttgagttcttatactctgacttttcacccgtacgatcttgtatc -764 aagatttgtgttcctatgaattcaacttgcaaagttgcaattacatttagttacaaagta -704 cttatatttggttatagagtatttacacttaattacagagcaactacactcttacattaa -644 aaaaacatattttacaacagcttctttctcaagatttatgattttctatatgtttgtccc -584 ttttcttttatttcattcaaaaatggctatatgtattggttggaagttctgtaaaATTTG -524 AAGCTTCAAATctattgaaatatcaatgggtagtctcgtatttttattggcatatataga -464 gataaaaagtatataaagtttagtttagtgtaaaactcctgacaattagattttcttata -404 atatataagcaggaagataagattgaactgctttatcacatggataagcgtaggagctga -344 gcggccggcgtgattcggcccacatgattgggctggtcgtgtgggagcattttggccggc -284 cctggtagataggagtacgcgatcacattggaaaatcacagctgctcgctgcacgtcgtt -224 ggagtacttcctaatcccaactgttactcggaagttaagggcgccagcgccgcgtgatcc -164 aatcacctcgcaaaatcaccttgagaatagtatattcggaacagatttgcagcctcgtcg -104 tgtcTATAAATagggtggtaaacacccatgcatgcaaagccacaagcaattcaacgagtt -44 acaccttccaccgtcaaccgaggagctttcagttgttgccggccATGGCGGCGAAGCTCT download by : skknchat@gmail.com 53 RMP6 (Os06g0681100) ORIGIN -2193 ctaactttttggattgttgaagaattataataaaatgctattgcttctttgactatttgc -2133 ttgattggttattgatccttactttataagttgtcctcatatattcttggtaagttatga -2073 agagtttagctaccaatgtaatgaaatgtaaataggttagaattcttgcaactagccaac -2013 tattgctttgaagtattattttactaactttttggattgttgaagaattataataaaatg -1953 ctattgcttctttgactatttgcttgattggttattgatccttactttataagttgtcct -1893 catatattcttggtaagttataagaaagcaaagttttgtacatacctccactcttaataa -1833 aatttggtcaatctttctttggctaatccggcaaaaagaaaagaaaagaaaaaagaaaca -1773 aaaagaaagcaaagttcctgtcgttgaattataagaactgtagttcgcttggagctgaag -1713 tgtgatctatcactaaaatgtcgatatgaaaataataaaatgaaaaggattaaattcggg -1653 gccctcattacaccttgtatttctccttcgtgttttctaataatgaagatttgtttttta -1593 aactcatatatataaattctaaagggataagtctatttgacccccctgaactattgcgtt -1533 cgtctaaaatacaccccttcacttcaaaatcagatatctatcccccttaactttcaaaac -1473 cattcaaattacccccctgtCTTCAATTGAAGtggttttaaacagatctttaatccagca -1413 catcattttgtttcactaagatgtgtgagatatcatggtatagattgaaactgagaggag -1353 tggtggctggtatatcggactcaaaaggagtggtgccttttatttttcgtttttaaggGG -1293 GAATCGGATctatagttgaacttttttttcgtataagagaGGGAATCGGATagaagtcgg -1233 actttttttttaatttttttcgctttttttttctttcgcccggtttttttgaccagacaa -1173 ttattgttggggaatcagacaaattcatttttcccctgatttttcttttaccgcatcaga -1113 tttggtttttcttttcttttttcgcacgttttcttctgcccgttttttttggatcggact -1053 attttggttttttggcccggtcttttttcgccccttttttctagataggacagatttgca -993 gatttggtttttcttttttttcgcacgttctctttcgccctgttttttttgaaagaacca -933 cggtaggggaagcctccaccgttaagattttaTATTAATaaaggggaaaaaattacaata -873 aatttaaccactgtaacaatgtatttctctcagaatttttgactctatgaactaaaaggg -813 agagatcattcctaaagagaactctccaagagggaacaatgttttcaaaaattaaactat -753 tcctctgcttccaaatgttccaagcagcataaagcaccttctcaacaaaatgggtagagt -693 tatatgaatttctggccgtgaccaacatttcaccaaagcttaaagaaagattccaaacca -633 cactaaattacgcccaacattgagtgctaaaaggacactggaaaaataagtggagagttg -573 tttcccttgatcctgttgaacaaagtacacagagcaaggaagcattttgaggggagcaac -513 gccctattttttttggatcggataattttggtttttttttcgctggtttttttcgccttt -453 tttttctggatcggacatatttggttttttttcgtactttttttcgcccggtttttttat -393 tggataaatttagtttttttcgtccgttttttttacggacgatggaatttaagtagtaga -333 gataaagataaaagaggctactgtataacaacttctccgtgcttatcacctcgtccagtc -273 cagatagaaacctcaacaaactggccctagctattcgctcccgcagcattcgaactcttt -213 aaatcggaagagcccctcacccctcagctttcacccgcgccgccttttccaccaccaaac -153 ccttcgagtccccgtcgcagttgcggcattccgacgaacacctcgcgcgcgcgcgcgtcg -93 ccgcctccatctcctgcgtcgccgcggcgtctcacaccaacacctcgctcgctcgcgtcg -33 cgtcgcgtcgcttgatctcgcaccgggggggagATGGGCAGGGGCAGAGGGAAACAGCGG download by : skknchat@gmail.com 54 RMP7 (Os07g0664600) ORIGIN -2066 aaaattatcctttgagtctctctccatcttattcctgtattttttctgcagtccaatcaa -2006 actgccatttatatgtcttttctatgttttacaattctctgttttttacacattcttatc -1946 acaatcctgcattttctctataggcctgtttggttagtaccacattttgccacacttgtg -1886 gtatgttagtttgaccaaatgtggctagtgtttggtttgtttctataactttggcatgcc -1826 acactttctactcttttggtcccactagtcatagacccttttcttagccaaagtttgcca -1766 caagtgtggccatcaaattttgagccacacaatttgcatcttgccacacctttggtataa -1706 aaactgtggtaaattaaggtgggtatccaaacagtccatatgtcttcgtttaagtcattt -1646 tactagggttgttggtctgttccatccagagtggttggatgtgtacttcttgctatttca -1586 acaggtactacggctgtgtttagatccaAAGTTTGGATCCAAACTTcagtcctttttcat -1526 cacatcaacctgtcatacactcacaacttttcagtcacatcatctccaatttcaaccaaa -1466 atccaaacTTTGGATCCAActaaacacatccgtaacattaaaacttggcataaatacaaa -1406 tcatttcggatgcttttcttctcatcagacgcagagcctagaacagaacactgataatct -1346 gatactatcaatgagcatggctatatcaatgaatatgacgaAGAGTTCTATCgtcgatgc -1286 agtcttcaaatgcaagtttgatcagttatgcctatcgctttaatccccggttgtacttca -1226 tgacagAGAGTTCTATCctcgatccacactcttcagcttttaaaatgacaactttggtct -1166 ttgggtactaaaggggtccatcgattcttcctcaaaggtagatgaaatcgttctccgaca -1106 actatacatgcaagtgacatattataaacgaattgtatttctcgttgaaacgcacgggca -1046 cattactagtaagttataatttgtgaagcattttgagatgttttgtcatcttctgcgaag -986 ggcaatatgatatccaaaaggaagttctgagaaccttggactactattgtggtttttatt -926 tttctggacattattgtccaacagttggtcccctcatgtttaaaatgtggctccaccaac -866 gcacatgaaaggcgccgagtgctctcatcgcaggtagggccacaagcgctctcgtaaggg -806 cccgtgcatgtccaatgtggagcctaatgtccaagctgatctttctaagaccacttcatc -746 gcattgtgcatgctccaatggtacttctagattgataatatttgtcgttttagataaact -686 tgatgctaaacttaaaaatatttgactaatctctaatatctttttgtcttagaaatatgt -626 caaatgtataaattaatcttaaaaaatatttcaataaattcatatatttgttgacgtttc -566 tatacatattataattaaaaatattggttaaagttatttttttaagaccgtatccttatc -506 taaaacgataagaccaaccctcctgtacttcaaaaatttacgcaacacaaaacccactaa -446 ttattgtccttcatttggcccttcatatatgctctctccgtaaaaaaaaaaaaccttgtg -386 tccatgttcaaatttaggagtgggttttttttaagaacccgtaatataaaggattttgag -326 tttttgtttgcattttttgaccactcgtcttattcaaaaaatttgtgcaaatataaaaaa -266 tgaaaagttgtgcttaaagtattttggataataaagtaagtcaaaaaaataaataataat -206 ttcaaaattttttatgtaagacgagtggtcaaacagtgtaagaaaaaactcaaaatccct -146 tatattatgggatggagagagtaccattttcccccagttttttcaaacTATTTAActagc -86 ccctctctactgtgacctttggactcgagcttggaatcgaagaaccagtgaacacgagag -26 cacgacgccggtggcactcgttgaggATGATCAACGCAGCTGGGCAACTTCTTCTCAGgt download by : skknchat@gmail.com 55 RMP8 (Os12g0605900) ORIGIN -1422 tactgctgtcacatacagtgacaaaaacaattaaacctcatttgttcttccattgccctg -1362 aaagttggaataaccgtgaaaattggaataatcccagtaatgaacgaactaacctgacac -1302 tgacagtgaaaactaaagcaactgaggtcttgtttggcatagctccgggatcaAAGATTT -1242 TTTTGGagctgagtatttctagctacacaacaaatccatggatcatgataatatttggac -1182 gaattattttattcatatttttaataaaatataaaatttgcaagcactatattttaactt -1122 acaaacttcagatctaggatttgggatcttggagttgtgaatttgtgataaacataacct -1062 gaatttgtgtaaaacggggccgcaagtagtcttgttccagcccatgtgaaacagaccgta -1002 cataggggtcgtaataacaagtacaaccttttgacatacaaaatctaacggcgggttaaa -942 aatgagatgtgctaaaatttagtagattttttagggcttgttcactttgatgctattttt -882 aaccttaccaaatttgggtaaagttgccaaaaaaagtggctatatttagtttgctgccaa -822 attttggtactatataagaaattctgccaaTGCCAAAATTTTGGCAactatgccaaaatt -762 ttggtaatgccaaaatttggtAAGATTTTTTTGGcatcaaagtgaataggcccttagcta -702 ataaaaatacaattttgctatatATTTGTCGACAAATtttccaccaaaaatttgacagat -642 gtcgtAGTGTAATTACACTgtaactataatgtaacttgtacgtaactttcaaaaatatct -582 ccgtaacatgttatttcggtaaaatagaggttgtgggaacaaattctttcacatatacgt -522 gtgttgtgattgttttttcttcctcaccaaaataaatcctgtaatagatctaacgattca -462 aaattacatgaaacttacatataagttacactgtagttacatgCAAATTACAGTGTAATT -402 ACAtacaaattacAGTGTAATTACACTacaattgtactataattacatttgtcaaatttt -342 taggagaaaATTTGTCGACAAATatataggtggtcacataaaaaTATTTAAgagctgaga -282 tgggttgtaaaAGGGCCCATGGGCCCTggcccattaccaccacccatgcttgccgggctt -222 ccggcccacctacgtaagccccaccacgccgccaccgccaccaccgcaaccgtctccgct -162 tcctcctcctcctccccgtcgtgttcgcgcgcgacttccgtgacccacctccgccgcctc -102 tcgactcctccactctatatctcgagccctagaaaaaccctagctccagccactatctcc -62 actagcctacgtccaaggtcgtcttcgtcgtcgtctccgtctcgagcggccgggcgcgat -2 cgATGGCGTCGAGCTGCACCATGGTGCCGGCGGATTTGGTGGAGGAGAAGGCGGCGGCGG download by : skknchat@gmail.com 56 RMP9 (Os12g0637100) ORIGIN -1450 ccttagctagtagtgagctactagtactagttgccatcgagaggtcatcttctccttatc -1390 tttctcttcttcttctcctctcaattaagatacatgcatcatgtgaaagcctcatgataa -1330 taccaggtcaagcagggcaacaactttatttctgttgattacaagttagtacaatgcgaa -1270 ctaaagaaaatttaggcattacttattcgagtcaaagcttgcccgtccacgacgccgata -1210 gccagtgagcttgacacgcgacctgccattgccactgccaaattttatggcattattcag -1150 aaaacttatactatatgctagttgattcagggaatgaaagtagctagactgctcctaatt -1090 cagagttcagaaagttaaGATGAATGGGTGGTcggttttcaaaaaaaaaaaaaGATGAAT -1030 GGGTGGTagcttattcagatttcacaaaagggaagagcaattgtggtattttaactcgga -970 tcaaccctctgaatgatgctgtcagttactggcactaacggttttacacagaaattaaac -910 aaaagtagaatctataaaggtgaatctgctgtctagtgcagcacaTAAAACGATATgagg -850 caatcattttgggggcaaatctgtgaacaagtggttaccaaatcgaccaaaaggtggcag -790 catgcttctctgtggcatgcttttggctagatcaagttgtgaaattgcgatctcctagct -730 agctttttcttcaaggccaccactgtgtttcaaagatgtagcgagtgatacatggatggg -670 caagtaacagtatgtgcattatactgaattctactagtaaaaaagtagagagctaagttt -610 cctttgttgaagaggtctgaatgatatgatatctttaacatatcttaacggaacaaatat -550 aaattgtgttatgcttaatatcatctggaatatactggcatatgataagcagttcacatt -490 acatcattTAAAACGATATccacgggtgatgtttcgacaaagaagaactgcaagcctgca -430 atgccagcacacatgtcgcaatcttttgtgaattggtggtgtgttttgtcagtcaaagta -370 ggcgcccatacaaatcagtagaaggcaaggcaaagcgatgtgagatttgtttgtccctcc -310 cttgcttggttggttgtacatgctgcgaatcgaagaataaatcagtagggatagaagagt -250 acttgtgttcaaagtcaacttgcaagtccttaatctccaagattgatccgcatctccaac -190 taaaccctctcctaatctaatcacggtctaatggcaccatctatggatattccccacaaa -130 ccattcagattagttcaagtcaacattaagatgcacgcaacactctccTATTTAAgagat -70 gtatcctttcttgcttgtccaacgaaacagagtagcacaagaagcagagtgcggctagct -10 cacagtcaccATGGGGATGCTGCGGTGGGGAGCTCACCTCCTCCTCCTCCTCCTCGCCGC download by : skknchat@gmail.com 57 RMP10 (Os12g0637900) ORIGIN -2100 tctttaatccctaatatatgcagattaatggttgagatctgaaccctgtcaatgtgctac -2040 tagcatggtaatagtatagtagcttttcaagtaactaggtactcaaggatcaagatcaag -1980 catgcgAAATCTAATCTTTCAGAATCAGAGCAATGTTAaaatccAATCTTTCAGAAccag -1920 agcaatgtgaaatcgctcagatagaagctaataagaaaaaaaaaggaaaagaaaaatgtg -1860 cattgtcattgccattttgccatgacattatggtggagccacccagtacatgtcataccg -1800 tgtttagtaaagcgctaataaagcctttcaaaaggctggcctgaagtgacaaatgcttcc -1740 agacactggtatggtagcaaaacacaacacatgcatacttttacatgcatctatcagctg -1680 ctgatgctgcatgctactacagcactgcactgatcatcactagtggatggtagtagtgag -1620 agaacactgtaatggcatgaattttttcttctttttttatcaaaggaatggagggacaac -1560 gctctccatttctattagagaaaaaacaattttacgattcttgagaaggtatcatttttt -1500 ctattctaaatttaatacctattgatatctaggtactataagataacaaattttacatta -1440 aatttttgataccttctcaagtactgtaaaattacttttatagaaatggtaagcttgtac -1380 ggtacaaaagagtctttgcctgagacaagtccatggtagcacacacccttgaggctagtt -1320 atggtagagacgagagatggggacatggggtacatgtacagtaataatattgcacagaga -1260 gagagagagagagagagagagagagggcatgcatactccattttactcccacgagttaaa -1200 attgttggagtggtccacatgtcgatggcctttgctgtgttgacagcctggtaactggaa -1140 aaagggtagtacagtaagcaggcagagtaactgtagttcagattttgggacccacaatct -1080 gaattgtgaagcgaggcatgagacacgcgtgaaagaatgattggacggaccacatactaa -1020 ttgtcactagctagggtgtgttattcttcacttcttagtggagtactatgaaatactcat -960 aacttctatcgtcggtttattgtattaggagggatcacatccccttctagaatacatttt -900 ctttaggcggagggtttagaactttaaattagaaagcaattcatacaggcatgatgagTA -840 TTAAttccacataaaaaaatcaagggacagaattgatactactactaacttctcgttcaa -780 taaaagaacaacttttagaagcgattgtaggacgagtttgtataagcatttgtttagatt -720 tgtctgttaataaagttgatttttttttcgctaaagcacgattaaaagagctataaggaa -560 tcaggccaaccatgcactgacctgaccttggtaaagcactcttgcagttgcccttgctgt -500 cgtgccggaggcgtcgatagttggtcgccggcgtgatctaagcctaatagggttagagtc -440 aaactgagttgtgatgtgtttgtgttggactggttcgtcatgggctggacgtatgtgcgc -380 tagctttggtgggcctgcagccgaccaaggcccggcccactatgtacgttgtgtttcaac -320 tgcctggtactactagtatagataggtgtacatttactgcaaccgcgagatcagcggagt -260 tcgtttctcaatgtcgatcatctaatctcatcgatggagataagaagatgaaattcagcc -200 cttccttcaccgaaaagaaagaaaccaatcatataatatactgtatcttcgtcgttaatt -140 aattcgttatctccatatctctctctctatatatatactcgcagccctatgctctgcccg -80 cgcactgtaaacaaattactaatagattctccgacgatcgtagttgattgttcgtcaccg -20 agatccgacaaacgccatccATGGGTCGCGGCGGCCGCGGTGGAGgttagattcttcgat +41 cgaccttaattttgttcttcgatcgatcagtcgtgcattatttggtagatccggccgacc download by : skknchat@gmail.com 58 Phụ lục Kết so sánh trình tự promoter RMP1del ngân hàng gen NCBI >dbj|AP014957.1| complete sequence Length=43270923 Score = Identities = 570/573 (99%), Gaps = 3/573 (1%) Strand=Plus/Minus Query Sbjct 19302622 Query 63 Sbjct 19302562 Query 123 Sbjct 19302502 Query 183 Sbjct 19302442 Query 243 Sbjct 19302382 Query 303 Sbjct 19302322 Query 363 Sbjct 19302262 Query 423 Sbjct 19302202 Query 483 Sbjct 19302142 Query 543 Sbjct 19302082 download by : skknchat@gmail.com 1038 bits (562), Expec ... trị yếu tố điều hòa hoạt động (CRE) vùng promoter chuyên biệt hạt phấn lúa? ?? Kết nghiên cứu khơng xác định vai trị yếu tố CRE mà sở để tối ưu hóa kích thước promoter, tạo nguồn vật liệu tốt cho... tiễn Phân tích vai trị yếu tố điều hòa hoạt động (CRE) vùng promoter chuyên biệt hạt phấn lúa (Oryza sativa L) góp phần nâng cao hiệu công tác bảo tồn chọn tạo giống lúa có phẩm chất gạo tốt,... khắc phục điều nhà khoa học có xu hướng sử dụng promoter chuyên biệt để điều khiển gen mô, phận theo chủ đích [3] 1.4 Vai trị yếu tố điều hòa CRE Yếu tố điều hòa cis định vị vùng điều hòa promoter,