... biological/physiological and engineering perspectives Proficient skills in handling cells and chip In our case, we need to integrate cell culture, ATP release measurement and Ca2+ imaging into the one system The main ... identifying the structure of the mechano-sensor in VECs membrane, finding signaling pathway given certain type of mechanical stimulus and investigating the interplay of gene expression and cell ... adjusting shear stress and ATP To achieve the ultimate goal, we break the whole problem into subsections experimentation, modeling and control of calcium dynamics and tackle them one by one In Chapter...