Báo cáo y học: " Changes in microRNA expression profiles in HIV-1-transfected human cells" pptx
Báo cáo y học: " Altered retinal microRNA expression profile in a mouse model of retinitis pigmentosa" docx
... modified miR expression profiles in reti-nal disease.Results MicroRNA expression profile in wild-type retinaRetinal miR expression was initially evaluated using micro-array analyses. Comparison ... parts of the eye, includ-ing the lens, cornea, and retina [26,27]. Most recently, usingmicroarray technology, 78 miRs were found to be expressed in retina, including 12 miRs, whose expression ... upregulated, expression of their targets may possibly be suppressed in P347S mice. Many genes encoding factors that are involved in mRNA processing and splicing, and RNA-binding proteinsbelong...
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Báo cáo y học: "Silencing CD36 gene expression results in the inhibition of latent-TGF-β1 activation and suppression of silica-induced lung fibrosis in the rat" pdf
... 2B).Lv-shCD36 could reduce hydroxyproline content in lungHydroxyproline content is an important indicator of lungfibrosis. In this study, no significant differences in thehydroxyproline content of the ... analysis at 570 nm using a micro-plate reader (Bio-Rad 550) [14].Determination of hydroxyproline contentThe lung samples were measured for hydroxyproline con-tent using a hydroxyproline kit ... trachea.Either physiological saline, silica in physiological saline,or silica with Lv-shCD36 or Lv-shCD36-NC in physiolog-ical saline, was instilled into the lungs using a 14-gaugeneedle inserted into...
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Báo cáo y học: "MHC class I expression protects rat colon carcinoma cells from hepatic natural killer cell-mediated apoptosis and cytolysis, by blocking the perforin/granzyme pathway" ppt
... JC: Human and murine cytotoxic T lym-phocyte serine proteases: subsite mapping with peptidethioester substrates and inhibition of enzyme activity and cy-tolysis by isocoumarins. Biochemistry ... cells was completely inhibited (Figs. 2, 3).The results obtained by granzyme inhibition and Ca2+chelation strongly suggest the involvement of the per-forin/granzyme pathway in the anti-MHC I ... independent in the perforin/granzyme path-way [31–33]. As a consequence, this caspase independentcytolysis induction can be used to characterise the per-forin/granzyme pathway. Indeed, when...
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Báo cáo y học: "integrating phenotypic and expression profiles to map arsenic-response" potx
... One such interesting proteinis Cad1 (Yap2). Although Yap1 and Cad1 are nearly identical in their DNA-binding domains, Yap1 controls a set of genes(including Ycf1) involved in detoxifying the ... SOD1ATR1CAD1GTT2YLR108CAHP1LYS7YAP1PHD1MSN4YGR010WBUD20SRB6ADO1 GDH3 YER079W YHB1ZWF1 YNL087W YDL124WYLL059C YDR061W YLL055WYDR533C ERG28 AAD6 YGL114WSEC9 YGR011W YJL048C RPL10YLR460C ERO1 YMR251W TRF4YDR132CLSB6 ... 4.8 NC O-Acetylhomoserine sulfhydrylaseSTR4/CYS4 YGR155W 2.0-2.7 2.5 -2.0-3.0 Cystathionine B-synthaseSTR1/CYS3 (4 h, 100 µM AsIII) YAL012W -3.5 13.4 NC Cystathionine-lyaseSTR3 YGL184C 2.2-3.9...
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Báo cáo y học: " Non-imprinted allele-specific DNA methylation on human autosomes" pptx
... methylated18 cases: G hyper- methylated in right part of ampliconAlways: G hyper- methylatedAlways: del hyper- methylatedAlways: C hyper- methylated1 case: A hyper- methylated in left part ... hypomethylated. In the remaining heter-ozygous individuals, both alleles were low methylated (Table1; Figure 4; Additional file 7). The gene expression analysis in leukocytes from individual ... variation of DNA methylationA summary of the methylation of all amplicons in differentindividuals is shown in Figure 1a. The methylation levels ofhighly methylated or completely unmethylated ampliconswere...
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Báo cáo y học: "Hematopoietic Pyk2 regulates migration of differentiated HL-60 cells" pptx
... differentiate into neutrophil-like cells (dHL60) by incubation in medium containing 1.25% DMSO for up to 4 days. Pyk2 expression and tyrosine phosphorylation was measured by Western blot analysis. ... signalingpathway [19], although the functional activation of integ-rin was not determined in that study. Our results indicatethat Pyk2 is not involved in fMLP induced inside-out sig-naling ... adhesion kinase family and has been implicated in neutrophil spreading and respiratory burst activity caused by TNF-α. However, the role of Pyk2 in neutrophil migration is incompletely defined. In...
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Báo cáo y học: " Changes in microRNA expression profiles in HIV-1-transfected human cells" pptx
... non-specific generalizedcellular toxicity. Interestingly, in our assays, miRNAsupregulated by transfected pNL4-3 were exceedingly rare.Pending further understanding of mechanisms, it is con-ceivable ... response in HeLa cells to pNL4-3 transfec-tion is the downregulated expression of many miRNAs.Studies are ongoing to examine changes in miRNA expres-sion patterns in human cells (primary and ... of RNA silencing encoded byplant viruses and their role in viral infections. FEBS Lett 2005,579:5958-5964.10. Ye K, Malinina L, Patel DJ: Recognition of small interfering RNAby a viral suppressor...
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Báo cáo y học: "Changes in the expression of NO synthase isoforms after ozone: the effects of allergen exposure" doc
... form of the enzyme(iNOS) is involved in inflammatory disease of the airwaysand in host defense against infection [2,3].We previously demonstrated that NO metabolites wereincreased in tracheo-bronchial ... pulmo-nary inflammation or lung damage [22]. Chiba et al. [23]reported that NOS activity in airway tissues was elevated in antigen-induced AHR rats, mainly due to the inductionof iNOS in the airways. ... Itis synthesized from L-arginine by three forms of theenzyme NO synthase: two constitutive NO synthases(cNOS) are involved in the physiological regulation of air-way function, and an inducible...
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Báo cáo y học: "Changes in gene expression during the development of mammary tumors in MMTV-Wnt-1 transgenic mice" ppt
... development.</p>AbstractBackground: In human breast cancer normal mammary cells typically develop into hyperplasia,ductal carcinoma in situ, invasive cancer, and metastasis. The changes in gene expression associatedwith ... in this study werearrayed at the National Cancer Institute microarray facility.All slides of each array type were printed in a single batch. The8.7k slides contain the 8700 Incyte GEM1 clone ... Biology 2005, 6:R84orescent images of hybridized microarrays were obtained byusing a GenePix 4000 scanner (Axon Instruments, FosterCity, CA, USA). Microarray images were analyzed using theArraySuite...
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Báo cáo y học: "Changes in protein expression during honey bee larval development" doc
... [26,27].Proteins with high copy number, including the many dis-cussed above, are always the first to be investigated in anyorganism. The difficulties in studying proteins in honey beelarva ... consistently found only in the hemol-ymph of older larvae (up to 24-fold higher in 5-day oldcompared to 3-day old larvae), yet it has no likely homologsor discernable functional domains, bearing only ... primarily by drastic increases in secreted hexamerins.Consequently, lower abundance proteins become harder todetect in this background. Although the protein concentra-tion in hemolymph changes...
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