... Aco1,aconitase 1; Agt), alanine-glyoxylateaminotransferase knockout; Aldh2, aldehydedehydrogenase 2; Car3, carbonic anhydr-ase 3; Cat, catalase; Dao1,D-amino acidoxidase 1; Eno1, enolase ... CEBA-HUC-approved protocols. Immediately afterbrain death, laparotomy and bilateral thoracotomy wereperformed and the organs were harvested, making sure thatthe animals did not suffer at any stage ... Coomassie blueR-250 for preparative gels [11] or silver nitrate for analyti-cal gels [12].Image capture and analysisGels were scanned using a UMAX scanner (AmershamBiosciences, Barcelona,...