... melanoma patients since the 1960s[11,12]. After cleaning the skin with acetone, 2000 and 50 μgofDNCBin100μl of acetone were layered onthe skin of the volar aspect of the forearm, and eachdose ... tumor.Immunological MonitoringSkin TestingPrior to weekly vaccines 1 (baseline) and 4 (beginning of week 4) and at the first month follow-up visit afterEton et al. Journal of Translational Medicine 2010, ... 5min to develop the plates. Finally, the plates werewashed with tap water, dried, and the number of spots,each coinciding to a single cytokine-producing cell,was counted under a dissecting...