Báo cáo Y học: Structural heterogeneity of pyrimidine/purine-biased DNA sequence analyzed by atomic force microscopy potx
Báo cáo Y học: Structural heterogeneity of pyrimidine/purine-biased DNA sequence analyzed by atomic force microscopy potx
... PRIORITY PAPER Structural heterogeneity of pyrimidine/purine-biased DNA sequence analyzed by atomic force microscopy Mikio Kato1,2, Chad J. McAllister2, Shingo Hokabe1, Nobuyoshi Shimizu3and ... solution.Recently, we have successfully characterized the intramo-lecular triplex structure formed in supercoiled DNA within 46 bp of Pyr/Pur mirror symmetry by atomic force microscopy (AFM) accompanied by ... (AFM); H -DNA; intramolecular triplex DNA; poly-pyrimidine/ polypurine sequence. There is a wealth of evidence indicating that short pyrimidine/purine-biased (Pyr/Pur) mirror symmetrysequences...
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Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
... sites of chymotrypsin. D-Chymotrypsinogen is achimera constructed to contain a trypsinogen propeptideinstead of the Cys1–Cys122-linked wild-type chymotryp-sinogen peptide. D-Chymotrypsinogen ... size of the major peptide fragments, designated by Romannumbers, in the sequence of chymotrypsinogen. (B) Analysis with SDS/PAGE of the peptide fragments generated by D-chymotrypsin digestion of ... Sequence alignment of theinterdomain and autolysis loops of mammalian chymotrypsins and trypsins.The enzyme that was used in this study, ratchymotrypsin B, is highlighted by bold type.The loops...
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Báo cáo Y học: Structural analysis of Francisella tularensis lipopolysaccharide potx
... 2-N-(3-O-acyl4-acyl2)-b-GlcN-(1–6)-2-N-acyl1)3-O-acyl3-GlcN whereacyl1,acyl2and acyl3are 3-hydroxyhexadecanoic or 3-hy-droxyoctadecanoic acids, acyl4is tetradecanoic or (minor)hexadecanoic ... Acyl1,acyl2and acyl3residues had hydroxy or acyloxy groups at C-3 (13C signals of C-3 at 69.0–72.4 p.p.m.), while acyl4had no substituents.The signals of acyl chains could only be identified ... N-acylated with acyl2. All acyl C-1 signals wereidentified from H-2:C-1 HMBC correlations. C-1 of acyl2gave HMBC correlation to H-2 of GlcN B; C-1 of acyl4showed correlation to H-3 of acyl2....
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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot
... separation was achieved by a linear gradient of 2–600 mMNaOAc over a time of 70 min. Fractions were analyzed by analytical HPAEC and appropriately com-bined. Desalting was achieved by gelfiltration ... describedpreviously [13].Preparation of deacylated LPS of E. coliF653LPS (2.11 g) was de-O-acylated by mild hydrazinolysis(yield 1.425 g) and 902.5 mg were further subjected toalkaline de-N-acylation ... [11].Preparation of deacylated LPS of E. coli2513LPS (5 g) was de-O-acylated by mild hydrazinolysis [7](yield 3.84 g) and 400 mg of the latter were subjected toalkaline de-N-acylation as described...
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Báo cáo Y học: Structural determination of lipid A of the lipopolysaccharide from Pseudomonas reactans A pathogen of cultivated mushrooms doc
... slightacidity and the product extracted in chloroform. Aftermethylation with diazomethane it was analysed by GLC-MS.The absolute configuration of 2-hydroxy and 3-hydroxyfatty acids was determined by ... wereassigned to tetracyl species with two acyloxyacyl amides inwhich the secondary fatty acids are C12:0 (2-OH) or C12:0.Thus, the hydrolysis of only these two primary fatty acidslinked as esters ... secondary fatty acid on the proximalGlcN I was inferred by MALDI-TOF of the de-O-acylatedproduct with ammonium hydroxide at 20 °C for 18 h. Thismild procedure is able to split the acyl and acyloxyacyl...
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Báo cáo Y học: Structural determination of the O-chain polysaccharide from Agrobacterium tumefaciens, strain DSM 30205 pot
... stream of air and the mixture of peracetylated derivatives analysed by GC-MS. Absoluteconfigurations were deduced by analysis of the chiral 2-octylderivatives according to the procedure of Leontein ... ionization energy of 70 eV and a ionizingcurrent of 0.2 mA.Glycosyl-linkage analysis of LPS, was performed accord-ing to the procedure of Sandford [10]. The permethylatedlipopolysaccharide was ... to 27 mg (5.8% yield respectdry cells) of LPS fraction.Chemical composition analysisMonosaccharides were analysed as acetylated methyl gly-coside derivatives and lipids as methyl esters, according...
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Báo cáo Y học: Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 potx
... amounts of sialylated Hex3 glycoforms were indicated.bMinor amounts of Hex2 and Hex3 glycoforms lacking acetyl groups orcontaining a second glycyl group were indicated.cVery minor amounts of ... complete removal of acetate and glycine, con-firming glycine to be e ster-linked.Information on the location of the a cetyl and glycylgroups as well as glycose sequence was provided by ESItandem ... DQF-COSY and TOCSY spectra of OS-1in the region d 2.20–1.66. A s observed earlier [11], severalanhydro-forms of K do are formed during the hydrolysis by elimination of phosphate or pyrophosphoethanolaminefrom...
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Báo cáo Y học: Defective translocation of a signal sequence mutant in a prlA4 suppressor strain of Escherichia coli doc
... translocation is supplied by thehydrolysis of ATP by SecA and by the proton-motive force Correspondence to J. Tommassen, Department of MolecularMicrobiology, Utrecht University, Padualaan 8, 3584 ... acid and analyzed by SDS/PAGE and autoradio-graphy. The precursor and mature forms of PhoE are indicated by pand m, respectively.Fig. 5. Processing of (G-10L)prePhoE is impaired in secY40 cells. ... were analyzed, after immunoprecipitation with antibodies against PhoE, by SDS/PAGE and autoradiography. The precursor and mature forms of PhoE are indicated by p and m, respectively. (B) Cells of...
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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx
... indiverse ways [2]. In general the macrostructure of phyco-bilisomes consists of a core (constituted by allophycocyanin)surrounded by phycocyanin and phycoerythrin (whenpresent) organized in a periferic ... ratio of phycocyanin to phycoerythrin in rods of certain phycobilisomes to improve light harvesting [8].Furthermore, modulation of the energy levels of the fourchemically different bilins by a ... process of damage, whichare well observed by HPLC after 3 h of UV-B irradiation or3 d ays of starvation, but not by SDS/PAGE analysis.Moreover, by the HPLC-ESI-MS method, a minimummanipulation of...
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Báo cáo khoa học: Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase docx
... Biosynthesis of riboflavin.Enzymatic formatio n of 6, 7-dimethyl-8-ribityllumazine by heavyFig. 8. Stereo view of the active sites of the S. pombe 6,7-dimethyl-8-ribityllumazine synthase W2 7Y ... X-ray structure of W2 7Y mutant 6,7-dimethyl-8-ribi-tyllumazine synthase – the pentameric assembly. Pentameric assembly of the 6 ,7- dim eth yl- 8-r ibit yl lumazine synthase m utant W2 7Y fromS. ... Berlin,Germany;4Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN, USAThe a mino acid residue tryptophan 27 of 6,7-dimethyl-8-ribityllumazine synthase of the yeast...
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