Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt

... histidine phosphorylation in eukaryotes has been sparsely studied compared to protein serine/threonine and tyrosine phosphorylation. In an attempt to rectify this by probing porcine liver cytosol with ... results provide a new tool for studying eukaryotic histidine phosphorylation/ dephosphorylation. Keywords: dephosphorylation; N -phosphorylation; phos- phoamidase; phosphopeptide; pr...

Ngày tải lên: 21/02/2014, 01:21

... similar to that of the type I (single multifunctional enzymes) and type II (multienzyme systems) polyketide synthetases (PKS; data not shown) [33,34]. In contrast, its similarity to type III ... antifungal (blasticidin S, mildiomycin), antiviral (oxetanocin, Ara-A), antitumoral (oxanosine, neplanocin A), herbicidal (poly- oxins), insecticidal (nikkomycins), inmunostimulative and inm...

Ngày tải lên: 21/02/2014, 01:21

... Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II Thierry Dubois 1 , Preeti Kerai 2, *, Michele Learmonth 1 , Andy Cronshaw 1 and Alastair Aitken 1 1 The ... fter phosphorylation by C KIa and purification by HPLC. The radioactivity was released in Fig. 1. Phosphorylation of centaurin-a 1 , 14-3-3, syntaxin-1...

Ngày tải lên: 22/02/2014, 04:20

... separated by SDS/PAGE and immunoblotted with anti-UP12 Ig and anti-GroEL Ig, respectively. Quantification of protein bands was performed by scanning densitometry, as described in Experimental procedures. ... GatY and UP12, respectively. GatY ( D -tagatose-1,6-bis-phosphate aldolase of class II) , is a homotetrameric protein consisting of 31-kDa subunits; it belongs to a family...

Ngày tải lên: 22/02/2014, 07:20

... DNAs and RNAs. Fig. S3. Binding affinity of EWS and RBD to Htelo. Fig. S4. In vitro arginine methylation of RGG3 by PRMT3. Fig. S5. Identification of significant residues at RGG3 for rHtelo binding ... EAD domain therefore inhibited the high-affinity Htelo binding of RGG3 and RBD. The ability of RGG3 and RBD to repress transcription activation by EAD raised the possibility...

Ngày tải lên: 15/02/2014, 01:20

... [54,70]. In humans, T-bet gene expression is found to be rapidly induced by IFN-c in lymphoid and myeloid cells [71], but not by IFN-a, LPS or IL-1, indicating that the action of IFN-c is specific. In ... within the microenvironment, which, in turn, influence transcription factors and key signalling path- ways [8]. Th1 differentiation is initiated by coordinate signalling through th...

Ngày tải lên: 16/02/2014, 09:20

... were identified in M. catarrhalis. The acyloxyacyl-linked secondary acyl chains of the lipid A moiety of the LOS are important in some biological activities of M. catarrhalis. Elucidation of lipid ... E. coli lipid A biosynthesis involve the addition of lauroyl and myri- stoyl residues to the distal glucosamine unit, generat- ing acyloxyacyl moieties. The E. coli lauroyl and myri...

Ngày tải lên: 18/02/2014, 14:20

... an intrinsic histone acetyltransferase activity, which facili- tates transcription by decreasing chromosome conden- sation through histone acetylation and by increasing the accessibility of the ... domain of SmSmad1B contains a typical C-terminal SSVS phosphorylation motif, which is the site of phosphorylation by type I receptors of the TGFb superfamily (Fig. 1A). The pr...

Ngày tải lên: 18/02/2014, 16:20

... the phylogeny. The present study cannot exclude that clades I and II could have a common origin with cytosolic CAI, CAII, CAIII, CAVII and mitochondrial CAV vertebrate isoforms, as previously suggested ... in black and grey, respectively. Histidine residues involved in zinc binding in the catalytic site are indicated by a ‘Z’; important amino acids involved in the hydrogen bond ne...

Ngày tải lên: 18/02/2014, 16:20

... not significant. Overexpression of cystatin B, non-selenium glutathione peroxidase and translation initiation factor eIF-2B transcripts was limited to a relatively short time-window at days 14 and ... proteolysis, was strongly expressed, but only in gills. The upregula- tion of CTSL, combined with the downregulation of its reversible binding inhibitor cystatin B, implies that active...

Ngày tải lên: 18/02/2014, 16:20