studies of loose protein aggregates by flow field flow fractionation fff coupled to multi angle laser light scattering malls
Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... binding of cAMP to the anti-cAMP-binding sites of the protein The distance between the sulfur atom of Cys178 and the C9C10 bond of the indole ring of Trp85, derived from the crystal structure of CRP(cAMP)2 ... the mobility of helix A of the protein As Trp13 is located in the vicinity of the activation region AR2 of the protein [1], which is responsible for the activation of the second class of E coli ... the movement of the C-terminal domain of CRP by % A towards the N-terminal domain, which in consequence leads to rearrangement of DNA-binding domains and cAMPbinding domains of the protein This...
- 11
- 494
- 0
Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
... lifetime instead of the values of lifetimes of the resolved components Binding of cAMP to anticAMP-binding sites leads to signicant changes in the uorescing properties of Trp13 of CRP(cAMP)2, including ... resonance study of the cyclic AMP receptor protein (CRP): assignments of the NH protons of histidine and tryptophan residues and the effect of binding of cAMP to CRP Biochem J 107, 304309 14 Sixl F, ... two forms of the protein account for 55% and 45% of the total uorescence emission, respectively In contrast to the Trp13 residue, the tryptophan located at position 85 is characterized by one distinct...
- 14
- 400
- 0
Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc
... interferes with the detection of [14C] pyruvate Instead, the rate of pyruvate formation was determined by monitoring the LDH -coupled oxidation of NADH to NAD+ The coupled assay also demonstrated ... able to propose the catalytic mechanism of the reductive carboxylation of acetyl-CoA (Fig 5) (1) The TPP carbanion is generated by proton extraction from C2 carbon atom of the thiazolium ring by ... transfer electrons to ferredoxins in vivo is of particular importance for the improvement of this coupled assay and also with respect to obtaining a deeper understanding of the metabolism of H thermophilus...
- 10
- 619
- 1
Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx
... Kinetic models of multisite phosphorylation C Salazar and T Hofer ¨ A B Fig Plasticity of the stimulus sensitivity depends on protein protein interactions Contour plots are given of the effective ... and pathways of phosphorylation and dephosphorylation It will be an aim of this study to elucidate the consequences of processing order for the regulatory properties of the target protein We now ... phosphorylation The number of phosphorylation sites only comes into play through the constant factor HN Phosphorylation of multisite targets is achieved much faster by a random mechanism than by a sequential...
- 16
- 470
- 0
Báo cáo vật lý: "MORPHOLOGY STUDIES OF POROUS GaP, SYNTHESIZED BY LASER-INDUCED ETCHING" pdf
... dimension of 50 to 80 nm It appeared that the high laser power density along with long irradiation time led to dissolution of already formed structure in the porous layer due to the availability of ... of the properties of GaP could be due to an intensification of the electron-phonon interaction in the submicron to nanometer size structures of the porous layer.18 Journal of Physical Science, ... on to the sample of 1.5 mm diameter by using a suitable quartz lens with focal length of 10 and cm of diameter This lens was mounted on a micrometer holder for the focusing adjustment The laser...
- 14
- 394
- 0
Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
... Patterson figure of ˚ merit was 19 (resolution cut-off 3.0 A) Subsequent phasing with shelxe resulted in a correlation coefficient of 54% and allowed us to build a model of the protein into the Table ... calculated structure factor amplitudes, respectively Rfree is an R-factor for an unrefined subset of the data (5% of the data) a the metal ions are close to the B-factors of the coordinating ˚ residues ... relevant to proteins II Angles at the metal atom, additional weak metal–donor interactions Acta Crystallogr D 56, 857–867 36 Harding MM (1999) The geometry of metal–ligand interactions relevant to proteins...
- 10
- 542
- 0
Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
... synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble proteins into soluble ... suppressed by inhibitors of transaminases and glutamine synthase [20] Among the multitude of metabolic enzymes present in the cell extract, only those leading to transfer of [15N]-amino groups to other ... amino protons of amino acids exchange too rapidly at neutral pH to yield a signal observable in [15N]-HSQC spectra It is thus possible to obtain clean NMR spectra N-labelled proteins by cell-free...
- 6
- 461
- 0
Studies of shrimp white spot syndrome virus by protein array platform and proteomics approaches
... as time-offlight (TOF) to separate ions of a particular m/z value that then strike the detector The detector produces a magnitude of the current that is used to determine the m/z value of the ... structural proteins may help the viral nucleocapsid to move toward the host nucleus Several viral proteins were found to interact with shrimp cytoskeleton and nuclear proteins separately by the protein ... Protein array of WSSV proteins with shrimp cytoskeleton proteins 76 Figure 4.5 Protein array of WSSV proteins with shrimp nuclear proteins 77 Figure 5.1 Electron micrographs of negatively...
- 136
- 255
- 0
Inactivation of Giardia lamblia cysts by UV irradiation in real field waters
... number of Giardia lamblia cysts dosed into each animal 2: Infectivity Response is the number of animals with living stages of Giardia present/total number of animals infected The results of this ... the extent and kinetics of inactivation of G lamblia cysts by LP UV in both media were relatively similar to each other Meanwhile, the inactivation of G lamblia cysts by MP UV in the filtered ... The MPN was presented as number of infectious cysts per total number of cysts doses per animal (counted by hemocytometer during each experiment) - 42 - Journal of Water and Environment Technology,...
- 5
- 346
- 0
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... until D600 of 1.2 was reached followed by down-tempering to 18 °C for h in water bath Expression was induced by the addition of isopropyl thio-b-d-glactoside with a concentration of 0.5 mm and ... the spectrophotometric methods Because the enzymatic activity of PRTFDC1 towards hypoxanthine and guanine is only 0.26% and 0.09%, respectively, of the activity of HPRT, the role of the PRTFDC1 ... carbonyl Binding of a xanthine base from XMP in the same position, would lead to the loss of a hydrogen bond and a less favorable interaction To investigate this further, the protein was heated...
- 11
- 770
- 0
Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... via one water molecule to the O3 atom of pantoate, and via two water molecules to the O4 atom of pantoate The Ne atom of His37 is hydrogen-bonded to the O4 atom of pantoate via a water molecule, ... turn hydrogen-bonded to a network of water molecules that are hydrogen-bonded to the O1 atom of pantoate in site I The O4 atom of pantoate in site II and the O1 atom of pantoate in site I are ... hydrogen-bonded to the O4 atom of pantoate via a network that consists of two water molecules The backbone carbonyl oxygen of Phe56 is also hydrogenbonded to the O4 atom of pantoate via one of the water...
- 16
- 791
- 0
Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... been shown to regulate activity in vitro [9,11] In contrast to the relatively large number of studies devoted to the elucidation of the phosphorylation sites in HSL and the regulation of its translocation, ... the presence of the given concentrations of the hydrophobic probe bis-ANS, using TO as substrate The activity of phosphorylated HSL in the TO assay was normalized to the activities of nonphosphorylated ... protein was purified by anion exchange chromatography followed by nickel affinity chromatography and dialysis (Fig 1) Western blot analysis confirmed the identity of the purified protein as HSL (data...
- 11
- 562
- 0
Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx
... of macrophages by microbes or their cellular components induces the release of different inflammatory mediators Stimulation of TLR leads to activation of a series of signalling proteins, and to ... blocked by pertussis toxin [10] Also, TLR4-induced ERK1 ⁄ phosphorylation is inhibited by dominant-negative Gai protein constructs [11] G proteins are located downstream of G -protein- coupled receptors ... modulated by lipopeptides and LPS 10 11 12 13 14 15 16 17 658 S Riekenberg et al of regulator of G protein signaling proteins in dendritic cells: implications for G protein- coupled receptor signaling...
- 11
- 569
- 0
Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc
... able to bind to the membrane of cells Inhibitory effects of VVA1 on the hemolytic and cytotoxic activity of VVA2 The effects of VVA1 on the hemolytic activity of VVA2 were studied by incubation of ... cytotoxicity of VVA2 by preventing the binding of VVA2 to the cell membrane Interactions between VVA1 and VVA2 B Fig Effects of volvatoxin A1 (VVA1) on the hemolytic and cytotoxic activity of ... novel toxin regulator of VVA2 A B Fig Peptide competition assay (A) Schematic representation of peptide competitors (B) Binding of volvatoxin A2 (VVA2) to volvatoxin A1 (VVA1) was inhibited by the...
- 12
- 585
- 0
Tài liệu Báo cáo khoa học: Interferon-a induces sensitization of cells to inhibition of protein synthesis by tumour necrosis factor-related apoptosis-inducing ligand ppt
... sensitivity of cells to inhibition of protein synthesis by TRAIL We have previously shown that protein synthesis is rapidly downregulated following exposure of cells to TRAIL and other inducers of apoptosis ... cascade of activation of effector caspases that ultimately leads to the multiple changes in cells characteristic of TRAIL-induced apoptosis [27] The process of activation of caspase-8, and the ... ability of TRAIL to regulate the initiation of translation at the level of eIF2a phosphorylation and 4E-BP1 dephosphorylation Control of protein synthesis by IFNa and TRAIL Results Effects of IFNa...
- 11
- 679
- 0
Tài liệu Báo cáo khoa học: Impact of the native-state stability of human lysozyme variants on protein secretion by Pichia pastoris doc
... less stable proteins is a result of degradation of partially folded or misfolded proteins by the quality control system during secretion In light of the correlation between secreted protein levels ... activity of the native protein differed for each variant (ranging from 65 to 100% of WT), the quantity of lysozyme produced was determined in each case by comparing the rate of lysis to standard ... shown to be thermally destabilized with respect to WT protein, although, this does not appear to have a detrimental effect on protein expression in yeast [38,39] Investigations of the effect of...
- 10
- 577
- 0
Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... (Fig 3) The possibility of interference from the endogenous agmatinase of the bacterial vector was excluded by the DEAE-cellulose chromatographic step of the purification protocol In fact, like for ... were corrected by substracting the spectrum of the buffer solution (5 mm Tris ⁄ HCl, pH 7.5) in the absence of protein The stability to thermal inactivation was examined by incubation of the enzymes ... produced by agmatine hydrolysis, in studying the inhibitory effect of agmatine on arginine hydrolysis, reactions were followed by measuring the formation of ornithine, determined by the method of Chinard...
- 9
- 651
- 0
Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
... regulated by cytosolic injections of PKA activators and inhibitors, suggesting it to be the basis for the functional effects of cAMP injections on GIRK currents PKA-induced phosphorylation of the ... Europe GmbH, Vienna, Austria) Incorporation of radioactive 32P into the protein was quantitated and normalized to the total amount of protein as detected by the Comassie stain (= relative specific ... site(s) had been To identify the structural determinants that are responsible for phosphorylation of GIRK1 by PKA, fusion proteins comprising truncated forms of the cytosolic parts of GIRK1 and the...
- 9
- 403
- 0
Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
... Fluorescence studies of RepA R E M Diederix et al Binding of C160RepA to operator and iteron sequences followed by AEDANS uorescence The uorescence of AEDANSC160 was studied as a function of DNA concentration ... to serve as an indicator of stoichiometry In the case of 1IR, the t resulted in a binding stoichiometry of : 1, i.e dimer binding The reactant concentrations were too high to obtain relevant information ... by the apparent lack of contribution of Tyr to the excitation spectrum of acceptor AEDANS indicated in the excitation difference spectra seen in Fig 3AC Second, the Forster ă and donor-acceptor...
- 15
- 431
- 0
Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... fusion protein The human M2 receptor is a Gai ⁄ o -coupled receptor To test whether M2 can activate GOA-1, a fusion Human GPCR activates nematode G protein protein of M2 mutant and GOA-1, myc-M2(N-D)I3del::GOA-1, ... TA (2005) Use of Caenorhabditis elegans Ga chimeras to detect G -protein- coupled receptor signals J Biomol Screen 10, 127–136 Gether U & Kobilka BK (1998) G protein- coupled receptors J Biol Chem ... O & Sadee W (1994) Activation of a GTP-binding protein and a GTPbinding -protein- coupled receptor kinase (beta-adrenergic-receptor kinase-1) by a muscarinic receptor m2 mutant lacking phosphorylation...
- 9
- 400
- 0
- goods rights and other resources controlled by the company as a result of past events and from which future economic benefits are expected to flow to the company
- field studies of mountainous terrain
- field studies of riverine terrain
- field studies of coastal terrain
- field studies of glacial and dune terrain
- field studies of karst terrain
- protein engineering in structure function studies of viper apos s venom secreted phospholipases a2
- mlif on the activation and production of intracellular cytokine and chemokine receptors in human t cd4 lymphocytes measured by flow cytometry sara rojas dotor
- definitions of assisted living used by various researchers in the field
- analysis of fluorescent protein expressing cells by flow cytometry
- the detection of nk cell alloreactivity by flow cytometric cd107a assay
- functional studies of ribosomal protein mutants from salmonella enterica serovar typhimurium
- hollow fiber flow field flow fractionation a pipeline to scale down separation and enhance detection of proteins and cells
- analysis of prions by field flow fractionation
- isolation and characterization of cells by dielectrophoretic field flow fractionation
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu