analysis of fluorescent protein expressing cells by flow cytometry

... specimens were retrieved for analysis of TILs, CD8+ T cells and PD-L1 and PD-1 expression Flow cytometry for expression of ICPs on circulating T cells PBMCs were stained with fluorescent antibodies ... could be concurrently thawed and analyzed by flow cytometry Assessment of ICP expression on CD4+ and CD8+ T cells was performed at baseline and at the time of the last chemotherapy treatment Archived ... LSR-II flow cytometer and data was analyzed with FlowJo software (Tree Star, Inc.) Differences in the expression of ICP receptors before and after NAC were determined by comparing the percentage of

Ngày tải lên: 30/05/2020, 22:02

... area of fluid dynamics and heat transfer The partial slip occurs in the most of the microfluidic devices since slip flow happens if the characteristic size of the flow system is small or the flow ... characteristics of heat transfer and fluid flow due to forced convection of steady-laminar flow of an incompressible fluid inside channel with circular cross-section and channel made of two parallel ... plates was analyzed by Mahmud and Fraser [30] The analysis of the second law of thermodynamics due to viscoelastic MHD flow over a continuous moving surface was presented by Aăboud and Saouli

Ngày tải lên: 02/11/2022, 09:24

... proliferation of several phenotypes of cells is medi- ated by growth factor or cytokine induced mitogen-acti- vated protein kinases (MAPKs), a family of serine- threonine protein. MAPKs consist of extracellular ... expression of CTGF gene and protein. The TGF-β1 induced phosphorylation of Smad 2/3 and the expression of CTGF mRNA and protein were all blocked by the inhibition of ERK and JNK, but not by the inhibition ... involvement of MAPKs in TGF-β1 induced proliferation of ASMCs, the cells were pretreated for one hour with 10 µM of SB 203580, 50 µM of PD 98059, or 10 µM of SP 600125, selective inhibitors of p38,

Ngày tải lên: 12/08/2014, 16:20

... con- cept of protein interaction modules as fundamental func- tional units was first outlined by Hartwell et al. [12]. Protein interaction modules are composed of a variable number of proteins, ... number of definitions of community (or protein interaction module in terms of the protein interaction network) have been proposed with differ- ent criteria [14,17,22]. No clear consensus of module ... number of common interacting partners highly correlates with the functional association of two proteins [24,25]. In a species with N proteins, the number of distinct ways in which two interacting proteins

Ngày tải lên: 14/08/2014, 08:20

... classification of proteins solely on the basis of protein- protein interaction data, independently of sequence data. It clusters proteins with respect to their common interactors and defines classes of proteins ... of duplicated genes using a bioinformatic analysis of the protein- protein interaction network in which the products of these genes are involved, and to provide detailed protein ... frame of evolutionary studies, estimated the rate of evolution of proteins according to their number of interactors [31] and addressed the issue of the link between protein dispensability

Ngày tải lên: 14/08/2014, 14:21

... thesis/dissertation prepared By Entitled For the degree of Is approved by the final examining committee: Chair To the best of my knowledge and as understood by the student in the Research ... ____________________________________ Approved by: Head of the Graduate Program Date Alexandra Nicole Mendlein Instrumental and Chemometric Analysis of Automotive Clear Coat Paints by Micro Laser Raman and UV ... Analysis of Automotive Clear Coat Paints by Micro Laser Raman and UV Microspectrophotometry Master of Science Alexandra Nicole Mendlein 06/30/2011 i INSTRUMENTAL AND CHEMOMETRIC ANALYSIS OF AUTOMOTIVE

Ngày tải lên: 24/08/2014, 13:44

... over-the-counter market, from the hands of the unocial brokers to the banks. Because of low volume and lack of coordination be- tween the banks, a delay of several days or weeks often occurred before orders ... reports the probabilities of di€erent types of orders and trades occurring given the previous state of the book The state of the. .. for the number of shares and volume for ... number of shares available in the market at the open. This is fol- lowed by a continuous auction market, where marketable orders by public in- vestors are transacted with the limit orders of other

Ngày tải lên: 31/10/2014, 10:22

... and Thrust/Drag Analysis of Improved Fish Robots Actuated by Piezoceramic Composite Actuators Nguyen, Quang Sang Thesis of the Doctor of Philosophy Department of Advanced Technology ... the Doctor of Philosophy Department of Advanced Technology Fusion Graduate School of Konkuk University Design, Fabrication and Thrust/Drag Analysis of Improved Fish Robots Actuated by Piezoceramic ... Department of Advanced Technology Fusion and the Graduate School of Konkuk University in partial fulfillment of the requirements for the degree of Doctor of Philosophy August 2010 Approved by

Ngày tải lên: 13/11/2014, 08:24

... frequency of occurrence of reference used by Obama and Romney 19 Table 3: The frequency of occurrence of substitution used by Obama and Romney .21 Table 4: The frequency of occurrence of ellipsis ... ellipsis used by Obama and Romney 23 Table 5: The frequency of occurrence of conjuction used by Obama and Romney .25 Table 6: The frequency of occurrence of reiteration used by Obama and ... the overall picture of the occurrence of reiteration is shown in the table below. Types of collocation Frequency of occurrence Frequency of occurrence used by Obama (%) used by Romney (%) Lexical

Ngày tải lên: 13/09/2015, 22:15

... ANALYSIS OF GONAD DIFFERENTIATION IN ZEBRAFISH BY HISTOLOGY AND TRANSGENICS WANG XINGANG NATIONAL UNIVERSITY OF SINGAPORE 2007 ANALYSIS OF GONAD DIFFERENTIATION IN ZEBRAFISH BY HISTOLOGY ... Differentiation of Sertoli cells 1.7.2 Differentiation of Leydig cells . 1.7.3 Differentiation of primordial germ cells ... ring finger protein 128 isoform 2, partial [Danio rerio] similar to Transgelin isoform [Danio rerio] hypothetical protein LOC368901 [Danio rerio] similar to Hyperion protein, 419 kD isoform, partial

Ngày tải lên: 14/09/2015, 09:39

... also yielded a list of cancer-associated proteins that were regulated after treatment thus suggesting the reversal of the malignant phenotype of HCC cells by butyrate. A group of proteins involved ... antiapoptotic proteins such as XIAP Hitherto, one of the key characteristics of cancer cells is its loss of differentiated... treatment The immense advantages of proteomics offered by 2DE ... contains heparin-bound proteins eluted at 0.8 M NaCl. Figure 4.5: 72 Representative images of 2D-DIGE analysis of (A) unbound and (B) heparin-bound protein fractions. 120 µg of proteins from Cydyelabeled

Ngày tải lên: 15/09/2015, 22:42

... Thrust/Drag Analysis of Improved Fish Robots Actuated by Piezoceramic Composite Actuators Nguyen, Quang Sang Thesis of the Doctor of Philosophy Department of Advanced Technology Fusion Graduate School of ... Thrust/Drag Analysis of Improved Fish Robots Actuated by Piezoceramic Composite Actuators Nguyen, Quang Sang Thesis of the Doctor of Philosophy Department of Advanced Technology Fusion Graduate School of ... School of Konkuk University in partial fulfillment of the requirements for the degree of Doctor of Philosophy August 2010 Approved by Park, Hoon Cheol Major Advisor Table of Content List of Figures

Ngày tải lên: 11/06/2016, 12:15

... distribution of healthy cells is approximated by Correlation of fluorescence and cell size Regardless of the presence of fluorochromes, every cell emits light when it is excited by a laser - ... complete work flow from raw flow cytometry data to a list of genes that are components of or interact with the cellular process of interest Procedures (methodologic recommendations as well as software) ... contained cells transfected with a different overexpression construct Along with the phenotype of interest, the amount of overexpression of the respective proteins was recorded via a fluorescent...

Ngày tải lên: 14/08/2014, 17:22

... Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae ... as fluorescent dye allowing determination of the threshold cycle at which exponential amplification of PCR products begins Specificities of amplicons were confirmed by melting curve analyses by ... 6-carboxyfluorescein by the esterases of living cells to label viable cells This combination allows the differentiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5...

Ngày tải lên: 09/08/2014, 14:22

... determined by p24 ELISA Panel B: Time course of cell adhesion by HIV particles Binding of HIV(CD4) particles to Env+ cells (ᮀ), Env- cells (●), or adhesion of HIV(w/o CD4) particles to Env+ cells ... numbers (104 to × 104) of Env- BJAB cells, the parental cells of TF228 As shown in Figure 6, addition of increasing numbers of Env- cells dramatically decreased the number of neomycin resistant ... high At a ratio of Env+ to Env- cells, the transduction of Env+ cells was inhibited by over ninety percent (Figure 6) HIV-1 is a cytolytic retrovirus and the number of cells expressing HIV-1...

Ngày tải lên: 13/08/2014, 09:21

... substitution of a noun as head of a noun phrase by one or ones, or the substitution of a whole phrase by the same 15 - Verbal substitution is by means of “Do” “Do” operates as head of a verbal ... Concept of Discourse Analysis The notion of discourse analysis is defined in many ways: Stubbs (1983:1) defined “discourse analysis as (a) concerned with language use beyond the boundaries of a ... the primary interest of discourse analysis in this study is to give an account of how forms of language are used in making speeches on the reality TV shows The analysis of discourse here would...

Ngày tải lên: 26/11/2013, 12:41

... were selected MALDI-TOF/TOF In addition to LC-MS ⁄ MS analysis, we also completed TOF ⁄ TOF analysis of selected peaks to confirm structure using a MDS Sciex 4800 MALDI-TOF ⁄ TOFÔ instrument (Applied ... patterns Glycan analysis of cRBCs Analysis of cRBC glycans by 1H-NMR To determine the most relevant glycan sequences for each composition, we completed additional MS and NMR-based analysis of the cRBC ... species at m ⁄ z of 2792.2 and 3083.8 by the release of one and two sialic acids, respectively Quantitative 1H-NMR analysis of this sample also indicates the presence of a mixture of both a2 fi and...

Ngày tải lên: 14/02/2014, 19:20

... possibility of a saturable mechanism of entry (Fig 1A); identical results were obtained with a control ODN (not shown) Examination of the cells by light microscopy showed that untreated cells, cells ... and 48 h (Fig 1B); after 72 h, the amount of dead cells and debris made it difficult to count dead cells Analysis by flow cytometry clearly showed the cells that had incorporated hpST3dODN (FITC ... activity of STAT3 and colocalizes with STAT3 to the cytoplasm of SW480 cells The transcriptional activity of STAT3 after treatment of the cells with hpST3dODN was analysed in SW480 cells transfected...

Ngày tải lên: 18/02/2014, 08:20

... Administration, Office of Advocacy from data provided by the U.S Department of Commerce, Census Bureau, Business Dynamics Statistics Using a different type of analysis defining firms by just their ... Administration, Office of Advocacy, from data provided by the U.S Department of Labor, Bureau of Labor Statistics, Business Employment Dynamics Firm Size Much of the preceding discussion of job creation ... Administration, Office of Advocacy, from data provided by U.S Department of Commerce, Census Bureau, Statistics of U.S Businesses which covers the private-sector Small businesses’ share of private-sector...

Ngày tải lên: 23/03/2014, 05:22

... degree of Nat5 inhibition observed in the cells The cell cycle disruption induced by the downregulation of Nat5 expression is induced by the activation of p53 that induces an increase of p21 ... absence of a specific marker One of the general features of tumour cells consists of changes in their cell surface, which can be also reflected in the glycosylation pattern of their secreted glycoproteins ... Bickle2 Laboratory of Dr Ettrich, Institute of Physical Biology of USB and Institute of Landscape Ecology of AS CR, Nove Hrady, Czech Republic, 2Laboratory of Dr Bickle, Department of Microbiology,...

Ngày tải lên: 30/03/2014, 20:20