functional studies of ribosomal protein mutants from salmonella enterica serovar typhimurium
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... quantitation of microgram quantities of protein utilizing the principle of protein dye binding Anal Biochem 72, 248–254 Knecht W, Bergjohann U, Gonski S, Kirschbaum B & Loffler M (1996) Functional ... phosphorylation of dGuo was reduced markedly Structure of Dm-dNK-E52D in complex with dTTP The structure of one of the mutants, E52D, was solved ˚ using X-ray crystallography at a resolution of 2.5 A ... 1-(b-d-arabinofuranosyl)-cytosine (AraC), 1-(b-d-arabinofuranosyl)-thymine (AraT) and (E)-bromvinyl-2¢-deoxyuridine (BVDU) Mutants of catalytic residues: E52 and R105 It is evident from the kinetic...
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Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
... †National Collection of Type Culture, ‡Sal: Salmonella spp., §ST: Salmonella Typhimurium, Salmonella Enteritidis ∥ SE: Detection and quantification of Salmonella spp., Salmonella Typhimurium and Enteritidis ... serotypes Typhimurium Table Specificity test of the multiplex real-time PCR detecting Salmonella spp., Salmonella enterica serovar typhimurium and enteritidis Organisms Target organisms Typhimurium ... 21.06 *Salmonella Typhimurium, Salmonella Enteritidis, ‡not tested Detection and quantification of Salmonella spp., Salmonella Typhimurium and Enteritidis Table The evaluation of the multiplex...
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Structural and functional studies of VP9, a novel nonstructural protein from white spot syndrome virus
... 2002) genes The functional studies of WSSV proteins have been greatly hampered by the lack of a suitable cell line to act as the virus host 11 Consequently, functional analysis of WSSV proteins still ... 0.4-0.6 ml of protein solution of approximately mM concentration is required; (iii) Protein samples used must be free of contaminants arising from the NMR tube or the protein preparation The protein ... Hydrophobicity plot of VP9 46 Figure 3.3 Purification profile of His-VP9 47 Figure 3.4 MS result of native VP9 48 Figure 3.5 DLS result of VP9 49 Figure 3.6 CD result of VP9 50 Figure 3.7 CD profiles upon...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... scaled using scala from the ccp4 software suit [30] The structure was solved with the molecular replacement software molrep [31] using coordinates from a monomer of HPRT (Protein Data Bank code: ... elucidation of the structure of PRTFDC1 and the definition of its ligand binding specificity from a large metabolome library provides an initial start point for defining the molecular function of PRTFDC1 ... of 50 lL per well The plate was heated at °CÆmin)1, and images take every 0.5 °C in the range 25–80 °C Intensities (as a measure of light scattering from protein aggregation) were converted from...
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Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... indicating that the side effect of the activation of GAR-3 is limited Furthermore, the phenotype of gar-3 loss -of- function mutants is almost wildtype with the exception of a faster pharyngeal pumping ... results indicate that M2 in the GOA-1 fusion protein is functional, and the ligand-binding properties agree with that of the Gai1 fusion protein Activation of GOA-1 by muscarinic agonists Agonist-bound ... elegans The ligand-binding properties of M2 mutant::GOA-1 fusion protein were similar to those of Gai ⁄ o fusion protein In addition, GTP causes the decrease of affinity for carbamylcholine to M2...
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Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx
... genomes of a number of prokaryotes from diverse taxonomic groups with functional family II sPPase genes, thus illustrating functional substitution by nonhomologous sPPases in a context of gene ... peculiar properties of the enzymes from photosynthetic proteobacteria may result from functional adaptations to specific metabolic scenarios The sPPases from the two classes of green photosynthetic ... Josse for critical review of the manuscript, and Professor W Loffelhardt (University of Vienna, Austria) for ¨ his assistance in N-terminal protein sequencing and some MALDI-TOF MS analyses This work...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
... mixture of 10 mL taken from each of the five reaction mixtures The method consists of adding various iodoacetamide and iodoacetate ratios to portions of the protein to generate a complete spectrum of ... SDS/PAGE The protein yield from L of culture was 40 mg for all the recombinant proteins The molecular mass of the proteins was analysed by electrospray mass spectroscopy The measured mass of PfPDO ... sites Refolding of the scrambled RNase in the presence of PDI was used as a positive control, and the absence of the recovery of the RNase activity in the presence of the thioredoxin from Alicyclobacillus...
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Functional studies of BPGAP1, a novel BCH domain containing RhoGAP protein
... representation of selected human RhoGAP domain-containing preoteins Domain organization of Cdc42GAP-like proteins Molecular cloning of different isoforms of BPGAP family cDNA and protein sequences of BPGAP1 ... FUNCTIONAL STUDIES OF BPGAP1, A NOVEL BCH DOMAIN-CONTAINING RHOGAP PROTEIN SHANG XUN (M.Sc., B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... Expression profiles of BPGAP family cDNAs in various cell lines Expression profiles of BPGAP family cDNAs in various mouse organs Expression constructs of BPGAP1 and its protein expression profiles...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat approach of LPL ... Structural features of lipoprotein lipase Lipase family relationships, binding interactions, non-equivalence of lipase cofactors, vitellogenin similarities and functional subdivision of lipoprotein lipase ... structures of the lid (orange) Ó FEBS 2002 Dimeric model structure and function of lipoprotein lipase (Eur J Biochem 269) 4705 Table Heparin affinity of C-terminal domain of LPL In vitro product of C-terminal...
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Báo cáo khoa học: Protein folding intermediates of invasin protein IbeA from Escherichia coli pdf
... between protein sequences and 3D structures, the nature of protein folding pathways, the stability of proteins and the transport of proteins across membranes [10] Even though a large amount of information ... (2003) Analyses of circular dichroism spectra of membrane proteins Protein Sci 12, 875–884 Sreerama N & Woody RW (2004) On the analysis of membrane protein circular dichroism spectra Protein Sci ... view of the non-cooperative unfolding of a molten globule Nat Struct Biol 4, 630–634 17 Dalby PA, Oliveberg M & Fersht AR (1998) Folding intermediates of wild-type and mutants of barnase I Use of...
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Báo cáo khoa học: Functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan ppt
... biosynthesis of PUFAs goes through the desaturation and elongation of essential FAs taken from the diet, linoleic and a-linolenic (18:3 n-3) acids, which allow the production of n-6 and n-3 series of PUFAs, ... the Trypanosoma proteins as front-end D4 desaturases Functional characterization of front-end desaturases Functional characterization was carried out by determining the FA profiles of Saccharomyces ... of linoleic acid (18:2) and 22:5 and 22:6 PUFAs, but lower levels of oleate (18:1) and C16 FAs than the human host Procyclic T brucei showed lower levels of 16:0 and 18:1, but higher ratios of...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
... inability of the isoforms e–h to induce accumulation of cAMP suggests that functionally important domain(s) are missing in the final proteins For example, CRFR1e Ó FEBS 2004 encodes soluble protein of ... (B) The structure of constructs used for functional assays (C) The structure of constructs used for Western blotting In general the majority of our isoforms were translated into proteins (Fig 2) ... 1B) Protein expression To verify that the constructs produce proteins of the expected masses, we attached the V5 epitope to the C terminus of the CRFR1 isoforms (Fig 1C) The predicted masses of...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... expression of Rv1399c The Rv1399c gene product encodes a 318 amino acid protein with a molecular mass of 31.7 kDa and a calculated pI of 4.53 and belongs to the Lip family that consists of 21 members ... mg of protein from L of cell culture (Table 1, Fig lane 4)], we looked for optimal conditions to solubilize Rv1399c using a new refolding analytical approach by sample dilution consisting of ... the specific activity increased from 1050 to 1350 UÆmg)1 This was due to the presence of precipitated material accounting for 13% of the total protein and arising from unfolded or misfolded Rv1399c...
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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx
... of a single protein consists of a series of peaks, each of which represents a multiply charged ion of the intact Injection of the total mixture of phycobilisomes from Synechocystis PCC 6803, ... of protein 18 17 17 18 45 000 Ó FEBS 2002 Study of phycobilisomes organization by HPLC-ESI-MS (Eur J Biochem 269) 1537 molecular masses deduced from their DNA sequences of the phycobilisome protein ... involved in photodamage of proteins They arise from the fact that in hydrophobic proteins radical attacks occur preferentially in the external hydrophilic region of the protein, a region rich...
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Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate specificity doc
... reminiscent of that of human heme oxygenase-1 [13] In the present study, we present the crystal structure of the HP1287 gene product as well as one of its mutants (F47Y) and discuss the in vivo role of ... loose packing of the protein tetramers in the crystal cell, which leaves a large amount of empty space, 80% of the volume, filled with solvent The alignment of the amino acid sequence of HP1287 ... identity and 51% similarity to the TenA protein from B subtilis The 3D structure of the monomer is quite similar to that of the other members of the TenA family of known structure: twelve a-helices,...
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Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot
... on the surface of the PO and many nerve fiber tracts and varicosities in trunks of the PO Cloning and sequencing of PO-CHH cDNA The first PCR of PO cDNA with a combination of a set of primers LF1 ... revealing the presence of a long 3¢-UTR ( 1.5 kbp) The amplicon (132 bp) produced from the nested PCR of 5¢-RACE cDNA contained a 5¢-UTR and the remaining sequence of the 5¢-end of PO-CHH The complete ... condition, the amount of ES-CHH (376 ± 67 fmolÆmL)1) in hemolymph was 9-fold higher than that of PO-CHH (45.6 ± 8.6 fmolÆmL)1) One hour of exposure of hypoxia induced CHH secretion both from the ES and...
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Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf
... to GBP, but rather to that of the larger maltose-binding protein This kind of protein is exemplified by the Thermus thermophilus protein, PDB entry 2B3B [28] The mode of binding the monosaccharide ... refined X-ray structure of the periplasmic glucose ⁄ galactose receptor from Salmonella typhimurium J Mol Biol 233, 739–752 22 Luck LA & Falke JJ (1991) 19F NMR studies of the d-galactose chemosensory ... chemoreceptor protein Science 242, 1290– 1295 ˚ 25 Flocco MM & Mowbray SL (1994) The 1.9 A X-ray structure of a closed unliganded form of the periplasmic glucose ⁄ galactose receptor from Salmonella typhimurium...
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Báo cáo khóa học: Functional analyses of placental protein 13/galectin-13 pdf
... vicinity of genes of four known (galectin-10 [27], galectin-7 [28], galectin-4 [29] and placental protein 13-like Ó FEBS 2004 Functional analyses of PP13/galectin-13 (Eur J Biochem 271) 1069 protein ... order of the protein list was based upon their homology to PP13/galectin-13 sPP13, similar to placental protein 13; PP13LP, placental protein 13-like protein; CLC2, Charcot–Leyden Crystal protein; ... Assignments of proteolytic fragments from tryptic digests of PP13/galectin-13 affinity purified 38 and 41 kDa proteins Protein identification and sequencing were described in Experimental procedures Most of...
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