gene expression studies with superarray s pathway specific oligoarrays
Mycobacterium bovis, BCG modulation of gene expression in bladder cancer
... processes by GSTs GST Observations References Class Alpha • Binds azo-dyes, bilirubin, heme, steroids and thyroid hormones for disposal [130] Class M1 • Inhibits Ask1during non-stressed conditions ... development with respect to size (T), lymph nodes involvement (N) and distant metastasis (M) Most medical facilities use the TNM staging system to classify their cancer cases Figure1.1 illustrates the staging ... seems to be a key in regulating apoptosis, a response tipping towards Erk being vital for survival The redox sensitive signalling factors use cysteine residues as redox sensitive sulphydryl switches...
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PURIFICATION OF ORGANIC CHEMICALS pot
... bottles), sodium thiosulphate soln (two bottles), alkaline sodium hydrosulphite with sodium anthraquinone-2-sulphonate as indicator (two bottles), and 10% aqueous KOH soln (two bottles) The gas was ... acetonitrile with cold, satd aq KOH is undesirable because of base-catalysed hydrolysis and the introduction of water Drying by shaking with silica gel or Linde 4A molecular sieves removes most of the ... 1.4141 Distil under reduced pressure, then redistil at atm pressure It is : miscible with H20 but is slowly decomposed by it Store in dry atmosphere The 2,4-dinitrophenylhydruzone has m 115-11 5S0 (from...
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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc
... decrease by 100 steps) The two-phase solvent system was pumped with a Syknm S 1021 pump using a dual piston solvent delivery system (Syknm, Germany), equipped with HPCPC to pump Maximum sample ... was 0.34 s per scan The inlet, ionization source temperatures were 250 and 2801C [27] Results and discussions The selection of the two-phase solvent system is the first and critical step for an ... HSCCC separation Successful separation by HSCCC or HPCPC depends on the suitable selection of a two-phase solvent system, which should have good solubility and stability for target compounds,...
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PURIFICATION OF LABORATORY CHEMICALS pot
... COMPOUNDS NUCLEIC ACIDS PHENOLS POLYPEPTIDES AND PROTEINS QUINONES SALTS With metal ions With organic cations With sodium alkane sulfonates SULFUR COMPOUNDS Disulfides Sulfones Sulfoxides Thioethers ... LIQUIDS FOR DRYING PISTOLS VAPOUR PRESSURES (mm Hg) OF SATURATED AQUEOUS SOLUTIONS IN EQUILIBRIUM WITH SOLID SALTS DRYING AGENTS FOR CLASSES OF COMPOUNDS STATIC DRYING FOR SELECTED LIQUIDS (25°C) ... TECHNOLOGIES 81 SOLID PHASE SyNTHESIS SOLID PHASE PEPTIDE SYNTHESIS (SPPS) SOLID PHASE DEOXYRIBONUCLEOTIDE SYNTHESIS POLYMER SUPPORTED REACTANTS SCAVENGER RESINS COMBINATORIAL CHEMISTRy 81 82 82 82 82...
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purification of carbon nanotubes
... as-prepared MWCNTs were dispersed in water with sodium dodecyl sulfate (SDS), and a stabilized colloidal suspension was formed The suspension was filtered using a filtration apparatus with a funnel ... can also separate amorphous carbon and CNPs based on the different stabilities in dispersions consisting of amorphous carbon, SWCNTs, and CNPs in aqueous media The different stabilities resulted ... purity assessment 3.1 Synthesis methods Carbonaceous and metallic impurities are produced as byproducts during the synthesis of CNTs If pure CNTs without any impurity were directly synthesized,...
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Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot
... Phylogenetic tree of aminotransferases on the basis of the amino acid sequences The numbers at the nodes are bootstrap confidence values expressed as percentages of 1000 bootstrap replicates The ... also possessed PSOT activity, which is found in some enzymes belonging to family IV A study of the structure of the Escherichia coli PSOT identified several conserved residues that bind to the substrates ... presumed to contribute to aspartate (Asp), alanine (Ala) and glycine (Gly) syntheses Phylogenetic analysis of these enzymes showed a unique combination of substrate specificities and phylogenetic...
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armarego - purification of laboratory chemicals 5e
... phthalate esters are listed as plasticisers as well as adipates, azelates, phosphates, epoxides, polyesters and various heterocyclic compounds These plasticisers would enter the solvent during passage ... 72 Solid Phase Synthesis 72 Solid Phase Peptide Synthesis (SPPS) 73 Solid Phase Deoxyribonucleotide Synthesis 73 Solid Phase Oligosaccharide Synthesis 73 Solid ... used successfully with ion-exchange celluloses as stationary phases and various aqueous buffers as mobile phases Also, gels (e.g Sephadex G-50 to G-200 superfine) have been adsorbed on glass...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... copies in collected culture media was expressed as viral DNA copies/ml Analysis by SDS-PAGE and silver staining Samples were separated on SDS 6–15% gradient polyacrylamide gel electrophoresis (PAGE) ... Laboratories (Hercules, CA, USA) Indirect Immunofluorescent Assay Fluorescence microscopy analysis for gp60/110 was conducted as previously described [38] Transmission Electron Microscopy Negative staining ... associated proteins [17] Association of CD46 with HHV-6A viral particles has previously been indirectly shown in MS patient samples In that study, HHV-6A particles from out of 42 MS patient sera...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... copies in collected culture media was expressed as viral DNA copies/ml Analysis by SDS-PAGE and silver staining Samples were separated on SDS 6–15% gradient polyacrylamide gel electrophoresis (PAGE) ... Laboratories (Hercules, CA, USA) Indirect Immunofluorescent Assay Fluorescence microscopy analysis for gp60/110 was conducted as previously described [38] Transmission Electron Microscopy Negative staining ... associated proteins [17] Association of CD46 with HHV-6A viral particles has previously been indirectly shown in MS patient samples In that study, HHV-6A particles from out of 42 MS patient sera...
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Báo cáo hóa học: " Bacterial-based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance" doc
... 22 kDa ISEF SASKEI ISEF SASKEI ISEFGS GTFT ISEFGS GTFT ISEFGSTSLYK The N-terminus of each recombinant LASV protein cleaved with Factor Xa contains four extraneous amino acids for NP and six for ... applications for the LASV proteins generated by these studies is the development of sensitive ELISA-based immunoassays for early detection of Lassa fever in infected patients Toward this end, we ... industrial-level expression of proteins with medical relevance for humans Thus, expression technologies that exploit secretory mechanisms may alleviate difficulties encountered with proteins such as LASV NP,...
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Báo cáo hóa học: " Temperature sensitive influenza A virus genome replication results from low thermal stability of polymerase-cRNA complexes" doc
... mammalian host systems, such considerations may have significance for the influence of avian virus-derived polymerase genes on host range and pathogenesis Methods Cells, virus, plasmids, antibodies and ... RNA synthesis and viral protein expression (data not shown) Other studies have also noted inhibition of influenza virus gene expression by PGA and other prostaglandins and related compounds that ... virus polymerase with cRNA promoter is indeed significantly less stable at elevated temperatures than the interaction with the vRNA counterpart This suggests that negative strand synthesis fails...
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báo cáo hóa học: " Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007" potx
... and resuspended in sodium phosphate buffer (50 mM, pH 7.5) Cell disruption was performed by FastPrep (40 s, m /s; MP Biomedicals, Solon, OH, USA) For SDS-PAGE analysis, the supernatant was substituted ... cultivation Gene expression analysis Gene expression analysis was performed with crude cell extract Samples standardized to cell amount were taken during cultivation Cells were harvested by centrifugation ... 2,5-diketocamphane monooxygenase was successfully cloned and overexpressed recombinantly in E coli as the heterologous expression host Hence, this enzyme is now easy Kadow et al AMB Express 2011, 1:13 http://www.amb-express.com/content/1/1/13...
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purification of some heavy metals from paper making
... (ICP-AES) and analyzed by statistical software (SPSS 17.0) Instruments and reagents.The inductive coupling plasma emission spectrograph is from the US Thermal Power Company The reagents are obtained ... root tissue, Zhu H M, and others have studied absorption characteristics of Tidal flat wing alkali peng to heavy metals They also got similar results[16] Table distribution characteristics of heavy ... absorption and distribution in wetland reed Researchers such as Weis[7], Windham[8], analyzed and compared distribution of toxic heavy metals and absorbency in reed, Aksoy[9] discussed the rules of migration...
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báo cáo khoa học: "Profiling and quantitative evaluation of three Nickel-Coated magnetic matrices for purification of recombinant proteins: helpful hints for the optimized nanomagnetisable matrix preparation" pps
... capacity The sum of the percents of specific band densities in W1-4 and RF was defined as protein loss Statistical Analysis Numerical data analysis was done using SPSS software version 13.0 (SPSS Inc., ... magnetisable solid phase support has become one of the interesting issues among chromatography reagents for His-tagged protein purification due to their less susceptibility to sample viscosity, ... result in increased binding to His-like endogenous proteins as impurities Thus, it seems that the surface chemistry of the matrix is an important determinant which affects the degree of non-specific...
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Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx
... use as diagnosis antigen, for the antibody for E coli in sera effects the ELISA assay This provide us a compelling reason for expressing PPV VP2 in a baculovirus system, although baculovirus expression ... baculovirus constituents PPV VP2 has been expressed in insect cell-baculovirus systems by a number of other groups previously [7,9,14,15] PPV VP2 expressed by baculovirus in sf9 cells produced VLPs [7,9,14,15] ... recombinant VP2 specific proteins were assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot analysis The purified proteins were separated by SDS-PAGE and...
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Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
... proteins Six E coli strains were tested to obtain the best levels of expression and solubility of the RTB/p24 and p24 proteins in cytosolic fractions: HMS 174, HMS Rosetta 2, HMS LysE, BL21 Rosetta ... mice when administered via the i.n route Nevertheless, this response was not as strong as that obtained in mice immunized with p24/CT This is not surprising since CT is the mucosal adjuvant of ... HindIII p24 pTrcHis A Figure Expression vectors Expression vectors Map showing the constructs employed for E coli expression in vector pTrcHis (A) RTB/p24 and (B) p24 The histidine tag is indicated...
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PURIFICATION OF SIMPL ANTIBODY AND IMMUNOFLUORESCENCE OF SIMPL SUB-CELLULAR LOCALIZATION IN RESPONSE TO TNFα- AND IL-1
... (Hotchkiss et al 1999) Chronic inflammation contributes to the pathogenesis associated with diseases such as arthritis and atherosclerosis (Libby 2007) In breast cancer, the invasion of immune cells ... receptor associated kinase (IRAK-1) A The immune response The immune system is an organism s defense against foreign elements such as pathogens or other possibly harmful agents There are two arms of ... antibody was incubated with beads in PLC lysis buffer for hr Both negative controls as well as the lysed tissue samples were subjected to SDS PAGE and subsequent Western blotting as per the immunoprecipitation...
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studies of pathogenesis-related proteins in the strawberry plant partial purification of a chitinase-containing protein complex and analysis of an osmotin-like protein gene
... constant exposure to soil-associated microbes In addition, as described in a previous section, high expressions of TLPs were observed in flower tissues, overripe fruits, as well as seeds of several ... chitinases, and most of the gene information is related to class I, II, and III chitinases Thus, only these three classes of chitinase genes will be discussed in this section 1.2.3.1 Class I and ... Chitinase Genes The available genomic sequences for class I and II chitinases show that most genes of these two classes contain two introns The first intron is usually located after the position...
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purification of phytase from aspergillus fumigatus
... plants and microorganisms, particularly fungi Most of the studies focused on phytases produced from Aspergillus sp., they are high phytase producers (Rao et al., 2009) Among them A fumigatus is ... on Phenyl Sepharose 3.2.2 Purification of phytase by cation exchange chromatography on SP-Streamline 10 CONCLUSIONS AND SUGGESTIONS 14 4.1 Conclusions 14 4.2 Suggestions 14 REFERENCES 15 ii INTRODUCTION ... research on phytase from A fumigatus isolate found another phytase of 60 kDa (Pasamontes et al., 1997) Wyss et al., (1999) studied on phytases from six fungi strains revealed that molecular mass...
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THE CONTRIBUTION OF CLAMS ON TIDAL FLAT PURIFICATION CAPACITY
... nutrient regeneration is known as a major driving force in the dynamics of biophilic elements in coastal marine ecosystems It has been shown that the macrozoobenthos, particularly dense assemblages of ... Bran+Luebbee), respectively T-test statistical analysis was performed to distinguish the differences between pore water quality in the sediment with and without clams NO3-N was analyzed using ion chromatography ... as sources of readily biodegradable substances In the case of without clams, particulate organic matters degrade by microorganism through hydrolysis process Degradation rate of the hydrolysis...
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- 05 kaposi s sarcoma associated virus governs gene expression profiles toward b cell transformation
- control of gene expression it s how you play your cards that counts
- insights from gene expression studies during preimplantation development
- hodgkin apos s lymphoma biology new prognostic factors and outcome prediction using gene expression signatures
- transposition of t pop into the chromosome of s enterica isolation of t pop insertions affecting gene expression
- manipulation of gene expression in the central nervous system with lentiviral vectors
- primary epithelial ovarian neoplasms new concepts concerning origin pathogenesis and classification based on morphology immunomarkers molecular features and gene expression studies
- thyroid carcinoma molecular signature by histotype specific mutations and gene expression patterns
- agrin apos s roles in synapse specific gene expression
- reliable and specific protein function prediction by combining homology with genomic s g ntext
- a microarray analysis correlating gene expression with clinical outcomes
- targeted gene expression with shielded polyplexes
- sustained gene expression in vitro and in vivo by the ebv replicon vector coupled with hvj liposomes
- culture proliferation assays and gene expression studies
- ahn j h song s k choi y d lee j s 2003 expression of an expansin gene is correlated with root elongation in soybean plant physiology 131 pp 985 997
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25