insights from gene expression studies during preimplantation development
Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc
... different from other types of analysis because it quantifies the overall importance of metabolism versus gene expression before examining specific metabolites Earlier regulation analysis studies ... attributed to a single process in the gene expression cascade Discussion Time-dependent regulation analysis quantifies the relative importance of metabolism and gene expression in FEBS Journal 276 (2009) ... (Ct) values were used to calculate the relative level of gene expression of a certain gene (X) normalized to the mean of the control gene PDI1 and the steady-state sample of the chemostat culture...
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Accounting for Changes in Social Support Among Married Older Adults: Insights From the MacArthur Studies of Successful Aging pptx
... and from their friends and relatives at T2, controlling for T1 levels Prior levels (i.e., 1988 –1989) of negative behaviors accounted for 31% (from spouse), 24% (from children), and 19% (from ... men received the most emotional support from their wives, the women in the sample said they received the most emotional support from their children and from their friends and relatives, at both ... levels of stability with prior levels (i.e., 1988 –1989) accounting for 29% (from spouse), 10% (from children), and 10% (from friends and relatives) of the variance in later (i.e., 1991) levels when...
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báo cáo hóa học:" Mural granulosa cell gene expression associated with oocyte developmental competence" ppt
... down-requlated genes are shown in Table A Euclidean clustering of these differential genes is shown in Fig All four samples from poor oocyte developmental competence (PDC) group had similar gene expression ... (left panel) and mean gene expression intensities (right panel) of detected and undetected genes in gene arrays The number of undetected genes was higher than that of detected genes in all arrays ... immature rat model, gene expression profile in granulosa cells, which may be linked to the developmental competence of the oocyte We hypothesized that expression of specific genes in granulosa...
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Báo cáo hóa học: " Research Article Inferring Time-Varying Network Topologies from Gene Expression Data" docx
... Consider N gene expression profiles, g (1) , g (2) , , g (N) ∈ RT , T being the length of each gene s temporal expression profile (as obtained from microarray expression) The jth time instant of gene ... model to examine the possibility of gene- gene interactions is that gene expression is a function of the underlying cell state and the expression of other genes at the previous time step If longer-range ... particular gene, but only interested in genes from each phase As will be shown in this paper, regime-SSM generates biologically relevant hypotheses regarding time-varying gene interactions during...
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Báo cáo y học: " Dynamic gene network reconstruction from gene expression data in mice after influenza A (H1N1) infection." ppsx
... plant gene expression data BMC Syst Biol 2007, 1:37 Shimamura T, Imoto S, Yamaguchi R, Fujita A, Nagasaki M, Miyano S: Recursive regularization for inferring gene networks from time-course gene expression ... high if gene- gene relationships were considered, but the presented approach is based on cluster centroid expression profiles, which in turn represent the expression trend of sets of genes and ... time-course gene expression data Biostatistics 2007, 8:507-525 Shiraishi Y, Kimura S, Okada M: Inferring cluster-based networks from differently stimulated multiple time-course gene expression...
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Báo cáo y học: "Use of a multi-virus array for the study of human viral and retroviral pathogens: gene expression studies and ChIP-chip analysis" pot
... viral gene expression Analysis of viral gene expression (A) HHV-6B gene expression Only the HHV-6 open reading frames are illustrated and indicated at the bottom of the panel RNA extracted from ... determination of RNA expression levels To demonstrate this capability, we focused on the expression of HHV-6B, HIV-1, and KSHV genes from virally infected cells To analyze HHV-6 gene expression, HHV-6B ... genomic tools Global gene expression arrays, in a single co-hybridization assay, can query the differing expression patterns of thousands of genes [1,2] Differential gene expression can be measured...
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Báo cáo y học: "Threshold-free high-power methods for the ontological analysis of genome-wide gene-expression studies." pptx
... to detect gene categories with varying expression pattern deviations (details in Materials and methods) In short, we simulated the global gene population by drawing 10,000 gene scores from a standard ... 0.1 −5 Gene score 0.8 Nilsson et al R74.3 Population genes Modulated genes Category genes 0.6 0.4 reviews Density distribution 0.3 Volume 8, Issue 5, Article R74 comment 0.4 (b) Population genes ... assessmentgenerating results of the Click oflisting file differential the detectionmethod-method A list here data filethepowers data fromstudies Additionalfor used forpowers expression studiessimulation...
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Báo cáo y học: " Indirect genomic effects on survival from gene expression data" doc
... subsequent text We have gene expression data for both genes from cancer patients and censored survival data from the same patients It is known that expression of PPARD influences expression of ADFP ... other genes by changing their expression level Each interaction was then of the form gene A → gene B, which we write as A → B, representing that the expression of gene A influences the expression ... factors We consider a data set with genes, the expression levels of which are measured using material from patients Note that the data consists of both the gene expression measurements and a data...
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Báo cáo y học: "An annotated cDNA library and microarray for large-scale gene-expression studies in the ant Solenopsis invicta" pot
... genetic mechanism We describe here a collection of 21,715 S invicta ESTs generated from a normalized cDNA library This library should encompass a maximum variety of genes, as it was derived from ... differences, although they develop from the same eggs and are thus genetically identical [1] Lifespan differences must, therefore, stem from differences in gene expression, making ants a useful ... larval and adult gene expression in the ant Camponotus festinatus Evolution 2005, 59:858-870 De Risi JL, Iyer VR, Brown PO: Exploring the metabolic and genetic control of gene expression on a...
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Báo cáo y học: "The proteome of Toxoplasma gondii: integration with the genome provides novel insights into gene expression and annotation" potx
... matching each quartile of genes, according to mRNA expression level There is a general trend for more proteins to have been detected for genes with higher mRNA expression levels (from the top quartile, ... non-redundant release4 genes detected by EST expression from T gondii tachyzoite and bradyzoites (available from ToxoDB) and those detected by this proteome study The number of genes unique to each ... of a single gene, these data represent the expression of 616 non-redundant Toxoplasma genes, of which 547 correspond to release4 gene annotation and 69 are described by alternative gene models...
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Báo cáo khoa học: The fabp4 gene of zebrafish (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression pot
... studying gene expression during vertebrate development, we provide, for the first time in vertebrates, a detailed spatiotemporal expression profile for fabp4 during embryonic and larval development, ... of expression for the fabp4 and fabp3 transcripts during embryonic and larval development in zebrafish (Figs and 6) that suggest distinct function(s) for these two genes during vertebrate development ... fabp4 gene in zebrafish R.-Z Liu et al Fig Zebrafish fabp3 and fabp4 transcripts detected by RT-PCR in RNA extracted from adult tissues RT-PCR products were generated from total RNA extracted from...
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Báo cáo khoa học: Neuropeptide Y expression and function during osteoblast differentiation – insights from transthyretin knockout mice potx
... osteocalcin expression, normalized for the expression of b-actin, at days 3, and 14, (C) osteocalcin expression normalized for the expression of GAPDH and HPRT at day 3, and (D) osteopontin expression, ... into osteoblasts (C) Semiquantitative RT-PCR analysis of PAM expression normalized for b-actin (left) or HPRT (right) expression in BMSCs from WT and TTR KO mice at days and 14 of osteoblast differentiation ... osteocalcin/house keeping gene C 10 b WT TTR KO b HPRT GAPDH Day osteopontin/house keeping gene D 14 b WT 12 peaked on day 7, with significantly increased levels (ranging from two-fold to three-fold)...
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Báo cáo khoa học: Rice cytosine DNA methyltransferases – gene expression profiling during reproductive development and abiotic stress pptx
... Utilizing an in-house generated microarray-based gene expression dataset, expression profiles of all rice MTases were compiled from stages ⁄ tissues of vegetative, panicle and seed development, together ... other genes were observed to be located in unique regions specific to each chromosome (Table 1) Gene expression analysis To gain insight into the developmental windows during which rice MTase genes ... spatial and temporal expression patterns of these genes were analysed during different developmental stages ⁄ tissues For this purpose, an in-house generated microarray expression dataset prepared...
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Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc
... are functional during early development Evidence for developmentally regulated alternative promoter usage in the X laevis MGP gene During early development, X laevis embryos ranging from stages ... usage in this gene during early X laevis development Results Identification of a functional proximal promoter for X laevis Alignment of the 5¢-flanking region of exon IB from the XlMGP gene with the ... transcribed from either maternally inherited mRNAs or developmentally regulated genes, both mechanisms already documented in other genes [27] Similar regulatory mechanisms have been described for genes,...
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báo cáo khoa học: " Global gene expression analysis of apple fruit development from the floral bud to ripe fruit" pot
... R3 Selected genes Figure Clustering of genes changing during fruit development Clustering of genes changing during fruit development Cluster analysis of gene expression A and B, Expression patterns ... levels during fruit development Genes for qRTPCR were initially selected from the list of genes that significantly changed their expression during fruit development The list of regulated genes ... 1955 genes) where gene expression changed significantly during fruit development Hierarchical clustering identified four groups of genes with similar patterns of expression during fruit development...
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Báo cáo y học: "Changes in gene expression during the development of mammary tumors in MMTV-Wnt-1 transgenic mice" ppt
... (b) WntT NeuT Nidogen α-tubulin Figure Gene expression in mammary tumors from MMTV-Wnt-1 versus MMTV-Neu transgenic mice Gene expression in mammary tumors from MMTV-Wnt-1 versus MMTV-Neu transgenic ... [8,20,21] genes in the present study with the 672 genes from the earlier study, we found that 165 genes were present in both lists (Additional data file 1), including 91 of the 178 genes (51%) ... the development of mammary tumors [14] To determine whether unique gene expression patterns accompany the evolution from normal mammary cells to hyperplasias and then to tumors, we compared expression...
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Báo cáo y học: "Molecular processes during fat cell development revealed by gene expression profiling and functional annotation" pptx
... transcription factors expressed during adipogenesis Because knowledge of the transcriptional network during adipogenesis is far from complete, expression profiles have been generated and screened for ... differentiation-specific gene expression during 3T3-L1 adipogenesis Gene 2004, 329:167-185 Jessen BA, Stevens GJ: Expression profiling during adipocyte differentiation of 3T3-L1 fibroblasts Gene 2002, 299:95-100 ... and 22% known genes [18] We then assayed expression The 3T3-L1 cell line treated with a differentiation cocktail was used as a model to study gene expression profiles during adipogenesis Three...
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Gene expression of thyroid hormone receptors TR alpha and TR beta during early development in tilapia, oreochromis mossambicus
... distribution, their expression during early development and the autoinduction of these receptors in the tilapia, O mossambicus In order to understand gene expression of TRs during development in O ... enabled studies on the expression profiles of TRs during development and delineation of the ontogeny of TRα and TRβ (Chapter 3) Localization during early development and differential expression ... understand the effect of thyroid hormones on tilapia development, gene expression of TRα1 and TRβ1 was studied during early development of tilapia from dpf (days post fertilization) until 20 dph (days...
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Báo cáo khoa học: Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags doc
... analysis of gene expression tag sequences into 3¢ complementary DNAs Genes Chromosomes Cancer 33, 252–261 33 van den Berg A, van der Leij J & Poppema S (1999) Serial analysis of gene expression: ... Acids Res 27, e17 34 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression tags for gene identification Proc Natl Acad Sci USA 97, 349–353 ... 3¢ end amplification from SAGE tags W.-J Xu et al extended the widespread use of high-throughput SAGE techniques, and hence accelerated the annotation from tags to genes and genes to genome 12...
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Báo cáo khoa học: Light-induced gene expression of fructose 1,6-bisphosphate aldolase during heterotrophic growth in a cyanobacterium, Synechocystis sp. PCC 6803 ppt
... photoautotrophically with the expression of fbaA, the sll1330 gene is not required for the expression of fbaA under photoautotrophic conditions This means that fbaA gene expression is controlled through ... we assume the decrease in gene expression during growth is due to the lower level of nitrogen in the medium Alternatively, because of the higher concentration of cells during photoautotrophic preculture, ... with respect to the accumulation of inhibitory metabolites [27,28] and gene expression [29] The expression of 10% of the genes was different in the linear growth phase compared to in the logarithmic...
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