sustained gene expression in vitro and in vivo by the ebv replicon vector coupled with hvj liposomes
Báo cáo khoa học: "Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum" pot
... results was reported by other researchers with the direct intraperitoneal injection of L casei 9018 against the sarcoma-180 [19,27] For the antitumor activity of LABs in vivo, the increased specific ... casei and B longum showed high activity on all tumor cells, and led us in vivo antitumor study with these strains Our data showing different antiproliferative activity in these strains consist with ... macrophages and dendritic cells, and NK cells [2932] The CD8+ T cells increased while CD4+ T cells were in marginal change in our experiments This observation is consist with the report with the intraperitoneal...
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báo cáo khoa học: " The impact of bisphosphonates on the osteoblast proliferation and Collagen gene expression in vitro" ppsx
... participated in the design of the study RS and SSY participated in the study design, supported by scientific consulting and coordination and helped to draft the manuscript All authors read and approved the ... zoledronate and clodronate did not significantly influence the gene expression of cyclin D1 during the first days Zoledronate, however, caused a decreasing cyclin D1 gene expression after the 6th ... mineralization The nitrogen-containing bisphosphonates appeared to induce collagen type I gene expression during the first 10 days At day 14 the collagen gene expression level was lowered by the nitrogen...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
... interleukin gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin gene; IFN-ind: the interferon-inducible gene 6-16; IL4R: the interleukin receptor gene; TAP2: the transport ... immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP A1 – the human ... lymphomas In this group of upregulated genes there were the a-myb oncogene, the interleukin receptor gene (IL4R), the gene of the transporter protein TAP2, the gene of protease calpain small subunit...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing docx
... domain is responsible for DNA binding specificity and dimerization, and the ligand-binding domain is responsible for binding of the ligand and associated 1796 induced functions The N-terminal ... splicing of the E2-responsive gene, oxytocin [90] NRs also interact with the SR (serine- and arginine-rich proteins) family of splicing factors CAPER, a SR-like protein, interacts with ERa and the ... and the CpGbinding activity of MLL and its interacting protein CGBP, recruitment of MLLs to a target gene promoter may be in uenced by various other interacting proteins such as Wdr5 and Menin...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... blotting) the concentrations of the caveolin-1 and caveolin-3 proteins in the caveolae of cells stimulated with IL-1b in order to determine whether caveolin synthesis is also modulated by PUFA incorporation ... role in the regulation of cell proliferation, including SMC, by inhibiting signalling molecules [45–47] Caveolin-3 is the main isoform in muscle cells, including SMC [30] Caveolin-1 and caveolin-3 ... threefold) by incubation in medium containing fetal bovine serum In parallel to their effects on the cyclin D1 gene promoter, EPA and DHA also reduced the egr-1 gene promoter activity stimulated by the...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
... After the PBS rinsing, the stain was developed using Fast 3, 3’-diaminobenzidine tablet sets (D4293; Sigma, St Louis, MO) The sections were then counterstained with hematoxylin and analyzed by a ... also increased in mBAT in response to PM2.5 exposure We did not observe significant differences in the gene expressions of Hoxc9 or Igfbp3 in the WAT, neither was the gene expression of Dpt in ... heat instead of ATP synthesis [23,24] As shown in Figure 6, consistent with the fact that PGC-1a induces mitochondrial biogenesis and thermogenesis [25], its gene expression was marked in BAT...
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Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc
... and pUC-KE-tRNACTE-Rz-2, encoding Rz-1 and Rz-2, respectively, were obtained on a multimilligram scale Constructs containing inactive versions of Rz-1 and Rz-2 were obtained in the same way by ... cells were transfected with plasmids encoding Rz-1, Rz-2 and with their inactive versions (Rz-1i and Rz-2i) Transfections of the cells with lipofectin (NO) only or lipofectin and an empty plasmid ... of postincubation the protein fraction was isolated from the cells and subjected to analysis As shown in Fig the extent of protein expression is much lower in the HEK293 cells treated with Rz-1...
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Báo cáo khoa học: Differential gene expression in periportal and perivenous mouse hepatocytes potx
... differences in expression of genes encoding enzymes and other proteins involved in intermediary metabolism Perivenous expression is indicated by green, and genes with preferential periportal expression ... developed the hypothesis that two opposing signaling pathways triggered by Ha-ras- and b-catenin-dependent factors 5058 may determine zonal differences in gene expression in murine liver [22] In addition, ... ornithine transcarbamylase (EC 2.1.3.3), argininosuccinate synthetase (EC 6.3.4.5), argininosuccinate lyase (EC 4.3.2.1), and arginase (EC 3.5.3.1), showing log2 expression ratios between 0.58 and...
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Báo cáo hóa học: " Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA" pot
... ously injected in the neck with 50–100 ug of plasmids dissolved in 100 ul of saline Mice of the control group were subcutaneously injected with saline After h, the suckling mice were challenged with ... sequences without alterations, ensuring the effective activity of the siRNAs described in the present study This work offers an insight into the use of RNAi in animal breeding for disease resistance The ... challenged with and 20 LD50 of the FMDV serotypes O, and Asia I per 0.1 milliliter by subcutaneous injection in the neck near the site that received the injected DNA and were then observed for...
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Báo cáo y học: "Histone deacetylase inhibitors modulate metalloproteinase gene expression in chondrocytes and block cartilage resorption" ppt
... activators in some instances [25] The combination of IL-1 and oncostatin M potently induces both cartilage aggrecan and collagen degradation in vitro and in vivo [41,42] and these factors induce the expression ... or gelatin zymography, as described in Materials and methods potently induced by treatment with IL-1α and OSM and this induction was repressed by both TSA and NaBy in the same manner as the mRNA ... with IL-1α and OSM) Of these induced genes, including ADAMTS5, all but ADAMTS4 showed repression by both TSA and NaBy ADAMTS4, while strongly induced by IL1α and OSM, was not repressed by either...
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Báo cáo y học: "The influence of the NOD Nss1/Idd5 loci on sialadenitis and gene expression in salivary glands of congenic mice" docx
... transforming the intensities, replacing missing values and collapsing probes to genes, the gene lists were filtered by minimum standard deviation = 0.7 Finally, the genes in the list 'pre-processing ... through the endothelial lining and into the SG [33] The reduced gene expression of Ifnz is of interest because the gene lies in the Nss1 congenic region The gene product is known to be inhibitory ... between the strains Gene expression differences of genes located in the NOD loci Idd5 and Nss1 The main focus of the gene expression profiling has been on identifying genes in the two NOD derived...
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Báo cáo y học: "Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration" ppt
... to include the effect of TNF-α on matrix and MMP gene expression in normal and degenerate NP cells as well as the effect of IL-1β and TNF-α on cytokine gene expression and the expression of the ... directly and by gene therapy inhibits matrix degradation in the intact degenerate human intervertebral disc: an in situ zymographic and gene therapy study Arthritis Res Ther 2007, 9:R83 Hoyland JA, ... MMP-9 gene expression in malignant carcinoma via TNF-R1 [25], and TNF-R1 but not TNF-R2 has been shown to be important in inflammatory responses involving IL-1β and MMP-3 and -9 in murine brain injury...
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In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties pps
... Consequently, its increase may be explained by the rise in fibrinogen and/ or globulin concentration and/ or to the modification of the erythrocyte surface properties In our in vitro experiments, ... significantly diminished in the HA group This suggests that incubation with HA introduces changes in the conformation of the cytoskeletal protein spectrin Table 1: Erythrocyte aggregability in the presence ... albumin, could interact with the erythrocyte surface Our hypothesis was that this interaction could lead, in the same way that albumin does, to a reduction in the flexibility of the membrane The...
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Curcumin modulates dopaminergic receptor, CREB and phospholipase c gene expression in the cerebral cortex and cerebellum of streptozotocin induced diabetic rats potx
... receptor binding parameters were determined using Scatchard analysis [32] The specific binding was deter- Page of 11 mined by subtracting non-specific binding from the total The binding parameters, ... to determine the fold change in expression This was done by normalizing the resulting threshold cycle (CT) values of the target mRNAs to the CT values of the internal control β-actin in the same ... activity in diabetic mice, but not in non diabetic mice [46] In our study, curcumin and insulin increased the dopamine D1 receptor expression levels in the cerebellum, which suggests that the curcumin...
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báo cáo khoa học: " Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells" pdf
... various degrees and the mRNA expressions were also increased These results are in accordance with the findings of other reports [15-19], which could be important in the development of new and effective ... Lipfectamine TM2000 according to the protocol (Invitrogen Co.) Cells were harvested and rinsed twice in ice-cold PBS, and kept on ice for 30 in cell lysis buffer containing mM PMSF while agitating ... silencing on gene methylation and mRNA expression of seven tumor suppressor genes in Hela cells assayed by MeDIP combined with Real-Time PCR Except for FHIT and PTEN, the rest five suppressor genes...
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Báo cáo y học: "Comparative effects of the herbal constituent parthenolide (Feverfew) on lipopolysaccharide-induced inflammatory gene expression in murine spleen and liver" ppt
... determine whether differences in expression levels of each gene existed between the spleen and liver These organs contain macrophages and other cell types capable of responding to LPS and other inflammatory ... cytokine production in vitro and in vivo by the herbal constituents apigenin (chamomile), ginsenoside Rb1 (ginseng) and parthenolide (feverfew) Food Chem Toxicol 2005, 41:1381-1390 Kang BY, Chung ... particulate and therefore may be preferentially processed in the spleen rather than liver This may account, at least in part, for the increased cytokine and COX-2 gene expression observed in the spleen...
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Báo cáo y học: "A dynamic model of gene expression in monocytes reveals differences in immediate/early response genes between adult and neonatal cells" potx
... immediately flushed by firmly applying the plunger supplied with the column Elucidating the causes of these defects is a crucial question in neonatal medicine, since infection remains a major cause ... synthesis and secretion is not directly related in mRNA accumulation [29] Thus, gene expression profiling should be complemented with other methods in order to maximize there potential In the final ... identification of the genes within clusters by visual inspection and subsequent functional analysis of genes within clusters (Figures 3A &3B) Figure 3A represents 110 genes of the same cluster...
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Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... biosynthesis may be interrupted by the viral components Cytokines and chemokines generally function in an autocrine (on the producing cell itself) or paracrine (on nearby cells) manner Cytokines ... phase of infection (i.e and hours post-infection), there was an induction of pro-inflammatory cytokines/chemokines At hours post-H5N1/ 2004 infection, there were 2-5 folds increase in the expression ... infected in vitro or in vivo [35-37] The mechanism concerning bronchial infiltration of inflammatory cells, particularly lymphocytes and eosinophils, and the subsequent hyperresponsiveness of the...
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báo cáo khoa học: " Conifer R2R3-MYB transcription factors: sequence analyses and gene expression in wood-forming tissues of white spruce (Picea glauca)" pot
... [18,30,31], including the bHLH-interaction and calmodulin-binding motifs in the DNA-binding domain [28,29], and the repression domain pdLNLD/ELxiG/S in the C-terminus [7] In our study, the spruce ... specific R2R3-MYB gene products in lignin biosynthesis and/ or wood formation is suggested by their expression profiles and by their sequence homology with genes from pine ([10,11]) and in other species ... or two introns, as in Arabidopsis [30] The intron I occurred before the GL amino acid pair in repeat and the intron II occurred after the GN amino acid pair in repeat (Fig 1), as found in the majority...
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báo cáo khoa học: " Impact of AtNHX1, a vacuolar Na+/H+ antiporter, upon gene expression during short- and long-term salt stress in Arabidopsis thaliana" doc
... functions in plant growth and development as well as the stress response (reviewed in [18]) The signaling transcripts with diminished expression in the nhx1 line included a member of the annexin family, ... homeostasis on the proper folding and function of other proteins These include two DnaJ-type genes (At2g20560 and At2g47440), a prefoldin (At1g08780), and a transducin/WD-40 repeat containing gene (At2g22040) ... determine transcripts whose expression levels correlate with the expression of AtNHX1 This approach provides evidence of the influence of a single gene on the expression of other genes while helping...
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