a shift from innate to adaptive response
báo cáo hóa học: " Secretory PLA2-IIA: a new inflammatory factor for Alzheimer''''s disease" pot
... GACTCATGACTGTTGTTACAACC-3' and reverse 5'-TCTCAGGACTCTCTTAGGTACTA-3' that amplify a 493 bp fragment, and primers for β-actin are: forward 5'-TGGAGAAGAGCTATGAGCTGCCTG-3' and reverse 5'-GTGCCACCAGACAGCACTGTGTTG-3' ... phospholipase A2 in reactive astrocytes in response to transient focal cerebral ischemia in the rat brain J Neurochem 2004, 90(3):637-645 Yagami T, Ueda K, Asakura K, Hata S, Kuroda T, Sakaeda T, Takasu ... initiating astrocyte activation and inflammatory cascade [49], its ability to induce sPLA2-IIA mRNA in astrocytes suggests that sPLA2-IIA upregulation could be engaged in early inflammatory events...
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báo cáo khoa học: "Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays" pot
... fluorescence and amplification cycle were generated using the amplified PCR product as a template, and were used to calculate mRNA copy number in each sample Data were expressed as relative mRNA expression ... necessity of DNAse pretreatment and hybridisation Anal Cell Pathol 2001, 22:151-158 Teo IA and Shaunak S Polymerase chain reaction in situ: an appraisal of an emerging technique Histochem J 1995, ... [4] was carried out on a series of total RNA preparations from normal human tissue and paired normal/cancer samples Selection of sequences for oligonucleotide primers was carried out with the aim...
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Phát hiện và phân biệt chủng virus gây hội chứng rối loạn sinh sản và hô hấp ở lợn bằng phương pháp multiplex RT PCR (reverse transcription polymerase chain reaction)
... NA7R ORF7-F ORF7-R PCV2 -A2 PCV2 -A3 CSFV-F CSFV-R Trình tự mồi [5’ – 3’] CAG CCA GTC AAT CAG CTG TG GAA CGT TCG GTC TGG GTG AG ATC CTC CCT GAA TCT GAC AGG TGG GTG GCA GAA AAG CTG TT GTG TCA ATC ... GCA GAA AAG CTG TT GTG TCA ATC AGT GCC ATT GAC C TCC CGT ATT TTC TTG CGC TC GAT GCC ATT TTT CCT TCT CC AAC ATG GAT GGT GTA ACT GG TCT CTA TAG TGT TGG TCA TTC C Ta [oC] 55 55 53 54 Kích thƣớc sản ... HCl; mM EDTA; pH = 8,0 Dung dịch Cân 0,5 g agarose h a tan vào 100 ml dung dịch agarose 0.5% đệm TBE Dung dịch Cân g agarose h a tan vào 100 ml dung dịch đệm agarose % TBE Ethidium Hoà tan g Ethidium...
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Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm a h5n1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
... 46-69 54.6 DiagH5R GACCAAGAACTTTTGGGGATG 416-396 55.4 DiagN1F CCAGTTGGTTGACAATTGGAAT 503-524 54.5 DiagN1R GCATCAGGATAACAGGAGCA 794-775 55.4 Kích thước 154 bp 371 bp 292 bp Tối ưu phản ứng mutiplex ... Swayne DE and Halverson DA (2007), Diseases of Poultry: Influenza, 12th edn Ames, IA: Blackwell Publishing Professional 46 Takano R, Nidom CA, Kiso M, Muramoto Y, Yamada S, Sakai-Tagawa Y, Macken ... K Someya, Y Sakai, T Nguyen, K Nguyen, N Pham, H Nguyen, S Yamada, Y Muramoto, T Horimoto, A Takada, H Goto, T Suzuki, Y Suzuki, and Y Kawaoka (2005), “Avian flu: isolation of drugresistant H5N1...
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Báo cáo khoa học: "TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus" pot
... Probe1 AAACCGGGCCATCAATATGC TGATAAGAGCCAGCACGAATCG 6Fam-TGCCGTGGGCAACGATCCG-Tamra 125-144* 228-249 220-239 5' NTR 125 bp JE2F JE2R Probe2 CCATCACGTACG AATGTCCG GCACCAGCAGTCCACGTCT 6Fam-TGCCGTGGGCAACGATCCGG-Tamra ... 5-3 exonuclease activity of Thermus aquaticus DNA polymerase Proc Natl Acad Sci USA 1991, 88, 72767280 10 Igarashi A, Tanaka M, Morita K, Takasu T, Ahmed A, Ahmed A, Akram DS, Waqar MA Detection ... 6Fam-TGCCGTGGGCAACGATCCGG-Tamra 620-639 666-684 645-664 prM 65 bp JE3F JE3R Probe3 GGTGTA AGGACTAGA GGTTAG AGG ATTCCC AGGTGTCAATATGCTGTT 6Fam-CCCGTGGAAACAACATCATGCGGC-Tamra 10,726-10,750 10,848-10,871...
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Báo cáo khoa học: "Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus" pps
... emaN ecneuqeS eborP esreveR drawroF eborP esreveR drawroF eborP esreveR drawroF '3-pxGCCAATTTCAGCCCAGGCACAAAm-'5 '3-TYCGGTTYGGGACCATGTT-'5 '3-AACTGTACAGRAGGACGTAGA-'5 '3-TAGCGCATGGTGGGCAACCTCCA-'5 ... revo egatnavda eht evah yam yassa siht ,ralucitrap nI selpmas lacinilc ni suriv DMF fo noitceted elbailer dna etarucca ,dipar eht rof elbatius si yassa RCP -TR T/R detamotua eht taht etartsnomed ... ,seniccav dna stset citsongaid rof sdradnats fo launaM seitoozipE seD lanoitanretnI eciffO 17 -36 ,92 ,4002 seneG suriV sniarts aisAnaP rehto dna aeroK ni slamina morf sesuriv esaesid htuom-dna-toof...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... order to determine numerical values Intact mRNA was successfully extracted from as little as 400 µl of whole blood, comparable amounts to that obtained from infants via heelstick This mRNA was used ... different reactions for each sample using increasing amounts of competitor DNA The amounts of sample were quantitated by extrapolating across at least three reactions in which approximately equal amounts ... quantification of GAPDH mRNA by QC-RT-PCR Each value for quantity of CD4 or CD8 mRNA was standardized according to the amount of GAPDH mRNA in the sample Authors' contributions Heather Jaspan...
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Xây dựng qui trình phát hiện đồng thời yeallow head virus(YHV), taura syndrome virus(TSV) và gill associated virus(GAV) trên tôm bằng kỹ thuật multiplex reverse transcription polymerase chain reaction
... 5’-TTGTGAATGTAGCCGTCAGTCGTT-3’ 58,6 YHVr 5’-CCGTTTGAAACTCTCAAAGAAGCC-3’ 56,6 GAVf 5’-ACCGACATCGACATACAAATCCCAATC-3’ 58,8 290bp 496bp GAVr 5’-CCGTCTGTTGTTCGAGAAATTCAACAATC-3’ 58,3 TSVf 5’- TCAGCTTCGACAGTCTCTCCTAATATTG-3’ ... TCAGCTTCGACAGTCTCTCCTAATATTG-3’ 57,6 TSVr 5’-TCAATTATCCAGCAGATGTTCCTGAGG-3’ 58,4 130bp 28 2.1.4 H a chất tách chiết RNA Chúng sử dụng kit tách chiết RNA Công ty Cố Phần Công Nghệ Việt Á ( VA .A9 2-002B ... YHV: Yeallow heat virus TSV: Taura syndrome virus GAV: Gill-associated virus CTV: Cộng tác viên ARN: Acid ribonucleic DNA: Acid deoxyribonucleic PCR: Transcription polymerase chain reaction dNTP:...
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Báo cáo y học: " A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus" pptx
... (5'-ACGTCCTGGACCCTAAGTTTTG-3') was used as a shared reverse primer P5(5'-GGTTTTATAAAAGATT), p6(5'-ATCTAGAGGTAA-3') were used as the primer specific for different CDV vaccine strains Primer pairs ... P1 (5'-AAATCCTGTGTTACCCGCTC-3'), P2 (5'-TGGTGGCTCTGCAATATGAA3'), and P3 (5'-AATGAATGGATGCCTGGGGTTT-3') were used as the primer specific for CDV species, wildtype strains, and vaccine strain Onderstepoort, ... (5'-CCAATTCATCCAAGCTGTCC-3') and P6 (5'-GGGATTTGAACGGTTACATGAG-3') The amplified products were cloned and sequenced, and the sequences were aligned with the H genes of a number of CDV strains available...
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Báo cáo hóa học: "Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real-time reverse transcriptase-polymerase chain reaction to predict recurrence of gastric adenocarcinoma" potx
... Fluorescent and LC-Red probe sequences used for CEA identification were: 5’-CCTGAAATGAAGAAACTACACCAGGGC-fluorescein and 5’-LC-Red 640-GCTATATCAGAGCAACCCCAACCAGCphosphate Real-time PCR monitoring was achieved ... multivariate analyses were used to evaluate factors relating to diseasefree survival According to univariate analysis, age, tumor depth, nodal metastasis, histological grade, TNM stage, CEA mRNA positivity ... Carcinoembryonic antigen Univariate and multivariate analyses of 3-year disease-free survival The 5-year overall survival was 58.9% and 3-year disease free survival was 63.9% Both univariate and multivariate...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
... AGATGTCCAGATTTTA 96 – 111 888F TGTCCAGATCCTCAACAAGTACGA 888 – 911 AATACACTGCACACAGCCAAAGTAG 1005 – 981 TCATGGATTTCCTTCCTGAGCAGCGT 913 – 938 1561F AGTGGTGCTCTGGGTGAGCT 1561 – 1580 1667R TGGTCACGTTCAGGATGAAGG ... GCCTCACACACCGGAGATCT 1130 – 1149 1202R GCTGCACTCACACCAATGGA 1202 – 1183 probe HYAL-3 149 – 127 probe HYAL-2 ACACTGCTGAGGAATGAGATCCA 1005R HYAL-1 CTATGCTTGACCCAGCCTCATC MGB probea HAS-3 TATCCTGCATCAGCGGTCCTCTAGGC ... TGGTCACGTTCAGGATGAAGG 1667 – 1647 probe CCAAGGAATCATGTCAGGCCATCAAGG 1596 – 1622 1069F CGCAGCTGGTGTCATCCTCT 1069 – 1088 1159R CAGCAGCCGTGTCAGGTAATC 1159 – 1139 probe TACACCACAAGCACGGAGACCTGCC 1103...
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Báo cáo y học: " A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses?" docx
... start EEE-F TGTGCGTACCTCCTCATCGTT 335 EEE-R GACTGGCGTGAATCTCTGCTT 414 EEE-Probe HEX364 AGCAGCCTACCTTTCCGACAATGGTTGTCTAMRA WEE-F AGGGATACCCCCGAAGGTT 8220 WEE-R GTGAATAGCACACGGGTGGTT 8322 CTTTCGAATGTCACGTTCCCATGCG ... Nattanmai S, Kramer LD, Bernard KA, Tavakoli NP: A duplex realtime reverse transcriptase polymerase chain reaction assay for the detection of St Louis encephalitis and eastern equine encephalitis ... Pässler et al developed a detecting assay for alphaviruses based on antibodies [14] Lambert et al developed an RT-PCR assay and Taqman RT-PCR assay for WEEV and EEEV [11] But none of the above methods...
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Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx
... China), qualitative duplex real-time RTPCR assay, and COBAS AmpliPrep (CAP)/COBAS TaqMan (CTM) assay (Roche Molecular Systems, Pleasanton, CA) A total of 100 HCV-negative serum samples were obtained ... deviation (SD), as appropriate The intra-assay and inter-assay variations are expressed as SD and coefficient of variation (CV), based on the mean Ct values Probit analysis was performed to determine ... screening and laboratory diagnosis of HCV infection Materials and methods Standards A dilution series of the World Health Organization (WHO) Second International Standard for HCV RNA (National Institute...
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Báo cáo y học: "Multiplex Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) for diagnosis of natural infection with canine distemper virus" potx
... in combination with various templates, namely Vacc-P (lane 1), Vacc-Q (lane 2), a local strain (lane 3), Vacc-P and a local strain (lane 4), Vacc-Q and a local strain (lane 5) and a negative control ... strain amplification were F-vacc: 5'CATCAGCCATGATCAGGGTCTTTTC-3' and R-vacc: 5'-GGGCGGTCTTGTTGGGTATGTGTTT-3' The primers used for field strain amplification were F-wt: 5'AATTCCCAAAAAATCCAAACCCTGC-3' ... incubation at 70°C for 10 Following this, the first round amplification was conducted by polymerase chain reaction (PCR) with the outer primers CDF-F: 5'-AGAGTGCAAAATAGTAAGAATCCAAGC-3' and CDF-R:...
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Báo cáo khoa học: " An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction" pptx
... Table 4: List of clinical signs and symptoms in patients tested Clinical/Laboratory feature n = 124 Number of cases Percentage (%) Pyrexia Arthralgia Headache Ocular pain Emesis Lumbar pain Rash ... observation of clinical and laboratory features, at least 4% of patients had suggestive symptoms This figure gives an average of at least 16 cases of DHF/ DSS per year based on the number of patients ... cells, proteases and lymphokines may be released and activate complement coagulation cascades and vascular permeability factors In 20–30% of DHF cases, the patient develops shock, known as the dengue...
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Báo cáo thú y: " DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls" pot
... TGGACCGGTG 16 AAGACCGGGA ACCCGGTCAC 17 TCCCGGTGAG AACCCGGGAA 18 GAATCCGGCA ACCCGGAAAC TTCCCGGGTT 19 TTTGCCCGGT 20 TGCCGGTTCA CCCGGCATAA CACCCGGATG 21 22 AGCCGGGTAA CCCGGAAGAG TCAGTCCGGG 23 CTACCGGCAC ... that AMP PCR assay could produce DNA marker patterns from genomic and digested Phutikanit et al Acta Veterinaria Scandinavica 2010, 52:18 http://www.actavetscand.com/content/52/1/18 G-S G-L Page ... recognition locations only These particular locations are abundant in the mammalian genome and many may not closely associate with gene regulatory domains To enhance the ability of the AMP PCR in...
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Nghiên cứu xây dựng quy trình phát hiện nhanh virus cúm gia cầm A H5N1 trong mẫu bệnh phẩm bằng kỹ thuật multiplex reverse transcription polymerase chain reacti
... Diethylpyrocarbonate treated water DNA: Deroxi ribonucleic acid dNTP: Deoxyribonucleotide triphosphate EDTA: Etilendiamin tetraaxetic acid HA (H): Haemagglutinin LB: Luria-Bertani media M: Matrix protein NA (N): ... Indonesia, Thái Lan, Việt Nam ph a nam Trung Quốc từ năm 2003 đến [14] Các clade virus cúm A/ H5N1 Các tổ chức WHO (World Health Organization), OIE (Organisation for Animal Health) FAO (Food and Agriculture ... dàng lan truyền quần thể - Hiện tƣợng glycosyl h a: Glycosyl h a (glycosylation) gắn kết chuỗi oligosaccharide với amino acid asparagine số vị trí định 11 chuỗi polypeptide HA hay NA, hay số...
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Recombinant DNA I - Basics of molecular cloning Polymerase chain reaction cDNA clones and screening
... CTTAA G Mix and ligate G AATTC CTTAA G Recombinant molecules G AATTC CTTAA G GAATTC CTTAAG GAATTC CTTAAG Parental molecules DNA ligase covalently joins two DNA molecules • Uses ATP or NADH to provide ... from different sources together with DNA ligase Restriction endonucleases generate ends that facilitate mixing and matching GAATTC CTTAAG GAATTC CTTAAG EcoRI cut G AATTC CTTAA G G AATTC CTTAA ... have a natural system to take up DNA • Treat with inorganic salts to destabilize cell wall and cell membrane • During a brief heat shock, some of the bacteria takes up a plasmid molecule • Can...
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ỨNG DỤNG PHƯƠNG PHÁP PCR (POLYMERASE CHAIN REACTION) VÀ PHƯƠNG PHÁP NUÔI CẤY ĐỂ KHẢO SÁT SỰ NHIỄM VI SINH VẬT GÂY BỆNH TRONG THỰC PHẨM ĐƯỜNG PHỐ
... CTTCACCCGGTTGCCAGAGG E coli Ec2: GGTGATTACCGACGAAAACGG SA1: GCGATTGATGGTGATACGGTT aureus Staphylococcus SA2: AGCCAAGCCTTGACGAACTAAAGC Bacillus cereus BC1: ATTGGTGACACCGATCAAACA BC2: GAATAACATCCATACGTATGA ... BC2: GAATAACATCCATACGTATGA Pl3: AAGTTACCTTTGCTGCATAATCCC perfringens Clostridium Pl7: ATAGATACTCCATATCATCCTGCT Salmonella invA1: TTGTTACGGCTATTTTGACCA invA2: CTGACTGCTACCTTGCTGATG * Quy trình phân ... Tryptic Soy Agar (TSA), Brilliant Green Bile Lactose (BGBL), EMB Agar, Baird Parker Agar (BPA), Rappaport Vassiliadis (RV), Xylose Lysine Desoxycholate (XLD), Simmon Citrate Agar, Brain Heart Infusion...
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KỸ THUẬT PCR (POLYMERASE CHAIN REACTION)
... ACTTTCTACTTCGATGACAGAAGA GGATTATTTGTACAAGATAATGTG ’ Gen GAL4 CCTAATAAACATGTTCTATTACAC 5’ 3’ – CCTAATAAACATGTTCTACCTAGG – 5’ Mồi PCR EcoRI BamHI 5’ – GAATTCATGAAGCTACTGTCTTCT 3’ – CTTAAGTACTTCGATGACAGAAGA ... phản ưng PCR: 5’ – GATCGATCGATACGTGATCC – 3’ 3’ – GCACTAGGATCGATCGATGC – 5’ PCR 5’ – GATCGATCGATACGTGATCCTAGCTAGCTACG – 3’ 3’ – CTAGCTAGCTATGCACTAGGATCGATCGATGC – 5’ * Ghép đôi sai mồi Các mồi oligonucleotit ... GAATTCATGAAGCTACTGTCTTCT 3’ – CTTAAGTACTTCGATGACAGAAGA Gen GAL4 GGATTATTTGTACAAGATAATGTG ’ CCTAATAAACATGTTCTATTACAC ’ Các mồi dùng để nhân phần gen GAL4 từ hệ gen Saccharomyces cerevisiae có gắn thêm vị trí nhận biết...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25