Nghiên cứu đặc điểm dịch tễ và biện pháp phòng trị bệnh đầu đen do đơn bào Histomonas Meleagridis gây ra ở gà tại tỉnh Thái Nguyên

Header Page of 133 I HC THI NGUYấN TRNG I HC NễNG LM TRNG TH XUN NGHIấN CU C IM DCH T V BIN PHP PHềNG TR BNH U EN DO N BO HISTOMONAS MELEAGRIDIS GY RA G TI TNH THI NGUYấN Chuyờn ngnh: Thỳ y Mó s: 62 64 01 01 LUN VN THC S TH Y Ngi hng dn khoa hc: GS TS Nguyn Th Kim Lan Thỏi Nguyờn, nm 2014 S húa bi Trung tõm Hc liu Footer Page of 133 http://www.lrc-tnu.edu.vn/ Header Page of 133 i LI CAM OAN Tụi xin cam oan õy l cụng trỡnh nghiờn cu ca chỳng tụi Cỏc kt qu nghiờn cu lun ny l hon ton trung thc, cha h s dng bo v mt hc v no Mi s giỳp cho vic hon thnh lun u ó c cm n Cỏc thụng tin, ti liu trỡnh by lun ny ó c ghi rừ ngun gc Tỏc gi lun Trng Th Xuõn S húa bi Trung tõm Hc liu Footer Page of 133 http://www.lrc-tnu.edu.vn/ Header Page of 133 ii LI CM N Trong sut nm hc tp, cựng vi s n lc ca bn thõn, tụi ó nhn c s giỳp , hng dn tn tỡnh ca nhiu cỏ nhõn v th, n lun ca tụi ó hon thnh Nhõn dp ny, cho phộp tụi c by t lũng bit n v cm n trõn trng ti cụ giỏo GS TS Nguyn Th Kim Lan v NCS.Ths Trng Th Tớnh ó tn tỡnh hng dn, ch bo v to mi iu kin giỳp tụi sut quỏ trỡnh hon thnh lun tt nghip Tụi xin trõn trng cm n s giỳp to ln v c s vt cht ca Khoa Chn nuụi thỳ y - Trng i hc Nụng Lõm Thỏi Nguyờn v Khoa Nụng Lõm - Trng Cao ng Kinh t k thut Thỏi Nguyờn Tụi xin by t lũng cm n chõn thnh ti Ban giỏm hiu, Phũng QLT Sau i hc Trng i hc Nụng Lõm Thỏi Nguyờn ó giỳp to iu kin cho tụi sut quỏ trỡnh hc v nghiờn cu ti trng Tụi xin trõn trng cm n s giỳp nhit tỡnh ca cỏn b Trm thỳ y v nhõn dõn ca cỏc huyn Phỳ Bỡnh, Ph Yờn, Vừ Nhai, nh Húa (tnh Thỏi Nguyờn) ó to iu kin giỳp tụi sut quỏ trỡnh thc hin ti Cui cựng tụi xin cm n s ng viờn khớch l ca gia ỡnh, bn bố v ng nghip sut thi gian thc hin lun khoa hc ny Tụi xin trõn trng cm n! Thỏi Nguyờn, ngy thỏng nm 2014 Tỏc gi lun Trng Th Xuõn S húa bi Trung tõm Hc liu Footer Page of 133 http://www.lrc-tnu.edu.vn/ Header Page of 133 iii MC LC LI CAM OAN i LI CM N ii MC LC iii DANH MC CH VIT TT v DANH MC CC BNG vi DANH MC CC HèNH vii M U 1 Tớnh cp thit ca ti Mc tiờu nghiờn cu í ngha khoa hc v ý ngha thc tin 3.1 í ngha khoa hc 3.2 í ngha thc tin Nhng úng gúp mi ca ti Chng 1:TNG QUAN TI LIU .3 1.1 C s khoa hc ca ti 1.1.1 c im ca n bo H meleagridis ký sinh gia cm 1.1.2 Bnh u en g (Histomonosis) 10 1.2 Tỡnh hỡnh nghiờn cu v ngoi nc 20 1.2.1 Tỡnh hỡnh nghiờn cu nc 20 1.2.2 Tỡnh hỡnh nghiờn cu trờn th gii 22 Chng 2: I TNG, VT LIU, NI DUNG V PHNG PHP NGHIấN CU 33 2.1 i tng v vt liu nghiờn cu 33 2.1.1 i tng nghiờn cu 33 2.1.2 Vt liu nghiờn cu 33 2.2 a im, thi gian nghiờn cu 33 2.2.1 a im nghiờn cu .33 2.2.2 Thi gian nghiờn cu 34 2.3 Ni dung nghiờn cu 34 2.3.1 Nghiờn cu c im dch t bnh u en g ti Thỏi Nguyờn 34 2.3.2 Nghiờn cu bnh u en H meleagridis gõy g .34 S húa bi Trung tõm Hc liu Footer Page of 133 http://www.lrc-tnu.edu.vn/ Header Page of 133 iv 2.3.3 Nghiờn cu phỏc iu tr bnh u en cho g 34 2.4 Phng phỏp nghiờn cu .34 2.4.1 Phng phỏp nghiờn cu c im dch t bnh u en H meleagridis gõy g nuụi ti Thỏi Nguyờn 34 2.4.2 Phng phỏp nghiờn cu bnh u en n bo H meleagridis gõy g .39 2.4.3 Phng phỏp nghiờn cu bin phỏp phũng tr bnh u en cho g 39 2.5 Phng phỏp x lý s liu .41 Chng 3: KT QU NGHIấN CU 42 3.1 c im dch t bnh n bo H meleagridis gõy g ti Thỏi Nguyờn 42 3.1.1 Kt qu iu tra thc trng phũng chng bnh ký sinh trựng núi chung cho g tnh Thỏi Nguyờn .42 3.1.2 Tỡnh hỡnh nhim H meleagridis g ti tnh Thỏi Nguyờn 43 3.1.3 Nghiờn cu s liờn quan gia bnh u en v bnh giun kim g 53 3.2 Nghiờn cu bnh u en H meleagridis gõy g ti Thỏi Nguyờn 59 3.2.1 Triu chng lõm sng ca g b bnh u en ti tnh Thỏi Nguyờn 59 3.2.2 Bnh tớch ca g b bnh u en cỏc a phng .60 3.3 Nghiờn cu bin phỏp phũng chng bnh u en cho g 63 3.3.1 Th nghim hiu lc ca mt s thuc iu tr bnh u en .63 3.3.2 xut bin phỏp phũng chng bnh u en cho g 66 KT LUN V NGH .68 Kt lun .68 ngh 69 TI LIU THAM KHO 70 S húa bi Trung tõm Hc liu Footer Page of 133 http://www.lrc-tnu.edu.vn/ Header Page of 133 v DANH MC CH VIT TT cm : Centimet cs : Cng s E coli : Escherichia coli E tenella : Eimeria tenella g : Gam kg : Kilogam Nxb : Nh xut bn pp : Plural page spp : species pluralis tr : Trang m : Micromet S húa bi Trung tõm Hc liu Footer Page of 133 http://www.lrc-tnu.edu.vn/ Header Page of 133 vi DANH MC CC BNG Bng 3.1 Thc trng phũng chng bnh ký sinh trựng cho g tnh Thỏi Nguyờn 42 Bng 3.2: T l nhim n bo H meleagridis g theo a phng .44 Bng 3.3 T l nhim H meleagridis g theo la tui 46 Bng 3.4 T l nhim H meleagridis g theo phng thc chn nuụi 49 Bng 3.5 T l nhim H meleagridis g theo tỡnh trng v sinh thỳ y 51 Bng 3.6 T l v cng nhim giun kim g m khỏm 53 Bng 3.7 T l nhim H meleagridis s g nhim giun kim 56 Bng 3.8 T l nhim H meleagridis s g khụng nhim giun kim 58 Bng 3.9 T l v cỏc triu chng lõm sng ca g mc bnh u en 59 Bng 3.10 Bnh tớch i th ca g b bnh u en Thỏi Nguyờn 60 Bng 3.11 Bnh tớch vi th ca g mc bnh u en .62 Bng 3.12 Th nghim ca thuc tr bnh u en cho g trờn din hp 63 Bng 3.13 Th nghim ca thuc tr bnh u en cho g trờn din rng 65 S húa bi Trung tõm Hc liu Footer Page of 133 http://www.lrc-tnu.edu.vn/ Header Page of 133 vii DANH MC CC HèNH Hỡnh 3.1 Biu t l nhim H meleagridis g ti mt s huyn thuc tnh Thỏi Nguyờn .45 Hỡnh 3.2 th t l nhim H meleagridis theo tui g 48 Hỡnh 3.3 Biu t l nhim H meleagridis theo phng thc chn nuụi g .50 Hỡnh 3.4 Biu t l nhim H meleagridis theo tỡnh trng v sinh thỳ y .53 Hỡnh 3.5 Biu t l nhim giun kim g v t l nhim H meleagridis s g nhim giun kim 57 S húa bi Trung tõm Hc liu Footer Page of 133 http://www.lrc-tnu.edu.vn/ Header Page of 133 M U Tớnh cp thit ca ti Ngnh chn nuụi nc ta ó v ang chim mt v trớ quan trng sn xut nụng nghip núi riờng v c cu nn kinh t núi chung Chn nuụi vi nhiu phng thc phong phỳ v a dng ó gúp phn gii quyt vic lm, nõng cao thu nhp cho ngi dõn, to cỏc sn phm cú giỏ tr cao, phự hp vi th hiu ngi tiờu dựng Theo s liu ca Tng Cc thng kờ, tng n gia cm ti thi im 01/4/2013 l 314,4 triu con, tng 1,2% so vi nm 2012 Trong ú, chn nuụi g th khong 80 % Vit Nam l nc nm vựng khớ hu nhit i núng m cú khu h ký sinh trựng phong phỳ vi nhiu ging, loi ký sinh gõy bnh cho gia sỳc, gia cm Thỏi Nguyờn l mt tnh nỳi, cú nhiu a phng ang phỏt trin mnh ngh chn nuụi g Tuy nhiờn, quỏn chn nuụi g nh l, tn dng ngun thc n t nhiờn nờn to iu kin thun li cho cỏc bnh ký sinh trựng núi chung v bnh giun kim g núi riờng phỏt trin, kộo theo s phỏt trin ca bnh n bo Histomonas meleagridis gõy Bnh n bo Histomonas meleagridis (bnh u en) g hin ó xut hin nhiu a phng, c bit l cỏc a phng cú ngnh chn nuụi g v g tõy theo li trung cụng nghip Bnh gõy tỏc hi ỏng k i vi chn nuụi g ti cỏc a phng v lm gim hiu qu kinh t chn nuụi Bnh u en l mt bnh ký sinh trựng nguy him g v g tõy n bo Histomonas meleagridis gõy Bnh gõy nhng biu hin bt thng da vựng u, ban u cú mu xanh tớm, sau ú nhanh chúng tr lờn thõm en nờn cú tờn l bnh u en Bnh cú nhng bnh tớch c trng nh: viờm hoi t to m rut tha v gan, th trng xu, da vựng u v mo tớch thõm en G bnh cht ri rỏc v thng cht v ban ờm, mc cht khụng t nhng hin tng cht kộo di, gõy cho ngi chn nuụi cm giỏc bnh khụng nguy him lm, thc cht cui cựng g cht n 85 - 95 % S húa bi Trung tõm Hc liu Footer Page of 133 http://www.lrc-tnu.edu.vn/ Header Page 10 of 133 Mc dự vy, Vit Nam cha cú cụng trỡnh nghiờn cu no v bnh u en g, vỡ vy cng cha cú quy trỡnh phũng chng bnh cho g hiu qu Thỏi Nguyờn l mt nhng tnh chn nuụi nhiu g, c bit l g th Trong nm gn õy nhiu a phng ca tnh Thỏi Nguyờn ó liờn tc xut hin bnh u en trờn n g, bnh cú t l cht cao gõy thit hi ln cho ngi chn nuụi Xut phỏt t yờu cu cp thit ca vic khng ch dch bnh nhm nõng cao nng xut chn nuụi g, chỳng tụi thc hin ti: Nghiờn cu c im dch t v bin phỏp phũng tr bnh u en n bo Histomonas meleagridis gõy g ti tnh Thỏi Nguyờn Mc tiờu nghiờn cu - Nghiờn cu c im dch t bnh u en n bo Histomonas meleagridis g nuụi ti tnh Thỏi Nguyờn - Nghiờn cu c im bnh u en n bo Histomonas meleagridis gõy g - Nghiờn cu bin phỏp phũng tr v xut quy trỡnh phũng tr bnh u en cho g í ngha khoa hc v ý ngha thc tin 3.1 í ngha khoa hc Kt qu ca ti l nhng thụng tin khoa hc v c im dch t, v bnh hc v quy trỡnh phũng chng bnh u en cho g, cú mt s úng gúp mi cho khoa hc 3.2 í ngha thc tin Kt qu ca ti l c s khoa hc khuyn cỏo ngi chn nuụi ỏp dng quy trỡnh phũng, tr bnh u en cho g nhm hn ch t l nhim v thit hi bnh u en gõy ra; gúp phn nõng cao nng sut chn nuụi, thỳc y ngnh chn nuụi phỏt trin Nhng úng gúp mi ca ti - L cụng trỡnh nghiờn cu tng i h thng v c im dch t, bnh hc v bin phỏp phũng tr bnh u en n bo Histomonas meleagridis gõy g - Xõy dng quy trỡnh phũng, tr bnh u en n bo Histomonas meleagridis gõy g cú hiu qu, khuyn cỏo v ỏp dng rng rói ti cỏc nụng h, cỏc trang tri chn nuụi g S húa bi Trung tõm Hc liu Footer Page 10 of 133 http://www.lrc-tnu.edu.vn/ Header Page 75 of 133 67 - Thuc tng cng sc khỏng cho g: in gii, B - complex Ngoi phi phũng v iu tr bnh giun kim v bnh cu trựng g S húa bi Trung tõm Hc liu Footer Page 75 of 133 http://www.lrc-tnu.edu.vn/ Header Page 76 of 133 68 KT LUN V NGH Kt lun 1.1 c im dch t bnh n bo Histomonas meleagridis g ti Thỏi Nguyờn - T l nhim Histomonas meleagridis g ti huyn: Phỳ Bỡnh, Ph Yờn, Vừ Nhai, nh Húa l 13,45 %, ú huyn Phỳ Bỡnh cú t l g nhim bnh cao nht (32,86 %) v thp nht huyn nh Húa (4,29 %) - G cỏc la tui u nhim n bo Histomonas meleagridis T l nhim n bo Histomonas meleagridis nht g t thỏng tui (27,96%), sau ú gim dn - G nuụi theo phng thc chn th hon ton v bỏn chn th cú t l nhim n bo Histomonas meleagridis cao hn so vi phng thc nuụi nht (20,37 % v 13,24 %, so vi 5,65%) - Tỡnh trng v sinh thỳ y nh hng rừ rt n t l nhim Histomonas meleagridis g V sinh thỳ y tt, t l nhim n bo g ch l 5,65 %, v sinh thỳ y kộm thỡ t l ny cao (24,62 %) - Giun kim Heterakis gallinarum l ký ch trung gian ca n bo Histomonas meleagridis: + T l nhim giun kim g m khỏm ti Thỏi Nguyờn l 32,17 %, ch yu g nhim giun kim mc nng v trung bỡnh + T l nhim H meleagridis g t l thun vi t l nhim giun kim 1.2 Nghiờn cu bnh u en H meleagridis gõy g ti Thỏi Nguyờn Triu chng lõm sng ch yu ca g mc bnh u en l: r, lụng xự, ng run ry, mt nhm, st cao 43oC 44oC, ung nhiu nc, gim n hoc b n, tiờu chy, phõn loóng mu hng hoc ln mỏu, mo tớch nht nht hoc tỏi xanh, giai on cui g tiờu chy, phõn loóng mu vng lu hunh c trng ca bnh T l cỏc triu chng bin ng t 56,45% n 100% - Bnh tớch i th ca g mc bnh u en ch yu trung gan v manh trng: mt hoc c hai manh trng sng to, úng kộn trng Gan sng to, cú nhiu nt hoi t lừm nh ỏ hoa cng T l cỏc bnh tớch i th bin ng t 10,62% n 78,76% S húa bi Trung tõm Hc liu Footer Page 76 of 133 http://www.lrc-tnu.edu.vn/ Header Page 77 of 133 69 - Bnh tớch vi th gan v manh trng: T bo gan b hoi t, thoỏi húa hoc thõm nhim nhiu t bo viờm; niờm mc manh trng b phỏ hy 100% mu bnh phm gan v manh trng cú bin i vi th rừ rt 1.3 Bin phỏp phũng chng bnh u en cho g Phỏc I: gm TD - daminton, Hancillin-50, paravet, hepatol, Hanlyte vit C v phỏc II: gm T coryzin, Norfacoli, paravet, hepatol, Hanlyte vit C u cho kt qu tt (80,00% 86,67 % din hp v 92% 94% din rng) ngh Qua kt qu nghiờn cu ca ti, chỳng tụi thy, g nuụi ti tnh Thỏi Nguyờn mc bnh u en khỏ nhiu Vỡ vy, chỳng tụi cú ngh sau: Cỏc h chn nuụi g cn thc hin ng b cỏc bin phỏp phũng bnh cho g núi chung v phũng bnh u en cho g núi riờng: thc hin tt cụng tỏc v sinh chung tri, thc n, dng c chn nuụi, s dng vc xin phũng bnh cho g theo ỳng quy trỡnh chn nuụi, nh k ty giun, sỏn phũng cỏc bnh ký sinh trựng cho g, tng cng cụng tỏc chm súc, nuụi dng nõng cao sc khỏng ca n g iu tr bnh u en cho g bng thuc TD - daminton hoc T coryzin kt hp vi thuc iu tr triu chng v thuc tr sc tr lc S húa bi Trung tõm Hc liu Footer Page 77 of 133 http://www.lrc-tnu.edu.vn/ Header Page 78 of 133 70 TI LIU THAM KHO I Ti liu ting Vit Nguyn Xuõn Bỡnh, Trn Xuõn Hnh, Tụ Th Phn (2002), 109 bnh gia cm v cỏch phũng tr, Nxb Nụng nghip, H Ni Phm Vn Khuờ, Phan Lc (1996), Ký sinh trựng thỳ y, Nxb Nụng nghip, H Ni, tr 130 - 133, 138 - 140 Nguyn Th Kim Lan, Nguyn Vn Quang, Nguyn Quang Tuyờn (1999), Giỏo trỡnh ký sinh trựng thỳ y, Nxb Nụng nghip, H Ni, tr 101 104, 107 - 108 Nguyn Th Kim Lan, Nguyn Th Lờ, Phm S Lng, Nguyn Vn Quang (2008), Ký sinh trựng hc thỳ y (giỏo trỡnh dựng cho bc cao hc), Nxb Nụng nghip, H Ni, tr 72 - 78 Phm S Lng, Phan ch Lõn (2001), Bnh ký sinh trựng gia cm, Nxb Nụng nghip, H Ni Phm S Lng, Tụ Long Thnh (2006), B ý sinh vt nuụi, Nxb Nụng nghip, H Ni, tr 92 - 95 Nguyn Th Lờ, Nguyn Th K, Phm Vn Lc, H Duy Ng, Nguyn Th Minh(1996), Giun sỏn ký sinh gia cm Vit Nam, Nxb Khoa hc v K thut, H Ni, tr 125 - 162 Phan Lc (2006), Giỏo trỡnh bnh ký sinh trựng thỳ y (dựng cỏc trng trung hc chuyờn nghip), Nxb H Ni, tr 129 130 Lờ Vn Nm (2010), Bnh viờm Gan - Rut truyn nhim, Tp khoa hc k thut thỳ y, II, s 10 Lờ Vn Nm (2011), Bnh u en g v g tõy, Tp khoa hc cụng ngh chn nuụi, tr 88 - 91 11 Nguyn Vn Thin (2008), Thng kờ sinh vt hc ng dng chn nuụi, Nxb Nụng Nghip, H Ni S húa bi Trung tõm Hc liu Footer Page 78 of 133 http://www.lrc-tnu.edu.vn/ Header Page 79 of 133 71 II Ti liu ting Anh 12 Alkhalaf A N and Mahmoud O M (2009), An outbreak of concurrent Histomonas meleagridis and Enteroccocus fecalis infection in ducks, Asian J of Poultry Sci., 3: 15 - 18 13 Armstrong P L., McDougald L R (2011), The infection of turkeys with Histomonas meleagridis caused by exposure to infected poultry or contaminated cages, Source Department Poultry Science, University of Georgia, Athens, GA 30602, USA 14 Banerjee P S and Yadav C L, (2002), Infectious enterohepatitis in chickens in Uttar Pradesh, Indian, J Anim Sci., 71:30-31 15 Bleyen N., De Gussem K., Pham A D., Ons E., Van Gerven N., Goddeeris B M (2009), Non - curative, but prophylactic effects of paromomycin in Histomonas meleagridis - infected turkeys and its effect on performance in non-infected turkeys, Vet Parasitol., 165 (3 - 4): 248 - 255 16 Callait-Cardinal M P., Leroux S., Venereau E., Chauve C M., Le Pottier G and Zenner L (2007), Incidence of histomonosis in turkeys in France since the bans of dimetridazole and nifursol, Vet Rec., 161: 581 - 585 17 Callait-Cardinal M P., Gilot-Fromont E., Chossat L., Gonthier A., Chauve C., Zenner L (2010), Flock management and histomoniasis in free-range turkeys in France: description and search for potential risk factors, Epidemiol Infect., 138(3): 353 - 363 18 Cepicka I., Hamp V and Kulda J (2010), Critical Taxonomic Revision of Parabasalids with Description of one new Genus and three new Species, Protist, 161: 400 - 433 19 Chute M B., Chute A M., Wilkins G C (1978), Effect of dimetridazole on transmission of Histomonas meleagridis by Heterakis gallinarum, Parasitology, 77(1): 41- 20 Conraths F J., Werner O., Methner U., Geue L., Schulze F., Họnel I., Sachse K., Hotzel H., Schubert E., Melzer F., Mettenleiter T C (2005), Conventional and alternative housing systems for poultry point of view of infectious disease medicine, 118(5-6):186-204 S húa bi Trung tõm Hc liu Footer Page 79 of 133 http://www.lrc-tnu.edu.vn/ Header Page 80 of 133 72 21 De Gussem M., De Gussem J (2006), Proceedings of the 6th International Symposium on turkey diseases, Berlin, pp 210 - 218 22 Farr M (1961), Further observations on survival of the protozoan parasite Histomonas meleagridis and eggs of poultry nematodes in the feces of infected birds, Cornell Vet., 51: 23 Ganas P., Liebhart D., Glửsmann M., Hess C and Hess M (2012), Escherichia coli strongly supports the growth of Histomonas meleagridis, in a monoxenic culture, without influence on its pathogenicity, J Parasitol., 42 (10): 893 - 901 24 Gibbs B J (1962), The occurrence of the protozoa parasite Histomonas meleagridis in the adults and eggs of the cecal worm Heterakis gallinae, J Protozool., 9: 288 - 293 25 Graybill H W and Smith T (1920), Production of fatal blackhead in turkeys by feeding embryonated eggs of Heterakis papillosa, J Exp Med, 31: 647 - 655 26 Gregory V., Lamann (2010), Veterinary parasitology, Nova Biomedical Press, Inc, 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Ghaffar F., Rasheid K A., Grabensteiner E., Hess M (2010), Experiments to produce cysts in cultures of Histomonas meleagridis - the agent of histomonosis in poultry, Parasitol Res., 106 (4): 1005 - 1007 Ti liu ting Sec 69 Jana Choutkovỏ (2010), Význam hlớstic pro penos parazitických prvokna novộ hostitele, Pớrodovdeckỏ fakulta Univerzity Karlovy v Praze Katedra parazitologie, pp - 30 S húa bi Trung tõm Hc liu Footer Page 84 of 133 http://www.lrc-tnu.edu.vn/ Header Page 85 of 133 MT S HèNH NH MINH HA CHO TI Hỡnh 1: G nuụi chn th b bnh u en Hỡnh 2: G nuụi bỏn chn th b bnh u en Hỡnh 3: G nuụi nht b bnh u en Hỡnh 4: G b bnh u en mo tớm tỏi S húa bi Trung tõm Hc liu Footer Page 85 of 133 Hỡnh 5: G bnh r, nm bp nghiờng v mt bờn http://www.lrc-tnu.edu.vn/ Header Page 86 of 133 Hỡnh 6: Phõn g b bnh u en tiờu chy,mu vng lu hunh Hỡnh 8: M khỏm kim tra bnh tớch g ti phũng thớ nghim Hỡnh 10: G b bnh u en manh trng cú nhiu giun kim ký sinh S húa bi Trung tõm Hc liu Footer Page 86 of 133 Hỡnh : M khỏm kim tra bnh tớch g ti hin trng Hỡnh 9: M khỏm g ngy 19/10/2013 Hỡnh 11: Thu thp giun kim ký sinh g b bnh u en http://www.lrc-tnu.edu.vn/ Header Page 87 of 133 Hỡnh 12: Giun kim g i kớnh hin vi (x 40) Hỡnh 13: Trng giun kim di kớnh hin vi (x100) Hỡnh 14: Mu gan v manh trng ca g m khỏm ngy 22/10/2013 Hỡnh 15: Bnh tớch gan v manh trng ca g m khỏm Hỡnh 16: hoi t lừm hỡnh hoa cỳc trờn b mt gan S húa bi Trung tõm Hc liu Footer Page 87 of 133 Hỡnh 17: hoi t thựy gan http://www.lrc-tnu.edu.vn/ Header Page 88 of 133 Hỡnh 18: Bnh tớch gan v manh trng ca g b bnh u en Hỡnh 19: Manh trng ca g b bnh u en phỡnh to, dy lờn v cú mu vng xỏm Hỡnh 20: n bo H meleagridis xõm nhp t chc gan (Tiờu bn nhum HE, phúng i 400 ln) Hỡnh 21: Mụ gan b thoỏi húa, xut huyt Hỡnh 22: n bo H meleagridis lp h niờm mc ca manh trng (Tiờu bn nhum HE X 150) Hỡnh 23.:n bo H meleagridis hoi t gan (Tiờu bn nhum HE X 600) S húa bi Trung tõm Hc liu Footer Page 88 of 133 (Tiờu bn nhum HE X 200) http://www.lrc-tnu.edu.vn/ Header Page 89 of 133 Hỡnh 25: Phỏc iu tr bnh Hỡnh 26: Phỏc iu tr bnh u en cho g u en cho g S húa bi Trung tõm Hc liu Footer Page 89 of 133 http://www.lrc-tnu.edu.vn/ ... Nghiên cứu đặc điểm dịch tễ biện pháp phòng trị bệnh đầu đen đơn bào Histomonas meleagridis gây gà tỉnh Thái Nguyên Mục tiêu nghiên cứu - Nghiên cứu đặc điểm dịch tễ bệnh đầu đen đơn bào Histomonas. .. Histomonas meleagridis gà nuôi tỉnh Thái Nguyên - Nghiên cứu đặc điểm bệnh đầu đen đơn bào Histomonas meleagridis gây gà - Nghiên cứu biện pháp phòng trị đề xuất quy trình phòng trị bệnh đầu đen cho gà. .. trình nghiên cứu tương đối hệ thống đặc điểm dịch tễ, bệnh học biện pháp phòng trị bệnh đầu đen đơn bào Histomonas meleagridis gây gà - Xây dựng quy trình phòng, trị bệnh đầu đen đơn bào Histomonas