METHODS IN ENZYMOLOGY pdf

[...]... protein factors, and cellular processes that mediate and/or regulate RNA turnover RNA elements, including primary sequences and, frequently, higher-order structures, can influence the decay functions of a variety of protein factors such as exoribonucleases, endoribonucleases, helicases, and other types of RNA-binding proteins, all of which often exist in multiprotein complexes Cellular processes having... Preface include transcription, translation, and polyadenylation Many experimental approaches for analyzing RNA decay are presented, each generally focusing on a specific organism and a particular class of RNA such as mRNA, a stable RNA, or a type of small noncoding RNA Techniques presented include isolating total-cell or organelle RNA by different methods, determining RNA half-lives, establishing in vitro... by VICTOR GINSBURG Methods in Enzymology xxxiii VOLUME 139 Cellular Regulators (Part A: Calcium- and Calmodulin-Binding Proteins) Edited by ANTHONY R MEANS AND P MICHAEL CONN VOLUME 140 Cumulative Subject Index Volumes 102–119, 121–134 VOLUME 141 Cellular Regulators (Part B: Calcium and Lipids) Edited by P MICHAEL CONN AND ANTHONY R MEANS VOLUME 142 Metabolism of Aromatic Amino Acids and Amines Edited... Protein Phosphorylation (Part A: Protein Kinases: Assays, Purification, Antibodies, Functional Analysis, Cloning, and Expression) Edited by TONY HUNTER AND BARTHOLOMEW M SEFTON VOLUME 201 Protein Phosphorylation (Part B: Analysis of Protein Phosphorylation, Protein Kinase Inhibitors, and Protein Phosphatases) Edited by TONY HUNTER AND BARTHOLOMEW M SEFTON VOLUME 202 Molecular Design and Modeling: Concepts... CARTER, JR., AND ROBERT W SWEET VOLUME 375 Chromatin and Chromatin Remodeling Enzymes (Part A) Edited by C DAVID ALLIS AND CARL WU VOLUME 376 Chromatin and Chromatin Remodeling Enzymes (Part B) Edited by C DAVID ALLIS AND CARL WU VOLUME 377 Chromatin and Chromatin Remodeling Enzymes (Part C) Edited by C DAVID ALLIS AND CARL WU VOLUME 378 Quinones and Quinone Enzymes (Part A) Edited by HELMUT SIES AND... Protein Aggregates Edited by RONALD WETZEL VOLUME 310 Biofilms Edited by RON J DOYLE VOLUME 311 Sphingolipid Metabolism and Cell Signaling (Part A) Edited by ALFRED H MERRILL, JR., AND YUSUF A HANNUN Methods in Enzymology xliii VOLUME 312 Sphingolipid Metabolism and Cell Signaling (Part B) Edited by ALFRED H MERRILL, JR., AND YUSUF A HANNUN VOLUME 313 Antisense Technology (Part A: General Methods, Methods. .. Hybrid Proteins (Part B: Cell Biology and Physiology) Edited by JEREMY THORNER, SCOTT D EMR, AND JOHN N ABELSON VOLUME 328 Applications of Chimeric Genes and Hybrid Proteins (Part C: Protein–Protein Interactions and Genomics) Edited by JEREMY THORNER, SCOTT D EMR, AND JOHN N ABELSON xliv Methods in Enzymology VOLUME 329 Regulators and Effectors of Small GTPases (Part E: GTPases Involved in Vesicular... Protein Pathways (Part C: Effector Mechanisms) Edited by RAVI IYENGAR AND JOHN D HILDEBRANDT Methods in Enzymology xlv VOLUME 346 Gene Therapy Methods Edited by M IAN PHILLIPS VOLUME 347 Protein Sensors and Reactive Oxygen Species (Part A: Selenoproteins and Thioredoxin) Edited by HELMUT SIES AND LESTER PACKER VOLUME 348 Protein Sensors and Reactive Oxygen Species (Part B: Thiol Enzymes and Proteins)... VOGT AND INDER M VERMA VOLUME 255 Small GTPases and Their Regulators (Part A: Ras Family) Edited by W E BALCH, CHANNING J DER, AND ALAN HALL VOLUME 256 Small GTPases and Their Regulators (Part B: Rho Family) Edited by W E BALCH, CHANNING J DER, AND ALAN HALL xl Methods in Enzymology VOLUME 257 Small GTPases and Their Regulators (Part C: Proteins Involved in Transport) Edited by W E BALCH, CHANNING J DER,... VOLUME 381 Oxygen Sensing Edited by CHANDAN K SEN AND GREGG L SEMENZA Methods in Enzymology xlvii VOLUME 382 Quinones and Quinone Enzymes (Part B) Edited by HELMUT SIES AND LESTER PACKER VOLUME 383 Numerical Computer Methods (Part D) Edited by LUDWIG BRAND AND MICHAEL L JOHNSON VOLUME 384 Numerical Computer Methods (Part E) Edited by LUDWIG BRAND AND MICHAEL L JOHNSON VOLUME 385 Imaging in Biological Research . include isolating total-cell or organelle RNA by different methods, determining RNA half-lives, establishing in vitro and in vivo RNA degradation assays, mapping full-length RNAs and decay intermediates,. or transmitted in any form orby any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the Publisher. The. made toward explaining cleavage mechanisms and how such mechanisms are regulated. In view of research developments over the past 20 years, it is surprising that a Methods in Enzymology on RNA