Hoàn thiện quy trình ly trích hoạt chất limonoid trên quy mô pilot từ cây neem và điều chế các phụ gia thích hợp để làm nguyên liệu pha chế thuốc bảo vệ thực vật

UY BAN NHAN DAN TP.HCM SCA KHOA HOC VA LONG NGHe oa.:qo TRUNG TAM NC OA SINH ING DUNG BAO CAO NGHIEM THU De tai HOAN THICN QUY TRiNH TRICH LY HOAT CHAT LIMONOID TREN QUY MO PILOT TU CAY NEEM VA DIEU CHE CAC PHV GIA THICH HOP DE LAM NGUYEN LIN PHA CHE THUOC BAO VE THVC V41GSTS TrAn Kim Qui THANH 13116 116 CHI MINH THANG 10 NAM 2006 THAN, HOA, QUA VA LA CAY NEEM TEN KHOA HQC : AZADIRACHTA INDICA BAO CAO NGHIEM THU DE TAI Ten d tai : Hoan thien quy trinh ly trich hoat chdt limonoid tren quy m6 pilot ta' cay neem va Eau the cac phu gia thich hdp de lam nguyen lieu pha the thu6c tra' sau bao ye thuc vat Chti nhiem de tai : GSTS Tr'dn Kim Qui Cd quan chi tri : Trung tam nghien eau flOa Sinh ang dung Thai gian thurc hien d tai : tit thang 08/2004 den 02/2006 Kinh phi dtigc duyet : 161.000.000 d Kinh phi 65 cap :145.000.000d theo TB s6 : 137/TB-SKHCN 9/8/2004 va TB s6: 225/TB-SKHCN 18/1/2005 Muc tieu : —Hoan thien quy trinh trich ly hoat chdt limonoid quy mo pilot, tit cay neem lam nguyen lieu de pha the thu6c bao ve thgc vat, RI dung ba thai sau trich limonoid lam phan bon diet tuye'n va kie'n moi ddt —Dieu the chdt phu gia nhu' chdt lam ban, chdt phu trd de ph6i the vdi limonoid tao thu6c bao ve thtfc vat co hoat tinh manh va ban mai trutrng Nei dung nghien cdu : at Nghien cdu ly trich cac hoat chdt limonoid hat va ld cay neem a Binh Thuan va Ninh Than, dm dung moi CO tinh chon loc cao trich limonoid de dat du'dc hieu sudt cao nhdt Dinh tinh va dinh lurdng cac limonoid de lam cd sa cho viec xay dog quy trinh trich ly limonoid dang pilot b/ Ta' cac ke't qua thu (WO a phan tren, hoan thien quy trinh ly trich hoat chdt limonoid, quy mo pilot hat va IA cay neem lam nguyen lieu pha the thu6c bao ve thu'c vat clang b6t rgc, nhili dau va dung dich Dinh 'dog tong azadirachtin thu6c limonoid (AZL) va azadirachtin (AZ) mOi loci thu6c c/ Dinh phan cac dtrang lieu N, P, K du' lacing limonoid va hdp chdt S ba thai nham sit dung ba thai lam nguyen lieu ph6i the vao phan hitu cd co tac dung diet tuye'n va kie'n m6i ddt d/ Nghien cdu didu the cac phu gia thich hdp nhu chdt lam ban at au dau nanh, chdt phu trd tit can (IL me dd tang hoat limonoid e/ Ke't hdp vdi Trung tam Nghien cdu va Phat tridn Nang Du'dc, Cong ty CP Thu6c Sat Trung Viet Nam va Trung tam Nghien cdu eng dung COng nghe Sinh hoc nhiet ddi Phan vien Cong nghe sau thu hoach de sd khao tdc dung cila thu6c d6i vdi sau mot pha hai kho lifdng thu'c, nain m6c gay benh cho cay va sau bo pha hai vutin rau vutrn cay an trai a Sin phdm nghien ctYu : TT 10 11 Ten sin phdm va chi ti'eu chdt hiring chi yeu Quy trinh cong nghe ly trich limonoid di' la neem Ham liking tang AZL la Quy trinh cong nghe ly trich limonoid ta' dau neem Ham lacing tang AZL cidu Quy trinh Ong nghe ly trich limonoid ttt hat Neem Ham lacing tang AZL hat Quy trinh cong nghe chd bieen ba thai trich limonoid lam phan bon Ham luring N, P, K, limonoid va SOX Quy trinh ding nghe clidu chd Sesam (Sesamin + sesamolin) can du me tinh luyen Ham Wong tang sesamin va sesamolin can dau me Quy trinh ding nghe epoxid h6a dau dau nanh dd didu chd chdt lam bdn vernolat Diking hiring epoxid Ma dau dau nanh San phim Limo 3BR ky thuat - Ham hiring tang AZL hip chit Limo San ph-gm Limo 3ND ky thuat - Ham Wong tang AZL hop chit Limo San phSm Limo 3DD ky thuat - Ham Wong tang AZL hop chdt Limo Chdt tang hoat sesam - Ham hiring tang sesam the ph'Sm Chat b e'n Ma vernolat - Ham hiring vernolat chdpha'm % Mi?c chdt hiking an dqt 0,2 SO' ItAing sin phdm QT % ? 0,2 QT % >_ 0,4 QT % QT % N, P2O5 , K2O ? Limonoid ? 0,01 >1 QT % ? 70 QT % 0,3 kg % 0,3 kg % 0,3 kg % _> 80 0,5 kg % ?_ 80 0,5 kg Ddn vi MIJC LUC Trang T6m tgt Muc luc Danh sach bang va sd a Danh sach hinh PHAN I : Ma DIU PHAN II : TONG QUAN I Gidi thi'eu v6 cay neem I.1 MO to cay 1.2 Ngu6n g6c va yang phan b6 1.3 Gia tri dm cay neem II Tac dOng dol vdi cac loci dich hai II.1 Phtidng thac tac d6ng vao tang 11.2 Doi M.:111g tac clOng III Si( dung cay neem de bao ve thilc vat 5 11 III.1 Hoat chdt limonoid cay neem 11 111.2 M6t s6 che'phSm tit cay neem ten thi treing the gidi 15 III.3 DOc tinh 16 PHAN III : NGHIEN CITU KET QUA VA BAN LU4N NOI DUNG : QUI TRiNH CONG NGHe LY TRiCH LIMONOID Tip LA NEEM HAM LONG TONG AZL, AZ VA S TRONG LA IA Phu'dng pita') va nguyen vat lieu 19 I.1.1 Phudng phdp ly trich va xac dinh ham hicing limonoid 19 1.1.2 Nguyen lieu va hoa chdt 32 1.2 Ket qua 1.2.1 Thanh phan hoa hoc cd ban oh la neem 35 35 1.2.2 Xac dinh kha Wang trich ly limonoid cila mOt s6 dung moi thong dung 36 1.2.3 Qui trinh ly trich limonoid tit la neem Dinh lacing tong AZL va AZ la neem 38 1.2.4 Dinh lacing sulfur 1.3 Ke't luan nOi dung NoI DUNG : QUI TRINH CONG NGH€ LY TRICH LIMONOID 39 40 TRONG DAU NEEM, HAM WONG TONG AZL, AZ VA STRONG DAU 2.1 Phadng phap va nguyen vat lieu 41 2.1.1 Pinking phap tach dau va xac dinh ham lacing limonoid du 41 2.1.2 Nguyen vat lieu, hoa chat 44 44 2.2 Ke't qua 2.2.1 neem 44 2.2.2 Trich limonoid dau neem, dinh Ming AZL, AZ va S dau 2.2.3 Thanh phan acid be() ciia dAu neem 2.3 Ke't luan n6i dung 45 46 47 NOI DUNG : QUI TRINH CONG NG11€ LY TRiCH LIMONOID TRONG HAT NEEM, HAM LUNG TONG AZL VA AZ TRONG HAT 3.1 Phadng phap va nguyen vat lieu 49 3.1.1 Phadng phap ly trich va xac dinh ham lacing limonoid 49 3.1.2 Nguyen lieu va h6a chat 49 50 3.2 Ke't qua 3.2.1 Thanh phan Ma hoc cd ban ciia vo hat neem 50 3.2.2 Thanh phan Ma hoc cd ban cua nhan hat neem 50 3.2.3 Ly trich limonoid banh hat neem, dinh lacing AZL va AZ 51 3.3 K6t Juan n6i dung 53 N6I DUNG : NGHIEN CCU Sit DUNG BA THAI CiJA HAT VA LA NEEM DE PHOI Cid VAO PHAN BON DINH LUVNG CAC THANH PHAN CCi BAN VA DAC TRUNG TRONG BA NEEM 4.1 Phan ba neem 4.1.1 Hi'e'u qua cda viec sit dung ba neem lam phan bon 54 4.1.2 Ch6 bie'n ba neem de lam ph6i lieu cho phan bon 54 54 4.2 Dinh liking cac chi tieu chit lu'Ong 55 4.2.1 Dinh hiking cac chi Weil dac truing ciia ba neem 55 4.2.2 Dinh liking cac chi tieu chit liking ciia ba neem theo 10 TCVN 526-2002 56 4.3 Hieu qua kinh t6 59 4.4 K6t Juan nOi dung 60 NOI DUNG : QUI TRIM! CONG NGIle DIEU CHE SESAM (SESAMIN + SESAMOL + SESAMOLIN) TRONG CAN DAU ME TINH LUYtN 5.1 Dat yin a 61 5.2 Qui trinh cong nghe trich ly hip chit sesam 63 5.2.1 Quy trinh cong nghe 63 5.2.2 Dinh tinh 63 5.2.3 Dinh piing hOn hip sesam 64 5.2.4 Thi6t bi 67 5.3 Hieu qui kinh t6 67 5.4 Thu' nghiem kha nang tang host cila chit phu trd sesam 68 5.4.1 NOi dung thir nghiem 68 5.4.2 Plardng phap did' nghiem 68 5.4.3 K6t qua thii' nghiem 70 5.5 K6t Juan not dung 73 NOI DUNG : QUI TRINH CONG NGI4 EPDXID HOA DAU DAU '' NANH DE DIEU CHE CHAT LAM BEN VERNOLAT 6.1 Dat yin dd 74 6.2 Quy trinh cong nghe didu ch6 chit lam bdn ESO tir d'Au dau nanh 74 6.2.1 Tinh chit va pha'n ddu dau nanh 74 6.2.2 Phan ttng epoxy h6a 75 6.2.3 Qui trinh cong nghe 75 6.2.4 Tinh chit cila au dau nanh epoxid h6a ESO 77 6.2.5 Thi6t bi 78 6.3 Hieu qua kinh t6 78 6.4 Thir nghiem kha nang lam bdn ctia au Vernolat sin pldm Limo 3000 ND 6.4.1 NOi dung thil nghiem 78 78 6.4.2 Pinking phap thif nghiem 79 6.4.3 K6t qua thir nghiem 79 6.5 1(6t luin not dung NOI DUNG : DIEU CHE THUOC BAO Vt THVC VAT Tip LA, DAU 81 VA HAT NEEM 7.1 Dieu ch6 thu6c bao ve thu'c vat tit la neem 7.1.1 Nguyen lieu 7.1.2 Quy trinh ly trich limonoid Id neem de di6u ch6 thu6c bao ye thu'c vat 7.1.3 Di6u ch6 thu6c LIMO 3000BR (0,3%) 82 82 82 84 7.1.4 Gia bin phgm hdp chat limonoid AZL trich la neem 7.1.5 This nghiem tic dung cila thu6c LIMO 3000 BR 7.2 Dieu ch6 thu6c bao ye thu'c vat ttf au neem 7.2.1 Nguyen lieu 85 85 89 89 7.2.2 Qui trinh tach &Au neem bao hoa \fa bat bao hoa a dieu ch6 thu6c bao ve thilc vat 89 7.2.3 Didu ch6 thu6c LIMO 3000ND (0,3%) 92 7.2.4 Gid ban pldm d'au neem Vit bao hoa 92 7.2.5 This nghiem tic dung cua thu6c LIMO 3000 ND 93 7.3 Ditu ch6 thu6c bao ye thqc vat ta' 'Dinh hat neem 7.3.1 Nguyen lieu 95 95 7.3.2 Qui trinh trich limonoid bdnh neem a di6u the thu6c bao ye thiic vat 95 7.3.3 Di6u ch6 thu6c LIMO 3000 DD 95 7.3.4 Gia bin phSm azadirachtin 97 7.3.5 This nghiem tic dung ciia thu6c LIMO 3000 DD 98 7.4 K6t luin nOi dung PHAN IV : KET LIYAN VA DE NGHI TAI LIEU THAM KHAO 101 101 107 PHAN V : PHI) LI,JC Phu luc : Ph6 HPLC cua AZ chugn pha vdi cdc n8ng cl = 1,775 mg/1 c2 = 3,55 mg/1 c3 = 7,10 mg/1 c4 = 14,20 mg/1 110 Phu luc : DO hap thu A = f(c) cua cdc mau la, dau va bdnh neem 118 Phu luc : Ph6 HPLC cua mau la neem 119 Phu luc : Ham lacing S la neem 121 Phu luc : Si bie-n thien cua ham ltfOng azadirachtin va dau beo hat neem sau thu hai va bao quan kho mat 5_7.0 122 Phu luc : Ph6 HPLC cua dd AZ trich tit dau neem 123 Phu luc : Ham lu'Ong S dau neem 125 Thanh phan acid be° dAu neem Phu luc : Ph6 HPLC cua dd AZ bdnh neem 126 127 Phu luc : DO hap thu A = f(c) cua cdc mau ba neem, cao EAE cua la va dau neem 129 Phu luc 10 : Ke-t qua thtr nghiem tdc dung phu trO cua sesam d6i vdi Limo 3000 ND va Vifast 5ND Phu luc 11 : Ket qua tha nghiem tdc dung ben hoa cua vernolat 130 140 Ke-t qua thii nghiem nemg dq azadirachtin tru'dc gia t6c (TGT) va sau gia to-c (SGT) 142 Phu luc 12 : Ket qua thir nghiem thu6c tra' m6i mot LIMO 3000 BR 150 Phu luc 13 : Ke't qua this nghiem thu6c tra' na-rn benh LIMO 3000 ND 156 Phu luc 14 : K.& qua this nghiem thu6c trii sau LIMO 3000 DD 159 DANH SACH BANG SO TEN BANG SO LIEU TRANG II.1 - Tac dung cila hoat chdt limonoid cay neem (16i vCi m6t s6 sau bo - M6t s6 hoat chit limonoid da dttdc trich tit cac b6 phan khac cua cay neem - M6t s6 the phdm didu the tit cay neem dang luu hanh tren thi trtteing the gi5i - D6c tinh cua the Olin Margosan-O dam clac - Thu6c this biet tinh dd xac dinh phAn Ma hoc cd ban - Ke't qua xac dinh phan Ma hoc cd ban cua 16 neem - Ham luring AZL cao trich ly cua 100g b6t IA neem kilo vdi mot s6 dung moi thong dung - Ham lacing azadirachtin trich ly du'dc tit hat neem vdi cac dung moi thong dung khac - Cac chi s6 cua du neem - Thanh pith acid brio tong dlu neem - Ke't qua xac dinh phan Ma hoc cd ban cua vo hat neem - lat qua xac dinh phan Ma hoc cd ban oh nhan hat neem - Ke't qua phan tich cac chi tieu chdt luring cua ba neem dung lam phan bon - Xac dinh su' hien dien cua sesam qua cac phan eng mau - S6 sau song sau xii' ly thu6c Limo 3000ND CT1 va CT2 - S6 sau song tru'dc va sau xit ly 24 giet, 48 gier - Hieu laic thu6c sau 24 va 48 gier - Thanh phan acid beo cua dAu dau nanh - Mat dO du trung loai Tribolium castaneum cac cong thdc till nghiem - Hieu lttc cua thu6c Limo 3000 BR d6i vdi loai Tribolium 11.2 11.3 1.4 2.1 2.2 3.1 3.2 4.1 5.1 5.2 5.3 5.4 6.1 7.1 7.2 a 12 15 17 19 35 36 37 45 47 50 51 59 64 70 72 72 75 86 87 castaneum 7.3 7.4 7.5 7.6 7.7 - Mat d0 loai mot Sitophilus oryzae a cac cong till:1'c till nghiem Hieu IAN cua cac loai thu6c d6i vdi du tang loai Sitophilus oryzae - Ket qua did' nghiem Limo 3000 ND tren na'm Sclerotium rolfsii sau S6 salt song tru'dc va sau )(it ly 24 gid va 48 gier Hieu lu'c thu6c teen sau td sau 24 gier va 48 giei 88 88 93 99 99 - 44S- VIPESCO Irung Tam Nghien cite & Phat trien Nongact dc ph4n Dam Bao Chdt Lu5ng & Thtl Nghiem BAO CAO THCI NGHI:EM Sample ID Filename Method Name Run Time : Azadirachtin 125-1 (Miu LIMO 3000ND c6 Vermolate 1% - Sau GT) - Ham 1trdng : 2366 ppm : CACLASS-VRAzadirachtin 125-1 : C:\CLASS-VP\Methods\AZADIRACHTIN.met : 9/21/2006 2:18:50 PM Data Graph 100 0.075 - u) 0.050 - 025 - 0.000 10 20 40 50 Minutes Run Report Detector A (215nm) I'k # Name 10 II 12 13 14 15 16 17 18 19 20 azadirachtin A 21 azadirachtin B 22 r Totals I Retention Time 2.713 3.733 4.690 4.973 5.387 5.837 6.043 6.710 7.980 8.733 10.280 10.667 11.110 11.537 12.323 13.140 14.347 15.463 17.177 19.163 20.780 22.533 Area 21932169 1752254 18269 11466 32422 2346 9282 179959 134620 42467 23914 12598 8468 27755 143199 63010 26312 101618 68738 1118529 647248 115013 I I Pao, of Height 914206 141262 1808 1184 2075 378 789 6407 6255 1287 1305 760 775 1626 6765 2139 1027 2251 2450 22726 13724 1941 Height Percent 80.68 12.47 0.16 0.10 0.18 0.03 0.07 0.57 0.55 0.11 0.12 0.07 0.07 0.14 0.60 0.19 0.09 0.20 0.22 2.01 1.21 0.17 /150- BAO CAO KET QUA THU.' NGHIEM THUOC TRU MQT NGU C6C LIMO 3000BR Muc dich: Danh gia hien luc diet trir mot cua LIMO 3000BR (0,30%) citii vai mot ngfi c6c pha hai kho luang thuc thuc pham (Sitophilus oryzae, Tribolium castaneum) Anh huang cua thuc den kho lucmg thuc Dieu kien thi nghiern: D6i tucmg : Kho gao Thai gian thuc hien : thang nam 2006 Hoan : thang 09 nam 2006 Dom vi thi nghim : Trung Tam Nghien Cdu va T.Jng Dung C6ng Ngh' Sinh Hoc Nhi"e't Ddi Phirong phap : - 136 tri thi nghi'01: b6 tri mot o gao dien tich 2,5m2 , cho mot luang mot nuoi vao kho, mat d6 thay d6i - Nghi'e'm thirc: c6 nghi@ri thdc g6m: STT Nghie;rn thirc Li'L Luang D6i chdng Khong xir 1Y Limo 3000BR 0,3g/m2 Ric thuc lAn cach Limo 3000BR 0,4g/m2 Limo 3000BR 0,5g/m2 Ric thuc lAn each , : Rac thuc lan cach Phostoxin Cach dung Ric thuc l'An cach Phu luc 12 : Ke't qua this nghiem thu6c trif mai mot Limo 3000 BR _ 4.54— Phtnrng phap quan sat: Tren moi thi nghiem chon diem ngglu nhien theo dung cheo goo, diem theo dOi c6 dinh, each mep 6, t6i thieu 0,5m, khung dieu tra có kich thuac 0,4m x 0,5m Chi tieu theo doi: - Mat d6 mot cho vao truac rac thu6c va 1,4,7,10,13,16,19,21 sau rac thu6c - Tinh toan hieu luc cua thu6c theo cong thirc Henderson Tilton Ke't qua : a) Hi0 Ityc ctia thaw doi voi loai mot Tribolium castaneum - Aftjt cua mgt ode cong there thi nghiem S6 lieu a bang cho thAy, Au tthng mot gao ducc phan b6 kha deu a cac cong thirc vOi mat d6 cao Dieu rat thuan lgi de cac loai thu6c the hien dugc hieu luc C3 giai down dAu, c6 su di chuyen cita Au tir diem qua diem khac, nhien khong &mg ke Ngoai ra, qua qua trinh song, mgt s6 Au tang bi chet to nhien, the hien ro a cong thirc d6i chung Tir 19 sau rai thu6c tra di, hAu het Au da vao nhOng Tinh den thin 21, mat d6 a cong thirc d6i cluang bi giam khoang 23% chet to nhien Cac cong thirc rai thu6c LIMO 3000BR s6 luting Au trUng bi giam tir 78 — 85% Rieng cong thirc (rai thu6c phostoxin, lieu luting 0,4g/m2), s6 lugng Au trimg bi giam 75% so voi tnrac (bang 1) Bang 1: Mat d'6 Au thing loci Tribolium castaneum a cac cong thdc thi nghiem Mat c16 (con/m2) Cong thirc Tnr6c NSR NSR NSR rai 10 13 NSR NSR 16NSR 19 21 NSR NSR thu6c (D6i 77 75 75 73 70 68 66 59 53 chimg) 78 70 60 50 46 40 21 18 12 84 65 55 48 41 36 16 13 76 57 50 42 38 32 12 5 80 68 64 60 53 48 28 23 20 Ghi chi: CTI: &i thing CT2:Limo 3000BR 0,3g/m2 CT3: Limo3000BR 0,5g/m2 CT3: Limo3000BR 0,4g/m2 CT5 phostoxin NSR: Ngay sau rai thu6c - HiO lire oh thuOc: Thu6c LiMO 3000BR a cac lieu lucrng rai tir 0,3g/m 2, 0,4g/m 2, 0,5g/m gay anh htrang manh den mot T castaneum Hieu luc cua thu6c tang cian theo thai gian qua cac Ian xir 1Y S6 lieu a bang cho thAy, sau rai ngay, hieu Itrc chi dat khoang 8% d6i voi lieu rai 0,3g/m2 va 21-23% d6i voi lieu rai 0,4g/m 2, 0,5g/m 2, hieu luc cao hon han so voi thu6c Phostoxin Den thir sau rai, hieu luc len den 21% do'i yen lieu rai 0,3g/m2 (man throng vori thu6c Phostoxin) va xAp xi 40% hai lueu rai 0,4g/m va 0,5g/m Sau raj Ian thin 2, ex thin 16, hieu lkrc cua thu6c LIMO deu dat > 60% ca lieu rai va cao han han so voi hieu lkrc cua thu6c Phostoxin Den 21 sau rai, hieu luc cua thu6c LIMO dat tir 78 — 90% Trong do, hieue luc dia thu6c Phostoxin chi dat den 64% Nhin chung, hieu luc diet Au thing T castaneum cita thudc LIMO tang dan tang lieu rai tir 0,3g/m len 0,4g/m va 0,5g/m Tuy nhien, kitting co su sai khac nhi'eu giira lieu rai 0,4g/m va 0,5g/m Nhtr vay: -453- - Thu6c LIMO 3000BR yeti lieu sir dung 0,3g/m 2, 0,4g/m 2, 0,5g/m tac dOng tot den loci mot T castaneum Hieu luc có xu htrang cao ham so vai thu6c Phostoxin - Hieu ltrc cua thuoc LIMO d6i yen au T castaneum co xu htn:mg tang dan tang lieu rai tir 0,3g/m len 0,4g/m va 0,5g/m Tuy nhien, khong co su sai khac nhieu ve hieu luc gifra lieu 0,4g/m va 0,5g/m Bang 2: Hieu luc cua thu6c LIMO 3000BR diSi vai loaI Tribolium castaneum Heu luc dm thu6c (%) Cong NSR NSR 13 NSR NSR NSR 21 NSR 21,34 b 32,32 a 35,08 b 42,46 be 68,74 b 69,84 c 77,74 b 21,06 a 33,42 a 39,50 a 46,26 a 51,84ab 77,90a 79,80 b 86,36a 23,20 a 33,22 a 40,54a 45,22 a 53,00a 69,70 b 86,84a 90,38a 12,94 b 17,56 b 20,80 b 25,24 b 38,00 c 59,46 c 62,42 d 63,76 c CV(%) 18,64 22,89 21,09 11,06 15,86 15,86 6,14 4,83 LSD () 05 4,09 8,10 9,41 5,63 9,85 6,05 6,15 5,15 thirc NSR 8,24 Ghi NSR c CTI: dai chfrng CT2:Limo 3000BR 0,3g/m2 CT3: Limo3000BR 0,5g/m2 CT3: Limo3000BR 0,4g/m2 CT5: Phostoxin NSR: Ngay sau raj thu6c b) Doi veri loai Sitophilus oryzae - Mat mot er clic cong Mix thi nghiem Ttrcmg to nhtr loci T , mat d6 cita loci mot S cling có mat d6 cao va phan b6 deu a clic o va clic cong thirc thi nghiem S6 Itrang mot cling bi giam clan qua trinh thi nghiem vai mot tY le chet to nhien khoang 7% sau 10 va 18% sau 21 Ty le chet to nhien kha cao tac etc:mg ca hoc qua trinh lam thi nghiem _ 154- Bang 3: Ivgt d6 loci mot Sitophilus oryzae Cong cac cong thirc thi nghirri M'a't d9 (con/m2) thirc Tnrac NSR NSR NSR rai 10 13 NSR NSR 16NSR 19 21 NSR NSR thu6c (D6i 101 98 97 97 93 92 88 83 83 chimg) 113 92 83 75 69 58 37 29 20 110 84 78 68 60 50 30 25 17 116 80 67 59 51 46 24 21 14 112 97 88 86 76 66 42 38 38 Ghi chz: CTI: dl i chzing CT2:Limo 3000BR 0,3g/m2 CT3: Limo3000BR 0,5g/m2 CT3: Limo3000BR 0,4g/m2 CT5 Phostoxin LEO hrc cira thutic Bang 4: H*11.rc cua cac loci thu6c d6i voi au trimg loci Sitophilus oryzae Cong Hieu hrc ctia thu6c qua cac thoi lc , diL tra thirc NSR NSR NSR 10 NSR 13 NSR NSR 16,58 b 23,98 b 31,48 b 34,40 b 44,10 b 59,68 bc 66,56 bc 78,28 b 21,18a 31,92a 41,18 a 45,24a 53,96a 69,24ab 74,20ab 81,04ab 29,40a 36,50a 44,16 a 49,24a 53,50a 75,30a 76,02a 84,98a 10,84 c 17,62 c 19,80 c 26,12 CV(%) 19,15 16,46 11,72 12,90 11,51 LSD 0.05 5,01 6,07 5,37 6,70 7,20 Ghi chic: CTI: dai chirng c 35,18 c 57,10 c CT2:Limo 3000BR 0,3g/m2 CT3: Limo3000BR 0,5g/m2 NSR 21 NSR 58.54 c 58,62 c 11,32 9,20 5,61 9,16 8,49 5,69 CT3: Limo3000BR 0,4g/m2 CT5: Phostoxin NSR: Ngay sau rai thu6c 455— Thu& Limo 3000BR a cac lieu luting 0,3g/m 2, 0,4g/m 2, 0,5g/m the hien hieu luc kh a cao d 6i vai loci inOt S oryzae Sau phun ngay, hieu luc cua thu6c dat khoang 17% di yeti lieu rai 0,3g/m va 21 — 29% di vai lieu 0,4g/m va 0,5g/m Hien luc cUa ca lieu rai deu cao hon Ilan so vai hieu Luc cua thu& phostoxin trogn cimg thdri gian Den thin sau rai, hieu luc cita thu6c LIMO dr tat ca cac lieu deu dat > 30% (31, 41 va 44%) Trong hien ltrc cua thu6c Phostoxin chi dat den 19,8% Hieu luc cim thu& tang clan va dat 78% - 85% a thir 21 sau rai, cao han han so vai thu6c Phostoxin, chi dat 58,62% S6 lieu a bang cling cho thay, hien luc cua thu6c LIMO di vai au loci S oryzae c6 xu huong tang dan tang lieu rai tir 0,3g/m len 0,4g/m 2va 0,5g/m Tuy nhien, kh8ng c6 su sai khac ro gift lieu 0,4g/m 2va 0,5g/m 2, 2, Nhu vay,i thu6c Limo 3000BR sir dung a lieu rai 0,3g/m 0,4g/m 0,5g/m c o hi eu I uc t 6t d 6i v dri Au mot Sitophilus oryzae Hieu hrc cao han han so vai thu6c Phostoxin Co the sir dung LIMO 3000BR lieu rai 0,4g/m a tit Au tang mot Sitophilus oryzae Ket luAn chung: Thu& LIMO 3000BR co hieu luc tot d6i vai Au thing ca hai loci Tribolium castaneum va Sitophilus oryzae Hieu Igo tot han so vai thu& Phostoxin Thu6c LIMO 3000BR dr lieu rai 0,4g/m co the sir dung de trir Au trimg mot Tribolium castaneum va Sitophilus oryzae c) Anh huirng ctia thac den kho thtyc: Qua phan tich xac dinh cac limonoid IchOng cua kho thi nghiem cac 7,14 va 21 bang phucmg phap sac ky khong phat hien co su hien dien cac chat kho Tp H6 Chi Minh, 25 thing nam 2006 Ngtrai thuc hien Nguygn Ngir 436 - CTY.CP THUOC SAT TRONG VIt‘ T NAM TRUNG TAM NC &PT NONG DUC/C *** BAO CAO KET QUA THI! NGHIEM SINH HOC HIEU LIJC CUA LIMO 3000 ND TRif NAM HAT CAI SCLEROTIUM ROLFSII WC DtCH: Khdo sat n6ng d5 hffu hieu ctia skin phftm Limo 3000 ND nham t?c chg sink tru'ang ctia na-m hat cai sclerotium rolfsii gay benh lc di re" cho cay NCI DUNG- PHUVING PHAP 2.11-loa chgt : Haa chgt Limo 3000 ND (ngay 28-8-2006 ) NCing d() thd nrghiem ( % ) 0.1 0.2 0.3 0.4 0.5 2.2Phtidng phap thti' nghiem : - PhUdng phap Agar dilution : hoa thu6c vac) moi trtaing nuoi na-m PGA dia petri Sau cay hach ngm Slerotium rolfsii va theo dOi ski Oat trign ctia ngm tren moi trtaing thii' Moi nghiem tilde grim lisn nhac, mai tan nhac g6m1 dia petri Chi tie u theo doi : theo dOi ski Oat trign cua khugn ty ngm Tinh T5/ le tic chg khugn ty I = ( — Dtre5ng kinh khugn lac thit nghiem) X 100 Dtteing kinh khugn lac d6i clang Phan tich th6ng ke danh gig mti'c d8 tin cay (Ala thi nghiem bang chu'ong trinh MSTATC Phu luc 13 : Ke-t qua thil'nghiem thu6c trit nain benh Limo 3000 ND -457 KET QUA : Ban : ke't yua th4 nghiem Limo 3000 ND tren nam Sclerotium rolfsii sau Nghirn thee Du'Cing kinh trung binh ctia khugn I4c ( mm) S au Sau Sau Sau ngay ngay LIM03000ND 0.1% 13 28.25 0.2% 5.5 0.3% 0.4% 39.25 %I (sau4ngay ) b 51.24 15.75 28.75 be 64.28 16.75 23.25 be 71.12 5.25 14.25' cd 82.29 0.5% * 5.00 d 93.79 1% 0 0.00 d 100 32.75 57 80.50 a - DoICHEING CV % = 6.5 % LSD 0.01 = 17.52 Ghi chu : ( * ) hach lam vita mdi m'Am • NhCin xet : Theo dOi str phat trien cua khugn ty ngm sclerotium rolfsii ngay, chting tOi nh4n thgy nghiem thac (16.i hach ram nay' ram Om sau 24 giO Nig khugn ty Oat trien nhanh ehong tr'en be mat moi trit(Ing nuoi, %/Ong -45S- cac nghiem thtic co xd ly Limo 3000ND tat 0.1% - % d6u co tac thing (len su' phat Eden ctia khugn ty, Limo 3000ND vdi ruing di5 iy 0.5% va % cho hieu yua tic ch6 khugn ty cao nhcit ( 93.79% - 100% ), khac biet hlin so vdi cac niIngc10 khac KET LU4N- DE NGHT: Ket Juan : khdo sat tic clOng ctia Limo 3000ND d6i vdi na-m Sclerotium rolfsii Chung toi nhan tha-y : Limo 3000 ND vdi n6ng d6 thit nghiem tit 0.1% de-n.1% den co tac dung kie'm ham su' Oat trien cda khugn ty na-m, CIO nOng dO xi? 1.5", Limo - 1% co tac dung tic 66100 % su'n‘y ma'm cua h4ch na -m va sir phat trien cda khan ty nghi : CO the al dung Limo 3000ND vdi n6ng dO la % de phong tnY natm Sclerotium rolfsii gay BUT re tren cay Tuy nhien, trade ling dung san phfm vao thtic t6 chting to can phai ti6p tuc the nghiem ngoai nha htdi va dOng ruotig de dank gia lai hi6u lu'c thu6c va hieu cida kinh t6 stt dung Limo 3000ND, thing thoN khao sat them AIM ctia thu6c d6i vdi cay PHZ) TRUNG TAM Cl IAM TP.HCM, 14 thang nam 2006 Ngttdi thtic hien r NGNIEN CIJU VA vi RAT MIEN KONG otkic , ti )1(1/C/01' * cfms recd P.HAV• 4?I THUO2 SAT TRUNG /0 VIgT NAM `-! 1):_'TP HO d 72) (.1 4'xit (7."-P PHAM MINH TAM PHAM VAN THANH -459— COng Ty Phan Thu& Sat Trung Viet Nam TRUNG TAM NC & PT NONG DUCC Bao Cao Ker Qua KHAO ThtY Nghiem SAT HleU LAIC THUOC TRU SAU LIMO 3000ND Trong PhOng Till Sau To' (Plutella Xylostella)H0iy Ho Th1p TV Trong PhOng Thr NghiOrn I Muc Bich: Danh gia hieu tic diet sau dia Limo 3000ND (0.30%) d6i %/di sau to (Plutella Xylostella) hai cay ho thgp ti/ phOng thi nghiem II Dieu kien kith) nghiem: Thdi gian thuc hien : tit 11 den 18 thang nam 2006 Dia dim khao nghiem : PhOng nuOi sau to thu6c Trung Tam NC & PT Wing DLIOC Don vi ph6i hop : Trung Tam NC & PT NOng Difoc — Cong Ty C6 Phan Thu6c Sat Trung Viet Nam III Phtidno Phil) Kt& Nghiem: 86 tri : Thi nghiem difdc ti6n hanh bang cach nhung 'rot dgu la cii (cii ngot ) 7cm dung dich thu6c, sau dO dat vao dia petri co lOt giay gm, bat tha vgo m6i (Ira 10 sau to sting khoe manh tu6i S6 Ian nhic cua m6i Ong thitc : Ian S6 Ian lap lai : Nghiem thk : CO nghiem thin gam : Nghiem thfic Limo 3000ND (Mill 1) Limo 3000ND (Mb 2) Vineem 1500ND Limo 3000ND (M3u 1) Limo 3000ND (Miu 2) Vineem 1500ND D 6i chfing Ong di) (%) 1 2 Ntroc la ` Ghi chi': - Limo 3000ND (Miu 1- CO Synergist - 05/09/06) - Limo 3000ND (Mitt — KhOng co Synergist - 05/09/06) - Vineem 1500ND ( C6ng Ty C6 Phan Thu6c Sat In-mg viqt Nam — 280505E1) IV Chi tido theo doi : De'm s6 sau song m6i dia petri sau xii Ict thu6c 24 gid va 48 gid Hieu Ikrc thu6c dtroc tinh theo cong thilc ABBOTT E = Ca — Ta x100 Ca E : Hieu IVc cua thu6c tinh bang % Ca : Muc gay hal o 16 d6i chirng sau Ulf!' nghiem thu6c Ta : Mirc gay hai b 16 01 nghiem sau thu nghiem thutic Phan tich s6 lieu theo phttong phap th6ng kg MSTATC Phu luc 14 : K6t qua this nghi@n thu6c trii sau Limo 3000 DD _ 460_ V KO Qua - Nhan xet : Bang : So- sau song trddc A sau xii Ili 24 gid A 48 gid Nghiem thin Limo 3000ND (Mau 1) Limo 3000ND (Mau 2) Vineem 1500ND Limo 3000ND (Mau 1) Limo 3000ND (Mau 2) Vineem 1500ND D6i ch(lng N6ng d6 (%) 1 2 Not la S6 sau sting /3 ea petri Tn.& xi't 19 Sau xill9 24h Sau xill9 48h 30 21 17 30 25 22 30 24 21 30 14 10 30 19 15 30 18 14 30 30 30 Bang : Hieu Idc thueic tren sau to sau 24 gid A 48 gib Nghiem thitc Limo 3000ND (Mau 1) Limo 3000ND (Mau 2) Vineem 1500ND Limo 3000ND (Mau 1) Limo 3000ND (Mau 2) Vineem 1500ND Cie/0 0.01) Trong cling mot cot, nhrtng Least Significant Difference Test Wong d6 (%) 1 2 Hieu k thu6c (%) Sau xi1l9 24 gid Sau xi1 ly 48 gid • 30.00 c 43.33 c 16.67 b 26.67 b 20.00 b 30.00 b 53.33 e 66.67 d 36.67 cd 50.00 c 40.00 d 53.33 c 5.54 7.25 0.9945 1.110 s6 co ding chit khOng khac biet CO jr nghia mtic d6 1% theo Nhan xet Theo Idt qua th6ng ke tren MsTatc, sau 24 gid va 48 gib thif nghiem, chOng tei co nhiing nhan xet sau : - CO so khac biet co nghia gifa nghiem thk Limo 3000ND (mit] 1) vdi Limo 3000ND (mail 2)(nOng d6 1% va 2%) va Vineem 1500ND (n6ng d6 2% va 4%) - KhOng co su khac biet co nghia giita nghiem thOc Limo 3000ND (rniu 2) vdi Vineem 1500ND cac nong d6 thin nghiem VI KA Juan : - Hieu litc diet sau to hai cay ho thap to cua Limo 3000ND (mail 1) cao hal so vdi Limo 3000ND (mai' 2) (ming d6 1% va 2%) va Vineem 1500ND (n6ng d6 2% va 4%) - Hieu tic diet sau dm limo 3000ND (mil] 2) (n6ng d6 1% va 2%) toong duong voi Vineem 1500ND (n6ng d6 2% va 4%) Tp.H6 Chi Minh, 18 thang09 nam 2006 PtiO 1) 30 thud hien 1:)• ".'• TRUNG TA NGHIENCliU PHA T Tfileixam * v (1./- coNG \ • HA TH DO Pham Thanh Trang C SAT TRUN VIET , 11/ 13 rP Heic.:` ; - - v C , :,i, 't' ,k` ./if,,,efr? • _464_ Data file: LIMO 3000ND Title : SAU 48 GIO Function: ANOVA-2 Data case to 21 Two-way Analysis of Variance over Variable (NGHIEM THUC) with values from to and over Variable (LAN LAP LAI) with values from to Variable 3: SO SAU SONG SAU 48 GIO ANALYSIS Source O F VARIANCE Degrees of Sum of Freedom Squares NGHIEM THUC LAN LAP LAI Error Non-additivity Residual 12 11 85.90 0.29 2.38 0.32 2.06 Total 20 88.57 Grand Mean = Grand Sum = Total Count = Coefficient of Variation= Mean Square 14.317 0.143 0.198 0.321 0.187 6.143 129.000 21 7.2535 Means for variable (SO SAU SONG SAU 48 GIO) for each level of variable (NGHIEM THUC): Var Value 1• Var Mean 5.667 7.333 7.000 3.333 5.000 4.667 10.000 Means for variable (SO SAU SONG SAU 48 GIO) for each level of variable (LAN LAP LAI): Var Value Var Mean 6.000 6.143 6.286 lsd at 0.05 alpha level = S 0.519 TABLE F-value Prob 72.16 0.72 0.0000 0.5066 1.71 0.2174 _ Data File : LIMO 3000ND (Sau 48 gio) Function : RANGE Error Mean Square = 0.1980 Error Degrees of Freedom = 12 No of observations to calculate a mean = Least Significant Difference Test LSD value = 1.110 at alpha = 0.010 Original Order &S is it is Mean = 5.667 Mean 2= 7.333 Mean = 7.000 Mean = 3.333 Mean = 5.000 Mean = 4.667 Mean = 10.00 Ranked Order C Mean = 10.00 B Mean 2= 7:333 B B Mean = 7.000 B Mean = 5.667 C C Mean = 5.000 C C Mean = 4.667 C Mean = 3.333 D A A D .Oq upon run !tau `uvq LTA 2uvq tuaq2u 2uo4d EI:13 C 2uop ris aonp 2uotpi nail claw ors atop 2uo4}i -Suet{ of 043 in Erg oo guotri new !rm !op in p!2 oo !43 tuarq2u nip ?nb NOS GOON 109 2upga.1 - Dyfo doloail0 u*142u Pill DP uz?!O 900Z.60.0Z XV2u INDF1 di / coE Z / 669 Z wdd 'uptprillpezV 1H 1DS /101 - 31N/101/013A DINIOHN 99£ Z / 8/.£ Z wdd'u!Itlaei!Pt7zV 1H 1DS /101- 31V1OINS3A %I / sl-InPoxl-018 ens ixid OPPI WEILL 90L z /586 Z wdd `wlIpri!PriV 1H IDS /101 - 110 INVS3S %Z / aouaiafty / poripui owl ynsay vidap4,) allI1711 ip,717E( asp u3lAgur) drid au.onqd fnb Jam nai9 pi3 nal Suvq of au mui-kind aT3 uo!spqa 011000£ 00/11 Sups?, fo linsay 114!Ou n41 ?nb la}{ '9 Swam Jo ma 900r60.0Z 79 61 '91 'SI 111 11 2u 1141 AV2N 900Z.60.L0 new uequ Av2N idraoad fo amp maga fo ssa4ppv pun autoN nr13 11?IlrilIsi tun 2un11 2url E3TP111.13 rip VA 11?1 £ uoydLosap aidungs '9 13d no Swan un43 `flu 2uwi new rtil oc Sued new E1 9IAI rum nal fo atunN n41 nEU1 11?1 • DN.LL- v ZL / (L.Z1 ZrI) CIN000£ 01A11-1 90 / S3c11A / OLV I oN J I/ 9S /2tre11 90 /1.I.D21'dO gal x!PuaddV : ( isocm 11131H9N 5111.E yab 11I1-1c1 ago," nOess .antewswecetze.aeacestmobtsrecutwaxwoma-6.1a, Isr.i,99436* : N3H '13P1:011 ) 1617Sc;cj6 11) IeoilS 101 al LZL triwnflauset ;$.:c.)Ct,*(:P 191 - 0006 OSI ANtitiNOD Lf,l(3)1 I.Elf;, 31113I1S3d VN131A NVHd Cr,) e~4M it - F91' - !INN;