... detect Aly ⁄ REF and Rh transcripts were for Aly ⁄ REF: sense, 5¢-CTGGTCGCAGCTTAGG AACAG-3¢ and antisense, 5¢-AATGTTCATGGGGCGGC CATC-3¢, for RH: sense, 5¢-GCAACGATACCCAGTTT GTC-3¢ and antisense, ... we prepared the following mutant probe: biotin- 5¢-GGGACTATGAT GATGGGTTTGTGAGGAAATGT-3¢ and 5¢-ACATTTC CTCACAAACCCATCATAGTCCC-3¢ HEL cell nuclear proteins carried by the probes were separated using ... (Fig 2B) For supershift assays, lL of rat mAb antiGATA1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was added Samples were incubated for 30 at room temperature and resolved on 5% acrylamide...