Báo cáo y học: "Central fatigue and nycthemeral change of serum tryptophan and serotonin in the athletic horse" potx

... follows: tryptophan at18:45 in the first day and at 18:16 in the second day; sero-tonin at 19:00 in the first day and 18:24 in the second day.Daily rhythm of serotonin blood level in the horseFigure ... Poncet L, Denoroy L, Jouvet M: Daily variations in vivo tryp-tophan hydroxylation and in the contents of serotonin and 5-hydroxyindoleacetic acid in discrete brain areas of the rat. JNeural ... blood serum concentrations of some neutralamino acids and serotonin in the horse: a preliminary study. In Proceedings of the symposium "Biological rhythms in livestock" Messina,Italy:95-99....

Báo cáo y học: " Agent-based dynamic knowledge representation of Pseudomonas aeruginosa virulence activation in the stressed gut: Towards characterizing hostpathogen interactions in gut-derived sepsis" pot

... role in determining extent of host injury. As noted above, the currentGMABM highly abstracts the host functions concerning the dynamics of maintaining the mucus layer and, particularly, the immune ... detailed instantiations of these mecha nisms as well asalternative hypotheses in the G MABM. The behavior of the ABM may then suggest the type and design of experiments to test and validate these hypotheses, ... thereby enhancing the throughput of hypothesis evaluation. Of course, the direction of the thought experiments would be informed by the researcher’sexpertise and intuition. For instance, in the...

Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt

... IR. Results of each values were expressed as the following ratio: intensity of WD cDNA/intensity of competitor and then divided by intensity of ampliﬁed actin cDNA. PC, the result of competitive ... (underlined basesdenote the functional core of the MREs and the Sp1 bindingelement, and the mutated bases are indicated by italictype).Western blot analysis The MREa-binding proteins puriﬁed ... determine whether the MREa–protein complex displays sequence speciﬁcity. Binding of the HepG2 nuclear proteins to MREa was inhibited by the addition of a molar excess of unlabelled MREa to the binding...

Báo cáo Y học: Mammalian mitochondrial endonuclease G Digestion of R-loops and localization in intermembrane space doc

... concentration of endoG for 10 min at 37 °C. The reaction was stopped by the addition of 1 lgÆmL)1prote-inase K and 0.5% SDS, and then incubated for another10 min. R-loops were analyzed by 0.7% agarose ... region(Fig. 3A). The positions were further localized by primerextension. The cleavage signals were mapped to the guanine-rich region in CSB II of the mitochondrial heavystrand in the ordinary supercoiled ... 10% glycerol, and 6Murea. The denatured His-endoG was renatured on the resinby sequentially reducing the urea in the buffer from 6Mto0M, in 10 steps. The renatured His-endoG was ﬁnally elutedwith...

Báo cáo y học: "Reactive oxygen species induce expression of vascular endothelial growth factor in chondrocytes and human articular cartilage explants" pps

... PMA or SIN-1 resulted in a dose-dependent upregulation of the VEGF protein (as determinedusing ELISA) and an increase in the level of NO in the medium.Our findings indicate ROS-mediated induction ... especially the surroundingsynovial tissue. This reflects the findings of Felson and coworkers [27], who declared OA to be a disease involving the whole joint. In summary, these data support the role ... SIN-1 (using ELISA)To determine whether the increased levels of VEGF mRNAwere reflected in the production of protein by chondrocytes,an ELISA was performed to quantify the VEGF content in the medium...

Báo cáo y học: " MM-ChIP enables integrative analysis of cross-platform and between-laboratory ChIP-chip or ChIP-seq data" doc

... enrichment of the ER bi ndingmotif to evaluate the quality of the inferred enrichedregions. By mapping the occurrence of the ER bindingmotif within a 500-bp window surrounding the identi-fied ... canonicalCTCF binding motif is plotted as a function of the number of top-ranked binding sites for both the individual and combined datasets. The results of integrative analysis using the region intersection ... enrichm ent of the canonical bindingmotif of CTCF to evaluate the perfor mance of MM-ChIP for ChIP-seq peak detection. By mapping the occurrence of the CTCF binding motif within 50 bp of the identified...

Báo cáo y học: "Comparative whole genome sequence analysis of wild-type and cidofovir-resistant monkeypoxvirus" doc

... domain lies at the juncture of the catalytic domain and the outside medium and is thought to mediate NTP entry and selection and support exonuclease proofreading [40]. The funneldomain may mediate ... ismediated by the C-terminal active domain of D1R.Triphosphatase and quanylyltransferase activity arelocated within the N-terminal domain [53]. Followingheterodimerization, the stimulatory D12 subunit ... Insights into ATP selectivity and processivity. Mol Cell 2006, 33:339-349.64. Deng L, Gershon PD: Interplay of two uridylate-specific RNA binding sites in the translocation of poly(A) polymerase...

Báo cáo y học: "B-lymphocyte stimulator/a proliferation-inducing ligand heterotrimers are elevated in the sera of patients with autoimmune disease and are neutralized by atacicept and B-cell maturation antigen-immunoglobulin" pdf

... role in the inflamed synovialcompartment [15,16,38]. It would be of interest torepeat these studies of matched RA serum and synovialfluid samples by using our APRIL assay.Our analysis of the ... similar binding proper-ties and in vitro activities to the BLyS and APRIL homo-trimers, and may be present in sera in similar amounts toAPRIL and BLyS, we postulate that they may likely playsimilar ... of assay buffer. Wells werethen resuspended in 100 μl of assay buffer and analyzedby using a Luminex 100 mac hine (Luminex Corporation,Austin, TX, USA). The total assay time was 2 hours. The assayhadabroadrange(~100pg/mlto25ng/ml)withan...

Báo cáo y học: " Association between reduced bronchodilatory effect of deep inspiration and loss of alveolar attachments" pps

... carried the clinical and functionalassessments and participated to the interpretation of the data; AMV participated to the design and the coordination of the study and the interpretation of the ... con-ceived and participated to the design of the study, the analysis of the data and the interpretation of the results, and contributed significantly to draft the manuscript; VBhelped in the design and ... is also in agreement with the report byPathology picture showing the intact (a) and the destroyed (b) alveolar attachmentsFigure 4Pathology picture showing the intact (a) and the destroyed (b)...

Báo cáo y học: "Parenteral versus enteral nutrition: effect on serum cytokines and the hepatic expression of mRNA of suppressor of cytokine signaling proteins, insulin-like growth factor-1 and the growth hormone receptor in rodent sepsis" pps

... signaling-2 and suppressors of cytokine signaling-3PCR measurements for cytokine-inducible SH2-containing protein, sup-pressors of cytokine signaling-2 and suppressors of cytokine signaling-3. ... nutrition and the route of nutrition in sepsis dif-ferentially influence circulating cytokine profiles and the expression of mRNA of SOCS proteins, of the GHR and of IGF-I. The present study demonstrates ... the level of the GHR or be associated with a change in the intracellular sign-aling pathway for GH. The induction of SOCS proteins by hormones and/ orcytokines has been hypothesized to inhibit...

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