Báo cáo khoa học: "Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing" pdf

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Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt

... or without hematoxylin staining. In hypoxia experiments, 3T3-L1 cells were maintained in a hypoxia chamber (Invivo 400; Ruskinn Inc., Cincinnati,OH, USA) constantly maintained at 1% O2. Culturemedium ... cultured in DMEM containing 10% fetal bovineserum. OP9 cells were grown in aMEM containing 20%fetal bovine serum. C2C12 cells were maintained in DMEMcontaining 10% fetal bovine serum.Adipogenic ... serumand IDM (10 lgÆmL)1insulin, 1 lm dexamethasone, and0.5 mm IDM). Cells were then maintained in DMEM con-taining 10% fetal bovine serum and 1 lgÆmL)1insulin. Themedium was replaced...

Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

... interact with a number of functionalgroups, including the aromatic side chains of someamino acids, through a stacking mechanism [42]. Theinteraction of arginine with aromatic amino acids of aB-crystallin ... that aB-crystallin most likelyemploys various methods of binding (or bindingmodes) in order to prevent the aggregation of stressedproteins. Some of these binding modes (or bindingsites) are ... theactivity of aB-crystallin against DTT-induced precipi-tation of insulin at intermediate and high concentra-tions, and it also increased the activity of aB-crystallin in preventing the aggregation...

Tài liệu Báo cáo khoa học: Catalytic mechanism of SGAP, a double-zinc aminopeptidase from Streptomyces griseus pdf

... N-terminal amine is in con-tact with one of the zinc ions [26,27]. This mode of binding in SGAP still allows the oxygen atoms of thegem-diolate intermediate to be stabilized by interactingwith ... amine group of the leaving peptide bringing to the break-down of the intermediate. Dashed lines indi-cate stabilizing interactions and ⁄ or hydrogenbonds in the catalytic pathway; Pep, theincoming ... differ in severalaspects, including the exact role of the two active sitezinc ions in catalysis, the detailed sequence of zinc-coordination changes during catalysis and the mode of inhibition of...

Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

... detected at the dimerinterface of the models or in the surroundings of themutated residues. The models of RNase A covalent dimers in the exchanging conformation were prepared using the7RSA structure ... detected atthe dimer interface of the models or in the surroundings of the hinge loop residues. Likewise, the models of nonex-changing and exchanging HHP-RNase were prepared start-ing from 1Z7X. ... prepared using the 7RSA structure and the struc-ture of nonexchanging BS-RNase (1R3M) as template.Brieﬂy, two molecules of RNase A were superimposed tothe subunits of BS-RNase using the ﬁt tools of...

Tài liệu Báo cáo khoa học: Structural characterization of Ca2+/CaM in complex with the phosphorylase kinase PhK5 peptide pdf

... extension to the protein kinase folds backon the kinase domain and interferes with the substratebinding sites. In the case of the titin and twitchinkinases, the autoinhibitory sequence acts ... hexadecameric enzyme consisting of four copies of four subunits: (abcd)4. An intrinsic calmodulin (CaM, the dsubunit) binds directly to the c protein kinase chain. The interaction site of CaM on c has ... PhKcsubunit encodes a C-terminal extension to the proteinkinase domain and treatment of the kinase withKeywordscalmodulin; kinase regulation; protein–protein interaction; NMR spectroscopyCorrespondenceJ....

Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafﬁcking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efﬁcient GABA-uptake activity of the GABA transporter pptx

... substratebinding process. This provides strong evidence thatN-glycans, in particular their terminal structures, areinvolved in regulating the GABA translocation of GAT1, but not in binding of GAT1 ... remaining[3H]GABA and [14C]sucrose. The protein concentration in the supernatant was determined using the bicinchoninicacid protein assay reagent (Pierce, USA). Unspeciﬁc uptakewas determined ... not the terminaltrimming of N-glycans is involved in the regulation of the stability and trafﬁcking of GAT1. It has beenreported that a variety of molecular chaperones andfolding enzymes assist...

Tài liệu Báo cáo khoa học: Improving Classification of Medical Assertions in Clinical Notes" pdf

... the training data to measure the impact of each of the four subsets of features explained in Section 3. Ta-ble 3 shows the cross validation results when cu-mulatively adding each set of features. ... Syntactic Features: Part -of- speech tags of the three words preceding the medical term and the three words following it. 312create one classifier for each type of label using instances with that ... features including negative pre-fixes and binary tf-idf features primarily increased performance on the absent class. Using document frequency to prune lexical features showed small gains in all...

Tài liệu Báo cáo khoa học: "Topological Ordering of Function Words in Hierarchical Phrase-based Translation" pdf

... Y)326NN = 2048N = 128N = 64N = 2048331Proceedings of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 324–332,Suntec, Singapore, 2-7 August 2009.c2009 ACL and AFNLP1...

Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx

... eosin staining. We examined the expres-sion of these proteins in a cancer-free layer of squamousepithelial cells, carcinoma in situ (CIS) and invasive cancer,all of which were observed in ... detected notonly in total cellular proteins but also in chromatin-boundproteins. The Mcm proteins bound to chromatin probablyfunction in DNA replication, but the role of the proteins nottightly ... blotting using anti-Mcm2, 3, 4, 5,6 and 7 Ig (Fig. 1). On comparing the amounts of Mcm in total cellular proteins and chromatin-bound proteins,approximately one-third of total Mcm protein was...

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