... concentrations was followed by measure-ment of the remaining activity towards casein or the synthetic substrate 2-aminobenzoyl-Ala-Gly-Leu-Ala-4-nitrobenzylamide (Abz-AGLA-Nba), as described in the Experimental ... to conformational andautoproteolytical stabilization. Particular attention ispaid to the role of calcium ions. For this reason, anenzyme variant with a mutation in the vicinity of cal-cium ... 1181–1189.8 Veltman OR, Vriend G, Hardy F, Mansfeld J, van denBurg B, Venema G & Eijsink VG (1997) Mutationalanalysis of a surface area that is critical for the thermalstability of thermolysin-like...