Báo cáo khoa học: Protein engineering of pyruvate carboxylase Investigation on the function of acetyl-CoA and the quaternary structure doc
Báo cáo khoa học: Protein engineering of pyruvate carboxylase Investigation on the function of acetyl-CoA and the quaternary structure doc
... respectively, and the elution profile was not dependent on the protein concentra-tion. (C) and (D) The elution profile of PC-(BC) was markedlydependent on the protein concentration: at the high concentration[100 ... ambienttemperature and analyzed.ResultsConstruction and purification of the engineeredproteins of PC The boundary of the BC and CT domains of PC wasestimated as follows: the BC subunit of E. coli acetyl-CoA carboxylase ... carboxylase Investigation on the function of acetyl-CoA and the quaternary structure Shinji Sueda, Md. Nurul Islam and Hiroki KondoDepartment of Biochemical Engineering and Science, Kyushu Institute of Technology,...
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
... hydroxyls and the protein backbone stabilized the interaction. The orientation of this glucosyl residue indicated that itcorresponded to the nonreducing terminus of the oligosaccharide. The other connected ... for the F144A mutation.Examination of the crystal structure of the F258Imutant showed it to be isomorphous with native Exgother than a small adjustment in the backbone confor-mation of loop ... evaluation of the nitrogen assimilation tests commonly used in the classification of yeasts. J Bacteriol 52, 392–401.40 Peterson GL (1977) A simplification of the protein assay method of Lowry...
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Báo cáo khoa học: Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation / hydrolysis ratio pptx
... out) and introduced (SalI, only in GTFA, and SacI) for con-struction of the various hybrid proteins is indicated. (C) Amino acidnumbering of GTFA and GTFO deletion mutants (consisting only of the ... present in the glucan binding domains of GTFA and GTFO [4,5]; black and whitebars, predicted locations of the a-helices and b-strands, respec-tively, corresponding to the relative position of these ... In the present study, we report adetailed analysis of various hybrid GTFA ⁄ O enzymes, resulting in the iden-tification of specific regions in the N-termini of the catalytic domains of these proteins...
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Báo cáo khoa học: Protein aggregation and amyloid fibril formation prediction software from primary sequence: towards controlling the formation of bacterial inclusion bodies pot
... toadopt the same architecture under physiological condi-tions, as part of their functional role ([4–8] and refer-ences therein), despite the diversity of origin of theirconstituent proteins. ... residue of the protein, equal to the probability of backbone–back-bone hydrogen bond formation observed for this type of residue; then, the obtained values are averagedinside the window and the ... regions. On the smoothedprofile, a region is predicted as an amyloidogenic oneif all its residues lie above a given cut-off and the size of the region is greater than or equal to the size of the sliding...
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Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx
... structural analyses and the construction of artificial libraries allow con-structive selection and functionalization not only of antibody fragments, but also of small, nonantibodyproteins (Fig. 1). ... describes the methodology forelucidating protein protein interactions and selectingspecific binders to novel target proteins, and the first and second minireviews focus on the detection of protein protein ... demonstrating the utility of the grafting of functional peptides with randomized edge sequencesfor optimizing the orientation of the grafted peptide on a scaffold protein. In addition, when the...
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Báo cáo khoa học: Protein transport in organelles: The Toc complex way of preprotein import pdf
... wasdemonstrated); and (c) translocation into the chloro-plast stroma requiring the presence of at least 1 mmATP.Manipulation of nucleotide concentrations and experimental conditions allowed the formation of stable ... preprotein translocation intermediates and the subsequent isolation and identification of components of the associated chloroplast protein import machinery[3–5]. Included among the first components ... proteins isalso tied to a reduction in the expression of the corre-sponding genes [17,65]. Therefore, the extent of the physical involvement of the two receptors, atToc33 and atToc159, in the...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... anchor the protein in the membrane. The position and function of the longC-terminal tail on the other hand remains a matter of controversy [10,11,13–15]. According to one hypothe-sis, the hydrophilic ... undoubtedly the central protein of the tran-slocon. It is not only the largest, most abundant and best studied of all Tic proteins, but also probably the only component involved in translocation steps ... installing components that allow regulation of the translocation efficiency depending on developmental orenvironmental conditions (the regulon).Based on biochemical and genetic evidence, eightproteins...
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Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt
... location-specific isoforms, it is not clear whether the dual-targeted isoform has taken on new functions(neofunctionalization) or whether expression is special-ized (subfunctionalization). Promoter ... from the sequencing of several genomes that the complexity of the proteome exceeds that of the genome in terms of the number of functional units(i.e. there are more proteins than genes). This protein complexity ... regulation of the activity of this complex couldchange the partitioning of dual-targeted proteinsbetween mitochondria and chloroplasts [40,41]. Muta-tion of this site in GR [21], and indeed...
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Báo cáo khoa học: Protein folding intermediates of invasin protein IbeA from Escherichia coli pdf
... correction of an inner filter effect was necessary. The intensity of the fluorescence at the emission maximum was monitored as a function of the increasing concentration of the quencher. The quenching ... 2.0, the protonation becomes saturated and the protein loses its structure and forms the A state. Bothanions and cations will be present in the acidic unfold-ing environment and addition of cations ... b-sheets and, in the presence of 6 m Gdm-HCl, IbeA lost all of the peaks, suggesting the loss of secondary structure. The deconvolution spectrumobtained using the selcon program [29] provides the structural...
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Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx
... positioned in the hairpin loop, the protein termini and the less structured region of residues 34–36 [28],which is in agreement with a possible higher mobility of these regions. The addition of ... rigid protein structure. The least orderedparts of the NMR structure [28] are the N terminus and the loop region. DGP addition leaves the overall pictureunaltered, but, with the exception of segment ... solutecompletely restricts concerted motions of groups on largertime-scales. The influence of DGP addition on the order parameters of RdDg is of the same magnitude as the effect of ligandbinding reported...
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